INSTITUTE OF NUTRITION AND FITNESS SCIENCES COMPREHENSIVE GUIDE ABOUT PROTEIN - ASMITA SHAH www.infs.co.in Page - 1 Protein is probably the most underestimated and misunderstood nutrient today. In western countries, a majority of people consume enough protein but their choice of protein makes them susceptible to overall high calorie/fat or sodium consumption. Instead of looking for healthier preparations, they are entirely shunning non-vegetarian diets and adopting plant-based diets. In developing countries like India, where a large part of our diets is carbohydrates, there is a severe def iciency in protein intake in the general population. The myths about how too much protein is harmful to the body further exacerbates the issue. If you are not meeting the minimum requirement, you are far f rom the scenario where you should be worrying about exceeding the limit. We hope with this short ebook, you will receive a crash course in all the facts related to protein. You can then assess for yourself if you need protein, how much you should eat, and where you can get it f rom! Learning happens one day at a time, and we are happy to be a part of your journey. Happy Reading! Yours in learning and health, Jyoti Dabas Founder and CEO, INFS Page - 2 INFS VISION “ To shape our students into world-class f itness professionals who are knowledgeable, inspiring, and compassionate. We, along with our students, will serve our communities to make health education available to all. ” Page - 3 Table of Contents 01. Protein - A Building Block of Life 06 02.Effects of Insufficient Dietary Protein Consumption 08 03. Health Benefits of Protein 10 04. What Are Proteins Made Of? 12 05. Types of Protein 13 Page - 4 06. Protein Bioavailability and Quality 15 07.Are Protein Supplements Necessary? 16 08. Myths About Proteins! 18 09. Our Courses 20 10. References 22 Page - 5 PROTEIN - A BUILDING BLOCK OF LIFE Page - 6 Protein is a part of every cell in the body that helps it to build and repair cells and tissues. In the Greek language, it is known as proteios, which means “primary”. It is a major component of the skin, muscle, bone, organs, hair, and nails. Research reveals that most age/sex groups appeared to consume more protein than the respective estimated average requirement, while a significant percentage of adolescent females and older women appeared to have inadequate protein intake. Protein intake averaged 56 ± 14 gms/day in young children, increased to a high of ≈91 ± 22 gms/day in adults aged 19–30y, and decreased to ≈66 ± 17 gms/day in the elderly in the US. In India, people consume diets that are rich in carbohydrates which constitute approximately 70-80% of their food. Protein sources that are consumed by Indians include dairy products, pulses, chicken, and eggs in the case of non-vegetarians, but in a limited quantity. In India, it has become a cultural practice to eat protein only on a few days of the week or on some special occasions. The reality is that given its importance in our overall health, it should be consumed every single day. It took this pandemic for people to understand the importance of building their immunity, and protein is vital for the same. Besides, your hair and nails also grow stronger because of protein. The Indian Market Research Bureau’s 2017 report states that protein deficiency among Indians stands at more than 80 percent, measured against the recommended 60 gm per day. Vegetarians are most likely to be deficient in protein intake. Hence, including protein sources in daily life will fill the gap. One gram of protein provides around four calories, but it provides more satiety that makes us feel fuller than carbohydrates and fat over a longer period of time. A diet that is rich in protein lowers appetite, in turn leading to possible weight loss by reducing the intake of calorie-dense foods. Page - 7 EFFECTS OF INSUFFICIENT DIETARY PROTEIN CONSUMPTION Page - 8 When dietary protein is not sufficient, the body breaks down its skeletal muscles to preserve more important tissues and body functions that lead to muscle loss. Our body, skin, hair, and nails are made up of protein. Low intake of protein may lead to flaking, thinning of hair, hair loss, brittle nails, depigmentation of the skin, lowered immunity, and other issues. Increasing dietary protein can help in slowing down the muscle loss associated with aging [2]. How much protein do you need every day? Protein is one of the most misunderstood nutrients in India with 93 percent of Indians being unaware of their ideal protein requirements. The current Recommended Daily Allowance (RDA) of protein for adults is 0.8 gm per kg [7]. The RDA is the minimum amount we need to meet our basic nutritional requirements. Current findings suggest that a general recommendation for protein would be 1.2- 2.0 gm per kg of body weight (0.8 - 1 gm per pound of bodyweight) depending on the activity, training, and diet history of an individual. Conditions that may increase the requirement of protein intake [3]. ● Aging ● Injury and Illness ● Obesity ● Diabetes ● Osteoporosis ● Resistance Training Page - 9 HEALTH BENEFITS OF PROTEIN Page - 10 1) Build More Muscle Mass Muscles are primarily made up of protein. When we perform any physical activity, protein requirement increases. Consuming sufficient protein leads to more muscle tissue than we are breaking down, which leads to more muscle over time [1]. There is a progressive loss of muscle mass with aging. Significant loss of muscle mass with aging is termed sarcopenia. The combination of resistance training along with sufficient intake of protein has shown improvement in muscle mass [2]. 2) Improved Body Composition In order to lose fat, we need to be in a calorie deficit. A sufficient intake of protein will help in retaining maximum muscle mass and, thus, improve body fat and muscle mass ratio [4]. 3) Improved Satiety Protein keeps you full for a longer period of time as it regulates the hunger hormone (Ghrelin) and increases the levels of satiety hormone (Peptide YY) [5]. 4) Improved Bone Health In addition to calcium, in the presence of an adequate supply of vitamin D, dietary proteins represent key nutrients for bone health and, thereby, function in the prevention of osteoporosis, a condition in which bones become weak and brittle [6]. Page - 11 What Are Proteins Made Of? Protein is made up of long chains of amino acids. There are roughly 500 amino acids occurring in nature but only 20 amino acids occur in proteins. They are categorized as essential, non-essential, and conditionally essential. AMINO ACIDS Essential Amino Acids Conditionally Essential Amino Acids Non Essential Amino Acids The body creates 11 of the 20 amino acids on its own. These are the non-essential amino acids. We do not need to consume it from food sources. Out of these, 9 are essential amino acids and of those nine, three are branched-chain amino acids (BCAAs) namely leucine, isoleucine, and valine. Leucine is the most researched amino acid and is very important as it stimulates muscle growth, helps in healing skin and bones. Intake of sufficient amounts of good quality and complete protein like meat, eggs, whey, soy, etc provides enough of these amino acids. Supplement companies in the market sell a wide range of BCAA. BCAAs are no longer used by bodybuilders or athletes but are now consumed by the general population as well. There is inconclusive research on whether taking these supplements in isolation helps in muscle recovery or growth. If you are having a balanced diet with an optimal protein intake, you may not need BCAA supplements. Page - 12 Types of Protein Protein can be found in plants as well as animal-based foods. The main difference between these sources is the amino-acid profile. Complete and Incomplete proteins Complete vs Incomplete Proteins Definition Nine Essential Amino Acids Food Source Providing all Essential Amino Acids Complete Proteins Incomplete Proteins Complete proteins are the proteins that contain all essential amino acids in sufficient quantities Incomplete proteins are the proteins that lack one or more essential amino acids in sufficient amounts All nine essential amino acids are present Lack one or more of the nine essential amino acids Available in many Available in most animal products (fish, plant products meat, poultry, eggs, etc.) and in soya beans and quinoa One animal product All essential amino alone can provide all acids can only essential amino acids be obtained by consuming a variety of plant products Page - 13 Animal Protein Animal sources are complete sources of protein, having all the essential amino acids. These are rich in certain nutrients which include heme-iron, vitamin B12, vitamin D, DHA, and zinc. Plant Protein Plant protein is a type of protein source that has a plant origin. It is an incomplete protein, which lacks one of the several essential amino acids. Plant protein contains phytonutrients such as antioxidants and non-heme iron, which is less absorbable by the body. Nonvegetarian Source per 100 gm Vegetarian Source per 100 gm Egg (P: 6 gm F: 5 gm C: 0 gm) Soya (P: 52 gm F: 0 gm C: 33 gm) Chicken (P: 24 gm F: 7 gm C: 0 gm) Paneer (P: 14 gm F: 25 gm C: 2 gm) Tilapia (P: 26 gm F: 3 gm C: 0 gm) Whey (P: 24 gm F: 1 gm C: 3 gm) Salmon (P: 20 gm F: 13 gm C: 0 gm) Cheese (P: 20 gm F: 26 gm C: 2 gm) Prawns (P: 20 gm F: 2 gm C: 2 gm) Milk (P: 6 gm F: 5 gm C: 0 gm) Beef (P: 16 gm F: 19 gm C: 0 gm) Lentils (P: 21 gm F: 1 gm C: 60 gm) Red Meat (P: 20 gm F: 24 gm C: 0 gm) Tofu (P: 12 gm F: 6 gm C: 2 gm) Turkey (P: 20 gm F: 24 gm C: 0 gm) Greek Yogurt (P: 6 gm F: 5 gm C: 0 gm) Page - 14 Complementary Proteins To give you an idea about it, let’s talk about lentils: There are different varieties of lentils and each has its own nutritional content. On average, every 50 grams of lentils contain only about 10 grams of protein and 25 grams of carbohydrates. Lentils don’t provide complete proteins and, hence, it is an incomplete source of protein and is high carb. To make it a complete protein, combine your lentils with whole grains and with different seeds that give you the required essential amino acids. The combination of grains and legumes works well if you want a variety of amino acids in a single meal. Here is a list of some food combinations that make a complete protein: • Legumes with grains, nuts, seeds, or dairy • Grains with dairy • Dairy with nuts • Dairy with nuts/seeds and legumes Protein Bioavailability and Quality Protein bioavailability means how well the body is able to utilize and digest it. Protein digestibility-corrected amino acid score (PDCAAS) is a score that explains the quality of the protein based on the amino acid profile. It is the most widely used and trusted method to check protein quality. Protein items can contain complete protein. But, due to digestibility factors, it can still have PDCAAS<1. Egg, whey protein, milk have PDCAA scores equal to 1. Animal-based protein sources have a higher concentration of essential amino acids. Whereas, plant-based sources have lower protein quality. The digestibility of plant-based protein (except soy) appears to be less than that of animal products, indicating that vegans may benefit from higher protein intake than the meat-eaters. Various plant-based protein sources can be combined to achieve high-quality protein feedings. Page - 15 Are Protein Supplements Necessary? To get an answer to this simple question, counter-question yourself. DO YOU EAT ENOUGH PROTEIN FOR THE DAY? If completing your daily required protein intake is difficult, then protein supplements can be a blessing in disguise. A lot of people, nowadays, are consuming protein supplements in order to meet their protein needs. There are different types of protein supplements like whey, casein, soy protein, protein pancakes, etc. Whey Protein, the most commonly used one, is a dietary supplement derived from milk. The digestive enzymes and flavors are added to the raw whey protein which is derived from the milk to add taste. It is one of the highest quality protein sources available and is also a fast-digesting protein. There are three types of whey protein supplements available in the market, viz. concentrate, isolate, and hydrolyzed. The difference between them is mostly insignificant - an isolate is a purer form of concentrate while hydrolyzed is a Page - 16 more refined form of whey isolate. Feel free to choose any type but if you’re lactose intolerant, then stick to whey protein isolate or hydrolyzed. It is not compulsory and necessary to take protein supplements. But, if you are unable to complete the protein requirement, including whey protein will help. Follow the below tips while selecting a whey protein supplement: • Read and understand the nutrition label of the supplement • Protein supplements should contain 20-25 gm protein in each serving • Carbohydrates and fats should be less than 5 gm in a scoop • The serving size of the scoop shouldn’t be more than 35 gm • 11% of each 25 gm protein should be leucine, which means 2.75 gm of leucine per 25 gm. It should not be less than 2.5-2.75 gm • Make sure it doesn’t contain “proprietary blends”. • 25% of whey should be BCAAs, which means 6.25 gm per 25 gm of protein. • Always buy supplements from authentic sellers, and get your product authenticated by visiting the official website of the product. Page - 17 MYTHS ABOUT PROTEINS! Page - 18 Myth 1: Vegans and Vegetarians need supplements. Most vegans and vegetarians appear to consume less protein than omnivores. The protein quality is another concern as most plant-based sources are incomplete, missing important amino acids, and typically less Branched-Chain Amino Acids (BCAA) than the animal-derived equivalents. To complete the protein requirement of the day from a high-quality source, supplementation might be beneficial but not mandatory. Myth 2: Protein supplementation is important for those building muscle mass. A supplement is introduced when you are unable to meet the requirements from whole food sources. Protein supplements are helpful even when the goal is not to build muscle mass. A typical Indian vegetarian diet is not very rich in protein. Hence, not just bodybuilders, but any person who is unable to meet the protein requirement through their diet may consume whey protein. Myth 3: Protein damages your kidney. Several studies have shown that a high protein diet does not show any adverse effect on kidney functioning in a healthy individual. People with existing renal disorders should take a low protein diet. Myth 4: Protein is protein. Not all protein sources are equal. As discussed above, there are different types of protein, namely complete, incomplete, and complementary protein, depending on the amino acid profile. Myth 5: Is it important to take protein when one is not training? Yes! It is important to complete the protein requirement of the day even on non-training days. The body needs protein to perform various functions. Page - 19 OUR COURSES Page - 20 INFS aims at delivering comprehensive knowledge backed by research through all the INFS courses including the Diploma in Nutrition and Fitness and the Calisthenics Trainer Certification. INFS Diploma in Nutrition and Fitness • Research-backed & evidence-based curriculum • University-grade diploma valued at 40 credits • Completely online course • Multiple learning aids • Hands-on experience with INFS Free Training Program INFS Calisthenics Trainer Certification • One-of-a-kind course to become a dedicated calisthenics instructor • Educates about human anatomy, physiology, biomechanics, & kinesiology • Guidance over assessing clients & designing training plans • Skill sessions for understanding progression techniques • Instructions regarding technique and training protocol Page - 21 References: 1.Tang, J. E., & Phillips, S. M. (2009). Maximizing muscle protein anabolism: the role of protein quality. Current Opinion in Clinical Nutrition & Metabolic Care, 12(1), 66-71. 2.Morton, R. W., Murphy, K. T., McKellar, S. R., Schoenfeld, B. J., Henselmans, M., Helms, E., ... & Phillips, S. M. (2018). A systematic review, metaanalysis and meta-regression of the effect of protein supplementation on resistance training-induced gains in muscle mass and strength in healthy adults. British journal of sports medicine, 52(6), 376-384. 3.Deutz, N. E., Ashurst, I., Ballesteros, M. D., Bear, D. E., Cruz-Jentoft, A. J., Genton, L., ... & Prado, C. M. (2019). The underappreciated role of low muscle mass in the management of malnutrition. Journal of the American Medical Directors Association, 20(1), 22-27. 4.Wirth, J., Hillesheim, E., & Brennan, L. (2020). The Role of Protein Intake and its Timing on Body Composition and Muscle Function in Healthy Adults: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. The Journal of nutrition, 150(6), 1443-1460. 5.Leidy, H. J., Clifton, P. M., Astrup, A., Wycherley, T. P., WesterterpPlantenga, M. S., Luscombe-Marsh, N. D., ... & Mattes, R. D. (2015). The role of protein in weight loss and maintenance. The American journal of clinical nutrition, 101(6), 1320S-1329S. 6.Bonjour, J. P. (2011). Protein intake and bone health. International Journal for Vitamin and Nutrition Research, 81(2), 134. 7.Wu, G. (2016). Dietary protein intake and human health. Food & function, 7(3), 1251-1265. Page - 22 Email: support@infs.co.in Phone: 020-71968000 Time: Mon - Sat 9 am to 9 pm IST Sun 9 am to 5 pm IST www.infs.co.in Page - 23