301 892 Seminar in Materials Science and Nanotechnology November 4, 2016 (13.00-13.30) The asymmetric gold nanoparticle aggregation for colorimetric sensors with high detection. Piyaporn Matulakun Materials Science and Nanotechnology, Faculty of Science, Khon Kean University, Khon Kean, THAILAND Email : piyapornmatulakun@gmail.com Abstract Gold nanoparticles are non reactive, electrical conductivity, resistance to corrosion or oxidation and stable thiolate bonds to molecules with thiol [1]. Thus, the gold nanomaterials were chosen for sensing material. The colorimetric sensors can provided monitoring of the interaction biomolecules based on aggregation of gold nanoparticles. However, the current colorimetric sensors are low sensitivity and its have limitation in real sample analysis [2]. This article demonstrates a new strategy of colorimetric sensor for DNA detection. The asymmetric gold nanoparticles were utilized as probes which are selective form to dimers without forming large aggregates upon target binding. This strategy exhibited a long-term stability and a more than 2 orders of magnitude wider dynamic range of detection than that of the conventional colorimetric sensors. Because the gab between two neighboring gold nanoparticles was decreased due to the formation of Y shape DNA duplex. In addition, a novel proposed sensor showed to enhance the limlt of detection 10000 times and can be applied for genomic DNA in foodborne pathogens detection [3]. References [1] Dreaden, E.C., et al. (2012) “The golden age: gold nanoparticles for biomedicine”, Chemical Society Reviews, 41(7), pp. 2740-2779. [2] Xia, F., et al. (2010) “Colorimetric detection of DNA, small molecules,proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes”,Proceedings of the National Academy of Sciences, 107(24), pp. 10837–10841. [3] Guo, L., et al. (2013) “Oriented gold nanoparticle aggregation for colorimetric sensors with surprisingly high analytical figures of merit”, Journal of the American Chemical Society, 135, pp. 12338-12345.
