Critically Appraised Topic (CAT) Title: Does post-workout protein intake increase muscular recovery in young men ages 19 yo 28 Name: Cary McKinney Rationale for Topic Selection: Since I was young I've always wanted to be big, strong, and muscular. I used to spend hours looking at bodybuilding magazines, documentaries, and movies. If it had to do with being big, strong, and muscular, I had seen it. When I first started training I had success using what I had learned. Until my stupidity got the best of me and I began to rack up more injuries in the gym than weights. As of now, I am exercise again with modifications, recovering, and getting stronger every day. Even so, I need to be able to get the most efficiency out of both my training and nutrition. I found myself looking back into the old idea of “you should always drink a protein shake post-workout”. I decided with my newfound knowledge of scientific research methods, to learn if this claim was true. Research Question: Question: Does post-workout protein intake increase muscular recovery in young men ages 19 yo 28? P: Young men ages 19 yo 28 I: Post-workout protein C: No post-workout protein O: increased muscular recovery Search History: Thankfully for the topic I have chosen, I was able to find a plethora of studies to match my needs, and as such the searching process was relatively easy for me. First using the CGCC library database input that I only wanted to see peerreviewed scholarly articles that linked to the full text. In addition, I was able to limit the age group of the study results to the ages of eighteen to forty-four years of age. After setting my search parameters I simply used my search terms found below in different combinations to find the necessary studies to complete my research. Search Terms Databases · · · · · · Protein Recovery Post-exercise Post-workout Ages-19-44 Gender-Male · EBSCOhost Citations: Using APA 6th Ed Guidelines, provide the full-text citation of each peer-reviewed journal article reviewed Starkoff, B. E., Lenz, E. K., Mattern, C. O., Too, D., & Byrne, H. K. (2019). “The effect of protein supplementation on recovery from exercise-induced muscle damage.” Medicine & Science in Sports & Exercise, 51(Supplement), 546. https://www.asep.org/asep/asep/JEPonlineFEBRUAR Y2020_Starkoff.pdf Roberts, J., Zinchenko, A., Suckling, C., Smith, L., Johnstone, J., & Henselmans, M. (2017). “The short-term effect of high versus moderate protein intake on recovery after strength training in resistance-trained individuals.” Journal of the International Society of Sports Nutrition, 14(1). https://jissn.biomedcentral.com/articles/10.1186/s1297 0-017-0201-z Gee, T. I., Woolrich, T. J., & Smith, M. F. (2019). “Effectiveness of whey protein hydrolysate and milkbased formulated drinks on recovery of strength and power following Acute resistance exercise.” Journal of Human Kinetics, 68(1), 193-202. doi:https://content.sciendo.com/view/journals/hukin/68/ 1/article-p193.xml?product=sciendo Summary of Studies: (Starkoff, Lenz, Mattern, Too, Byrne, 2020) Study Design: Randomized controlled trial Setting: Unknown, possibly Valparaiso University, Valparaiso, IN OR The College at Brockport – State University of New York, Brockport, NY Participants: A total of 20 healthy, active males, age 22.1 ± 3.9 Intervention: There were two separate groups during the 10day, double-blinded, randomized, isocaloric design. These were the CHO (carbohydrate only) or CHO-PRO (carbohydrate and protein) group. Subjects were based on their estimated basal metabolic rate (BMR) using the MifflinSt. Jeor equation. On study day two, an eight-day supply of food was provided to each subject to begin consuming on day three through the completion of the study. CHO = The subjects were given supplemental beverages containing either 60 gm of CHO (240 kcals) (CHO condition) CHO-PRO = The subjects were given or an isocaloric beverage with CHO and PRO in a 2:1 ratio containing 40 gm of CHO and 20 gm of PRO (240 kcals) (CHO-PRO condition). Outcome Measures: Following a two hour fast, the subjects were asked to consume 120 kcals of their supplement one hr before the baseline testing / EIMD (exercise-induced muscle damage) exercise session. Upon arrival to the laboratory, a blood sample was acquired, and lower body muscle performance was assessed and a baseline level of muscle soreness was determined. Finally, at the end of the visit, the subjects completed the EIMD protocol. The EIMD exercise session consisted of 100 drop jumps from a 0.6-m box. Participants jumped from the box to the ground, performing 90° of knee flexion upon landing, and then immediately jumped up as high as they could. There was a 10 sec of rest between each jump and 2 min of rest between each set of 20 jumps. Serum CK concentration and Serum myoglobin concentration were measured via a blood sample. Main Findings: Findings indicate that CHO or CHO-PRO supplements are similar in terms of recovery from EIMD. However, based on current literature post-EIMD supplementation may not be necessary if the diet is of adequate quality. (Roberts, Zinchenko, Suckling, Smith, Johnstone & Henselmans, 2017) Study Design: Randomized controlled trial Setting: Anglia Ruskin University, in Cambridge, England Participants: Sixteen individuals (9 men, 7 women) (two participants were excluded from the final analysis due to dietary non-compliance) ages 31 ± 6 Intervention: Participants were randomly assigned to a tenday equal calorie period of either moderate (1.8 g./kg) or high (2.9 g./kg) total protein intake (PROMOD and PROHIGH respectively). At the end of the first dietary period, participants returned to habitual intake patterns for 24 h before beginning the opposing dietary condition. PROMOD = Participants followed an individual maintenance calorie intake based on the formula of Katch-McArdle, macronutrient amounts were 40% carbohydrate, 25 or 35% protein, and 25 to 35% fat, with the moderate protein group consuming 1.8g per Kg of bodyweight. PROHIGH = Participants followed an individual maintenance calorie intake based on the formula of Katch-McArdle, macronutrient amounts were 40% carbohydrate, 25 or 35% protein, and 25 to 35% fat, with the high protein group consuming 2.9g per Kg of bodyweight. Outcome Measures: Body density including body fat, fat-free mass and fat mass values. The following exercises were used for baseline measurement at one rep max (five attempts were allowed to reach one rep max), squat, bench press, and bent over row. TNF-α was also assessed by a multiplexed electrochemical luminescence immunoassay. Main Findings: A high protein diet did not improve markers of muscle damage or soreness following repeated days of intensive training when daily calorie and peri-exercise protein intake was controlled. This study indicates that moderate protein intakes may be sufficient for resistance-trained individuals during acute periods of intensive exercise. (Gee, Woolrich and Smith, 2019) Study Design: Randomized controlled trial Setting: University of Lincoln in Lincoln, England Participants: thirty resistance-trained males from a university population age: 25.2 ± 5.5 years old Intervention: Participants were evenly and randomly assigned to one of three groups: the whey hydrolysate-based drink group (WH), the milk-based drink group (MB), or the flavored dextrose/carbohydrate group (CHO). WH = All drink supplements were administered 10 min postexercise, participants consumed their drink within a 10 min period. The WH contained strawberry flavored whey hydrolysate (39 g of Strawberry flavor Hydrolysed Whey Protein Isolate with 97 g dextrose powder as a source of carbohydrates. MB = All drink supplements were administered 10 min postexercise, participants consumed their drink within a 10 min period. The MB condition was two pre-mixed bottles of a commercially available low-fat berry-flavored milkshake. CHO = All drink supplements were administered 10 min postexercise, participants consumed their drink within a 10 min period. For the CHO condition, 133 g dextrose powder was provided with a strawberry-flavored non-calorie sweetener. Outcome Measures: Body mass and height were measured. Participants then completed a total of five bodyweight squats and subsequently used the visual analog scale to transcribe their perceived levels of muscular soreness whilst executing the squats. Each participant then completed a five minute warm-up on a cycle ergometer at 60 W (60 r·min-1), followed by three individual countermovement jumps and seated medicine-ball throws. Following this, participants carried out another five minute warm-up on the cycle ergometer prior to isokinetic assessment. The isokinetic assessment included completing five maximal-effort knee flexion and extension actions at 60°/s using the dominant-leg after two warm-up sets, which completed the protocol of assessments. Main Findings: The study Effectiveness of Whey Protein Hydrolysate and Milk-Based Formulated Drinks on Recovery of Strength and Power Following Acute Resistance Exercise found that there was no apparent difference in recovery response between WH, MB and CHO condition groups following intense resistance exercise. Summary of Evidence: I previously read in the study The Effect of Protein Supplementation on Recovery From Exercise-Induced Muscle Damage that the literature surrounding whether or not post-exercise protein intake was beneficial for recovering from exercise-induced damage was still inconclusive. However, based on the literature I have reviewed it seems that it is in fact decisively not beneficial to aid muscular recovery. Two different studies compared the effects of carbohydrate only post-workout beverage and the effects of a protein and carbohydrate combined beverage and both studies found that there was no significant difference in the recovery of participants from post-exercise muscular damage (Starkoff, Lenz, Mattern, Too, Byrne, 2020) (Gee, Woolrich and Smith, 2019). In both of these studies, the caloric and macronutrient consumption of participants was controlled by the researchers. In the case of the study The Effect of Protein Supplementation on Recovery From Exercise-Induced Muscle Damage, researchers provided food to the subjects on the second day of the study and asked them to consume only said food (or additional vegetables under the supervision of researchers if need be) for the remind eight-day duration of the study (Starkoff, Lenz, Mattern, Too, Byrne, 2020), whereas in the study The short-term effect of high versus moderate protein intake on recovery after strength training in resistance-trained individuals, researchers asked the participants to meet a certain macronutrient goal and had them track their intake on the app “My Fitness Pal” (Roberts, Zinchenko, Suckling, Smith, Johnstone & Henselmans, 2017). In addition, I reviewed a study on the use of high or moderate protein diets in recovery from exercise-induced muscular damage to see if a link could be found between the amount of protein taken in and recovery. Again no conclusive evidence was found that the high vs moderate protein group had better muscular recovery post-exercise-induced damage (Roberts, Zinchenko, Suckling, Smith, Johnstone & Henselmans, 2017). All studies point to the conclusion that in the case of an adequate quality diet, post-exercise consumption of a protein beverage does not contribute to muscular recovery from said exercise-induced damage. Clinical Recommendation: Additional References: Young men ages nineteen to twenty-eight would most likely not benefit from the use of a post-workout protein drink supplement for recovery. However, there is strong evidence that an increased amount of protein in the diet will support muscle growth. As such it is important to note that although post-workout protein drink supplementation did not support muscular recovery from exercised induced damage, these studies did not look at any other effects of post-workout protein supplementation such as increased muscle growth. N/A