11-1 The Work of Gregor Mendel 11-1 The Work of Gregor Mendel Slide 1 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Gregor Mendel’s Peas Gregor Mendel’s Peas Genetics is the scientific study of heredity. Gregor Mendel was an Austrian monk. His work was important to the understanding of heredity. Mendel carried out his work with ordinary garden peas. Slide 2 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Gregor Mendel’s Peas Mendel knew that • the male part of each flower produces pollen, (containing sperm). • the female part of the flower produces egg cells. Slide 3 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Gregor Mendel’s Peas During sexual reproduction, sperm and egg cells join in a process called fertilization. Fertilization produces a new cell. Pea flowers are self-pollinating. Slide 4 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Gregor Mendel’s Peas Mendel had true-breeding pea plants that, if allowed to self-pollinate, would produce offspring identical to themselves. Cross-pollination Mendel was able to produce seeds that had two different parents. Slide 5 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Genes and Dominance Genes and Dominance A trait is a specific characteristic that varies from one individual to another. Mendel studied seven pea plant traits, each with two contrasting characters. He crossed plants with each of the seven contrasting characters and studied their offspring. Slide 6 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Genes and Dominance Each original pair of plants is the P (parental) generation. The offspring are called the F1, or “first filial,” generation. The offspring of crosses between parents with different traits are called hybrids. Slide 7 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Genes and Dominance Mendel’s F1 Crosses on Pea Plants Slide 8 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Genes and Dominance Mendel’s Seven F1 Crosses on Pea Plants Mendel’s F1 Crosses on Pea Plants Slide 9 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Genes and Dominance Mendel's first conclusion was that biological inheritance is determined by factors that are passed from one generation to the next. Today, scientists call the factors that determine traits genes. Slide 10 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Genes and Dominance Each of the traits Mendel studied was controlled by one gene that occurred in two contrasting forms that produced different characters for each trait. The different forms of a gene are called alleles. Mendel’s second conclusion is called the principle of dominance. Slide 11 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Genes and Dominance The principle of dominance states that some alleles are dominant and others are recessive. Slide 12 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Genes and Dominance Mendel’s F1 Crosses on Pea Plants Slide 13 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Segregation Segregation Mendel crossed the F1 generation with itself to produce the F2 (second filial) generation. The traits controlled by recessive alleles reappeared in one fourth of the F2 plants. Slide 14 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Segregation Mendel's F2 Generation P Generation Tall Short F2 Generation F1 Generation Tall Tall Tall Tall Tall Short Slide 15 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Segregation The reappearance of the trait controlled by the recessive allele indicated that at some point the allele for shortness had been separated, or segregated, from the allele for tallness. Slide 16 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Segregation Mendel suggested that the alleles for tallness and shortness in the F1 plants segregated from each other during the formation of the sex cells, or gametes. Slide 17 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Segregation Alleles separate during gamete formation. Slide 18 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel 11-2 Probability and Punnett Squares 11-2 Probability and Punnett Squares Slide 19 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Genetics and Probability Genetics and Probability The likelihood that a particular event will occur is called probability. The principles of probability can be used to predict the outcomes of genetic crosses. Slide 20 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Punnett Squares Punnett Squares The gene combinations that might result from a genetic cross can be determined by drawing a diagram known as a Punnett square. Punnett squares can be used to predict and compare the genetic variations that will result from a cross. Slide 21 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Punnett Squares A capital letter represents the dominant allele for tall. A lowercase letter represents the recessive allele for short. In this example, T = tall t = short Slide 22 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Punnett Squares Gametes produced by each F1 parent are shown along the top and left side. Slide 23 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Punnett Squares Organisms that have two identical alleles for a particular trait are said to be homozygous. Organisms that have two different alleles for the same trait are heterozygous. Homozygous organisms are true-breeding for a particular trait. Heterozygous organisms are hybrid for a particular trait. Slide 24 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Punnett Squares All of the tall plants have the same phenotype, or physical characteristics. The tall plants do not have the same genotype, or genetic makeup. One third of the tall plants are TT, while two thirds of the tall plants are Tt. Slide 25 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Punnett Squares The plants have different genotypes (TT and Tt), but they have the same phenotype (tall). TT Homozygous Tt Heterozygous Slide 26 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Probability and Segregation Probability and Segregation One fourth (1/4) of the F2 plants have two alleles for tallness (TT). 2/4 or 1/2 have one allele for tall (T), and one for short (t). One fourth (1/4) of the F2 have two alleles for short (tt). Slide 27 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Probabilities Predict Averages Probabilities Predict Averages Probabilities predict the average outcome of a large number of events. Probability cannot predict the precise outcome of an individual event. In genetics, the larger the number of offspring, the closer the resulting numbers will get to expected values. Slide 28 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel 11-3 Exploring Mendelian Genetics 11–3 Exploring Mendelian Genetics Slide 29 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Independent Assortment Independent Assortment To determine if the segregation of one pair of alleles affects the segregation of another pair of alleles, Mendel performed a two-factor cross. Slide 30 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Independent Assortment The Two-Factor Cross: F1 Mendel crossed true-breeding plants that produced round yellow peas (genotype RRYY) with true-breeding plants that produced wrinkled green peas (genotype rryy). RRYY x rryy All of the F1 offspring produced round yellow peas (RrYy). Slide 31 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Independent Assortment The alleles for round (R) and yellow (Y) are dominant over the alleles for wrinkled (r) and green (y). Slide 32 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Independent Assortment The Two-Factor Cross: F2 Mendel crossed the heterozygous F1 plants (RrYy) with each other to determine if the alleles would segregate from each other in the F2 generation. RrYy × RrYy Slide 33 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Independent Assortment The Punnett square predicts a 9 : 3 : 3 :1 ratio in the F2 generation. Represents: Independent Assortment Slide 34 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Independent Assortment The alleles for seed shape segregated independently of those for seed color. This principle is known as independent assortment. Genes that segregate independently do not influence each other's inheritance. Slide 35 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Independent Assortment The principle of independent assortment states that genes for different traits can segregate independently during the formation of gametes. Independent assortment helps account for the many genetic variations observed in plants, animals, and other organisms. Slide 36 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel A Summary of Mendel's Principles A Summary of Mendel's Principles • Genes are passed from parents to their offspring. • If two or more forms (alleles) of the gene for a single trait exist, some forms of the gene may be dominant and others may be recessive. Slide 37 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel A Summary of Mendel's Principles • In most sexually reproducing organisms, each adult has two copies of each gene. These genes are segregated from each other when gametes are formed. • The alleles for different genes usually segregate independently of one another. Slide 38 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Beyond Dominant and Recessive Alleles Some alleles are neither dominant nor recessive, and many traits are controlled by multiple alleles or multiple genes. Slide 39 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Beyond Dominant and Recessive Alleles Incomplete Dominance When one allele is not completely dominant over another it is called incomplete dominance. In incomplete dominance, the heterozygous phenotype is between the two homozygous phenotypes. Slide 40 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Beyond Dominant and Recessive Alleles RR A cross between red (RR) and white (WW) four o’clock plants produces pinkcolored flowers (RW). WW Slide 41 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Beyond Dominant and Recessive Alleles Codominance In codominance, both alleles contribute to the phenotype. In certain varieties of chicken, the allele for black feathers is codominant with the allele for white feathers. Heterozygous chickens are speckled with both black and white feathers. The black and white colors do not blend to form a new color, but appear separately. Slide 42 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Beyond Dominant and Recessive Alleles Multiple Alleles Genes that are controlled by more than two alleles are said to have multiple alleles. An individual can’t have more than two alleles. However, more than two possible alleles can exist in a population. A rabbit's coat color is determined by a single gene that has at least four different alleles. Slide 43 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Beyond Dominant and Recessive Alleles Different combinations of alleles result in the colors shown here. KEY C= full color; dominant to all other alleles cch = chinchilla; partial defect in pigmentation; dominant to ch and c alleles ch = Himalayan; color in certain parts of the body; dominant to c allele ch h, cor hc chc h, or AIbino: Chinchilla: Himalayan: cc cCC, chcc, , hor cchCc c Full color: Ccch ,cch Cc Copyright Pearson Prentice Hall c = albino; no color; recessive to all other alleles Slide 44 of 32 End Show 11-1 The Work of Gregor Mendel Slide 45 of 32 End Show 11-1 The Work of Gregor Mendel Beyond Dominant and Recessive Alleles Polygenic Traits Traits controlled by two or more genes are said to be polygenic traits. Skin color in humans is a polygenic trait controlled by more than four different genes. Slide 46 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel AaBbCc aabbcc Aabbcc AaBbcc AaBbCc AaBbCc AABbCc AABBCc AABBCC 20/64 15/64 Fraction of progeny LE 14-12 6/64 1/64 Slide 47 of 32 End Show 11-1 The Work of Gregor Mendel Applying Mendel's Principles Applying Mendel's Principles Thomas Hunt Morgan used fruit flies to advance the study of genetics. Morgan and others tested Mendel’s principles and learned that they applied to other organisms as well as plants. Slide 48 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel 11-4 Meiosis 11-4 Meiosis Slide 49 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Each organism must inherit a single copy of every gene from each of its “parents.” Gametes are formed by a process that separates the two sets of genes so that each gamete ends up with just one set. Slide 50 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Chromosome Number Chromosome Number All organisms have different numbers of chromosomes. A body cell in an adult fruit fly has 8 chromosomes: 4 from the fruit fly's male parent, and 4 from its female parent. Slide 51 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Chromosome Number These sets of chromosomes are homologous. Each of the 4 chromosomes that came from the male parent has a corresponding chromosome from the female parent. Slide 52 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Chromosome Number A cell that contains both sets of homologous chromosomes is said to be diploid. The number of chromosomes in a diploid cell is sometimes represented by the symbol 2N. For Drosophila, the diploid number is 8, which can be written as 2N=8. Slide 53 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Chromosome Number The gametes of sexually reproducing organisms contain only a single set of chromosomes, and therefore only a single set of genes. These cells are haploid. Haploid cells are represented by the symbol N. For Drosophila, the haploid number is 4, which can be written as N=4. Slide 54 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Phases of Meiosis Phases of Meiosis Meiosis is a process of reduction division in which the number of chromosomes per cell is cut in half through the separation of homologous chromosomes in a diploid cell. Slide 55 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Phases of Meiosis Meiosis involves two divisions, meiosis I and meiosis II. By the end of meiosis II, the diploid cell that entered meiosis has become 4 haploid cells. Slide 56 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Phases of Meiosis Meiosis I Interphase I Meiosis I Prophase I Metaphase I Anaphase I Telophase I and Cytokinesis Slide 57 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Cells undergo a round of DNA replication, forming duplicate chromosomes. Phases of Meiosis Interphase I Slide 58 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Each chromosome pairs with its corresponding homologous chromosome to form a tetrad. Phases of Meiosis MEIOSIS I Prophase I There are 4 chromatids in a tetrad. Slide 59 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Phases of Meiosis When homologous chromosomes form tetrads in meiosis I, they exchange portions of their chromatids in a process called crossing over. Crossing-over produces new combinations of alleles. Slide 60 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Spindle fibers attach to the chromosomes. Phases of Meiosis MEIOSIS I Metaphase I Slide 61 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel The fibers pull the homologous chromosomes toward opposite ends of the cell. Phases of Meiosis MEIOSIS I Anaphase I Slide 62 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Nuclear membranes form. Phases of Meiosis MEIOSIS I Telophase I and Cytokinesis The cell separates into two cells. The two cells produced by meiosis I have chromosomes and alleles that are different from each other and from the diploid cell that entered meiosis I. Slide 63 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Phases of Meiosis Meiosis II The two cells produced by meiosis I now enter a second meiotic division. Unlike meiosis I, neither cell goes through chromosome replication. Each of the cell’s chromosomes has 2 chromatids. Slide 64 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Phases of Meiosis Meiosis II Telophase I and Cytokinesis I Meiosis II Prophase II Metaphase II Anaphase II Telophase II and Cytokinesis Slide 65 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Meiosis I results in two haploid (N) daughter cells, each with half the number of chromosomes as the original cell. Phases of Meiosis MEIOSIS II Prophase II Slide 66 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel The chromosomes line up in the center of cell. Phases of Meiosis MEIOSIS II Metaphase II Slide 67 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel The sister chromatids separate and move toward opposite ends of the cell. Phases of Meiosis MEIOSIS II Anaphase II Slide 68 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Meiosis II results in four haploid (N) daughter cells. Phases of Meiosis MEIOSIS II Telophase II and Cytokinesis Slide 69 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Gamete Formation Gamete Formation In male animals, meiosis results in four equal-sized gametes called sperm. Slide 70 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Gamete Formation In many female animals, only one egg results from meiosis. The other three cells, called polar bodies, are usually not involved in reproduction. Slide 71 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Comparing Mitosis and Meiosi Comparing Mitosis and Meiosis Mitosis results in the production of two genetically identical diploid cells. Meiosis produces four genetically different haploid cells. Slide 72 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Comparing Mitosis and Meiosi Mitosis • Cells produced by mitosis have the same number of chromosomes and alleles as the original cell. • Mitosis allows an organism to grow and replace cells. • Some organisms reproduce asexually by mitosis. Slide 73 of 32 Copyright Pearson Prentice Hall End Show 11-1 The Work of Gregor Mendel Comparing Mitosis and Meiosi Meiosis • Cells produced by meiosis have half the number of chromosomes as the parent cell. • These cells are genetically different from the diploid cell and from each other. • Meiosis is how sexually-reproducing organisms produce gametes. Slide 74 of 32 Copyright Pearson Prentice Hall End Show