Quantitative Genetics and Biometry (PBG-609) Course Instructor: Dr. Umara Sahar Rana Email Address: umarapbg@uaf.edu.pk Semester : Winter 2021-22 Course Code: PBG-609 Course Title: Quantitative Genetics and Biometry Course Credits: 4(3-1) Course description: This course will provide an understanding of: Quantitative genetics and biometry Introduction of different models of genetic analysis Different statistical analysis used in genetic studies Methods of collection, classification and analysis Use of biometrical approaches in Plant Breeding Drawing inferences from collected data Testing hypothesis Interpretation of the results Use of Excel and statistical software for the data analysis Instructional type: The teaching methods for this course will include lectures with ppt presentation, class discussions and participatory approach, assignments, field visits and exercises. Justification: In biological system, it is very difficult to draw any concrete conclusion about any happening, as there is much difference among the individuals. Such as in the same plant, the leaves are all different; again in a field, all the same kind of plants are not identical; in the same place, the same crop does not respond equally in different pots. So, it is very difficult to reach at a definite and reliable conclusion unless we take the help of biometrical approaches for the data analysis. In case of Plant Breeding and Genetics, the application of biometry has an immense importance, as the statistical analysis only helps to conclude about a population of plant such as: a) How the character which is acquired by the population is distributed, how the character is being inherited etc. b) Whether two characters in a population are correlated or not, if correlated how much they are correlated? c) In case of plant breeding whether Mendelian Genetics is being followed or not, can be tested by Chi-square test. d) The application of fertilizer or irrigation or any kind of cultural practice can be effectively justified by application of test of significance. e) The laws of probability are of importance in genetics for forecasting the chance of obtaining certain result from a cross and elucidating the operation of genetic principle. f) The effects of two or more different fertilizer application on the same field can be analyzed by analysis of variance. g) Study of alleles of genes in population and the factors which maintain or change the frequencies of particular genotype in population can be studied. Theory: This course will provide an overview of introduction to quantitative genetics and biometry. Genetic and somatic analysis. Sources of variation: genic, plasmatic, and interaction of genotype and environment. Introduction to different models for genetic analysis. Principles of scaling and scaling tests. Components of means and variation. Detection of additive-dominance components and non-allelic interaction. Introduction to stability analysis. Biplot analysis for G × E interaction studies. Introduction to different mating design and their application in plant breeding. Heritability: factors affecting magnitude of heritability, and its significance in plant breeding. Estimation of heterosis and interpretation. Correlation and regression analysis. Practical: Collection and tabulation of data on different parameters in field experiments,. Methods of comparisons of means. Estimation of components of means and variances using weighted least square analysis. Estimation of heterosis and heritability. Computation of simple correlation coefficient and its tests of significance. Calculation for combining ability effects Suggested Readings 1. Ali, Z. 2010. Analyzing and Understanding Genetic Problems: Classical and conventional approach. VDM, Germany. 2. Backer, W.A. 1992. Manual of Quantitative Genetics. (5th ed.). Academic Enterprises, Pullman, USA. 3. Kang, M.S. and M. Kang (Eds). 2003. Handbook of Formulas and Software for Plant Geneticists and Breeders. Harworth Press Inc, LA, USA. 4. Kearsey, M.J., and H.S. Pooni. 1996. The Genetic Analysis of Quantitative Traits. Chapman & Hall , London, UK. 5. Mather, K., and J.L. Jinks. 1982. Biometrical Genetics. (3rd ed.). Chapman and Hall Ltd. London, UK. 6. Saville, D.J. and J.S. Rowarth. 2008. Statistical measures, hypotheses, and tests in applied research. J. Nat. Resour. Life Sci., 37:74-82. 7. Singh, P. 2000. Biometrical Techniques in Plant Breeding. (2nd ed.). Kalyani Publishers, New Delhi, India. 8. Singh, R.K. and P.K. Singh. 1999. Biometrical Methods in Quantitative Genetic Analysis. Kalyani Publishers, New Delhi, India.