Assignment Pack National 5 Biology Science Faculty N5 and Higher Assignment Write Up Checklist Name of pupil: ___________________________ Class: __________ Producing the report: The report must be all your own work When producing your report, you will be supervised by your teacher at all times. You have 1 hour and 30 minutes to complete your report. Your teacher cannot provide you with feedback or tell you how to improve your report. Report Stage Checklist: Instructions for candidates Underlying Science research notes relevant to your aim Experimental method Raw experimental data Internet or literature data/information Reference to the Internet or literature data/information I have checked that the materials to be used by candidates in the report stage are not previously prepared drafts. Teacher Signature: _____________________________ Date: _______________ You will pick one of 3 experiments to do. How sugar effects respiration How pH effects enzyme activity How water concentrations effects osmosis Main stages for assignment Aim (1), Using your experiment title you will need to come up with an aim Underling biology (3) You need to find information on your chosen topic. You will be provided with some questions to help your research. This will be written in paragraphs You will get a chance to look at examples of this. Experiment (10) For your experiment you will need to follow the instructions. You will then Wright a simple explanation of the method. Following this you will make a table based on your results. You will then use this table in the write up stage and calculate an average of your results. You will then need to make a graph of your averages Internet research (2) You will then need to find information form the internet or textbooks to compare your data with another set of data. There are some sources provided or you can use the Internet to find your own You need to make a reference of you Internet data. Analysis (1), you will need to compare your data to the Internet data Conclusion (2) Next you will make conclusions based on your experiment comparing it to your internet research and relating this to your aim Evaluation (2) Finally you will look at ways to improve your experiment. Experiment 1 Respiration 1. The equations for aerobic respiration? 2. The equations for fermentation in yeast? 3. What chemical controls respiration? 4. What is the main difference between aerobic respiration and fermentation? 5. Describe the stages of aerobic desperation? 6. Describe the stages of fermentation? 7. Where does aerobic respiration take place? 8. Where does fermentation take place? 9. What are the limiting factors for respiration? 10. What are the limiting factors for fermentation? 11. How much ATP is formed in Arabic respiration? 12. How much ATP is formed in fermentation? 13. How does temperature effect rates of respiration? 14. Why does temperature have this effect? Equipment Fresh yeast Boiling tubes 10 % Sugar solution Flour stirring rod Weighing boats Balance Water bath Measuring cylinder Cling film Method 1. Weigh 120g of flour 2. Add 8g of fresh yeast 3. Mix in 80ml of sugar solution 4. Mix with a glass rod until it stats to become solid 5. Weigh out 16x 10g balls of dough 6. Shape into a torpedo shape and roll in flour 7. Place in boiling tube and tap down with stirring rod 8. Mark the height of the dough 9. Cling film top of boiling tube and seal with tape 10. Place in water baths at chosen temperatures. 11. Leave for 24 hours (48 hours). 12. Measure distance between top of dough and original height Experiment 2 Enzymes 1. What is the function of an enzyme 2. Name and describe the 2 types of reaction carried out by an enzyme 3. Draw a diagram to show how enzymes work on substrate to make a product 4. What is meant by optimum conditions 5. How do optimum conditions relate to body temperature 6. How does pH effect the shape and function of enzymes 7. What is the optimum pH for catalase 8. What is the equations for catalase braking down hydrogen peroxide 9. What type of reaction does catalase carry out. Equipment Selection of different pH solutions Catalase Hydrogen peroxide Detergent Boiling tubes Ruler Stop clock Method 1. Add 10ml of hydrogen peroxide to the boiling tube 2. Add 5 drops of detergent to boiling tube 3. Add 5ml of pH buffer to boiling tube 4. Add 2ml of catalase to test tube 5. Repeat for each pH 6. Run the experiment for 5 minutes 7. Measure the height of the foam Experiment 3 osmosis 1. Describe the structure of the cell membrane. 2. Draw a diagram to show osmosis in a beaker 3. What is a concentrations gradient 4. What is passive transport 5. What is osmosis 6. Does passive transport need energy 7. What type of transport need energy 8. What happens to plant cells in high water concentrations 9. What happens to plant cells in low water concentrations 10. What would happen to the mass of a cell that has become plasmolysis 11. What would happen to a cell that has become turgid? 12. What would happen to an animal cell in a low water concentration 13. What would happen to animal cell in a high water concentration? 14. Why do different things happen in plant and animal cell when in different water concentration? Why? 15. If a solution has a high salt concentration what can you say about the water concentration 16. If a solution has a low salt concertation what can you say about the water concertation? Equipment Potato Ruler Boiling tubes Balance Cork borer Range of sugar solutions Paper towel Knife Method 1. boar out 3 potato cores 2. cut each to 5cm weigh each core 3. place in boiling tubes 4. cover with 20 ml of sugar solution 5. repeat step 1-4 for each sugar concentration 6. leave for 24h 7. remove from sugar solution 8. blot dry using paper towel 9. weigh each potato core Appendix 1 Source Information Experiment 1 Respiration http://ebooks.dynamiclearning.co.uk/prod_content/extracted_books/9781471840470/OEBPS/c12.htm http://www.westminster.edu/about/community/sim/documents/SEffectofTe mperatureonFermentation.pdf (https://www.quora.com/What-is-the-effect-oftemperature-on-the-rate-of-budding-of-yeast) https://www.researchgate.net/figure/Effect-of-temperature-on-yeast-growth-A-Specific-growthrates-of-thermotolerant-and_fig1_281820629 https://nanopdf.com/download/effect-of-temperature-5ae3db37e1ff9_pdf http://www.biologydiscussion.com/respiration/aerobic-respiration/factors-affecting-aerobicrespiration-8-factors-plants/15206 https://www.researchgate.net/figure/Effect-of-temperature-on-free-and-immobilized-yeastgalactosidase-activity-t-30-min_fig5_247177260 Experiment 2 Enzyme https://alevelnotes.com/notes/biology/biological-molecules/enzymes/factorsaffecting-enzyme-activity https://www.researchgate.net/figure/Graph-showing-the-pH-activityrelationship-for-catalase-4-mg-ml-during-lyophilization_fig3_232257560 https://www.researchgate.net/figure/pH-Optimum-of-catalase-from-camelliver-DEAE-Sepharose-fraction-The-reaction-mixture_fig3_267960735 http://www.worthington-biochem.com/introbiochem/effectspH.html https://www.tes.com/lessons/VUCPcE9FachHaA/enzyme http://resources.schoolscience.co.uk/ICI/14-16/catalysis/catsch8pg3.html Experiment 3 Osmosis http://www.markedbyteachers.com/gcse/science/biology-osmosis-1.html http://homesicks.info/2019/08/27/the-effect-of-solute-concentration-onosmosis-in-potatoes-_m0/ https://www.biologyjunction.com/lab_1_osmosis.htm https://scientificat.wordpress.com/2015/08/14/osmosis-and-diffusionpotato-cores/ https://apbizzle.weebly.com/blog/osmosis-and-diffusion-3-part-lab https://www.biologyjunction.com/ap_lab1_osmosis_sample4.htm Appendix 2 write up sheet Experimental write up Aim Independent variable Dependant variable Controlled variable Control Method summary Rough data table Data table Data table with average calculation Graph Analyse of data Evaluation of experiment Appendix 3 Help Sheet Aim, your aim should contain your dependent and independent variables and also state what the experiment hopes to prove Your variables are important to know when writing your report You must summarise the method into a simple paragraph Make a rough table on what data you will collect (your teacher will look at this before you start your experiment. Make sure heading are all correct and detailed Make a need table after collecting results Make a neat table that has and extra Colum for averages You must be able to say in relation to your aim what your experiment has shown You must identify a factor that had an effect of the reliability of your experiment, You must then explain: — What you did to minimise the effect of this factor Or — What you could have done to minimise the effect of this factor Or — How you know this factor had a significant effect
