THE SUPPORTIVE CONNECTIVE TISSUE AND THE SKELETAL SYSTEM Lab 3 Eman Al-Qattan 1 Study the skeletal system by understanding its major tissue components namely cartilage and bone. OBJECTIVES: Study the structure of three different types of cartilages and bone through prepared slides. Identify the different types of bone joints with examples. Study the major bones of the human skeleton. Compare the features of human pelvis for male and female. Eman Al-Qattan 2 TISSUES OF THE SKELETAL SYSTEM Cartilage ❑ Cartilage is a semi-rigid form of connective tissue, that provides a supportive framework for various structures. Eman Al-Qattan Bone ❑ It is the most rigid form of connective tissue, with deposits of mineral salts and collagen within the matrix. 3 1. Hyaline cartilage ✓ A clear glass like appearance. ✓ Mature cartilage cells are called chondrocytes. TYPES OF CARTILAGE: ✓ Chondrocytes are embedded in matrix spaces known as lacunae. ✓ At the periphery, there is a zone of condensed connective tissue called perichondrium ✓ Cells are separated by a large mass of cartilage matrix known as Chondrin ✓ Examples: nasal septum, larynx, tracheal rings and most articular surfaces. Eman Al-Qattan 4 Perichondrium Chondrin (Matrix) Lacuna Eman Al-Qattan Chondrocyte Capsule 5 2. Elastic cartilage ✓ It contains an abundant network of fine elastic fibers. ✓ Fibers are stained black and is especially dense in the immediate vicinity of the chondrocytes. TYPES OF CARTILAGE: ✓ Elastic cartilage has a yellowish color when fresh due to the presence of elastin in the elastic fibers. ✓ The chondrocytes are large within the interior and tend to become smaller towards the perichondrium. ✓ Elastic cartilage is opaque and more flexible than hyaline cartilage and is not prone to degenerative calcification. ✓ Examples : epiglottis, external auditory canal, external ear and some of the laryngeal cartilages. Eman Al-Qattan 6 Lacuna Eman Al-Qattan 7 3. Fibro Cartilage TYPES OF CARTILAGE: ✓ The chondrocytes are present either singly or in small groups and are very often arranged in long columns between dense collagen layers. ✓ There is little ground substance, and the matrix contains a great number of highly interwoven collagenous fibers. ✓ Examples : found in attachment of certain ligaments to bones, in the pubic symphysis and in the intervertebral discs. Eman Al-Qattan 8 Fibro cartilage in the intervertebral disc. Eman Al-Qattan Chondrocyte Collagen Fibers Lacuna Matrix 9 ❖ It forms a complete system of supporting tissue, namely the skeleton. ❖It is the most rigid form of connective tissue with deposits of mineral salts and collagen within the matrix. THE BONE ❖Bone cells are called osteocytes, which lies within a lacunae. ❖They are arranged in concentric circles (osteons) around the osteon canal interconnected by canaliculi. ❖Bone has a good blood supply, enabling rapid recovery after an injury ✓ Examples: T.S. of Compact bone and L.S. of Compact bone Eman Al-Qattan 10 Eman Al-Qattan 11 T.S. OF COMPACT BONE ✓ The matrix is organized into numerous circular units called osteon (Haversian systems) ✓ In each osteon, calcified matrix is arranged in multiple concentric layers, the lamellae surrounding a central canal known as the Haversian canal ✓ This canal contains blood vessels, nerves and lymph vessels. Eman Al-Qattan 12 Osteocyte Haversian System Haversian Canal Eman Al-Qattan Bone lamellae 13 ✓ Bone cells called osteocytes appear to be between the hard layers of the lamellae in small spaces known as lacunae. T.S. OF COMPACT BONE ✓ Osteocytes are also arranged in concentric rings within the lamellae ✓ Tiny canals, called canaliculi connect the lamellae with one another and with the central canal. ✓ Canaliculi contains fine cytoplasmic extensions of osteocytes. Eman Al-Qattan 14 Osteocytes Eman Al-Qattan 15 L.S. OF COMPACT BONE ✓ In the L.S. of compact bone, Osteon appear to be parallel to the Haversian canal columns. ✓ The central canals are in longitudinal planes interconnected with one another by a certain oblique connection called Volkmann’s canals. Eman Al-Qattan 16 Haversian Canal Osteocytes Volkmann’s Canal Eman Al-Qattan 17 1. Synovial Joints This type of joint is characterized by free and extensive movement of the bone at the articular surfaces. The articular surfaces at the end of the bones are covered by hyaline cartilage. TYPES OF JOINTS: These surfaces are maintained in close contact by a fibrous capsule and ligaments. On the inner surface of this capsule is a specialized connective tissue layer called synovial membrane. Synovial membrane secretes the synovial fluid which lubricates the articular surfaces. Examples of these joints are found between long bones such as at the knee joint, elbow joint etc. Eman Al-Qattan 18 Eman Al-Qattan 19 TYPES OF JOINTS: 2. Fibrous Joint These joints are found between the cranial bones (sutures) and are immovable. Eman Al-Qattan 20 3. Cartilaginous joints: ✓ These are found between the vertebrae, and are slightly movable. TYPES OF JOINTS: Eman Al-Qattan ✓ The two hip bones are also slightly movable because they are ventrally joined by cartilage and respond to pregnancy. 21 BONES OF THE HUMAN SKELETON: Eman Al-Qattan 22 ➢ The entire male hipbones form a narrow structure than a female hipbones STRUCTURAL DIFFERENCES BETWEEN THE MALE AND FEMALE HIPBONES : ➢ A man’s pelvis is shaped like a funnel, but a woman’s pelvis has a broader, shallow shape, more like a basin. ➢ The brim of pelvis is normally much wider in the female than in the male. ➢ The angle at the front of the female pelvis where the 2 pubic bones join is wider than it is in the male. Eman Al-Qattan 23 Eman Al-Qattan 24