LABORATORY
NANOTECHNOLOGY FOR DRUG DELIVERY SYSTEM
Lecturer: Vòng Bính Long PhD.
TA: Đoàn Đức Thế Anh
Ho Chi Minh, October 2021
Laboratory contents
Chapter 1: Preparation & characterization methods for the
synthesis of nanoparticles
Chapter 2: Evaluation of drug loading capacity and release
Chapter 3: Bioactivities evaluation by in vitro assays
Chapter 4: Pharmacokinetics and therapeutic evaluation by in
vivo assays
1
Chapter 2: Evaluation of drug loading
capacity and release
DRUG ENCAPSULATION AND
RELEASE OF DRUG@NPs
3
CONTENTS
•
Drug encapsulation – Drug release
•
Preparation
•
Calculation EE%, LC% and release
•
Data analyzation
4
DRUG ENCAPSULATION
Nanoencapsulation
increase
Stability of drugs/proteins
Protect drugs from degradation
Target the drug to the site of action
Controlled release
5
DRUG ENCAPSULATION
The self-assembly of siRNP. The model is based on Vong et. al., (3) model.
6
DRUG RELEASE
Side-by-side diffusion cells with artificial or biological membrane
Ultracentrifugation
Methods to study the
in vitro release
Ultrafiltration
Dialysis bag diffusion technique
7
DRUG RELEASE
8
PREPARATION – Materials & Equipment
Materials
Role
Chemical formula
Equipment
Falcon
Eppendorf
Micropipette
Micropipette tips
Magnetic stirrer
9
PREPARATION – Curcumin@siRNP
10
DRUG ENCAPSULATION – EE% & LC%
11
DRUG RELEASE – Dialysis method
12
UV-VIS spectrophotometer – calculate
drug concentration
13
Check list
14
Curcumin@RNP synthesis
15
DATA ANALYZATION – EE% & LC%
16
DATA ANALYZATION – Drug release
17
Summary
18