BIOL 1406 – Biology for Science Majors Dr. Moore Chapter 10: Photosynthesis Feeding the Biosphere: What is photosynthesis? Autotrophs – o Photoautotrophs – Types of photoautotrophs – Heterotrophs: Consumers Types of organisms that are heterotrophic – Relationship of solar energy to fossil fuels – Light Energy Chemical Energy: Endosymbiosis – o Chloroplasts & photosynthetic bacteria – Light: Electromagnetic energy/radiation – o Light travels in rhythmic ____________ o Wavelength – What is measured – What does this determine – Electromagnetic spectrum – Visible light – Photons – Photosynthetic Pigments: Pigments are _____________ that absorb __________________ Different pigments absorb different _________________; others are reflected or transmitted Only ___________light is useful for photosynthesis Leaves appear green because chlorophyll _________________________ green light Chlorophyll a – o Most abundant in – o Absorbs – o Reflects – Accessory pigments – o What is the function of accessory pigments? o Chlorophyll b – o Absorbs – Reflects – Carotenoids – Reflect __________ wavelengths of light Appear – Also act as __________________ - protect plants from free radicals Spectrophotometer: Measures – Excitation of a Chlorophyll Molecule: Structural Components: Leaves – o o Stomata CO2 O2 Mesophyll – Roots – Chloroplasts: Stroma – Granum – Thylakoid – Thylakoid space – Photosystems – Reaction Centers – Reactions of Photosynthesis: Write the reaction for photosynthesis: Tracking Atoms: H2O is split Electrons of ____ will be incorporated into __________ molecules _____ is released as a by-product Stages of Photosynthesis: Light Reactions – o Split _________ and release ________ o Reduce ________ to _________ o This is a transfer of ______________ Generate ATP by ________________________ Calvin Cycle – o Occurs in the ____________ o Forms _____________ from _________ using ATP and ____________ o Carbon fixation – Photosystems: Reaction-center complex – Light-harvesting complexes – Primary electron acceptor – Two types – o Photosystem II (PS II) – Best absorbs wavelength of _________ nm Reaction center is called ________ o Photosystem I (PS I) – Best absorbs wavelength of _________ nm Reaction center chlorophyll is called _________ Linear Electron Flow: Primary pathway – A ____________ hits a pigment & energy is passed until it excites __________ o Excited __________ is transferred from _______ to the ________________ H20 is split transfers ______ to ________ and releasing ________ as a by-product Each electron “falls” down an ____________________ from primary electron acceptor of PS II PS I o Energy releases drives creation of __________________ across thylakoid membrane o Diffusion of _________ across the membrane drives _________ synthesis PS I – light energy excites ________ electron is transferred to ___________________ o Where did this electron come from? Primary electron acceptor passes electron to ferredoxin (Fd) and then to ________ Light Dependent Reactions – Linear Electron Flow: Cyclic Electron Flow: Uses only Photosystem _____ o Produces _________ o Does not release ________ Generates surplus ________ to satisfy Calvin Cycle Evolutionary implications?? Chemiosmosis: Used by both mitochondria and chloroplasts to ____________________ o Mitochondria – o Chloroplasts – Thylakoid Membrane: Calvin Cycle: Three phases – o Carbon fixation – o Reduction – o Regeneration – Carbon Fixation: CO2 molecule combines with _______ acceptor __________ Makes a 6C compound that splits into two molecules of _________ (3 carbon) Reaction is catalyzed by ____________ Reduction: ATP & NADPH are used to convert ________ into 3C sugar ______ Why is this called reduction? Regeneration: Some _____ molecules go on to make __________ (6 carbon) Others are recycled to regenerate __________ Regeneration requires ________ For each three ______ molecules that enter the cycle, one molecule of ______ is formed Calvin Cycle: How many turns of the cycle are required to make one G3P molecule if a single CO2 molecule can enter at a time? How many ATP are used during the Calvin Cycle? How many NADPH are converted to NADP+ during fixation? To make a single glucose molecule in six turns of the cycle it would require – o _____ CO2 molecules o _____ ATP o _____ NADPH Overview of Photosynthesis: Photorespiration: Energy conversion of photosynthesis is about _____% efficient Further inefficiency can be caused by ______________ o What happens in photorespiration? In photorespiration, rubisco adds _____ instead of CO2 o Produces a ____ carbon compound o Consumes ______ and releases _____ without producing ______ or ________ Why is it likely that photorespiration is an evolutionary relic? In which types of environment might photorespiration be a problem? C3 Plants: Named for _____________ How do they limit photorespiration? What types of problems could this cause in certain environments? Adaptation: Dehydration is a challenge in some environments, how would this occur? Why might a plant close their stomata? What conditions favor photorespiration? C4 Plants: How do these types of plants minimize the cost of photorespiration? Require _____________ to incorporate ____ into four carbon compounds in ___________ cells What happens to the four carbon compounds? How does this limit photorespiration? How do C4 plants separate the reactions of photosynthesis? CAM Plants: In which type of environment do we typically find CAM plants? How do CAM plants separate the reactions of photosynthesis?