Introduction: Building Proteins from RNA Lab The purpose of this lab was to explore the molecular process of building proteins from the information carried by RNA. The question that we ultimately tried to answer is, “How are proteins built using the information provided by a molecule of RNA?” The hypothesis is if RNA determines the sequence of amino acids in proteins and polypeptides by a two step process, then translation of the mRNA to tRNA takes place at the ribosome in the cytoplasm