1.
There are many different views on the ethics of reproductive cloning in humans. Which is a
valid argument against cloning in humans?
A.
It involves the use of donor sperm which is unethical.
B.
It happens naturally when identical twins are conceived.
C.
Only females can be cloned.
D.
The life expectancy of children produced by cloning might be lower than normal.
(Total 1 mark)
2.
What happens to the unfertilized egg used in the cloning process of a differentiated cell?
A.
It becomes fertilized.
B.
Its nucleus is replaced by the nucleus of the differentiated cell.
C.
Its nucleus is fused with the nucleus of the differentiated cell.
D.
Its nucleus is exchanged with the nucleus of the sperm.
(Total 1 mark)
3.
How are plasmids used in biotechnology?
A.
For respiration in prokaryotes
B.
For photosynthesis in eukaryotes
C.
For protein synthesis in prokaryotes and eukaryotes
D.
For gene transfer
(Total 1 mark)
1
4.
What enzymes are used in gene transfer techniques?
A.
Endonucleases and lipases
B.
Ligases and amylases
C.
Ligases and lipases
D.
Restriction enzymes and ligases
(Total 1 mark)
5.
What are the functions of the polymerase chain reaction?
I.
Copy fragments of DNA
II.
Amplify fragments of DNA
III.
Translate fragments of DNA
A.
I and II only
B.
I and III only
C.
II and III only
D.
I, II and III
(Total 1 mark)
6.
What are the possible applications of DNA profiling?
I.
Solving paternity suits
II.
Aiding certain criminal investigations
III.
Identifying people who died last century
A.
I only
B.
I and II only
C.
II and III only
D.
I, II and III
(Total 1 mark)
2
7.
Which enzymes are needed to produce recombinant plasmids that are used in gene transfer?
A.
DNA polymerase and ligase
B.
DNA polymerase and restriction enzymes
C.
Restriction enzymes and ligase
D.
Helicase and restriction enzymes
(Total 1 mark)
8.
The following is a DNA gel. The results are from a single probe showing a DNA profile for a
man, a woman and their four children.
mother
child 2 father child 4
child 1
child 3
origin
I.
II.
III.
IV.
1 2 3 4 5 6
Which child is least likely to be the biological offspring of the father?
A.
Child 1
B.
Child 2
C.
Child 3
D.
Child 4
(Total 1 mark)
3
9.
Why is it possible for a gene from one organism to be introduced and function in a different
organism?
A.
All organisms are made of cells.
B.
All organisms have nuclei.
C.
The genetic code is universal.
D.
All organisms have ribosomes.
(Total 1 mark)
10.
The following is a DNA gel. The results are from a single probe showing a DNA profile for a
man, a woman and their four children.
mother
child 2 father child 4
child 1
child 3
origin
I.
II.
III.
IV.
1 2 3 4 5 6
[Source: The Biology Project, University of Arizona]
Which fragment of DNA is the smallest?
A.
I
B.
II
C.
III
D.
IV
(Total 1 mark)
4
11.
(a)
State one advantage and one disadvantage of genetic modification technology for crop
plants.
Advantage:
........................................................................................................
........................................................................................................
Disadvantage:
........................................................................................................
........................................................................................................
(2)
(a)
advantage: [1 max]
more specific / less random breeding / better yields / pest / herbicide resistance
less likely;
increased resistance to disease / increasing ripening period to allow greater
ease with transport of the crop (eg fruit);
disadvantage: [1]
genes / proteins potentially harmful / allergenic / genes can cross species
barriers / GM crops compete with natural varieties;
2 max
12.
(c)
Describe the technique for the transfer of the insulin gene using E. coli.
(6)
See ppt slide. I don’t have the IB key for this at home.
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