LIFE AND LIVING Do the 2 worksheets on the rules for drawing scientific diagrams. INSTRUCTIONS: • Everything in blue, must be underlined in pencil in your textbook. • Everything in green must be added to your notes in your textbook in pencil or copied into your script in pen. • Brown indicates an activity CHAPTER 1 (p1): Cells as the basic unit of life • Smallest living part of your body = single cell • There are lots of different types • e.g. muscle cells, nerve cells, bone cells • Different types of cells – have different functions • All living things are made of cells Leaf cells of a plant Cheek cells of a human • Humans are multicellular – made up of many cells • Some organisms are made up of a single cell – unicellular • Uni = one • Cells are very small • Can only be seen by using a microscope Do Act 1 on P1 nr 1-4 CELL STRUCTURE (p2) • Cell = basic structural and functional unit of all living organisms • structural – what it is made up of • functional – doing the “job” • Cells can only be seen under a microscope = microscopic • All cells have the same basic structure • See figure 2 on page 2 A typical cell Cell membrane • • • • Outer boundary of the cell Surrounds the contents FUNCTION: Selectively permeable – controls which materials pass into or out of the cell Cytoplasm • Cells are filled with a jelly-like substance – cytoplasm • Mainly water with structures (like organelles) and materials dissolved or suspended in it (hanging in it) • FUNCTION: • Provides a liquid medium for all the chemical reactions inside the cell Nucleus(p3) Draw this sketch of a nucleus (You can leave out the ribosome label) NOT! Draw on board. • DNA is found in the nucleus • Specifically in the chromatin material • Nucleus is surrounded by a double nuclear membrane • Nucleoplasm – jelly part inside nuclear membrane • Nuclear pores – tiny gaps in nuclear membrane which allow particles to move between nucleoplasm and cytoplasm • Nucleolus – round body in nucleoplasm • FUNCTIONS of the nucleus: • Controls all the functions of the cell • Stores the genetic/hereditary info • DNA contains information of organism’s inherited characteristics • E.g. eye colour, hair colour • Each person’s DNA is unique • Children inherit a mixture of their mom and dad’s DNA • Accounts for variation within a single species Do the note on the message in cells Organelles(p3) • Perform different functions for the cell • Found in the cytoplasm of cells Mitochondrion(P3) • Draw this sketch NOT! Off board • Also see the micrograph picture on p3 Micrograph picture = photo taken of something seen under a microscope More mitochondria • Mitochondria are found in both plantand animal cells • FUNCTION: • Cellular respiration – energy is released from glucose • This energy (ATP) is used for all activities of cells • Equation for cellular respiration: • Glucose + Oxygen → ATP + water + Carbon dioxide Vacuoles • Fluid-filled sacs that are surrounded by a membrane • This membrane is called: tonoplast • FUNCTION: • Store water and substances (sugar, salts) – that can be used by the cell • Animal cells – several small vacuoles or none • Plant cells – one or two large vacuoles Chloroplast • Also see the micrograph picture on p6 (Stick in chloroplast sketch) • Chloroplasts are only found in plant cells of green plants • FUNCTION: • Photosynthesis • Equation for photosynthesis: • Radiant energy(from sun) + water + Carbon dioxide → Glucose + Oxygen • Radiant energy is converted into chemical potential energy in glucose More chloroplast sketches: Use these sketches to label your diagram of a chloroplast: • Chloroplasts fall in a group of organelles called plastids • There are 3 types of plastids • Plastids are only found in plant cells Plastids (Copy this table) Colour Where found Function GREEN MAINLY IN LEAVES PHOTOSYNTHESIS CHROMOPLAST RED ORANGE YELLOW RIPE FRUIT ATTRACT ORGANISMS > THEY EAT FRUIT > DISPERSE SEED LEUCOPLAST When exposed to sunlight > change to chloroplasts E.g. potato goes green in light COLOURLESS IN PLANT ORGANS WHICH STORE STARCH E.G. POTATO STORE STARCH (the form in which glucose is stored in a plant) CHLOROPLAST Can become a chromoplast when a green fruit ripens Complete the labels of the 2 sketches on your note on plant- and animal cells Draw your own labelled plant- and animal cells (from overhead) You must start on your Cell model project now (You will get 2 weeks to complete this project) Differences between plant- and animal cells (p5) • Study the 2 sketches on p5 • Plant cells differ from animal cells in the following ways: • Plant cells have a cell wall outside the cell membrane – made of a rigid material called cellulose • FUNCTION: Supports and protects cell. • Animals don’t need a cell wall, because animals have a skeleton for body support • Plant cells have chloroplasts that contain chlorophyll. • Chlorophyll absorbs light energy from the sun – converts it into energy-rich glucose – process of photosynthesis • Glucose is stored as starch in plants • Animals cannot photosynthesise – do not have chloroplasts • Animals get their energy from eating plants or animals that ate the plants • Plant cells have one or two large vacuoles • Animal cells usually do not have vacuoles or if they are present, they are very small • Plant cells have angular shape and animal cells are more rounded • Plant cells have intercellular air spaces between adjacent cell → animal cells don’t Draw and complete the table: Differences between plant- and animal cells CHARACTERISTIC SHAPE CELL WALL VACUOLES INTERCELLULAR AIR SPACE CHLOROPLASTS MITOCHONDRIA PLANT CELL ANIMAL CELL Do Activity 4 on p6 nr 1 and 2 How are cells made? (Stick in note) • Cell uses some food to make new protoplasm • Cell gets bigger • Reaches a certain size – divides into 2 smaller cells • Repeat this process until cells body needs have been reproduced - growth • Process is called Mitosis • Each new cell receives exactly the same genetic information • Body makes more cells at it grows • Fully grown – body goes on making new cells to replace cells worn out and damaged - repair Copy this process of mitosis onto your note: Parent cell is also called a Mother cell Start of mitosis The nucleus now makes a copy of itself – not seen in sketch The mother cell divides into 2 daughter cells The 2 daughter cells are identical to one another as well as the original mother cell More MITOSIS sketches: What is a Microscope?(p7) • An instrument that contains one or more magnifying lenses • Allows us to look at things that are too small to see with the naked eye • Light microscope - uses light to view the specimen • Electron microscope – uses beams of electrons to make an image of the specimen. (Can magnify much more) Parts of the microscope Use the sketch on p7 only as an extra note. Stick in your note on the microscope and study its labels. The light microscope Test your knowledge: Do the worksheet on the Microscope *YOU MUST USE THE LABEL: OCULAR – NOT eyepiece * Carry a Microscope Correctly How to use a light microscope(p8) 1. Place microscope on a level surface. Lowest power objective lens (4x) in place. 2. Turn coarse focussing knob/coarse adjustment screw that objective lens moves away from the stage. 3. Place slide on the stage. Ensure specimen is directly over the hole in the stage. Do not let the slide touch the lens. Continued… 4. Look through the ocular. Turn the coarse focusing knob slowly – stage moves to objective lens. Specimen comes into focus. 5. Use the fine focusing knob to get a clear image of the specimen (final focus) 6. Move the slide to find the best cells to view 7. Carefully use clamps to hold slide in position Continued… 8. Increase the magnification – swing the medium power objective lens (10x) into place by turning the nose piece. 9. Look through ocular and use fine focus knob to fine focus again. 10. Change to high power objective lens (40x) and then repeat step 9. Continued… Determine total magnification: Multiply the magnification of the ocular with the magnification of the objective lens. The discovery of microscopes (p 9) For interest only – NOT TO STUDY What is a wet mount slide?(p10) Wet mount = Specimen is placed in a drop of liquid on a glass slide. The liquid is usually water. A dye may be added to stain the cells or part of the cells to make them more visible when it’s too light in colour. Cover this with a coverslip. How to make a wet mount slide of onion epidermis(p10) • Place a clean glass slide on a flat surface Pull off the epidermis of an onion • Place a small piece of the onion epidermis on the middle of the glass slide. • Place 2 to 3 drops of water or iodine solution (stain) if needed on the onion tissue. • Place the coverslip at an angle on the glass slide. • See sketch on p10 • Coverslip must touch liquid and will flow across the edge of the coverslip. Rest the coverslip on a dissecting needle or pencil at 45º. Lower the coverslip as slowly as possible. Use tissue to dry any excess water. DO NOT WIPE → suck water into tissue In short: What you need to make a slide: • Use the previous info to draw the following sketch: • The parts of a slide • Add these labels: Glass slide, coverslip and specimen. In permanent slides the coverslips are stuck onto the glass slide with special glue and can be used over and over again. You are now ready to do your 3 CELL PRACS The info on p11 is for reading only, NOT TO STUDY Cells in tissues, organs, systems and organism (p12) CELLS • In many different shapes and sizes • Organisms are made up of different numbers of cells CELLS cont… • Microscopic organisms (can only be seen with a microscope): 1. Bacteria - not even a true cell, because it does not have a nucleus CELLS cont… 2. Amoeba – made up of a single cell All the life processes (feeding, respiration, excretion and reproduction are carried out within one cell. Called a unicellular organism. Uni =one CELLS cont… • Macroscopic organisms – humans – made up of thousands of cells = multicellular • In complex organisms groups of cells will specialise(look a certain way) to perform a specific function • They will have the same shape and structure - muscle cells • Able to contract and relax to enable your body to move Muscle cells Tissues, organs, systems and organism (p13) • A group of CELLS that look the same and perform a specific function = TISSUE • A group of tissues working together = ORGAN • Organs working together = SYSTEM • Systems make up = ORGANISM CELLS: Heart cells TISSUE: Heart tissue ORGAN: Heart SYSTEM: Circulatory ORGANISM: Human Do the worksheet on cells, tissues, organs, systems NOT Do Activity 8 on p14 nr 1 (a + c) nr 2 (a + b) Draw 3 of the cells seen in micrograph (a) only Stem cell research (p15) • Stem cells = cells that have not yet developed and can specialise into almost any type of cell in the human body Stem cell cont. • Stem cell research = harvesting stem cells and trying to use it to cure disease • Human stem cells can be harvested in a number of ways: 1. Adult stem cells 2. Umbilical stem cells 3. Embryonic stem cells Stem cell cont. Adult stem cells harvested from the blood, fat tissue and bone marrow: Stem cell cont. Umbilical stem cells are harvested from the blood in the umbilical cord when a baby is born. Embryonic stem cells are harvested from human embryos. Stem cell cont. • Many people are opposed to stem cell research, because the best source is from human embryos • Embryo is destroyed • Considered ethically and morally wrong by many people • They feel a human life is being destroyed when stem cells are harvested from embryos Do Revision activity on p16 (Do everything on p16, except no 3 of the “Test yourself”)