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World Applied Sciences Journal 15 (7): 1039-1045, 2011
ISSN 1818-4952
© IDOSI Publications, 2011
Effect of Kinds of Salt and its Different Levels
on Seed Germination and Growth of Basil Plant
1
Seyed Morteza Zahedi, 2Majid Nabipour, 1Mehrdad Azizi,
1
Hadi Gheisary, 1Mansour Jalali and 3Zohreh Amini
1
Horticulture in Ahvaz Shahid Chamran University, Iran
2
Ahvaz Shahid Chamran University, Iran
3
Biotechnology in Ahvaz Shahid Chamran University, Iran
Abstract: To investigation on effect of salt and it's different levels on germination and growth stage on
basil (Cultivar native mass) two separate experiment were carried out (2010) at lab physiology and
greenhouse of agriculture faculty of Ahvaz shahid chamran university. In first experiment
seven salt (1-KCl, 2-K2SO4, 3-NaHCO3, 4-Na2SO4, 5-CaCl2, 6-NaCl, 7-Na2CO3) with five salinity levels
(EC= 2,4,6,8 and 10 dS/m) and distilled water (control) under germinator condition were used.
This investigation was performed as factorial experiment under Complete Randomized Design with three
replications. It was concluded that effect of treatment on germination percentage, germination speed,
seedling normal and abnormal percentage, vigor1 and vigor2 index had significant different (P=0.05).
In all measurements the highest mean was related to control. Na2CO3 and NaHCO3 had the highest effect to
reduction of above characters. In second experiment all of salts which used in the first experiment with three
levels (EC=2, 6, 10 dS/m) and distilled water (control) were applied. In this experiment plumule and radicle fresh
weight, leaf area, plumule and radicle dry weight, leaf number, radicle and plumule length were measured at the
end of vegetative stage. The result show that vegetative growth reduced with increasing salinity level and the
most negative effect related to Na2SO4 and CaCl2.
Key words: Germination % Salinity % Basil % Vegetative growth % Vigor
INTRODUCTION
Basil (Ocimum basilicum L.) belong to the Lamiaceae
family, Nepetoideae subfamily and the genus sensu lato,
comprises 65 species [1]. Basil is one of the species that
used for the commercial seasoning [2]. Basil is an annual
herb with 50-60 cm plant height and pink and white
flowers. The useful parts of basil plant are leaves and
seeds [3]. Sweet basil is a popular herb and there is grown
for its dry or green aromatic leaves which are used as a
spice or as a flavoring. The spicy herb, a member of the
mint family, is a native of Central Asia and North West
India. It is adapted to growth in warm conditions and
although it is a perennial, plant but used as annual. It is
commonly used in tomato dishes and as a flavoring in
soups, salads, pesto sauce, minced beef and sausages.
This plant is produced commercially in Egypt, France,
Hungary, Mexico, Indonesia and Hawaii. It is grown in
subtropical zone from sea level to altitude of 1800 m.
sweet basil is one of the most widespread spices in the
world. Sweet basil is an important medicinal plant
also used as culinary herb [4]. It is a source of aroma
compounds and essential oils with antifungal [5],
antibacterial, insect repellent and nematocidal activities
[6-9]. Basil has been used as a folk remedy for an
enormous number of ailments, including boredom, cancer,
convulsion, deafness, diarrhea, epilepsy, gout, hiccup,
impotency, insanity, nausea, sore throat, toothaches and
whooping cough 40.
Salinity is defined as the presence of high levels of
minerals (cations K, Mg, Ca, Na and anions NO3, HCO3,
SO4, Cl) in water and soil [10]. High salinity in the root
zone affects the growth of many plant species. A soil is
said to be saline when it has enough of soluble salt to
affect plant growth. Saline soils, (salty soils) can reduce
the water uptake by plants, restrict root growth, cause
marginal burning of the foliage, inhibit flowering, limit
seed germination and reduce fruit and vegetable yields.
Like the most of plants, growth and yield of basil can be
affected by environmental constraintssuch as soil and
Corresponding Author: Seyed Morteza Zahedi, Horticulture in Ahvaz Shahid Chamran University, Iran.
1039
World Appl. Sci. J., 15 (7): 1039-1045, 2011
water salinity [11]. Salinity is one of the most important
limiting factors in production of horticultural crops.
Naturally occurring salt stress is generally due to NaCl
[12]. Sodium chloride is composed of two elements,
sodium and chlorine [13]. Salinity is a major environmental
constraint to crop productivity throughout the arid and
semi-arid regions of the world [14]. Salinity has reached a
level of 19.5% of all irrigated-land (230 million ha of
irrigated land, 45 million ha are salt-affected soils) and
2.1% of dry-land (1500 million ha of dry land agriculture,
32 million are salt-affected soils) agriculture worldwide [1].
Seed germination is a major limiting factor the
establishment of plants under saline conditions. Salinity
may cause significant reductions in the rate and
percentage of germination, which in turn may lead to
uneven stand establishment and reduced crop yields
[14]. Salinity can damage the plant through osmotic effect,
specific toxic effects of ions and disturbance of essential
nutrient uptake [15, 16]. Plant adaptations to salinity
mainly concern tolerance to salt-induced osmotic stress,
tissue tolerance to accumulated Na+ or Cl- ions and
exclusion of these ions at both cell and whole plant level
[17]. In general, enzymic and metabolic activities in plants
are highly influenced by both amount and type of salts
[18]. Photosynthesis depressed has been suggested to
be responsible of growth reduction of salt-stressed plants
[19, 20]. Stomata closure, arising from the osmotic
component of salinity, has been reported to be primarily
responsible for photosynthesis inhibition in some
studies [21, 22]. Generally, salinity problems increase with
increasing salt concentration in irrigation water [23].
More than half of the world ground water supplies are
saline [1]. In order to use of saline water for irrigation,
need to understand how salinity affects a plant
monitor salinity levels constantly to ensure they stay
within the acceptable range and be prepared to accept
lower-than-average yields [24]. The adverse effects of
high concentration of salts for plants are due to the
osmotic retention of water and to specific ionic effects on
the protoplasm.
Since the salinity is one of the most important
problems of tropical and subtropical regions (for example
Khuzestan region) and also according to the soil and
irrigation water test, there is showed that the region has
a variety of salt including sodium chloride, sodium
sulphate, sodium carbonate, chloride calcium, etc.
Therefore, this study was conducted to examine the
influence of salt and its different levels on seed
germination and growth of basil (Ocimum basilicum L.)
plant.
MATERIALS AND METHODS
Laboratory Experiments: In order to determine the effect
of salt and its different levels on germination in basil seed
s, an experiment was conducted in 2010 under a complete
randomize design (CRD) with three replications.
Treatments included seven types of salt and different
levels of salinity, including five levels (2, 4, 6, 8, 10 dS/m)
and distilled water was used as control treatment. In order
to prepare Salt solutions were prepared by sodium
chloride (NaCl), calcium chloride (CaCl2), sodium
carbonate (NaCo3), sodium hydrogen carbonate
(NaHCo3), potassium sulphate (K2So4), sodium sulphate
(Na2So4) and potassium chloride (Kcl).
Preparation of Salts Solution: Salinity levels were
prepared with the following equation [16]:
EC=TDS/640.
EC=electrical conductivity, TDS= concentration of soluble
salts in milligrams per liter.
Bioassays: Dishes were sterilized with distilled water.
Also seeds of Basil were mixed with 2.5% sodium
hypochlorite for 10 minutes then washed seven times with
sterile distilled waterand placed on evenly on filter paper
in sterilized 9cm Petri dishes. Five milliliters from each
solutions was added to Petri dishes and to make control
samples used from distilled water. Seeds allowed to
germinate at 25±1°C for 7days.
Germination percentage was recorded after 7thday.
Germination percentage and Germination rate was
calculated with the following equation:
Germination percentage (%) = (Number of germinated
seeds / Number of total seeds) × 100
Germination rate= N1/D1+N2/D2+...+Ni/Di
That S is the number of germinated seeds, T is the
total number of seeds and Ni is the number of germinated
seeds, per day (Di).
Also, seedling normal and abnormal percentage
and Seedling vigor index were calculated by the
following formula:
Vigor index = Standard of germination percentage×
Average of Seedling length (cm)
Vigor index =Standard of germinationpercentage ×
Average of Seedling weight (g)
1040
World Appl. Sci. J., 15 (7): 1039-1045, 2011
Greenhouse Experiments: In second study on
experiment was conducted in greenhouse like field
condition in Shahid Chamran University of Ahvaz
(Latitude 31°19' 45" N and longitude 48°41' 28" E).
Basil seeds were plented in pots with 1/2soil and 1/2
sand. Greenhouse studies were carried out during the
spring and summer of 2010. Average day/night
temperature, day length and photosynthetically active
radiation were 25/19, 16hr. and 1950 µmol.mG2.secG1,
respectively. In second experiment (growth vegetative
stage test) last seven salts were chosen with three
salinity levels (EC= 2, 6, 10 dS/m) and distilled water as
control (regard laboratory studies: 2 dS/m= lower than
stability of plant; 6 dS/m= middle stability of plant;
10 dS/m= higher thanstability of plant). In a complete
randomized design (CRD) with three replications. Salinity
treatment was applied when plant had four leaves. In this
experiment plumule and radicle fresh weight, leaf area,
plumule and radicle dry weight, leaf number, plumule and
radicle length were measured at the end of vegetative
stage.
Statistical Analysis: The data collected was analyzed
using the SAS and Excel technique under completely
randomized block design (CDR) and the treatment means
were compared by Duncan’s test.
RESULTS AND DISCUSSION
Laboratory Experiment: Growth indices of basil
seeds was significantly affected by salinity (P=0.05)
(Table 1 and 2). Results showed that maximum
germination percentage of basil seeds (95%) obtained in
distilled water but by increasing salts solution prevented
seeds germination decreased. The greatest reduction in
germination observed at NaHCo3. Salinity stress effects
on seed germination via limitation of water absorption by
seeds [25]. The rate of germination was the highest
at the control (distilled water) compared to other salts
(Table 1 and 2). The tendency reduction in germination
rate due to increasing salinity was reported by others
[26-27]. The reduction rate of germination at high salt
levels might be mainly due to osmotic stress [28]. Results
showed that the effect of salts on the percentage of
seedling normal and abnormal was significantly (P=0.05).
Also, the highest seedling vigour ² and ²² were created in
control (distilled water). Ramin (2006) observed that seed
germination and seedling development were similar to
controls at low salinity levels, but abnormal seedling
development was increased and germination reduced at
salinity levels of # 12 dS/m for both, (green and purple)
type of sweet basil [13]. Jamil and Rha (2007) observed
that shoot length, root lengths and dry weight were
decreased with increasing salt stress [29]. It was
concluded that germination and early seedling growth of
seeds of Cowpea were inhibited by increasing salt
concentration. Also in this experiment indicated that
effect of treatment on germination percentage,
germination rate, seedling normal and abnormal
percentage, vigor ² and vigor ²² index had significant
different (P=0.05). The highest mean was related to
control (distilled water). Na2CO3 and NaHCO3 had the
highest effect to reduction of above characters. Results
showed that the destructive effects of NaHCo3 index were
more than other salts. It is assumed that germination rate
and the final seed germination decreased with the
reduction of water movement into the seeds during
imbibitions [30]. Salinity stress can affect to seed
germination through osmotic effects [31]. Salt induced
inhibition of seed germination and could be attributed
to osmotic stress or specific ion toxicity [32]. Germination
percentage decreased significantly by increasing
salinity level. These results are similar in line with
Jeannette et al. [33].
Table 1: Influence of concentration of solution salts on germination and growth parameters in Basil (Laboratory experiments)
Salts
Germination percentage
Germination rate
Seedling abnormal percentage
Seedling normal percentage Vigor1
Vigor2
Kcl
b 33/66
d 57/8
c 4/15
b 6/78
b 53/2
bc 000552/0
K2SO4
b 67
cd 04/9
cd 67/12
b 33/81
d 02/2
b 000668/0
Na2HCo3
c 67/35
f 23/2
b 4/23
c 6/16
g 25/0
d 000118/0
Na2SO4
b 33/66
d 75/8
c 867/13
b 13/86
e 72/1
c 000498/0
CaCl2
b 72
b 619/10
c 93/16
b 067/83
cd 25/2
bc 000608/0
NaCl
b 71
bc 22/10
d 86/13
ab 13/91
bc 35/2
bc 000624/0
Na2CO3
d 33/18
e4
e0
c 8/19
f 59/0
d 000184/0
Control
a 95
a 28/16
a 28/16
a 100
a 43/4
a 00095/0
Different letters refer to significant differences between treatments
1041
World Appl. Sci. J., 15 (7): 1039-1045, 2011
Table 2: Comparison of salts solution averages on germination and growth parameters in Basil (Laboratory experiments)
Salt
Treatment
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Concentration
Germination
Germination
Seedling
Seedling
(EC)
percentage
rate
normal percentage
abnormal percentage
Vigor1
Vigor2
Kcl
2EC=
4EC=
6EC=
8EC=
10EC=
abcdef 33/78
abcdef 33/73
defgh 65
hi 50
hi 50
cd 11/11
cdef 223/10
efghijk 11/8
hijk 11/7
jk 33/6
a 100
a 100
abc 89
bc 33/68
c 67/65
g0
g0
defg 11
bcd 67/31
b 33/34
ab 029/4
cd 149/3
ef 58/2
hij 74/1
lmno 18/1
abc00085/0
bcdefgh00065/0
bcdefgh00063/0
ghi00044/0
j00017/0
K2SO4
2EC=
4 EC=
6 EC=
8 EC=
10 EC=
abcde 80
defgh 65
bcdefg 33/73
fgh 60
gh 33/58
bc 5/12
defghi 9
defghi 22/9
fghijk 833/7
ijk 667/6
a 100
abc 92
abc 88
abc 77
abc 67/79
g0
efg 8
defg 12
bcdef23
bcdefg33/20
bc 60/3
fgh 24/2
hij 96/1
klmn 36/1
nop 92/0
ab00086/0
bcdefgh00065/0
abcdef00073/0
bcdefgh00060/0
efgh00049/0
NaHCO3
2 EC=
4 EC=
6 EC=
8 EC=
10 EC=
bcdefg 70
j 33/18
k0
k0
k0
defgh 33/9
mn 833/1
n0
n0
n0
abc 83
d0
d0
d0
d0
bcdefg 17
a 100
g0
g0
g0
klmno 29/1
p0
p0
p0
p0
bcdefg000594/0
J0
j0
j0
j0
Na2SO4
2 EC=
bcdefgh 33/68
cdef 387/10
abc 33/90
efg 67/9
de 046/3
bcdefgh00062/0
4 EC=
6 EC=
8 EC=
10 EC=
bcdefg 70
abcde 80
gh 667/56
gh 667/56
defgh 667/9
cde 72/10
ghijk 5/7
kl 5/5
ab 33/92
abc 33/87
abc 67/84
abc 76
fg 67/7
cdefg 67/12
bcdefg 33/15
bdef24
ghij 006/2
jkl 69/1
mnop 06/1
op 80/0
defgh00057/0
bcdefgh00062/0
efgh000484/0
j00018/0
CaCL2
2 EC=
4 EC=
6 EC=
8 EC=
10 EC=
ab 667/86
bcdefg 667/71
bcdefg 667/71
efgh 33/63
cdefgh 667/66
ab 77/14
cde 61/10
cdef 21/10
defghij 833/8
defghij 667/8
ab 33/92
abc 33/83
abc 88
abc 81
bc 67/70
fg 67/7
bcdefg 67/16
defg 12
bcdefg 19
bcde 33/29
bcd 56/3
efg 53/2
fgh 28/2
hij 75/1
lmnop 15/1
abcd000817/0
cdefgh00059/0
bcdefgh00060/0
efgh0005/0
efgh00053/0
NaCL
2 EC=
4 EC=
6 EC=
8 EC=
10 EC=
abc 85
bcdefg 70
abcd 33/83
gh 33/58
gh 33/58
ab 72/14
cd 11/11
cdef 387/10
hijk 11/7
fghijk 77/7
abc 33/91
abc 92
ab 33/93
abc 33/90
abc 67/88
efg 67/8
efg 8
fg 67/6
efg 67/9
defg 33/11
cd 47/3
fgh 49/2
ef 64/2
jkl 65/1
jklm 53/1
abcd00081/0
bcdefgh00066/0
abcde00075/0
hi00042/0
fgh00048/0
Na2CO3
2 EC=
4 EC=
6 EC=
8 EC=
10 EC=
bcdefg 75
fgh 60
I 667/36
jk 667/6
k0
cdefg 10
kl 5/5
lm 833/3
n 667/.
n0
abc 67/83
d 33/15
d0
D0
d0
bcdefg 33/16
a 667/84
bc 33/33
a 100
g0
fgh 37/2
p 62/0
p 0
p 0
p 0
abcdefg0007/0
ij00021/0
j 0
j 0
j 0
Control
0 EC=
a 95
a 28/16
a 100
g0
a 43/4
a00095/0
Table 3: Influence of concentration of salts solution on Germination and growth parameters in Basil (Greenhouse experiments)
Leaf
area (cm)
Radicle dry
weight (g)
Radicle fresh
weight (g)
Plumule dry
weight (g)
Plumule fresh
weight (g)
Leaf number
Radicle
length (cm)
Plumule
length (cm)
Salt
KCl
K2SO4
NaHCO3
Na2SO4
CaCL2
NaCL
Na2CO3
b 7/2893
c 1/989
bc 5/1825
bc 1/2077
bc 6/1896
b 4/2983
bc 5/1937
b 11/0
bc 079/0
c 042/0
bc 074/0
c 041/0
bc 071/0
c 046/0
b 27/0
bc 217/0
c 125/0
bc 2/0
c 125/0
b 27/0
c 122/0
b 74/0
c 24/0
bc 42/0
bc 53/0
c 22/0
bc 49/0
bc 38/0
b 72/2
c 22/1
c 43/1
bc 78/1
c 41/1
bc 14/2
bc 89/1
abc 489/10
e 767/6
de 078/7
cde 122/9
e 622/6
a 33/12
bcd 589/9
ab 51/7
cd 48/5
d 02/5
ab 87/7
d 99/4
bc 89/6
bc 189/7
bc 41/29
de 078/21
cd 33/19
bc 067/30
e 67/16
b 44/33
cd 22/24
Control
a 7/4367
a 24/0
a 97/0
a 25/1
a 05/4
ab 12
a9
a 3/42
Different letters refer to significant differences between treatments
1042
World Appl. Sci. J., 15 (7): 1039-1045, 2011
Table 4: Comparison of salts solution averages on growth parameters in Basil (Greenhouse experiments)
Treatment
-----------------------------------------------------------------------------------------------------------------------------------------------------------------Concentration
Salt
Kcl
K2SO4
NaHCO3
Na2SO4
CaCL2
NaCL
Na2CO3
(EC)
Radicle dry
Leaf area (cm) weight (g)
Radicle fresh
Plumule dry
Plumule fresh
weight (g)
weight (g)
weight (g)
Leaf number
Radicle
Plumule
length (cm)
length (cm)
2
abcdefg 2333
bc 133/0
bcd 28/0
ab 98/0
abcd 76/2
ab 67/11
a 3/10
abc36
6
abc 3384
bcd 132/0
bcd 31/0
abcde 75/0
ab 186/3
abc 47/10
bcdef 9/6
bcde 9/28
10
abcde 2964
defgh 063/0
cdef 22/0
bcdef 47/0
abcde 2/2
bc 33/9
ef 33/5
de 33/23
2
abcdefg 2297
b 144/0
bc 327/0
bcdef 51 /0
abcde 28/2
ab 4/11
abcde 2/8
abcd 9 /34
6
efg 670
bcdef 09/0
bc 32/0
cdef 21/0
bcdef 37/1
bc 9/8
abcde 23/8
bcde 33 /28
10
g0
h0
f0
f0
f0
e0
h0
f0
2
defg 911
efgh 043/0
def 099/0
bcdef 38 /0
cdef 67/6
bc 3/8
cdef 67/6
cdef 33 /24
6
ab 3570
cdefgh 068/0
bcdef 218/0
abcd 81/0
cdef 33/6
bc 2/9
cdef 33/6
cdef 67/25
10
cdefg 995
gh 013/0
ef 057/0
ef 12/0
def 66/0
de 73/3
gh 067/2
f8
2
abcdef 2493
bcde 112/0
bcd 29/0
bcdef 54 /0
abcde 3/2
bc 10
ab 86/9
ab 6 /36
6
abcdef 2466
bcdefg 08/0
bcde 259/0
abc 85/0
abcde 29/2
abc 93/10
abc 2/9
abc 26/35
10
bcdefg 1272
fgh 027/0
ef 051/0
def 207/0
cdef 74/0
cd 43/6
fg 57/4
e 33/18
2
abcd 3313
cdefgh 07/0
bcdef 198/0
bcdef 35/0
abcde 17/2
abc 2/10
abcde 73/7
bcde 28
6
abcdefg 2377
efgh 051/0
cdef 178/0
cdef 29/0
abcde 08/2
bc 67/9
bcdef 23/7
e 22
10
g0
h0
f0
f0
f0
e0
h0
f0
2
abcdef 2624
cdefgh 07/0
cdef 185/0
abcde 7/0
abcde 36/2
a 67/14
abcdef 33/7
abc 36
6
a 4255
bcdef 1/0
b 4/0
bcdef 48 /0
abcde 7/2
ab 12
abcdef 33/7
ab37
10
abcdefg 2071
efgh 043/0
bcd 239/0
cdef 27/0
bcdef 35/1
abc 33/10
def 6
bcde 33 /27
2
abcdefg 2390
efgh 051/0
cdef 14/0
cdef 28/0
bcde 37/1
abc 97/10
abcd 9
bcde 33/29
6
abcde 2947
efgh 044/0
cdef 121/0
cdef 24/0
cdef 67/6
bc 8/9
cdef 67/6
cde 33/24
10
fg 475
efgh 041/0
def 102/0
bcdef 61/0
abc 93/2
bc 8
ef 9/5
e 19
a 4368
a 24/0
a 97/0
a 25/1
a 05/4
ab 12
abcd 9
a 3 /42
CONTROL 0
Greenhouse Experiment: In second experiment
(growth vegetative stage test) seven salt were chosen
with three salinity levels (EC= 2, 6, 10 dS/m) and distilled
water as control (Table 3 and 4). Salinity treatment was
applied when plant had four leaves. In this experiment
plumule fresh weight, radicle fresh weight and leaf area,
radicle dry weight, plumule dry weight, leaf number,
radicle and plumule length were measured at the end of
vegetative stage. Result showed that vegetative growth
reduced with increasing salinity level and the most
negative effect related to Na2SO4 and CaCl2. Also the
lowest reduction observed in control (distilled water).
At the measurement of 35th day of plant emergence,
growth of basil showed the significant reduction in saline
soil compare to control. Water salin induce the reduction
of seed water absorption and influence of growth
vegetative stage and consequently all processes related
with the use of nutrients reserves and development of
embryos. Ramin (2006) showed that basil can be classified
as moderate tolerant to salt stress during seed
germination and seedling emergence [13]. Attia (2011)
showed that salt restricted the whole of plant biomass
deposition rate by diminishing leaf number and leaf
expansion, as well as photosynthetic activity estimation
per unit leaf area [11]. The root and shoot lengths are the
most important parameters for salt stress because roots
have in direct connection to soil and absorb water and
shoot supply it to other plant tissue. For this reason, root
and shoot length show different sensitive response
in plants at salt stress conditions. Salt stress
inhibited the seedling growth (rootand shoot length,
fresh root and shoot weight) but root length was more
affected than shoots length (Table 3 and 4). Inhibition of
plant growth by salinity may be due to the inhibitory of
effect ions. The reduction in root and shoot development
may be due to toxic effects of the NaCl, as well as
unbalanced nutrient uptake by the seedlings. It may be
due to the ability of the root system to control of ions
entrance to the shoot in the presence of NaCl to plant
survival [34]. High salinity may inhibit the root and shoot
elongation due to slowing down the water uptake by the
plant [35]. Neumann (1995) indicated that salinity can
rapidly inhibit the root growth and its capacity to water
uptake and essential mineral nutrition from soil [36].
Our results also confirm the findings of Hussain and
Rehman [37].
1043
World Appl. Sci. J., 15 (7): 1039-1045, 2011
CONCLUSIONS
8.
Our results indicated that germination and growth
vegetative stage of basil were inhibited by increasing salt
concentration. Salt stress declined the germination and
also delayed the emergence of seeds. It is also assumed
that in addition to toxic effects ofcertain ions, higher
concentration of salt reduced the water potential in the
medium which make a reduction in water absorptionby
germinating of seeds and reduced germination. It showed
that in germination reduction is related to salinity that
induced disturbance of metabolic process leading to
increase in phenolic compounds [38].
9.
10.
11.
ACKNOWLEDGEMENTS
We would like to thank the Experts and faculty
members for support and assistance. Also the authors
wish to thank Shahid Chamran University of Ahvaz for
supporting this work.
12.
13.
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