Determination of cholesterol
in clinical blood samples
The Mass Spectrometry Approach
https://www.chemistryworld.com/podcasts/cholesterol/3005737.article
Presented by Chen J., Gaju S., Lasiri A., and Serban A.
Concordia University
March 20222
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What is cholesterol?
Cholesterol is a waxy type of fat, or lipid!
Have you had your cholesterol checked recently?
Naturally in your body: LDL (low-density lipoprotein), makes
up most of your body's cholesterol; HDL (high-density
lipoprotein), can lower your risk for heart disease and stroke.
Triglyceride: A fat produced by the body from sugar, alcohol
or other foods.
Prostate Cancer
Heart disease
Osteoporosis
Hypertension
Stroke
Life threaten!
https://www.kindpng.com/imgv/iJwJbRJ_sick-person-free-people-cliparts-clip-arttransparent/
https://dilwalk.ca/how-does-saturated-fat-affect-cholesterol/
https://www.publichealthnotes.com/10-differences-between-ldl-and-hdl/
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What methods do we use to
detect cholesterol in the blood?
3 main methods(ranking by time invention)
1. Modified Abell-Kendall method(reference method by
CDC）
2. Fluorometric-enzymatic assay
3. Mass spectrometry
-Gas chromatography-Mass
spectrometry( routine cholesterol determination.)
-Gas chromatography–Isotope dilution-Mass spectrometry
(standard reference for total blood cholesterol by National
Institute of Standards and Technology )
scheme of L-B reaction https://doi.org/10.1016/j.jfda.2018.09.001
https://www.cdc.gov/cholesterol/ldl_hdl.htm
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