Ch 13 test review
Terms to know:
selective breeding
hybrid
genetic engineering
genome
recombinant DNA
clone
polymerase chain reaction (PCR)
inbreeding
restriction enzymes
transgenic organism
test cross
gel electrophoresis
DNA fingerprinting
Concepts:
Genetic engineering using recombinant DNA technology:
Like our activity where you mapped out restriction sites on DNA and selected the one
enzyme that could cut out the desired gene and open the plasmid loop
What is the goal of the recombinant DNA technique?
Be able to recognize sites on DNA where restriction enzymes will cut the DNA
Gel Electrophoresis:
What does gel electrophoresis do to fragments of DNA?
What is the charge on a DNA molecule?
The power supply has a positive (+) and a negative (-) wire. Why does it matter how
they are connected to the gel?
What do the bands on the gel represent?
Where do you find large fragments in the gel? Where are the small fragments?
DNA Fingerprinting:
What DNA technology is used to make millions of copies of small samples of DNA?
After the gel has been run with the DNA samples how is a match made?
What are the odds that a DNA fingerprint match is not the correct person?