How to run a PT test
A/ Reagent Preparation
• 🥤 Transfer the entire contents of one vial of Solvent (R2) into one vial of
Néoplastine® (R1) of the same kit.
• 🕕 Allow the reconstituted reagent to stand at room temperature (18-25°C) for
30 minutes.
• ➰ Swirl the R1 vial gently to obtain a homogeneous suspension.
B/ Analyser Preparation
•
•
While the analyser is off, hold down
then push
Here you can set the ISI and MNPT values, using buttons 2, 3, and 4 as indicated
on the screen
•
Then you can switch the analyser on
•
Then wait for it to reach 37°
The reconstituted Néoplastine® needs to be incubated in the 37°C reagent
spot on the analyser, not filled higher than the top of the space
•
Place cuvettes in the cuvette incubation locations, with a metal ball in each
cuvette for at least 3 minutes to heat the ball up
•
Then you need to choose the incubation time for the PT test to 120s by
Holding down
then pushing
until the desired incubation time is
Visible
Director: K N Dodkins
C/ Test Procedure
•
🔥 In an appropriate cuvette with ball at 37 °C, pipette 100μl plasma (standard,
patient’s or control) at the 12o’clock position in the cuvette, choose the test by
pushing
and then
to choose the PT test which will start to incubate
the sample for 120 seconds at 37°C.
• When there is only 20 seconds left of the incubation, the analyser will indicate
this every 5 seconds by beeping once, move the cuvette to the measurement well
• A longer tone indicates the end of the incubation, then a “start” appears on the
screen. You have 5 seconds to active the measurement mode – activate the
measurement mode by pushing the
button
•
🥤 Add 200μl of pre-warmed Néoplastine® at the 6 o’clock position and
simultaneously start measurement for clot formation by pushing the
button.
The pipetting of the pre-warmed Néoplastine® at the exact same time as you push the
button is very important, as this indicates the start of the clotting time
•
Record the time, in seconds (T)/Ratio (R)/INR (I), this flashes on the
screen
D/ Stability
Once reconstituted, R1 remains stable for:
Stability: 8 hours at 37 °C
Stability at room temperature: 48 hours at 20 ± 5 °C
Stability in fridge: 8 days at 2-8 °C.
Applications support: Bronwyn Urquhart - +27 78 566 3206
Director: K N Dodkins