A Hydroponic Co-cultivation System for
Simultaneous and Systematic Analysis of
Plant/Microbe Molecular Interactions
and Signaling
presented by: Yaser Saleh Baamer
supervisor: D.Mahmoud Alhosin
Introduction
The role of plant associated microbes in different plant life
stages is crucial, they influence biogeochemical cycling, plant
growth and health, and many more. Microorganisms interact
with plants directly through plant cell wall contact, and
indirectly via chemical secretion and signaling.
Chemical secretion from roots attracts certain
microorganisms that find perfect condition to flourish in the
rhizosphere. This species-specific attraction either benefit or
detriment the plant host.
Cont…
Arabidopsis thaliana-Agrobacterium tumefaciens is the
model applied to study molecular interaction. Initial stages
of is less well understood, due to the limitation of
conventional approachs. Such as cell suspension which lack
of plant morphology. And artificially wounding plant
method that significally different from pathogen infection in
nature. In addition to supplementation with synthetic
chemicals to induce plant host response or pathogen
virulence.
Cont…
To over these challenges, hydroponic co-cultivation system
that allow for natural cell signaling homeostasis during
plant-microb interaction. Giving us the chance to analyse
plant-microb molecular interactions and signaling in
simultaneous and systematic manner.
Method
Seeds wear sterilized and grow in sterilized system. Then,
after 3 days the system inuclated A.tumefaciens (1x10^7
cell\ml). The attachment of microbe to the root visualized
with fluorescent microscope. Bacterial and plant transcript
analysis, sectrome profiling for compound detection via (ESITOF-MS) wear also done.
Results
Growth in the hydroponic cocultivation system
Cont…
Microscopic visualization of Agrobacterium attachment to plant roots
Cont…
Activation of Agrobacterium virulence transcripts
Cont…
Expression of plant transcripts
Cont…
Alterations to host secretion profiles
Discussion
In this syudy we wear able to mimic natural environment,
and to evaluate exactly gene activity under such condition.
We suggest that future dtudies on high expressed genes and
chemicals is needed