USER MANUAL
.
INTEGRATED PLATFORM.
FOR PRECLINICAL.
PULMONARY RESEARCH.
WELCOME TO THE FLEXIVENT FX
Congratulations, and thank you for selecting the flexiVent! The flexiVent FX combines the most
accurate, detailed and reproducible measurements of respiratory mechanics with flexible and
efficient experimentation management.
The flexiVent FX operates with flexiWare software in a system designed for
mechanical ventilation and life support
execution of measurement manoeuvres including the Forced Oscillation Technique
(FOT)
management and storage of acquired raw data
data analysis & model fitting
visualization, storage and export of results, and
maintenance of experimental records in electronic format.
This user manual contains important information and should be studied in detail before
operating the flexiVent FX. However, this manual is not intended to be a complete guide of the
flexiWare software. For detailed information about using and configuring the flexiWare
software, please refer to the flexiWare user manual.
The information contained in this manual is believed to be accurate at the time of print.
However, SCIREQ Scientific Respiratory Equipment Inc. does not offer any warranties, express
or implied, for the content of the manual and the use of all related products. The license and
operating conditions apply.
SCIREQ Scientific Respiratory Equipment Inc.
6600 rue St-Urbain, Suite 300
Montreal, Quebec, Canada H2S 3G8
P. 514-286-1429
P. 877-5-SCIREQ (toll-free in Canada and USA; 877-572-4737)
F. 514-286-1627
www.scireq.com
flexiVent User Manual version 6.2.
(Document No: FV-FXUM)
Revised July 31, 2014
© SCIREQ Scientific Respiratory Equipment Inc., 1997–2014.
All rights reserved. This manual may not be reproduced or duplicated in print or any other media, including
electronic storage and transmission, without prior written permission from SCIREQ Scientific Respiratory
Equipment, Inc.
Aerogen and Aeroneb are registered trademarks of Aerogen, Inc., Galway, Ireland.
iv
SAFETY CONSIDERATIONS
WARNINGS
The flexiVent is not intended for use with human subjects.
If you use anaesthetic gas and/or aerosols, take precautions to ensure that the expired
gas/aerosol does not escape into the room air.
Ensure proper evacuation and control of potentially harmful substances being introduced
into the system to protect the user(s).
Disengage the motor lock before connecting a power source.
Do not attempt to open the base unit cover while the instrument is running; there is a
danger of moving parts.
When carrying or moving the flexiVent, always ensure that the device is kept horizontal and
supported by two hands underneath the unit.
The power cord used should comply with local standards.
Use only the power supply provided by SCIREQ with the flexiVent system.
v
Only gases may be introduced through the air intake (e.g. room air, non-flammable mixed
gas, inhaled anesthesia). Introduction of other substances through the air/gas intake, e.g.
particulates or aerosol, may damage the module’s piston.
Always unscrew the piston rod before attempting to remove the module from the base unit.
Handle the module carefully. Avoid straining or bending the piston rod.
Clean the expiratory valve only. Do not attempt to clean the refill or inspiratory valves.
Do not attempt to clean or dry the expiratory line while an FX adapter is in place in the
module. Also, do not attempt to clean or dry the expiratory line while outside of an
experimentation session. Attempting to clean or dry the expiratory line with the valve close
will damage the module.
Do not use abrasive or corrosive detergents or mechanical cleaning aids.
Do not dismantle the module or base unit to attempt to clean it.
Do not use abrasive or sharp tools to clean the nebulizer unit.
Do not autoclave the Aeroneb controller or cables.
Do not immerse the Aeroneb controller or cables in water.
vi
Avoid removing the nebulizer from the mount while a subject is attached. If it is necessary
(e.g. for cleaning), do so quickly and immediately replace the nebulizer or use a cap to close
the ventilator circuit.
When you are using and handling the flexiVent, always observe all of the cautionary notes
included in this manual. SCIREQ is not liable for damage or injury resulting from misuse of this
product.
LIST OF SYMBOLS
The following table describes the electrical and safety symbols that may be displayed on the
flexiVent FX. The safety symbols may also appear throughout this manual and other product
support documents.
SYMBOL DESCRIPTION
Direct current
Alternating current
Caution, risk of electric shock
Caution; closely observe all cautionary statements to prevent
equipment damage and ensure operator safety
Power button
Ethernet network cable connection port
vii
SYMBOL DESCRIPTION
Auxiliary channels
Synchronization port (in)
Synchronization port (out)
Power supply connection
Intake/Inspiratory line
Exhaust/Expiratory line
Inappropriate for disposal in general waste
Aluminum components; please recycle as appropriate
Internal magnet
viii
TABLE OF CONTENTS
1
Introduction ....................................................................................................................................................................11
1.1
Intended Use ........................................................................................................................................................... 11
1.2
UnitWise Platform & flexiWare.................................................................................................................... 12
1.3
flexiVent FX System Overview ..................................................................................................................... 16
1.4
Theory of Operation ............................................................................................................................................ 17
2
Set up & Installation ................................................................................................................................................. 25
2.1
Selecting a Location ...........................................................................................................................................25
2.2
Preparing for Installation ...........................................................................................................................25
2.3
Placing the System ....................................................................................................................................... 28
2.4
Connecting the Components ................................................................................................................... 28
2.5
Opening the flexiVent protective cover .............................................................................................33
2.6
Working with an Alternate Module....................................................................................................... 34
2.7
Configuring flexiWare................................................................................................................................. 38
3
Getting Started ......................................................................................................................................................... 39
4
Accessories ................................................................................................................................................................. 50
4.1
UNIT Transducers ............................................................................................................................................... 50
ix
4.2
Nebulizer............................................................................................................................................................ 54
4.3
Calibration Accessories ..............................................................................................................................57
4.4
Hardware Extension for Negative Pressure Forced Expirations (NPFE).......................... 58
4.5
Multi-subject Extension (MSX) ..............................................................................................................60
5
Cleaning, Decontamination and Maintenance ............................................................................................. 61
5.1
Cylinder ..................................................................................................................................................................... 61
5.2
Cleaning flow pathways and Valves ..................................................................................................... 62
5.3
FX Adapter and Y-tubing ...........................................................................................................................66
5.4
External Surfaces ..........................................................................................................................................66
5.5
Aeroneb Nebulizer ........................................................................................................................................ 67
5.6
Annual Cleaning and Maintenance ........................................................................................................68
5.7
Repackaging, Transport and Storage..................................................................................................69
5.8
Replacement Components ....................................................................................................................... 74
Appendix A: Technical Specifications .........................................................................................................................76
x
11
1 INTRODUCTION
The flexiVent FX is a UnitWise instrument most often used for assessment of respiratory
mechanics in pre-clinical research. Your system is customized to your research application and
may be enhanced as new products are released.
1.1
INTENDED USE
At its core, the flexiVent is a computer-controlled piston ventilator that allows you control over
ventilation parameters. To perform an assessment of respiratory mechanics, ventilation is
momentarily suspended and a pre-defined volume waveform is applied by the piston. The data
collected during the manoeuvre are analyzed by the software, which reports measurements of
mechanics. The computer control of the piston and precision with which data are collected
generates accurate, reproducible measurements of respiratory mechanics.
The flexiVent FX is designed for full mechanics assessments in subjects weighing
approximately 8 g to 1 kg. It may be used for ventilation and mechanics measurements from
small amplitude manoeuvres in subjects weighing approximately 8 g to 5 kg. Additional details
are available in Table A-2.
If you intend to use the flexiVent outside typical operating conditions explained in this user
manual, please contact SCIREQ Technical Support for additional instructions and
documentation.
The flexiVent is not intended for use with human subjects.
12
1.2
UNITWISE PLATFORM & FLEXIWARE
The flexiVent FX is built on the second generation UnitWise platform, a fully digital, data
acquisition and control architecture designed to be operated by flexiWare software. For
additional details on flexiWare refer to the flexiWare user manual.
FIGURE 1-1: UNITWISE PLATFORM
The flexiWare/UnitWise platform consists of a variety of different components that are
optimized for their individual tasks and communicate digitally with each other, as illustrated in
Figure 1-1. This section provides an overview of the different platform components to permit
users a better understanding of the interplay between the different system components.
13
1.2.1
WORKSTATIONS
The UnitWise platform uses standard Ethernet connectivity as the backbone to connect the
high-level components of a UnitWise data acquisition and experimentation environment. The
list of high-level components includes the individual UnitWise instruments (see section 1.2.2),
experimentation and review workstations (both running flexiWare), and the database server on
which data may be stored in a central location.
In the simplest scenario, a single stand-alone computer can serve as experimentation
workstation, review workstation and database server. On the other hand, the
UnitWise/flexiWare architecture permits distributed multi-client environments where data may
be simultaneously collected from several parallel workstations into a central database, allowing
study directors to observe the evolution of the experiment in real time from the comfort of
their office.
1.2.2
INSTRUMENTS & SYSTEMS
In UnitWise terminology, “instruments” are compact laboratory instrumentation devices that
possess both an Ethernet connection and several ports for Universal Intelligent Transducers
(UNITs). Recent UnitWise instruments also feature a display and local user interface on the
device. Instruments serve as the central building blocks of UnitWise data acquisition systems
and perform the large majority of all real-time data acquisition, control and automation
functions.
In UnitWise terminology, “systems” comprise one or more instruments as well as a variety of
UNITs selected according to the application at hand, connected together in such a way that the
specific data acquisition and control needs of this application are met. Every UnitWise system
must contain one primary instrument, and may contain one or more secondary instruments. The
secondary instruments are controlled by the primary instrument, permitting phase-locked data
acquisition systems with a virtually unlimited number of channels.
14
FIGURE 1-2: UNIVERSAL INTELLIGENT TRANSDUCER (UNIT) ARCHITECTURE
1.2.3
PORTS, UNITS & CHANNELS
UnitWise instruments typically possess eight ports, each of which may be occupied by one
Universal Intelligent Transducer (UNIT) without further adapters, amplifiers or pods, so that any
UNIT can fit into and be recognized by any instrument port (see Figure 1-2).
Every UNIT contains between one and four sensor elements or other input/output channels,
each permitting the measurement or modification of a physical property. To optimize the signalto-noise ratios, all input signals are digitized right in the UNIT. A small micro-controller (μC)
15
contained in every UNIT scales and linearizes the signals and performs self-tests and local
control functions. The controller also retains calibration and identification details that are
communicated digitally to the instrument and the host software to log the system configuration
and assert its integrity.
A common example for a simple UNIT is shown in UNIT A in Figure 1-2. This UNIT has a single
analog input channel to measure a signal from a single sensing element and communicate it to
the instrument and the software. A precision differential pressure transducer such as model
UT-DPD-02 is an example of this type of UNIT.
UNIT B in Figure 1-2 provides an example of local low-level servo control. This UNIT combines an
analog input channel with an embedded actuator that has the ability to manipulate the attribute
being measured in order to simultaneously collect the actual measurement and push it towards
a desired value as provided by the instrument and/or the software. An example for this type of
UNIT would be a temperature-controlled water bath.
UNIT C in Figure 1-2 provides a further example of the platform’s flexibility as it shows 3 analog
input channels communicating measurements to the software while a fourth digital output
channel is used to allow the software to switch or send synchronization signals to outside
instruments.
1.2.4
INTEGRATED SYSTEMS
In UnitWise terminology, some SCIREQ products are referred to as “integrated systems” since
they contain both an instrument and one or more UNITs in a common package. For example, a
flexiVent FX has all features of a UnitWise instrument (Ethernet, ports and embedded display)
but also possesses several internal ports to measure and control volume displacement,
pressures and valve states. Integrated systems such as the flexiVent typically feature a limited
number of external (auxiliary) ports for accessories, and their port count can be further
extended by adding a secondary instrument (see section 1.2.2).
Some integrated systems offer functionality that goes above and beyond the capabilities of a
standard UnitWise data acquisition system (e.g. mechanical ventilation for the flexiVent, pump
profiles for the inExpose). When working with an integrated system such as the inExpose or the
16
flexiVent, menus and commands are automatically configured in the software such that you
have complete access to your instrument’s functionality. The instrument-specific configuration
is controlled by flexiWare licensing, SDMs and template mechanisms. It does not require any
additional steps or configuration settings during operation. All commands necessary for your
application are available for your use, following setup and installation.
1.3
FLEXIVENT FX SYSTEM OVERVIEW
The flexiVent FX is composed of two main parts: the base unit and a module. The base unit
includes the following:
a computer-controlled linear actuator;
an optical position sensor;
a processing platform (controller);
a base plate with a protective enclosure ;
a module connection platform; and
an embedded user interface.
A module is comprised of the following:
a precision piston-cylinder set;
three computer-controlled valves;
pressure transducers;
a micro-processor; and
air flow pathways and connections.
For additional details, see section 1.2.
17
1.4
THEORY OF OPERATION
As is mentioned in section 1.1, the flexiVent is a computer controlled piston ventilator. The
computer control afforded by flexiWare software makes the flexiVent versatile because you
control the parameters of the ventilation and measurement manoeuvres that it executes. Its
design adds to its versatility in part because it may be used with a range of subject sizes, but
also because it allows for the integration of a variety of accessories and hardware extensions,
which includes both SCIREQ products and third-party products.
1.4.1
RESPIRATORY MECHANICS
Generally speaking, mechanics is the relationship of objects and the forces that act upon them.
Respiratory mechanics is a way to quantify the airway constriction and stiffness of the lungs
through relationships between pressure, volume and flow using mathematical models.
1.4.2
FORCED OSCILLATION TECHNIQUE
Measurement manoeuvres executed by the flexiVent are referred to as perturbations. During a
perturbation, an iso-volume ventilator compartment is established when the valves within the
module close to the outside environment. The ventilator compartment consists of the subjects
respiratory system, the cylinder, pathways outside the module (e.g. Y-tubing) and pathways
within the module (e.g. from the cylinder to the Y-tubing). Once the valves are closed, the
perturbation, or Forced Oscillation, is applied to the ventilator compartment through movement
of the piston (see Figure 1-3). The signals generated during the perturbation are used to
calculate parameters of respiratory mechanics that help to quantify acute and/or chronic
disease-related changes to the lungs.
18
FIGURE 1-3: VALVE CONFIGURATION DURING A PERTURBATION
There are six families of perturbations. A description of each follows. The properties (e.g.
duration, maximum pressure) for the perturbations are established in the flexiWare software.
For detailed information about perturbation properties, refer to the flexiWare user manual.
TABLE 1-1: STANDARD FLEXIVENT PERTURBATIONS
NAME
DESCRIPTION
Deep
Inflation
Deep inflation of the subject's
lungs to a pressure of 30 cmH20
(or other user-specified value)
followed by a breath hold of
typically a few seconds.
SAMPLE
19
NAME
DESCRIPTION
SnapShot
Single frequency, sinusoidal
forced oscillation waveform. The
oscillation frequency is typically
matched to the subject's
respiratory rate (e.g. "SnapShot150" for mice breathing at 150
br/min).
Broadband (multi-frequency)
forced oscillation waveforms,
Primewave
typically denoted by duration (e.g.
"Prime-8" lasts 8 seconds).
PV Loop
Slow stepwise or continuous
(ramp) inflation to TLC (or other
user-specified value) and deflation
back to FRC, controlling either
volume or pressure.
SAMPLE
20
NAME
DESCRIPTION
NPFE
Deep inflation followed by rapid
switch to negative pressure
reservoir (hardware extension
required).
Imaging
Pressure-controlled breath-hold,
with or without a preceding deep
inflation with synchronized trigger
signals for imaging modalities such
as Micro-CT or microscopes
(imaging system not supplied by
SCIREQ, auxiliary hardware
required).
1.4.3
SAMPLE
INDIRECT MEASUREMENT
The flexiVent uses the indirect measurement technique to assess respiratory mechanics. In
animal subjects, especially those that do not generate large tidal flows, precise flow
measurement can be quite difficult. Flow measurement difficulty is compounded by signal
losses due to gas compression, so use of flow measurements to calculate mechanics is
technically challenging. The flexiVent uses measurements of position and pressure, both which
can be measured with great precision and consistency, to calculate mechanics outcomes.
The indirect measurement technique also helps address the technical challenge introduced by
the signal effects of gas compression and cannula resistance. During the inspiratory portion of
ventilation the pressure in the cylinder is slightly higher than that at the airway opening, and the
airway opening pressure is slightly higher than that within the subject’s trachea. Similarly, the
21
volume of air displaced by the piston is slightly higher than the volume delivered to the subject.
Before any perturbations are run with the subject attached, the ventilator compartment is
characterized for each perturbation. When the subject is attached and a perturbation
subsequently run, the contribution of the subject may be isolated by subtracting that of the
ventilator compartment.
1.4.4
SERVO-CONTROLLED PISTON
The module of the flexiVent FX contains the piston/cylinder set. Movement of the piston is
servo-controlled through a closed feedback-control loop as in Figure 1-2. The piston is driven by
the linear actuator in the base unit and its position is measured by an optical sensor. The
differential pressures in the cylinder and ventilator compartment are measured by transducers
within the module. When the piston moves, it causes a change in the pressures measured by the
transducers. These pressures are measured for the purpose of calculating respiratory
mechanics, but also to provide feedback so the piston’s movement may be adjusted as needed
(e.g. it will move less to generate a given pressure following administration of a
bronchoconstrictor).
1.4.5
CONTROLLING EXPERIMENTAL CONDITIONS
Section 1.4.3 discusses a technical challenge in pre-clinical respiratory mechanics. An additional
physiological challenge is addressed by controlling experimental conditions. Mechanics
assessments using other methods may be based on measurements taken breath-by-breath in
spontaneously breathing subjects. As breathing frequency increases, resistance decreases.
Respiratory mechanics also vary with tidal volume. If breath-by-breath measurements are taken
following any variation in the experimental conditions, it is not possible to distinguish
mechanics changes caused by that variation from mechanics changes that may be introduced
due to an increase or decrease in breathing frequency, for example. As discussed in section 1.4.2
the flexiVent takes measurements during perturbations. Perturbation properties are set in the
software, so little to no variation exists between subjects.
22
1.4.5.1
STANDARDIZING VOLUME HISTORY
A subject’s volume history affects mechanics measurements. Ventilation parameters (e.g. tidal
volume, I/E ratio) are set in the software to ensure that all subjects in a study are ventilated in a
similar manner. In addition, the Deep Inflation perturbation is commonly used to re-set or
standardize volume history. This is especially true in dose response studies (in which the Deep
Inflation may be used to help subjects return to baseline before subsequent doses are
administered) and imaging studies (in which the Deep Inflation is used to ensure consistent
inflation prior to image acquisition).
1.4.6
INTERCHANGEABLE MODULES
As mentioned in section 1.3, the flexiVent FX piston/cylinder set and valves that create the
ventilator compartment are mounted on the portion of the system called the module. There are
five modules currently available. Each module is designed to minimize its inherent resistance
which permits the highest precision mechanics measurements. The piston/cylinder set size
determines whether or not the system is appropriate for work with a given subject, so the
system can be easily adapted for a wide range of subject sizes. Furthermore, one base unit may
be used with a variety of subjects by incorporating additional modules. Refer to Table A-2 for
module specifications, including estimates for subject weight ranges.
1.4.7
INTEGRATING A NEBULIZER
The Aerogen Aeroneb ultrasonic nebulizer can be integrated with the flexiVent FX in order to
deliver aerosols to the subject. The parameters of aerosol production are set in the software,
which allows for precise control of the aerosol delivery. Specially designed adapters allow tight
integration of the nebulizer to minimize aerosol rainout. The flow pathways within the adapters
are also designed to minimize the addition of volume to the ventilator compartment for
nebulizer integration. Refer to section 4.2 for additional details about the nebulizer. Refer to the
flexiWare user manual for additional details on software settings to control the nebulizer as
well as nebulizer output rate and rainout characterization.
23
1.4.8
APPLYING PEEP
Positive end expiratory pressure, or PEEP, is applied to subjects being mechanically ventilated
to prevent airway closure. One method for applying PEEP is to submerge expiratory tubing into
a column or beaker of water; this method is used in earlier generation flexiVent instruments. To
adjust the PEEP applied using a water column, the level to which the tubing is submerged is
adjusted. In the flexiVent FX, the valves within the module are specifically selected to allow for
computer-controlled PEEP, thereby eliminating the need to use the historical water column
method. The level of PEEP applied may be adjusted in the ventilation pattern dialogue in the
software and may be easily adjusted.
1.4.9
INTEGRATING OTHER ACCESSORIES
The flexiVent FX is highly configurable and may be integrated with other devices, including UNIT
transducers, SCIREQ product extensions geared toward particular measurements (e.g. SCIREQ
NPFE hardware extension), and third party devices (e.g. Micro-CT scanner for gated image
acquisition). The integration may require customized adapters and/or data files for full
functionality. For questions regarding integration with a particular accessory, contact SCIREQ
Technical Support or refer to user instructions provided with your additional hardware. Please
note that only accessories approved and recommended by SCIREQ Technical support should be
used in conjunction with the flexiVent FX.
24
25
2 SET UP & INSTALLATION
The installation sequence described in this section applies to the hardware portion of
configuring a flexiVent FX instrument for use in a UnitWise system. Customized installation
procedures may be required for some application-specific configurations. Please read the set
up and installation instructions for all of your system components before proceeding.
When carrying or moving the flexiVent, always ensure that the device is kept
horizontal and supported by two hands underneath the unit.
2.1
SELECTING A LOCATION
The flexiVent FX requires approximately 2’ x 2’ (60 x 60 cm) of level workspace near the data
acquisition workstation. The flexiVent needs to connect to at least one power outlet (more may
be necessary if your instrument includes certain hardware extensions). Your workstation likely
requires two outlets, one for the monitor and one for the CPU. With these electricity
requirements in mind, select a location for your workstation and flexiVent FX that has at least
three outlets, adequate workspace and that allows all users to easily access all connections
present on the flexiVent FX.
2.2
PREPARING FOR INSTALLATION
When the shipment arrives, carefully remove the flexiVent FX from the boxes and packaging.
These should be kept, if possible, as they may be used for future transportation of the system
(e.g. routine maintenance) or to prevent any damages to the instrument when it is not used for
26
an extended period of time (see section 2.6.1). The flexiVent base unit is shipped with a module
attached. If your instrument includes additional modules, they are shipped in separate boxes.
The following components are part of every flexiVent FX system:
a flexiVent FX base unit;
one or more flexiVent FX modules;
a power supply;
an Ethernet cable;
THE FLEXIVENT AT A GLANCE
(OPPOSITE PAGE)
one or more FX adapters;
1
BASE UNIT
one or more Y-tubing kits;
2
EMBEDDED DISPLAY
one flexiWare installation DVD; and
3
CONTROLLER
flexiWare and flexiVent FX user manuals.
4
MODULE
5
PISTON/CYLINDER SET
6
FX ADAPTER (PICTURED WITH
NEBULIZER AND Y-TUBING ATTACHED)
an Aeroneb controller,
7
AIR/GAS INTAKE
an Aeroneb nebulizer,
8
AIR/GAS EXHAUST
an FX adapter with nebulizer mount, and
9
AUXILIARY PORTS (4)
a rainout characterization kit including a drying tube;
10 SYNC PORT (IN)
The system may also contain the following optional components:
Nebulizer setup, which includes
a pressure manometer;
11 SYNC PORT (OUT)
one or more UNIT transducers
12 ETHERNET PORT
appropriate Allen keys and screwdriver; and
13 POWER CONNECTOR
other product extensions or accessories.
27
1
2
3
7
10
8
11
4
5
12
9
6
FIGURE 2-1: THE FLEXIVENT FX
13
28
2.3
PLACING THE SYSTEM
The flexiVent and all extensions require a dry, flat, stable workspace of 2’ x 2’ x 2’ (60 x 60 x 60
cm). Ensure that the system is stable on the workbench. If the surface is not quite level, use the
adjustable foot located under the front corner below the module to help level it and prevent
unwanted vibrations.
Once the flexiVent is in place, you may disengage the motor lock. To disengage the lock (refer to
Figure 2-2), remove the thumb screw (A) then rotate the motor lock to open position (B). After
disengaging the lock, reattach the thumb screw to the FX base unit (C).
FIGURE 2-2: DISENGAGING THE MOTOR LOCK
2.4
CONNECTING THE COMPONENTS
Please refer to Figure 2-1 for each of the connection locations. Plug in and unplug accessories
only when the base unit is off.
29
2.4.1
POWER
Disengage the motor lock before connecting a power source. Refer to section 2.3 for
details.
The power cord used should comply with local standards.
Use only the power supply provided by SCIREQ with the flexiVent system.
Ensure that the motor lock is disengaged. Connect the power supply to the appropriate location
on the controller module. Plug the power supply into a standard wall outlet using the
appropriate cord.
2.4.2
WORKSTATION OR NETWORK
Connect an Ethernet cable to the Ethernet port on the controller module. Connect the other end
of the cable to a server, data acquisition workstation or network port. The appropriate
connection for the Ethernet cable varies with the complexity of your UnitWise system.
2.4.3
TRANSDUCERS & ACCESSORIES
Connect transducers and accessories (e.g. UNIT Aeroneb adapter cable) to the auxiliary ports
found on the controller module. Selection of the appropriate port is determined by the template
selected for experimentation, and a prompt will appear during the start-up sequence of
experimentation denoting the appropriate configuration. A selection of configurations is
provided in Table 2-1; for configurations including hardware extensions or custom applications,
refer to documentation that accompanies the hardware or information supplied by SCIREQ
Technical Support.
30
TABLE 2-1: SELECTION OF AUXILIARY PORT CONFIGURATIONS
AUX
PORT
AEROSOL
AEROSOL AND
EKG
AEROSOL AND
ALL VITAL SIGNS
DEFAULT
1
none
UNIT Aeroneb
adapter cable
UNIT Aeroneb
adapter cable
UNIT Aeroneb
adapter cable
2
none
none
UNIT EKG
transducer
UNIT EKG
transducer
3
none
none
none
UNIT Temperature
transducer
4
none
none
none
UNIT blood
pressure
transducer
Once the transducer or accessory hardware is connected, its cable may be threaded underneath
the center of the base unit if needed for ease of subject access.
2.4.4
AIR INTAKE & EXHAUST
The intake and exhaust connections are at the rear side of the module. These connections exist
in order to introduce an external gas source (e.g. filtered air or air with specific gas
concentration) or to manage the exhaled gases (e.g. for collection or evacuation).
2.4.4.1
ALTERNATE INTAKE SOURCE
Alternate air sources or inhaled anaesthesia may be used with the flexiVent. It is extremely
important that the intake is not pressurized. In order to install an alternate external air source,
use one of two methods to ensure that no pressure is applied:
1)
Use a very compliant bag and fill it with the desired gas mixture or the anaesthesia gas.
Attach it directly to the intake port using a Quick-Connect fitting. Every time the piston
31
pulls back, it will draw in some of the gas mixture and then deliver it to the subject. Since
the bag is very compliant, it will not pressurize the system.
2) Use a flow-past circuit, which involves putting a T-piece on the intake (refill) port as shown
in Figure 2-3. You will have to supply your own T-piece as every gas anaesthesia machine
has different sized tubing. The T-piece’s single port will be attached to the intake valve
tubing. The anaesthetic gas mixture will then be flowing past the intake valve, and not
directly pressurizing the port. Every time the piston pulls back, it will draw in some of the
gas mixture and then deliver it to the subject. Extra gas can be re-circulated to the
anaesthesia machine or sent to exhaust (e.g. fume hood).
FIGURE 2-3: GAS ANAESTHESIA INTEGRATION
Do not pressurize the air/gas intake or exhaust.
32
Only gases may be introduced through the air intake (e.g. room air, non-flammable mixed
gas, inhaled anesthesia). Introduction of other substances through the air/gas intake, e.g.
particulates or aerosol, may damage the module’s piston.
If you use anaesthetic gas and/or aerosols, take precautions to ensure that the expired
gas/aerosol does not escape into the room air as this can be hazardous for the operator.
2.4.5
SUBJECTS
Before attaching a subject to the flexiVent, proceed through the start-up sequence described in
section 1. Failure to initiate the start-up sequence in advance of connecting the subject may
result in loss of the subject.
Connect subjects to the flexiVent using a cannula or endotracheal tube that is attached to the Ytubing at the front of the module. The Y-tubing must be connected to an FX adapter (with or
without a nebulizer mount, refer to Figure 2-4) which is inserted into the opening on the front of
the module. You will hear a click when the adapter is securely in place.
33
FIGURE 2-4: FX ADAPTER SHOWN WITH NEBULIZER MOUNT AND WITHOUT NEBULIZER MOUNT;
SUBJECTS ARE ATTACHED AT THE END OF THE Y-TUBING.
2.5
OPENING THE FLEXIVENT PROTECTIVE COVER
The flexiVent cover is locked in order to ensure protection from moving parts. Unlocking the
cover should only be necessary in order to replace the module or for maintenance purposes.
Please carefully read all relevant documentation before opening the flexiVent FX cover.
1)
Power off the flexiVent and disconnect it from its power supply.
2) Introduce a pen or other such tool into the small opening as shown in Figure 2-5
34
FIGURE 2-5: COVER LOCK MECHANISM
3) Press gently to unlock the cover.
4) While pressing gently lift the cover to open it.
2.6
WORKING WITH AN ALTERNATE MODULE
As mentioned in section 2.2, the instrument ships with a module attached. If you have ordered an
instrument that contains multiple modules, they will ship in separate packages. If you would like
to work with a module that shipped separately, you must assemble the alternate module,
remove the one that is attached to the base unit, and then install the alternate module on the
base unit.
2.6.1
ASSEMBLING A SEPARATELY SHIPPED MODULE
To assemble the alternate module, follow the module-specific instructions below or refer to the
instruction card that is included within the module’s packaging.
35
2.6.1.1
FX MODULES 1, 2 AND 3
FIGURE 2-6: ASSEMBLY STEPS, FX MODULES 1, 2 AND 3
5) Align the O-ring and the piston/cylinder assembly with the module per Figure 2-6.
6) Insert screws to hold the piston/cylinder in place
This step requires a 7/64” hex/allen wrench.
Screws should be secure, but do not over-tighten them, as this may cause damage.
36
2.6.1.2
FX MODULES 4 AND 5
FIGURE 2-7: ASSEMBLY STEPS, FX MODULES 4 AND 5
1)
Align the O-ring and the piston/cylinder assembly with the module per Figure 2-7.
2) Rotate the piston/cylinder clockwise until it is secure, but do not over-tighten as this may
cause damage.
2.6.2
1)
REMOVING A MODULE FROM THE FX BASE UNIT
Power off the flexiVent and disconnect it from its power supply.
2) Open the base unit protective cover.
37
3) Unscrew the piston rod from the motor arm per Figure 2-8.
FIGURE 2-8: PISTON ROD UNSCREWED FROM THE MOTOR ARM (LEFT)
4) Loosen the module securing screw.
FIGURE 2-9: MODULE SECURING SCREW
5) Lift module upwards.
Be sure to unscrew the piston before attempting to remove the module.
Handle the module carefully.
Take extra precautions to protect piston rod because minor bending could cause it to break
or cease to function properly.
2.6.3
1)
INSTALLING AN ALTERNATE MODULE
Be sure to complete the steps detailed in sections 2.6.1 and 2.6.2 before proceeding.
38
2) Power off the flexiVent and disconnect it from the power supply.
3) Open the base unit protective cover.
4) Lower the module onto the base unit.
The module snaps into place once it is positioned correctly
5) Tighten the module securing screw (see Figure 2-9).
6) Attach the piston rod to the motor arm per Figure 2-10.
FIGURE 2-10: PISTON ROD CONNECTED TO THE MOTOR ARM (RIGHT)
2.7
CONFIGURING FLEXIWARE
The flexiVent FX is operated by flexiWare software. To complete the instrument installation,
please refer to the flexiWare user manual, section 2 (Set Up & Installation). These sections
guide you through the steps necessary to connect the flexiVent to the workstation(s), server or
network desired.
39
3 GETTING STARTED
When you are using and handling the flexiVent FX, always observe all of the cautionary safety
notes included in this manual. SCIREQ is not liable for damage or injury resulting from misuse of
the product.
The following section is designed as a quick guide to using the flexiVent FX, and it notes only
those features in the experimentation session module of flexiWare software that are required
for hardware operation. To make the most of the software functionality (e.g. study definition
and planning and data review and reporting modules), refer to the flexiWare user manual.
STEP-BY-STEP INSTRUCTIONS
An existing connection between the flexiVent FX and flexiWare software is required to proceed
through the following step-by-step guide. If you have not completed the steps detailed in
section 2, go back and review them.
1)
Power on the flexiVent by pressing the power button next to the embedded user interface,
on the front of the instrument.
When the flexiVent is on, its cylinder and embedded display are illuminated.
Do not open the base unit cover while the instrument is running.
2) Start flexiWare by double-clicking the
icon on the desktop. If an icon does not exist, go
to the start menu and select All Programs  SCIREQ flexiWare  flexiWare 7. Once the
software is launched, the Welcome dialogue is displayed.
40
FIGURE 3-1: FLEXIWARE WELCOME DIALOGUE
3) Start an experimentation session by selecting the Experimentation Session button on the
Welcome dialogue, as highlighted in Figure 3-1.
4) Select Default Study in the Study Selection dialogue.
The default study contains two subject groups, Control and Experimental.
Should you prefer to use a study that is customized to your research, refer to the
flexiWare user manual for guidance using the Study Definition & Planning module of
the software.
5) Enter the operator name or initials, experimentation session title and pertinent comments
about the session in the Session Properties dialogue.
6) Select a template from the list of those available. The template you select must match the
hardware configuration you are using. For more information on templates, including the
41
naming convention and a guide for choosing the appropriate template, refer to the
flexiVent-Specific Features section of the flexiWare user manual.
7) Confirm the module that is attached to the flexiVent.
8) Click Subject Management to define one or more subjects that will be used in the current
experimentation session.
To define a subject, assign it a unique identifier and define its weight as shown in
Figure 3-2. Other fields (e.g. strain, gender) are optional.
FIGURE 3-2: SUBJECT PROPERTIES DETAILED IN SUBJECT MANAGEMENT
9) Click Group Membership, and then select the group to which the subject should be added
using the drop-down menu, per Figure 3-3.
42
FIGURE 3-3: GROUP MEMBERSHIP SELECTION OF SUBJECT DETAILS
10) Click Ok to exit subject management.
11) Drag and drop the subject onto the measurement site.
FIGURE 3-4: ASSIGNING A SUBJECT TO THE MEASUREMENT SITE
12) If you have selected a template that includes aerosol delivery (e.g. a default template with
‘AN’ in its name), you will be prompted to prime the nebulizer. The settings for this are preconfigured in the software. Simply ensure that the nebulizer reservoir contains 1-2 mL of
fluid (e.g. saline), remove the nebulizer from its mount on the FX adapter and then click OK.
43
The nebulizer will generate aerosol for a few seconds. Once this is complete place it
securely on the mount of the FX adapter.
13) Proceed to channel calibration
Calibrate the cylinder pressure channel. Most templates require a two point calibration
in which the first point is 0 mmH2O (open to atmosphere) and the second point is
approximately 300 mmH2O (applied using a manometer) to cover the range of
expected pressures. The square to the right of the bar will turn green when a pressure
adequate for calibration is reached.
During Step 4 of the calibration, enter the exact pressure value applied
by the using manometer. For example, if the pressure actually applied was 295 mmH2O,
enter 295 mmH2O; do not retain the default value of 300 mmH2O that is initially
displayed.
FIGURE 3-5: STEP 4 OF CHANNEL CALIBRATION, CYLINDER PRESSURE
Verify the calibration results when they are displayed (see Figure 3-6). Cylinder
pressure channel calibration values should fall within the following ranges:
Max. value: 80.0 cmH2O ± 5 cmH2O
Min. value: -80.0 cmH2O ± 5 cmH2O
44
FIGURE 3-6: RESULTS OF CALIBRATING THE CYLINDER PRESSURE CHANNEL
If your values are not within the expected range, the pressure applied with the
manometer may not have matched the value entered in the calibration dialogue. Note
that if you are using a U-shaped manometer, the amount of pressure applied is equal to
double the change in height on one of the columns. If you used a SCIREQ manometer,
the amount of pressure applied is equal to the level of fluid in the single column. Please
refer to the instructions provided with the SCIREQ manometer for further details. If
you have questions concerning the use of the SCIREQ manometer, contact Technical
Support.
Calibrate the airway opening pressure channel. Again, most templates require a two
point calibration in which the first point is 0 mmH2O (open to atmosphere) and the
second point is approximately 300 mmH2O (applied using a manometer) to cover the
range of expected pressures. Be sure to enter the actual pressure applied in Step 8
(see Figure 3-7).
45
FIGURE 3-7: STEP 8 OF CHANNEL CALIBRATION FOR CYLINDER PRESSURE
Verify the calibration results when they are displayed (see Figure 3-8). Airway opening
pressure channel calibration values should fall within the following ranges:
Max. value: 80.0 cmH2O ± 5 cmH2O
Min. value: -80.0 cmH2O ± 5 cmH2O
FIGURE 3-8: RESULTS OF CALIBRATING THE AIRWAY OPENING PRESSURE CHANNEL
If your values are not within the expected range, please see the note above regarding
proper use of a manometer. Should you have questions, contact SCIREQ Technical
Support.
Characterize your Aeroneb nebulizer to obtain its output rate as well as the delivery
ratio of your configuration. To perform this characterization, an accurate mass balance
46
(0.001g) and Rainout characterization kit will be required. Refer to the Advanced
features section in the flexiWare user manual for further information.
Verify the results when they are displayed. The nebulizer output rate should be greater
than the values listed below for each nebulizer type.
Fine mist (ANP-1100): 0.1 ml/min
Standard mist (ANP-1000):0.3 ml/min
Additional channels may require calibration depending on the template selected.
Please refer to application specific documentation for further instructions.
14) Proceed to dynamic tube calibration
.
This calibration must be performed with a cannula attached to the end of the Y-tubing.
Use the exact cannula that will be used for the experimentation.
The Dynamic Tube Calibration Wizard provides detailed steps for this process. In short,
there are 7 steps in tube calibration as follows:
Welcome
Simply click Next to begin tube calibration.
Perturbation Selection
Each perturbation loaded into the template that you have selected is displayed. All
perturbations that are used during the experimentation session that follows should be
calibrated; they are not available for use otherwise. The Deep Inflation and SnapShot
perturbations must be calibrated in order to perform the quality control check of your setup and
ventilator circuit as described further. Ensure that each perturbation is preceeded by a
checkmark as in Figure 3-9 and click Next.
47
FIGURE 3-9: SELECTION OF PERTURBATIONS TO BE CALIBRATED
Closed Preparation
Be sure to block the end of the cannula tightly, and then click Next. If the cannula is
not completely blocked, the calibration may need to be repeated.
Closed Calibration
While the cannula is blocked, the flexiVent executes each perturbation that you
selected. Do not unblock the cannula until all perturbations are complete.
Open Preparation
Unblock the cannula and click Next.
Open Calibration
While the cannula is open to atmosphere, the flexiVent executes each perturbation
again. Do not reposition or remove the cannula until all perturbations are complete.
Calibration Results
The tube calibration results contain important information that can help you
validate your setup. Verify that the Rl (leak resistance) value associated with your
Deep Inflation perturbation is within acceptable range using the results as shown
in Figure 3-10 and the calibration guide in Table 3-1. In addition, examine the Rt
(tube resistance) value associated with the SnapShot perturbation. This value
reflects the resistance of your tracheal cannula or intubation tube and as such, it
should be lower than a typical baseline resistance for the species you are studying.
48
FIGURE 3-10: TUBE CALIBRATION RESULTS
TABLE 3-1: TUBE CALIBRATION GUIDE
Deep Inflation Rl value
(cmH2O.s/mL) should exceed
FX1
FX2
MODULE
FX3
2000
1125
600
FX4
FX5
370
260
If your values are not acceptable, confirm that all tubing connections are tight and re-perform
the calibration making sure the cannula is tightly blocked during the closed calibration. If you
continue to get values that are not within acceptable range, please contact SCIREQ Technical
Support.
The importance of proper calibration cannot be overstated. Any errors or inaccuracies
introduced during calibration become part of the data until the next calibration is performed.
15) The software prompts you to start default ventilation. If you plan to connect a subject right
away, select Yes. Please refer to the flexiVent-Specific Features section of the flexiWare
User Manual for information on customizing ventilation patterns.
49
16) When the software prompts you to start recording data, select Yes if you want to record
data streams for all input channels (e.g. cylinder pressure). Select No if you prefer not to
record data streams (i.e. if your primary interest is dataset outcomes).
17) Connect a subject to the Y-tubing with the same cannula that was used during calibration.
18) Execute perturbation(s) to generate datasets, either by double-clicking the perturbation
name in the perturbation docker or by typing the hotkey for the perturbation. Refer to the
flexiVent-Specific Features section of the flexiWare user manual for perturbation settings
and outcomes.
To automate the execution of perturbations, use a script. There may be scripts preloaded into the template you have selected.
19) After you have completed your experiment involving the current subject, stop ventilation ; if
you have been recording data streams, stop recording .
Once ventilation is stopped, understand that your subject is no longer receiving air.
Data from the present experimentation session may be reviewed immediately. To
review all data contained within the study, you must use the Review & Reporting
module of flexiWare software.
20) Remove the subject.
21) Proceed to either adding another subject to the experimentation session or cleaning the
expiratory line, after which you may end the experimentation session.
To add another subject to the experimentation session, click Edit Site Assignment from
the Edit menu. You may need to define another subject to proceed (per Steps 8 through
11), then you will be prompted to repeat steps 13 through 20 as many times as needed
until you are finished with the experimentation session.
To clean the expiratory line and then exit the experimentation session, follow the steps
detailed in section 5.2.2.
To end the experimentation session, go to the File Menu and select Close Session.
50
4 ACCESSORIES
The flexiVent FX system may be customized for a variety of applications through the
incorporation of one or more accessories. To ensure proper function, the experimentation
template you select must correlate to the accessories in your configuration. For details
regarding template selection, naming convention and customization, refer to the flexiWare user
manual.
4.1
UNIT TRANSDUCERS
Per section 1.2.3, a UNIT contains between one and four sensor elements or other input/output
channels, each permitting the measurement or modification of a physical property. In the case
of UNIT transducers, the sensing elements are intended to monitor and record vital signs (e.g.
body temperature) and other parameters (e.g. pressure). UNIT transducer cables plug into the
auxiliary ports at the rear of the flexiVent FX (see section 2.2).
4.1.1
USING UNIT TRANSDUCERS
UNIT transducers are designed for immediate use without the need for calibrations, amplifiers
or additional signal conditioning. Transducer identification and calibrations are stored within
the UNIT to eliminate uncertainty during experimentation or data review. If you prefer to
manually calibrate a UNIT transducer before each experimentation session, contact SCIREQ
Technical Support and request a custom SDM.
51
4.1.2
EKG
The UNIT transducer for monitoring heart rate is comprised of three leads with alligator clips.
To properly use the transducer, first select a lead configuration from the options listed on the
transducer box. Then, wrap a length of copper braid around the appropriate location on the
subject (e.g. left front limb). Attach the lead to the copper braid using the alligator clip. If
necessary, apply conductive gel or saline to the limb and copper braid contact point. However,
ensure that liquid does not reach the alligator clips; the clips are intended for use in dry
conditions only.
The EKG trace is displayed in real-time and may be recorded in the database. If you wish, you
may use the flexiWare heart rate analyzer to calculate a heart rate from the EKG trace.
4.1.3
BODY TEMPERATURE
The UNIT transducer for monitoring body temperature is a rectal thermometer. Temperature
readings are displayed in real-time and may be recorded in the database.
4.1.4
BLOOD PRESSURE
There are two UNIT transducers available to obtain invasive blood pressure measurements.
Both consist of a digital liquid pressure transducer and a chamber for the blood/liquid interface.
Use of the transducers requires arterial access to the subject in which measurements are taken.
It also requires physiological saline and two three-way stopcocks, not supplied by SCIREQ.
Selection of the appropriate transducer is based on the range of pressures to be measured. The
range for each transducer is available in Table 4-1.
52
TABLE 4-1: RANGE FOR BLOOD PRESSURE TRANSDUCERS
PRODUCT CODE
DESCRIPTION
PRESSURE RANGE
UT-DPL-100
Digital blood/liquid pressure transducer
-10 – 10 kPa (-75 – 75 mmHg)
UT-DPL-400
Digital blood/liquid pressure transducer
-40 – 40 kPa (-300 – 300 mmHg)
To confirm whether or not a transducer is appropriate for a certain application, please contact
SCIREQ Technical Support.
To use the transducer, proceed as follows:
1)
Attach a saline-filled syringe to the transducer via the three-way stopcock that is nearest to
the black box of the transducer.
2) Orient the valve of the stopcock to isolate the transducer from the liquid.
3) Fill the transducer chamber with physiological saline from the syringe, and ensure that
there are no air bubbles in the circuit.
4) Orient the valve of the stopcock to isolate the syringe from the circuit.
5) Connect an arterial line to the other end of the circuit via the three-way stopcock furthest
from the black box of the transducer, and then open it to the transducer chamber. Keep the
entire transducer in a horizontal position near the subject while taking measurements.
Pressure measurements are displayed in real-time and may be recorded in the database.
4.1.5
AUXILIARY PRESSURE
SCIREQ offers a wide array of pressure sensors that are optimized to offer the highest
sensitivity for a given pressure range. Each sensor comprises a pressure sensing element that is
further linearized by a microprocessor. Use of auxiliary pressure transducers may be necessary
if you wish to record pressure in a location outside the module. Applications that use auxiliary
pressure transducer include use of negative pressure forced expirations and imaging, though
monitoring auxiliary pressure in the latter case is not required.
53
Selection of the appropriate transducer is based on the range of pressures to be measured. The
range for each transducer is available in Table 4-2.
TABLE 4-2: RANGE FOR DIGITAL PRESSURE TRANSDUCERS
PRODUCT
CODE
DESCRIPTION
PRESSURE RANGE
UT-DPD-02
Digital precision differential pressure
transducer, 0.2 kPa nominal
-0.2 – 0.2 kPa (-2.0 – 2.0 cmH2O)
UT-DPD-05
Digital precision differential pressure
transducer, 0.5 kPa nominal
-0.5 – 0.5 kPa (-5.0 – 5 .0 cmH2O)
UT-DPD-25
Digital precision differential pressure
transducer, 2.5 kPa nominal
-2.5 – 2.5 kPa (-25.5 – 25.5 cmH2O)
UT-DPD-50
Digital precision differential pressure
transducer, 5.0 kPa nominal
-5.0 – 5.0 kPa (-51.0 – 51.0 cmH2O)
UT-DPD-75
Digital precision differential pressure
transducer, 7.5 kPa nominal
-7.5 – 7.5 kPa (-76.5 – 76.5 cmH2O)
UT-DPD-100
Digital precision differential pressure
transducer, 10.0 kPa nominal
-10.0 – 10.0 kPa (-102 – 102 cmH2O)
UT-DPD-300
Digital precision differential pressure
transducer, 30.0 kPa nominal
-30.0 – 30.0 kPa (-305.9 – 305.9
cmH2O)
To confirm whether or not a transducer is appropriate for a certain application, please contact
SCIREQ Technical Support.
54
4.2
NEBULIZER
The nebulizer available for tight integration with flexiVent is the Aeroneb Lab nebulizer, which is
manufactured by Aerogen (Galway, Ireland). The Aeroneb uses ultrasonic vibrating plate
technology to generate consistently-sized droplets without heat, shear force or propelled air,
making it ideal for biological substances. There are two stock models available, see section 4.2.1
for details. Use of the Aeroneb nebulizer requires that you have the nebulizer, its controller
module and a module-appropriate FX adapter with nebulizer mount. To pre-dry the air entering
the flexiVent FX (i.e. in an effort to minimize aerosol rainout), you will also need a drying tube,
plus a quick-connect and length of tubing with which to connect it.
During aerosol production, rain-out will occur and may accumulate in the FX adapter and/or
Y-tubing. The rain-out must be removed frequently to prevent airflow obstruction as
excessive rain-out may compromise mechanical ventilation of the subject and
measurement accuracy.
When using the FX adapter with nebulizer mount, removal of the nebulizer opens the
ventilator circuit and reduces the volume of air delivered to the subject. If possible, avoid
removing the nebulizer from the mount while a subject is attached. If it is necessary to
remove the nebulizer (e.g. for cleaning), do so quickly and immediately replace the nebulizer
or use a cap to close the ventilator circuit.
To use the Aeroneb nebulizer, proceed as follows:
1)
Connect the beige cable from the controller to the nebulizer.
2) Plug the 7-pin connector of the nebulizer controller into the auxiliary port on the back of the
flexiVent.
3) Add a set of Y-tubing to the FX adapter with the nebulizer mount.
4) Insert the FX adapter into the module.
55
5) Once you initiate the software you must select a template that is configured to include the
Aeroneb nebulizer; templates automatically included with the flexiWare software include
‘AN’ in their filename if they are configured for nebulizer use.
Before you proceed through calibration steps, the nebulizer is primed (run
continuously) per the settings in the template to create a seal through which air will not
escape. As such, be sure you have placed liquid in the nebulizer before proceeding
through this step.
6) Place the nebulizer on the mount of the FX adapter and push down until it is flush with the
adapter base to ensure it is securely fastened.
7) Proceed through the next steps of the start-up sequence (e.g. channel calibration).
Templates configured to include the Aeroneb often come pre-loaded with a script for an inhaled
dose response. If you opt to use this script (or a variation thereof), the triggering of the Aeroneb
is automated. You simply need to load it with the substance as needed during the script
execution.
To remove excess fluid from the Aeroneb reservoir, gently wick it up with a tissue. Take care
that no sharp tools come into contact with the nebulizer screen as this may damage the
Aeroneb.
56
4.2.1
AERONEB SPECIFICATIONS
FINE MIST
STANDARD
MIST
ANP-1100
ANP-1000
3.5 μm
5 μm
> 0.1 mL/min
> 0.2 mL/min
Residual Volume
< 0.2 mL
< 0.2 mL
Mass Median Aerodynamic Diameter (MMAD)
~ 1.8 μm
2.5-3.0 μm
2.0 μm
2.0 μm
Particle Size (VMD – Volume Median Diameter)
Nominal Nebulization Output Rate
Geometric Standard Deviation (GSD)
STANDARDIZING MULTIPLE NEBULIZERS
Adjusting the nebulizer settings can allow two or more similar nebulizers to operate at
approximately the same output rate. For example: If nebulizer A has a slightly higher output
than nebulizer B when run at default duty cycle (50%), you can adjust the settings to effectively
obtain similar outputs from both.
To standardize multiple nebulizers, use the following procedure:
1)
Determine the actual aerosol output rate for each nebulizer by characterizing the
nebulizers in flexiWare.
2) Use the following to determine the new nebulization rate (rNeb) at which one nebulizer will be
operated to match the other one:
rNeb A  rNeb B 
rao B
rao A
57
Example: Aeroneb A has an output of 0.25 mL/min and Aeroneb B has an output of 0.20
mL/min when both are run at a 50% duty cycle. The aerosol output of Aeroneb A can be
reduced by modifying its nebulization settings so that both nebulizers produce equal
amounts of aerosol.
rNeb A  0.5 
0.20
0.25
mL
min
mL
 0.4
min
3) Select the Tools menu → Options and then select Aeroneb from the left column.
4) Modify the Nebulization duty cycle to the calculated percentage (e.g. 40% in above
example).
4.3
CALIBRATION ACCESSORIES
The importance of proper calibration cannot be overstated. SCIREQ has a number of
accessories used only during calibration steps that may be integrated into your system.
4.3.1
PRESSURE MANOMETER
The SCIREQ pressure manometer is a J-column pressure manometer with a range of 0-300
mmH2O. The fluid used with the manometer must have specific gravity = 0.87. The ruler on the
front of the manometer may be adjusted by loosening the silver screws on the ruler and moving
the ruler to the appropriate location. The 0 mmH2O mark must correspond with the bottom of
the fluid meniscus when no pressure is applied. Additional fluid may be ordered if needed; refer
to section 1.2.
58
4.3.2
RAINOUT CHARACTERIZATION KIT
The SCIREQ rain-out characterization kit includes a drying tube and connective tubing as well as
an instruction card. The rainout characterization kit is required when characterizing the
nebulizer in the flexiWare software. To ensure accuracy during the nebulizer characterization, it
is important to replace the drying tube once it has turned pink.
4.3.3
TEST LOAD KITS
The SCIREQ Test Load Kit includes two custom-machined test loads to ensure consistency in
outcomes measured by the flexiVent. One test load will produce respiratory system resistance
(Rrs) outcomes similar to a healthy subject while the other test load produces outcomes similar
to a constricted lung.
4.4
HARDWARE EXTENSION FOR NEGATIVE PRESSURE FORCED
EXPIRATIONS (NPFE)
The SCIREQ hardware extension for negative pressure forced expirations consists of a
negative pressure reservoir and valves that integrate into the configuration of the flexiVent FX.
Use of the extension allows you to measure flows (e.g. peak expiratory flow) and volumes (e.g.
forced expired volume at a specific time point, forced vital capacity) generated during a forced
expiration. The computer-controlled NPFE perturbation may be interspersed with other
flexiVent measurements, which allows for the most comprehensive mechanics assessment
available in a single experiment with a single subject.
59
SCIREQ currently offers the negative pressure forced expiration extension for mice and rats
only. Clients should use an FX module 1 with the mouse extension and an FX4 for the rat
extension.
For details on the hardware set up and use of the hardware extension with the flexiVent, consult
the SCIREQ TechNote 036 and other documentation that accompanies the hardware.
60
4.5
MULTI-SUBJECT EXTENSION (MSX)
The SCIREQ multi-subject extension consists of a multi-subject flow controller and four
plethysmograph chambers. Use of the extension allows you to gather mechanics data in up to
four anesthetized subject in parallel, thereby accelerating data collection and allowing you to
expediently carry out time-sensitive studies, for example when respiratory mechanics
measurements must be obtained within a limited time after birth or an intervention.
SCIREQ currently offers the multiple subject extension for mice only. Clients should be utilizing
an FX module 2 with this extension. For details on the hardware set up and use of the hardware
extension with the flexiVent, consult SCIREQ Technical Support and the MSX user manual.
61
5
CLEANING, DECONTAMINATION
AND MAINTENANCE
Routine cleaning and maintenance of the flexiVent FX system are essential to ensure it
continues to operate properly.
5.1
CYLINDER
The precision ground glass cylinder and graphite piston assembly is the most sensitive part of
the flexiVent. Please note the following to ensure a long lifespan of this component.
The cylinder does not require lubrication and should not be lubricated in any way for
any reason.
The cylinder must not be exposed to particulate matter or aerosols, as this may cause
permanent damage.
If particles, aerosol or other substance accidentally enters the cylinder, stop operation
immediately. The module must be thoroughly cleaned by an authorized representative
or the SCIREQ service team before operation resumes. Contact SCIREQ Customer
Support for additional details.
62
5.2
CLEANING FLOW PATHWAYS AND VALVES
5.2.1
INSPIRATORY PATHWAYS & VALVES
Under normal operating conditions, the refill and inspiratory valves of the flexiVent FX contact
gas only. As such, these valves do not require routine cleaning.
5.2.2
EXPIRATORY PATHWAYS AND VALVE
The expiratory valve of the flexiVent FX is subjected to a number of pollutants, including
humidity, mucus and aerosol particles in the expired air. This valve must be cleaned regularly
to ensure proper performance. We recommend that the expiratory pathway (in which the
expiratory valve is housed) is cleaned at the end of every experiment day.
Additional items required for the cleaning procedure include the following:
a module cleaning kit (product code FV-FXQCS or FV-FXQCL);
a syringe (60 mL or larger);
a beaker or similar container (100 mL or larger);
at least 60 mL soapy water or isopropyl alcohol; and
compressed air (optional).
To clean the expiratory line of an FX module, proceed as follows:
1)
Power on the flexiVent.
2) Launch flexiWare and proceed through start-up for a normal experimentation session. You
may cancel out of the Dynamic Tube Calibration dialogue if you only intend to clean the
expiratory line.
Do not start recording data.
Do not start ventilation.
63
3) Remove the FX adapter.
Do not run fluids through the FX adapter when it is attached to the flexiVent.
4) Connect the quick-connects from the cleaning kit to the expiratory port on the front of the
module (A) and the air/gas exhaust port on the back of the module (B) per Figure 5-1.
FIGURE 5-1: QUICK-CONNECT ATTACHMENT FOR CLEANING
5) Add a length of tubing (C) to each quick-connect per Figure 5-2.
64
FIGURE 5-2: TUBING ATTACHMENT FOR CLEANING
6) Place the beaker at the end of the tubing connected to the air/gas exhaust port per
Figure
FIGURE 5-3: FULL CLEANING CONFIGURATION
5-3.
65
7) Fill the syringe with soapy water or isopropyl alcohol and attach it to the tubing per
Figure 5-3. Flush the alcohol through the tubing and expiratory line then collect it in the
beaker.
Larger modules may require a larger volume of soapy water or isopropyl alcohol to
thoroughly clean the line. As such, use a syringe with greater capacity or repeat Step 7
as needed.
If the instrument has been used heavily or aerosol was delivered during recent
experimentation sessions, repeat Step 7 as needed.
8) Flush out any liquid remaining in the lines by blowing compressed air through the tubing
attached to the front of the module (A).
9) Remove both quick-connects.
10) Replace the FX adapter.
11) Attach a test load (e.g. syringe) to the Y-tubing.
12) Start default ventilation and let it run for approximately 15 minutes to ensure that the
expiratory line is completely dry.
Clean the expiratory valve only. Do not attempt to clean the refill or inspiratory valves.
Do not attempt to clean or dry the expiratory line before completing steps 1-3. The software
must be in a particular stage of an experimentation session, otherwise the expiratory valve
is closed. Attempting to clean or dry the expiratory line with the valve close will damage the
module.
Do not use abrasive or corrosive detergents or mechanical cleaning aids.
Do not dismantle the module or base unit to attempt to clean it.
66
5.3
FX ADAPTER AND Y-TUBING
The FX adapter and Y-tubing come into contact with aerosol particles, mucus and humidity.
Routine cleaning of both is necessary to prevent particle accumulation on the interior surfaces.
To clean the FX adapter and Y-tubing, proceed as follows:
Prepare a solution of mild liquid dish soap and water.
Detach the adapter from the module.
Submerge the adapter in the solution and agitate to ensure that the solution enters all
three flow pathways.
Soak if necessary.
Remove the adapter from the soapy solution and rinse thoroughly with clean water.
Use dry compressed air to thoroughly dry the three adapter flow pathways.
Be sure no droplets remain in the small, middle pathway to the module as this can
result in damage to the module when the adapter is subsequently used for an
experimentation session.
The Y-tubing that connects subjects to the FX adapter is a consumable item that must be
replaced periodically. The initial instrument purchase includes appropriate tubing for each
module that is ordered. Additional Y-tubing kits may be ordered when the initial supply is
depleted.
5.4
EXTERNAL SURFACES
To clean the external surfaces of the flexiVent FX, simply run a damp, non-abrasive cloth across
the surface, using a mild solution of water and liquid dish soap if necessary.
When cleaning the clear panel that covers the piston (Lexan material), use only a clean, nonabrasive cloth. Do not use Windex, Pledge, Isopropyl Alcohol or any alcohol-based cleaning
products. Similarly, do not use harsh soaps.
67
Do not use paper or paper towels on the Lexan panel, as these materials are
abrasive and may damage the surface.
5.5
AERONEB NEBULIZER
The domed aperture plate within the nebulizer pump unit is extremely sensitive and should be
treated with extreme care. Do not apply undue pressure to this mesh plate or use a syringe with
a needle to add the liquid.
The instructions that follow are taken directly from Aerogen’s Aeroneb nebulizer information
sheet. Steps 1 through 4 may suffice for everyday use, but continue through step 7 should you
wish to sterilize your nebulizer between subjects, between experiments or following long
periods during which the nebulizer was not used.
5.5.1
1)
NEBULIZER UNIT (INCLUDING FILLER CAP)
Disconnect the control module and cable from the nebulizer unit.
2) Remove the filler cap from the nebulizer unit.
3) Clean parts with warm water and mild liquid detergent. Rinse thoroughly and air dry.
Do not use abrasive or sharp tools to clean the nebulizer unit.
4) Check for cracks or damage, and replace the unit if any defects are visible.
5) Place the disassembled components into appropriate sterilization wrapping.
The Aeroneb can be autoclaved (details provided by manufacturer Aerogen), however
this may shorten its lifespan. Do not reassemble parts prior to autoclaving.
6) To sterilize, autoclave wrapped parts using steam sterilization pre-vacuum cycle, 132 -135°C
(270 - 275°F) for 3 - 4 minutes with drying cycle. Refer to the autoclave manufacturer’s
instructions if necessary. Please contact SCIREQ Technical Support for additional
information.
68
Prior to the next use, check for cracks or damage, and replace the unit if any defects are
visible.
5.5.2
1)
AERONEB CONTROLLER
Wipe clean with a damp cloth.
2) Check for exposed wiring, damaged connectors, or other defects and replace if any are
visible.
Do not autoclave the Aeroneb controller.
Do not immerse it in water.
5.6
ANNUAL CLEANING AND MAINTENANCE
The flexiVent modules have been designed to be field serviceable. With an inspiratory and
expiratory cartridge, the module was designed to minimize the downtime required for annual
cleaning and maintenance by allowing users to simply replace a cartridge in the comfort of their
own lab in a matter of minutes. We recommend that the expiratory cartridge be replaced once a
year or as needed based on usage. Please contact SCIREQ Customer Support to purchase spare
cartridges.
Note that we also offer a service plan that includes pre-paid cartridges along with an extended
warranty and software subscription. Please contact SCIREQ Customer Support for details.
69
5.7
REPACKAGING, TRANSPORT AND STORAGE
If the instrument is not in use for an extended period of time, it is important to protect it from
damage, at a minimum by covering it, but ideally by repacking it in the shipping materials
originally used to ship it to your location. Once packed, the boxes should be stored in a dry,
temperature-controlled location (see Table A-1). Also, if the instrument or its components need
to be moved from one location to another or shipped, it is important to pack it correctly to avoid
damage during transportation.
To pack the instrument, follow the instructions below as appropriate.
5.7.1
PACKING THE BASE UNIT WITH A MODULE ATTACHED
As noted in section 2.2, the base unit is initially shipped with a module attached. This is a
configuration appropriate for storage and transportation. To proceed with packing the base unit
with a module attached, proceed as follows:
1)
Be sure the flexiVent is off (embedded display is dark, piston is not backlit).
If it is on, press the power button to turn it off.
2) Disconnect all transducers, extensions and auxiliary equipment.
3) Disconnect the Ethernet cable.
4) Disconnect the power supply.
5) Open the base unit’s protective cover and ensure that the module securing screw is not
loose.
6) Move the motor arm (to which the piston is connected) to its left-most position.
7) Unscrew the motor lock thumb screw.
8) Rotate the motor lock counter-clockwise.
9) Insert the motor lock thumb screw to engage the motor lock.
When the motor is locked, the motor arm will not move back and forth.
70
10) Close the base unit cover and secure with a piece of tape or a large rubber band.
11) Place the instrument in a vinyl or cloth bag to prevent scratches to the surface.
12) Place the custom-cut foam pieces over the instrument.
13) Gently lower the instrument into its box.
14) Place foam pieces as needed to pad the bottom of the instrument.
15) The power supply, small accessories and cables may be contained within a small box and
packed with the flexiVent as long as there is adequate padding between it and the
flexiVent.
5.7.2
PACKING A MODULE SEPARATELY
If your instrument includes multiple modules, or if a module must be shipped separately (e.g. for
module maintenance service), it may be necessary to pack a module on its own. To pack a
module separately, proceed as follows:
1)
Remove the module from the base unit per the instructions in section 2.6.2.
2) Carefully disconnect the piston/cylinder set from the module. Not all modules attach to the
piston/cylinder the same way. Refer to the module-appropriate instructions below to
complete this step.
71
FX Module 1, 2 and 3:
3) Remove the screws above and below the cylinder while supporting the cylinder and piston
rod per Figure 5-4.
This step requires a 7/64” hex/allen wrench.
4) Gently pull the cylinder back from the module.
5) Locate the O-ring that is seated between the cylinder and module. Place the O-ring and
screws from the assembly into a container (e.g. envelope or small plastic bag).
FIGURE 5-4: MODULE 1, 2 OR 3 DISASSEMBLY
72
6) Wrap bubble wrap or foam around the piston per Figure 5-5.
FIGURE 5-5: PISTON OF MODULE 1, 2 OR 3 WRAPPED
7) Wrap the entire assembly with hard plastic or pliable metal per Figure 5-6.
FIGURE 5-6: CYLINDER ASSEMBLY OF MODULE 1, 2 OR 3 WRAPPED
8) Secure the cylinder and the wrapped piston to the plastic with tape or rubber bands.
The piston should not move within the cylinder if this is done properly.
9) Secure the wrapped assembly to a piece of rigid plastic or cardboard similar to that shown
in Figure 5-7.
FIGURE 5-7: WRAPPED ASSEMBLY SECURED TO RIGID BOARD
10) Tape the container with the O-ring and screws to the back of the rigid board.
73
11) Place the module in an appropriately sized box with 4-6” of padding on all sides.
12) Place the cylinder in the box such that it is padded on all sides and does not move about.
FX Modules 4 and 5:
3) Gently rotate the cylinder counter-clockwise per Figure 5-8.
4) Gently pull the cylinder back from the module.
5) Locate the O-ring that is seated between the cylinder and module. Place the O-ring into a
container (e.g. envelope or small plastic bag).
FIGURE 5-8: MODULE 4 OR 5 DISASSEMBLY
6) Wrap bubble wrap or foam around the piston per Figure 5-4.
7) Wrap entire assembly with hard plastic or pliable metal per Figure 5-6.
74
8) Secure the cylinder and the wrapped piston to the plastic with tape or rubber bands.
The piston should not move within the cylinder if this is done properly.
9) Secure the wrapped assembly to a piece of rigid plastic or cardboard similar to that shown
in Figure 5-7.
10) Tape the container with the O-ring and screws to the back of the rigid board.
11) Place the module in an appropriately sized box with 4-6” of padding on all sides.
12) Place the cylinder in the box such that it is padded on all sides and does not move about.
5.8
REPLACEMENT COMPONENTS
A selection of components used with the flexiVent FX need to be replaced periodically. To order
replacements, contact SCIREQ Customer Relations. The following table includes a listing of
frequently replaced products:
TABLE 5-1: LISTING OF REPLACEMENT PRODUCTS
PRODUCT CODE
DESCRIPTION
ANP-1000
standard particle size Aeroneb nebulizer
ANP-1100
small particle size Aeroneb nebulizer
FV-FEV1-FT
tubing kit for integration of rotameter into NPFE extension
FV-FEV1-YFX
Y-tubing kit for NPFE extension
FV-FXA-SK123
service kit for FX adapter, Modules 1, 2 and 3
FV-FXA-SK123N
service kit for FX adapter with nebulizer mount, Modules 1, 2 and 3
FV-FXA-SK456
service kit for FX adapter, Modules 4 and 5
FV-FXA-SK456N
service kit for FX adapter with nebulizer mount, Modules 4 and 5
FV-FXM[1-5]-I
flexiVent FX Module [1-5] inspiratory cartridge
75
PRODUCT CODE
DESCRIPTION
FV-FXM[1-5]-E
flexiVent FX Module [1-5] expiratory cartridge
FV-FXQCL
cleaning kit for FX Modules 4 and 5
FV-FXQCS
cleaning kit for FX Modules 1, 2 and 3
FV-FXY-1L
Y-tubing kit for FX Module 1, Luer end (qty 6)
FV-FXY-23L
Y-tubing kit for FX Modules 2 and 3, Luer end (qty 6)
FV-FXY-456B
Y-tubing kit for FX Modules 4 and 5, barbed end (qty 3)
FV-FXY-456L
Y-tubing kit for FX Modules 4 and5, Luer end (qty 3)
UT-EKG-SK
EKG transducer service kit
XS-DRY
replacement drying tube
XS-PM-SK
manometer oil
APPENDIX A:TECHNICAL SPECIFICATIONS
TABLE A-1: SPECIFICATIONS FOR THE FLEXIVENT FX
SPECIFICATION
CONDITIONS
MIN
TYP
MAX
UNITS
NOTES
Dimensions
Depth
22 (8 ¾)
cm (in)
Height
14.5 (5 ¾)
cm (in)
FX Base Unit + Module
41 (16 ¼)
cm (in)
FX Base Unit
6.6 (14.5)
kg (lbs)
3
FX Base Unit Power
Supply
1.1 (2.5)
kg (lbs)
3
FX Modules 1 – 3
2.7 (6)
kg (lbs)
3
FX Modules 4 – 5
4.1 (9)
kg (lbs)
3
Resolution
320 x 240
Pixels
Width
Weight
Display
Size
9 (3 ½)
Available volume
Modules FX1 – FX5
1.56
cm (in)
Ventilation
Module Stroke
Volume
51.7
mL
1
SPECIFICATION
CONDITIONS
MIN
TYP
MAX
UNITS
Used volume
Modules FX1 – FX5
1.5
Ventilation
frequency
Based on 1:1 I:E (max
varies with subject
weight)
6
Ventilation tidal
volume
Modules FX1 - FX5
Subject size
Modules FX1 - FX5
Control modes
Software controlled mechanical and pressure controlled
ventilation
Gas supply
Room air or non-flammable mixed gas
51
600
10
8
I:E ratio
1:10
Number of
subjects
measured
simultaneously
1
PEEP
0
Pressure range
mL
3
6
mL/kg
5
g
1
2:1
6
4
2
20
cmH2O
8
-80
80
cmH2O
7
DC
50
Hz
10
mL/kg
1
mL/kg
Respiratory Mechanics
Oscillation
frequency
Oscillation
amplitude
Up to 20% full stroke
3-20Hz
up to 50 Hz
1
br/min
5,100
2:3
NOTES
SPECIFICATION
CONDITIONS
MIN
TYP
MAX
UNITS
NOTES
Environment
Operating
temperature
Operating and
storage humidity
21
non-condensing
Storage and
transport
temperature
40
°C
90%
-20
Altitude
70
2000 (6560)
°C
m (ft)
Electronics
Power supply
output voltage
24
V DC
Power supply
output current
9.2
A
Power supply
input range
110
Power
Power supply
frequency
Sampling
frequency
For 4 channels of data,
which is typical of an
FX flexiVent
240
V AC
221
W
50/60
Hz
256
Hz
9
SPECIFICATION
CONDITIONS
MIN
Inspiratory and
Expiratory ports (FX
adapter/Y-tubing)
1/8
Air/gas intake and
exhaust
1/8
TYP
MAX
UNITS
NOTES
3/8
in dia.
1
3/8
in dia.
1
Connections
Gas port size
Auxiliary ports
(Available UnitWise
transducer power
entry & data I/O)
Power - Voltage
12
V
Power - Current
0.25
mA
Data - Voltage
5
V
Data - Current
0.032
mA
Lemo, DC Power &
I/O, 7-pin snap & lock
connectors,
compatible with UNIT
transducers
4
Channels per Auxiliary
1
port
Sync ports
(Multi-Instrument
synchronization)
Ethernet port
SMA connectors, 3.3
V, 0.024A
10/100 Ethernet
network cable, +/-2.5V
qty
4
1 input
1 output
qty
1
qty
SPECIFICATION
Power supply
(Main flexiVent FX
power entry)
CONDITIONS
MIN
TYP
MAX
UNITS
Kycon, DC Power
connector, 4 pin DIN
R7B female, Snap &
Lock on flexiVent
1
qty
IEC320C14 male on
power supply
1
qty
NOTES
4
Notes:
1
See Table A-2.
2
4 subjects can be studied with the extension for multiple subjects.
3 The FX base unit ships with one module attached; additional modules ship separately.
4 Appropriate AC power cord typically provided only to direct SCIREQ clients in North
America. Clients served by a distribution partner may receive cords through their
distributor. Direct clients outside of North America will need to obtain an appropriate cord.
5
Ventilation tidal volume is configurable in the software. Tidal volume is limited by the
stroke volume of the module in use, so refer to Table A-2 for details.
6 The I:E ratio in the software is expressed as a percentage of the exhalation time that the
inhalation time lasts, e.g. the default 2:3 I:E ratio is expressed as 67%, a 1:1 ratio is expressed
as 100%.
7
Linearity of the transducers is only guaranteed up to ± 76.2 cmH2O
8 The lower limit for computer-controlled PEEP is on the order of 0.25-0.5 cmH2O and may
vary slightly from one configuration to the next. The minimum of 0 cmH2O reflected in the
table is achievable if the option for PEEP control is set to ‘External’ in the ventilation
pattern definition dialogue.
9 Alternate sampling frequencies are available and included in some pre-configured
templates (e.g. those for use with the negative pressure forced expiration hardware). Use
of alternate sampling frequencies may require increased computer RAM and/or
modifications to the real time data view. Please contact Technical Support for more details.
TABLE A-2: MODULE SELECTION TABLE ACCORDING TO STROKE VOLUME AND APPROXIMATE
SUBJECT WEIGHT
MAXIMUM1
MAXIMUM WEIGHT (g) FOR
WEIGHT (g)
VENTILATION
INTAKE AND
1
STROKE MINIMUM FOR LARGE
AND SMALL Y-TUBING EXHAUST
VOLUME WEIGHT AMPLITUDE2
AMPLITUDE3 PORT SIZE PORT SIZE
4
MODULE
(mL)
(g)
MANOEUVRES MANOEUVRES
(in)
(in)
1
FX1
1.53
8
30
150
1/8
1/8 (barbed)
FX2
4.22
20
85
420
3/16
1/8 (barbed)
FX3
12.4
60
240
1,230
3/16
1/8 (barbed)
FX4
28.2
150
560
2,810
3/8
1/4 (barbed)
FX5
51.7
260
1,000
5,100
3/8
1/4 (barbed)
Note:
1
Minimum and maximum weight ranges are subject to change with further development and
are listed for healthy subjects.
2
Estimated using 50 mL/kg ratio.
3 Estimated using 10 mL/kg ratio.
4 Intake and exhaust ports use Colder PMC/PLC connectors. FX Modules are shipped with
mating fittings for easy connection to standard tubing size
Our Customer Service and Technical Support departments are happy to assist you during business
days from 9 am to 5 pm Eastern Standard Time. Most inquiries are addressed within one business
day if not immediately.
TELEPHONE/FAX:
For all inquiries, dial 514.286.1429 and we will direct your call. To contact us by fax, please dial
514.286.1627
EMAIL:
To reach us by email, please use the following addresses:
For Customer Service:
info@scireq.com
For Technical Support:
TechSupport@scireq.com
INTERNET:
A large amount of product and support information is available at any time via our website:
www.scireq.com. Log in to the Downloads section for specific product support information and to
download software updates and other useful files.
SCIREQ Scientific Respiratory Equipment Inc.
6600 rue St-Urbain, Suite 300
Montreal, Quebec, Canada H2S 3G8
T. 514.286.1429
F. 514.286.1627
www.scireq.com
This manual, as all SCIREQ documentation, is believed to be accurate and free of errors at the time of print.
However, all product specifications and other information are subject to change without notice. Some product
features are projected and may not be available immediately. Expected release dates, where provided, are
estimates that are subject to change without notice.
© SCIREQ Scientific Respiratory Equipment Inc. 2014