1
outline
Generation
Generation of
of the
the CRISPR/Cas9-mediated
CRISPR/Cas9-mediated knock-in
knock-in system
system targeting
targeting
the
the promoter
promoter of
of Oct4
Oct4
Transcriptional regulation of Oct4 gene expression via HDR-mediated
gene correction
Combinatorial alteration of the CpG sites in CR1 and the upper
promoter region
Effects of targeted demethylation using dCas9-Tet1 modules on Oct4
gene activation
Synergetic effects of altered histone modifications and DNA methylation
on Oct4 expression
2
Generation of the CRISPR/Cas9-mediated knock-in system targeting the promoter
of Oct4
Research object：Oct4 promoter in NIH3T3 cells
Transcriptional regulation of Oct4 gene expression via HDR-mediated gene
correction
Combinatorial alteration of the CpG sites in CR1 and the upper promoter region
Effects of targeted demethylation using dCas9-Tet1 modules on Oct4 gene
activation
Significant increase of Oct4 mRNA was not also observed in a
cell clone with a stable overexpression of sgRNA1/dCas9Tet1
Synergetic effects of altered histone modifications and DNA methylation on Oct4
expression
DNMT: DNA
甲基转移酶
Apicidin:组蛋白脱乙酰基
酶抑制剂
UNC0638:组蛋白甲基转
移酶抑制剂
The dCas9-Tet1 system can effectively activate a
transcriptionally quiescent gene via targeted DNA
demethylation, when supplemented by both
histone H3 acetylating- and Histone H3K9
demethylating-agents.