HRT 220 FACTORS IN PREVENTION OF CANNABIS DISEASES AND PESTS WEEK 2 ABIOTIC FACTORS An abiotic factor is a non-living part of an ecosystem that shapes its environment. In a terrestrial ecosystem, examples might include temperature, light, and water. In a marine ecosystem, abiotic factors would include salinity and ocean currents. Abiotic factors affect the growth, yield and populations of microorganisms living on plants, as well as vector populations, impacting the spread of plant pathogens. We must pay attention to abiotic factors in the environment so that we do not create a favorable environment. ABIOTIC FACTORS INFLUENCING CULTIVATION OF CANNABIS The relative humidity of the air The temperature of the air The movement of the air The temperature, pH, and amount of water The pH of the media and leaf surface The temperature of the media What IPM tactics can we use to make the environment unfavorable to pathogens? Air DESIGN FACTORS IN PREVENTATIVE MAINTENANCE Temperature Water Media Growing in indoor spaces provides the luxury of being able to control your atmospheric conditions. AIR The air in the room needs to be managed in order to create the best environment to ward off pests and diseases. Wind strengthens stems and causes evaporation which keeps things dry and helps to reduce favorable conditions for molds. Wind needs to be mimicked indoors. Humidity is the amount of water vapor present in the air. Water vapors are the gaseous state of water and are invisible. Humidity can be measured by three ways: 1. Absolute humidity is the water content of air at a given temperature. It is measured in the gram per cubic meter. 2. Relative humidity is expressed in percentage, and it measures the current absolute humidity relative to the maximum humidity for that temperature. 3. Specific humidity is expressed as the ratio of the mass of the water vapors to the total mass of moist air parcel. The glass is at 50% of its capacity. That is similar to relative humidity. If RH was at 50% the air would be at 50% of its capacity to hold water. At 100%, condensation occurs. 6 ounces is 50% of 12 ounces and 8 ounces is 50% of 16 ounces. 6:12, and 8:16 can be thought of as the specific humidity. When temperature increases it means energy increases which allows more water molecules to release their bonds and float with air molecules. As temperature rises so does the ability for air to hold more water. At 20 C, the air can only hold up to15 grams of water. At 40 C, the air can hold up to 50 grams of water. 15 grams of water vapor at 20 C creates 100% RH, 15 grams of water vapor at 40 C creates 25% RH. When Night falls and the colder air can’t hold the water, condensation forms. •Stomatal closure •Reduced CO2 uptake RELATIVE HUMIDITY EFFECTS TRANSPIRATION AND NUTRIENT ABSORPTION If the temperature in your grow room or greenhouse lowers, you need to bring humidity down with it or the dewpoint will be reached and condensation will form. Condensation creates a favorable environment for some diseases and pests. Transpiration increases at lower RH. With low RH, plants need more water and less fertilizer. A high amount of nutrients with low RH can cause salt build up and lock-out because it draws nutrient filled water through the plant at a fast rate, bringing high amounts of nutrients into the tissues of the plant. Transpiration slows with high RH. With high RH, plants need less water. Increases heat load of plants, stomatal closure, and reduces CO2 uptake WHAT’S THE CORRECT HUMIDITY LEVEL NEEDED? To figure out where you want the relative humidity level at in your grow room, you must relate it to the temperature. The higher the temperature in the room, the higher you want the humidity in the room. The higher humidity slows the rate of transpiration so that large doses of nutrients do not get pulled into the plant. If you don’t lower the rate of transpiration, than the high temperatures will cause stomates to close in order to slow transpiration. With no water vapor being released on the surface of the leaf, the temperature of the leaf surface with rise and cause curling. The lower the temperature in the room, the lower you want the humidity in the room in order to avoid condensation forming on the plant. WHAT’S THE CORRECT HUMIDITY LEVEL NEEDED? Growers have the benefit of a premade chart that they can consult to figure out what their ideal temperature and humidity should be at to create the ideal transpiration rate, which will help the plant stay healthy with the right amount of nutrients, water and cool leaf surface. The first and best defense against plant diseases is a healthy plant, VAPOR PRESSURE DEFICIT (VPD) VPD measures the difference between the pressure inside the leaf and the pressure of the air outside. Gives us an idea of how easy it would be for the plant to transpire. A high VPD means the plant is transpiring quickly – too high and it pulls nutrients through too fast A low VPD means the plant is transpiring slowly – too slow and moisture forms, and low amount of nutrients are up taken. Measured in Kilopascals (kPa) or Millibars (Mbar) 1 kPa = 10 Mbar Leaf temperature has an effect to VPD. Leaf temps should read below your room temp by 2 C or 3.5 F difference. If it is equal to or above, lights are too close. Unit of measurement: Kilopascal (kPa) Millabars (Mbar) 1 kilopascal = 10 millabars In flower you want to make lower relative humidity. This will create an unfavorable environment for mold. THE RISKS OF HIGH HUMIDITY When conditions are too humid, it provides a favorable environment for: The growth of mold, mildew, and bacteria Conditions like root or crown rot. Pests, such as fungus gnats, whose larva feed on plant roots and thrive in moist soil. However, spider mites do not like humidity above 60% AIR MOVEMENT TO REDUCE AND PREVENT PEST AND DISEASE Moisture is generally needed for spore germination, the multiplication and penetration of bacteria, and the initiation of infection. Temperature, gas exchange, and humidity can all be affected positively through the air circulation and exchange. Circulation is used to keep things moving and fairly equal. Air exchange is used to control when the temperature or humidity gets out of hand. Circulating and exchanging air replaces the depleted gas areas at the leaf surface with normal air to insure carbon dioxide and oxygen are available for basic life processes. AIR MOVEMENT Mold, mildew, and insects prosper in stagnant, humid conditions. Airflow from fans creates a steady breeze, protecting against harmful mites, fungus, and diseases. Even at low humidity levels, if you have no air flow and your grow room is stagnant, bud rot can proliferate fairly easily. Provide as much air flow as possible without actually damaging the plant. Air flow systems should operate continuously in greenhouses during periods of high humidity, which often occur at night and during rainy days. Exhaust fans, when present, can be used to circulate air in greenhouses. https://www.sciencedirect.com/science/article/abs/pii/0261219484900589 Ventilation in high tunnels is manipulated by opening sidewalls, endwalls, and/or ridge vents, if applicable. AIR MOVEMENT AND PROPER PLANT SPACING To decrease humidity provide good air circulation by leaving space between plants. If plants are close make sure the breeze is strong enough to penetrate the canopy. Genetics/ lighting/ humidity/ air circulation/ pruning all influence appropriate spacing INSECTICIDAL LIGHTS AND FANS Fans help lower humidity and temperatures in the growing area. Fans inside the growing area are important for eliminating microclimates. Having microclimates increases your chances of providing favorable conditions for pests and diseases. Insecticidal lights kills fungal spores, bacteria, and insects that come in contact with it. They are often installed in the vents bringing air into the grow room Fans are rated by CFMs. The appropriate amount of CFMs is equal to the area of the grow room. TEMPERATURE AND PESTS AND DISEASE High humidity and low temperatures encourage some plant diseases. Maintain optimum temperature range 70 to 85 F. Genetics influence temperature tolerance. Afghan-cooler Indica-hotter High and low temperatures weaken plant defense responses Lessen temperature difference between night and day to reduce dew condensation. TEMPERATURE AND PESTS AND DISEASE Temperature is one of the most important factors shaping the nature of plant pathogen interactions, as well as the normal development of healthy plants. High temperatures facilitate the spread of tobacco mosaic virus by weakening the plant defense responses. Powdery mildew proliferates at higher temperatures 68-86 F, but begins to die above 90 F Botrytis mold proliferates at lower temperatures 30-68 F, but stops growth at 89 F, and dies above 105 F Fusarium proliferates at a wide range of temperature, optimal growth is 59-86 F, growth stops above 107 F Use a clean water source. Infected water can be sterilized by passing it through a UV light Reverse Osmosis will remove pathogens, dissolved solids, and harmful chemicals. If growing prebiotically, chlorine will kill any beneficial microbes. Be sure to filter water. WATER Eliminate any leaks in the garden space and make sure drainage is complete. Puddled water can lead to algae growth and support fungus growth. AVOID OVERWATERING OR UNDERWATERING Careful watering is key to avoiding the growth of fungi and molds. Most Fungi responsible for dampening off survive in overly wet environments. Be sure planting mix drains well. Plant seeds one eight to a quarter inch deep if sowing directly, seeds planted deeper are more vulnerable to dampening off. Drought to the point of wilt weakens plants, making them more vulnerable to disease. Roots need a constant supply of oxygen and water. This is achieved with moist but not wet conditions. BALANCE THE PH Hidden pH problems slow your cannabis plants’ growth, make your plants sick, decrease yields and potency, and can even kill your cannabis plants Fungi thrive in pH environments lower than 7 with optimum being 4.5 – 6 Raising pH on leaf structure can fight or prevent a fungal infection Potassium hydroxide (lye) and Potassium bicarbonate (baking soda) are often used to increase pH. A pH of 8 is sprayed on foliage to create an inhospitable environment for fungi. PH LEVELS IN THE SOIL Cannabis grown hydroponically or without soil needs an even lower pH than a soil grow. If you grow in soil, the optimal pH level for the root zone is between 6.0 and 7.0. If you grow in soilless or hydroponics, the optimal pH level for the root zone is between 5.5 – 6.5 allow the pH level to fluctuate across the acceptable range to support nutrient uptake. For example, in hydro, calcium and magnesium are mostly absorbed at pH levels above 6, while other nutrients like manganese prefer a slightly lower pH. Pests and diseases not only live on the plant, but they can also live in the growing media. Raised beds can prevent gophers, moles, voles, and rats from MEDIA eating your plants in the ground. Placing wiring at the bottom of beds in the ground can help ward off pests. Healthy soil contains the right balance of nutrients, microorganisms and other beneficial organisms that help fight off disease and pests. Mulches, crop rotation, irrigation cleaning, all help to reduce pest problems. CONTROL PEST AND DISEASE BY KEEPING PLANTS HEALTHY Get to know pests desired environments and create unfavorable conditions. Enzymatic reactions are limited by temperature. Low temperatures slow enzymes which lowers the defenses of the plant. High temperatures can retard photosynthesis and create nutrient deficiencies or toxicity.