Biotechnology Webquest
Website #1: http://www.pbs.org/wgbh/nova/education/body/create-dna-fingerprint.html
Introduction:
1. DNA is unique for everyone. The only exception is if a person has what?
2. What are DNA fingerprints used for?
3. What does a restriction enzyme do?
4. What is agarose gel? _________________________________________ 7. What is electrophoresis?
____________________________________
5. Smaller fragments of DNA move __________________________ than longer strands?
.6 Why do you need to place a nylon membrane over the gel?
7. Probes attach themselves to _____________________
Website #2: https://www.amnh.org/ology/features/perfecttomato/
Click on the Quest for the Perfect Tomato and read the cartoons on tomatoes. Then click on the Food for Thought Link and
read.
8. Give two examples of genetically modified foods.
a. ____________________________________________ b. _________________________________________________
Website #3: http://learn.genetics.utah.edu/content/cloning/
Open the link and view each of the sections under "Cloning in Focus". For each section, answer the questions.
What is Cloning?
9. Who is Dolly? ______________________
10. When a zygote divides into to separate cells, it is called: _______________________________
11. Somatic cells are also called _________________________
12. In order to clone a gene, a gene is inserted into a _________________________
13. In order to create an embryo from a somatic cell, the donor egg cell must have its ___________________ removed.
Click and Clone
14. List all the materials needed to clone a mouse.
15. Place the following steps in the box to the right correct order.
________ Stimulate cell division
________ Deliver baby
________ Remove and discard the nucleus from the egg cell
________ Isolate donor cells from egg donor and germ cell donor
________ Transfer the somatic cell nucleus into the egg cell
________ Implant embryo into a surrogate mother
16. There are two time gaps in the process of cloning. What are they? (ie. what do you have to wait for?)
17. What color with the cloned mouse be? _____________ What is the name of this mouse? _____________________
Why Clone?
18. Why is cloning extinct animals problematic?
19. What are some reasons a person might want to clone a human?
Cloning Myths
20. Briefly describe in your own words, why CC the cat was not identical in color to Rainbow, even though she was a clone.
21. What is "nature vs nurture"?
Is it Cloning or Not?
22. For each of the following scenarios, indicate YES (it is cloning) or NO (it is not cloning)
___________Sperm taken from a mole goat is combined with a female's egg in a petri dish. The resulting embryo is implanted
into the female's uterus to develop
___________A sheep embryo, composed of 16 cells, is removed from the mother's uterus and separated into indivudal cells.
Each cell is allowed to multiply, creating 16 separate embryos, which are then implanted in different female sheep
to develop to maturity.
___________A cow with many desirable traits is stimulated with hormones to produce a number of egg cells. Each of these
eggs is fertilized and implanted into a surrogate mother.
___________ In vitro fertilization
___________ Cell nuclei from an extinct wooly mammoth are placed into enucleated cow cells.
What Are the Risks of Cloning?
23. What is one reason why cloning animals has such a high failure rate?
24. What is a telomere and how does it affect cloned animals?
What Are Some Issues in Cloning?
25. Pick one of the questions to ponder. Write a paragraph below on your thoughts and opinions.
Gene Therapy: Go to website http://learn.genetics.utah.edu/content/genetherapy/intro/ and answer the questions below:
1. How does gene therapy work?
2. Analyze the case studies on cystic fibrosis to the right, and answer the following questions:
a. What are the symptoms of cystic fibrosis?
b. What type of mutation originates cystic fibrosis?
c. Will gene therapy cure cystic fibrosis?