> REPLACE THIS LINE WITH YOUR PAPER IDENTIFICATION NUMBER (DOUBLE-CLICK HERE TO EDIT) < 1 Designing a smart device-enabled Ultrasound probe for bone fracture and soft tissue healing. # Abeysuriya, U.H., Karunarathna, S.D., Premadasa, K.M.G.P. Department of Electrical Electronic and Telecommunication Engineering General Sir John Kotelawala Defence University # uvinduab@gmail.com Abstract— Muscle pains, soft tissue inflations and bone fractures can be considered as some of the most common injuries one could face in their day-to-day life. Though such injuries may not be life threatening, they could significant effect one’s quality of life. Therefore, minimizing time taken for healing such injuries is very important. Among several different methods available, lowintensity pulsed ultrasound (LIPUS) frequency of 1.5 MHz applied at an intensity of 30mW/cm2 has been found to accelerate bone healing, similarly varying frequency range of 0.75 MHz to 3 MHz at an intensity of 0.5 W/cm2 to 3 W/cm2, have been reported to relieve pain and accelerate soft tissue healing. Even though LIPUS has many benefits, it has not been widely used mainly because LIPUS machines are available only in hospitals and even the portable devices available in the market are large and less user friendly. Therefore, in this study we aimed to develop a LIPUS/ultrasound prototype which is portable, user-friendly, less expensive and mobile-device compatible. This prototype was developed as a mobile accessory with plug and play capability to harness the processing power of mobile devices and the increasing popularity of smart mobile devices among public. The device contains a transducer which is powered by an input voltage signal through an oscillator circuit of 3.5 MHz. It has been tested using a phantom to check whether it operates in expected frequency and intensity range. Results were compared with similar previous studies. A mobile app was also developed using Android Studio to operate the prototype. Therefore, in this study we have successfully shown that a mobile LIPUS/ultrasound prototype could be developed as a mobile accessory. However, this prototype needs to be miniaturized, built into a probe similar to the replica we designed and human trials carried out before the device is ready for public use. We believe that medical devices developed as mobile accessories are more attractive to people and will also help popularizing beneficial treatment procedures such as LIPUS which could make people’s lives better. Index Terms—Ultrasound, LIPUS, Oscillator, Bone fracture, Therapeutic I. INTRODUCTION M uscle pains, soft tissue inflations and bone fractures can be considered as some of the most common injuries that one could face in their day-to-day life. According to health sector reports, diseases of the musculoskeletal system and connective tissue stand among the top 10 leading causes of hospitalization[1]–[3]. When we consider about the people who don’t attend any medical facility for treatments , the total number of patients should be higher than what is reported[2]. Millions of bone fractures occur worldwide annually[4], after treatments 5% - 10% of those may end up as delayed healing or non-unions[4], thus improved treatment methods to enhance the fracture healing are important to make sure the speedy recovery as well as to increase the quality of life of the patients. Methods of enhancing fracture repair can be categorized in to two, physical stimulation therapies and biological therapies which can be further divided based on the method of delivery[5]. Among several physical stimulation therapies, Low-intensity pulsed ultrasound (LIPUS) is one of the latest methods to accelerate fracture healing by using an ultrasound frequency of 1.5 MHz with a pulse width of 200 µs and repetition frequency of 1 kHz, and an intensity of 30mW/cm2[4], [6]–[9]. LIPUS is a treatment procedure which would be carried out for 20 minutes per day for several days depending on the fracture conditions[9], [10]. There are two main treatment methods used in orthopedics to treat muscular disorders, Pharmacological treatment which use medicine and drugs in order to achieve healing[11], and Nonpharmacological treatments[11]. In non-pharmacological treatments, ultrasound treatment which is called as “Therapeutic Ultrasound”, has become one of the most commonly used treatments for wide variety of soft tissue injuries[11]–[13]. With a varying frequency range of 0.75 MHz to 3 MHz and intensity varying between 0.5 W/cm2 to 3 W/cm2, inducible acoustic vibrations are delivered to the site. Therapeutic ultrasound achieve deeper penetration with lower frequencies[14]. Therapeutic Ultrasound is performed in three > REPLACE THIS LINE WITH YOUR PAPER IDENTIFICATION NUMBER (DOUBLE-CLICK HERE TO EDIT) < different methods which differ according to the treatment site and condition of injury[13]. Direct method, underwater bath to treat small joints in degassed water and water bag method which uses water bag as the coupling medium instead of gel. There are two modes of the treatment which we use to treat different conditions. Pulse mode is one of those two modes which is used to treat an injury in acute conditions where thermal effect of ultrasound is not that important[14]. In Continuous mode mainly focuses on thermal effect of ultrasound to treat sub-acute and chronic stage injuries[12], [13]. As the use of smartphones is increasing rapidly all over the world and its’ ability to perform multiple functions, many have paid their attention to use the power of smart phone hardware to develop medical devices and accessories[15]–[17]. Therefore, designing a smart device enabled LIPUS probe for bone fracture and soft-tissue healing could also make ultrasound treatments more user friendly and widely used. II. BONE FRACTURE AND SOFT TISSUE INJURY HEALING A. Physiological background of bone and fracture healing The fracture healing process of a bone involves four steps: inflammation, soft callus formation, hard callus formation, and bone remodeling[4]. The inflammatory phase, also called fracture hematoma formation, is the first stage of healing that occurs immediately after the injury [6]. According to Kaloty. R et al, approximately 48 hours after the injury, blood vessels torn by the fracture release blood. This blood starts to clot and forms a fracture hematoma. Because of the disruption of blood flow to the bone, some bone cells around the fracture die[6]. This inflammatory stage ends approximately one week after the fracture. Following inflammation is the genesis of a soft or fibro cartilaginous callus. Angiogenesis promotes the delivery of osteogenic cells and fibroblasts to the site of injury, resulting in the formation of an initial “procallus” [4]. The fibroblasts secrete collagen to temporarily connect the broken ends of the bone, and the osteogenic cells differentiate in the avascular environment into chondroblasts. The chondroblasts subsequently deposit fibrocartilage, which converts the procallus into the characteristic soft callus in the process of fracture healing [18]. Fracture healing continues with the evolution of the soft callus into a hard, bony callus. This process is initiated by the differentiation of osteogenic cells into osteoblasts in the wellvascularized bone tissue. The osteoblasts initiate intramembranous ossification, replacing the soft callus with a trabeculae network of bone connecting the developing and necrotic bone fragments[4]. Bone remodeling is the final phase of fracture healing. Osteoclasts continue to remove necrotic bony tissue to 2 accommodate space for the newly formed bone. Simultaneously, osteoblasts replace the trabeculae bone with compact bone through endochondral ossification [4].. B. Physiological background of soft-tissue healing Soft tissue healing often refers to healing of stressed and strained muscular tissues due to various reasons. Myofascial pain or muscle pain is defined as pain that originates from myofascial trigger points in skeletal muscle[11]. It is prevalent in regional musculoskeletal pain syndromes, either alone or in combination with other pain generators[19]. Myofascial pain has a high prevalence among individuals with regional pain complaints[20]. The prevalence varies from 21% of patients seen in a general orthopedic clinic, to 30% of general medical clinic [21]. During the healing process of the muscle, it undergoes through three phases[22], [23]. Those can be known as destruction phase, repair phase and remodeling phase. Destruction phase is characterized by the rupture of the myofibers, the formation of a hematoma between the ruptured muscle stumps, and the inflammatory cell reactions[22]. The repair phase, is consisting of the phagocytosis of the necrotized tissue, the regeneration of the myofibers, and the concomitant production of a connective tissue scar, as well as the capillary ingrowth into the injured area[22], [23]. The last phase, the remodeling phase, is a period during which the maturation of the regenerated myofibers, the contraction and reorganization of the scar tissue, and the recovery of the functional capacity of the muscle occur. And also repair and remodeling phases are closely associated or overlapping[23]. III. ULTRASOUND IN MEDICINE Ultrasound is generally the sound, with the frequencies above the audible range 20Hz – 20000Hz (20kHz). As it is a mechanical energy, it requires a medium to travel. Ultrasound cannot propagate through vacuum like electromagnetic waves. The frequency ranges of ultrasound, which is used in medical field is in 1MHz – 20MHz range. The frequency and the intensity of the ultrasound wave is decided as to the requirement and the penetration power needed. Most common use of the ultrasound in medical field is diagnosing. Ultrasound scanning plays a major role in diagnostic instrument which is vastly use in pregnancy imaging, body organ observations etc. Apart from imaging, lately experiments have shown that there are useful effects of ultrasound in soft-tissue pain healing (Therapeutic ultrasound) and non-union bone fracture healing (LIPUS). A. Therapeutic Ultrasound Therapeutic ultrasound is a treatment which is commonly used in Orthopedic treatments. It is used in achieving pain relief, soft > REPLACE THIS LINE WITH YOUR PAPER IDENTIFICATION NUMBER (DOUBLE-CLICK HERE TO EDIT) < tissue inflammation, soft tissue addition breakdown, tissue repairing and phonophoresis. This treatment is applied in two modes Pulsed mode and Continuous mode which is decided by the physiotherapist as to the conditions of the injury, patient and the location of the injury. Pulsed mode is used for acute conditions, where it use intensity varying from 0.8 W/cm2 to 3W/cm2 and frequency of 1MHz or 3MHz, which will be decided according to the depth of the injury trigger point[24]. In continuous mode, where it is used in subacute and chronic stage injuries, and uses intensities varying from 0.8 W/cm2 to 2 W/cm2. Thermal effect plays a major role in this mode. Therapeutic ultra sound can be applied by using three methods, Direct method, Under water bath method and Water bag method. Although this treatment gives advantage of increasing permeability of cells, providing a micro massage and thermal effect, it cannot be applied on vascular conditions, radiotherapy sites, pregnancy and tumors etc. And also it has potential hazards like burns and cavitation. B. LIPUS LIPUS treatment is one of the recent advancements of using Ultrasound in medical applications. It has shown enhancement in fracture healing in number of studies. This LIPUS treatment is a treatment procedure which may carry out form 10 days to 60 days which rely on the condition of the fracture. It is a 20minute treatment procedure which has to carryout daily. LIPUS refers to a pulsed ultrasound which has 1MHz sine waves repeating at 1 kHz, average intensity 30mW/cm², pulse width 200 μs[4], [25]. 3 and can be downloaded from different internet stores and markets. A mobile device could readily communicate health records, laboratory outcomes and radio-diagnostic interpretations, thus reducing waiting time and establishing an environmentally friendly workplace by reducing the quantity of paper used. Also, Mobile technology advancement creates fresh possibilities for improving health and streamlining communication between physicians and patients. Mobile medical applications (apps) can encourage wellness programs, remind patients to take drugs or plan appointments, and even monitor vital factors[26]. Medical apps are widely used by doctors and patients for medical purposes. According to one survey study, most of the apps that were requested were about medical books and references, evidence-based medicine and up-to-date literature[27]. At present, the use of technical devices among medical professionals’ ranges from 45% to 85%. Nearly threefourths of physicians said smartphones were needed to access the internet during their job, and medical learners noted that smartphones were precious instructional resources[28]. Younger physicians tended to own a smartphone and use it to interact with each other. In addition, mobile devices have often been used as a reference and information management tool among junior experts[27]. Mobile phones play a major role in spreading innovative instruments such as medical applications (apps). Using smartphones in an internal medicine clinic showed simple and quick access to suitable data. Further analyses of the use of applications and services would assist to enhance this area and comprehend healthcare professionals' requirements[29]. As an example, the use of smart phones and smart apps in Sonography/ Ultrasound technology to diagnose and analyse diseases could be shown where, ultrasound imaging devices are operated with the use of smart devices such as mobile phone and tabs because of their high processing power. This helps to achieve fast transferring of data as well as the visuals to the doctor if he is remotely located[17]. As similar to the therapeutic ultrasound treatment, LIPUS carries similar limitations and hazards. It is therefore important to understand that currently, the healthcare industry is in a transition phase where many of the personal medical devices are replaced by sensors and accessories developed for smart phones. With increasing processing power and convenience, the rate of adoption of such technologies are very high. IV. USE OF SMART DEVICES AND SMART APPS IN HEALTHCARE Therefore, in this study we aimed to design a smart device enabled ultrasound probe for bone fracture and soft tissue healing. It does not focus on thermal effect of ultrasound, but mainly on mechanical effect. INDUSTRY Nowadays, many individuals have various portable smart devices such as personal digital assistants (PDAs), smartphones, wearable systems (e.g. intelligent watches, glasses) and tablet PCs. Smartphones were effectively incorporated into the daily routine, in particular. Mobiles' are called smartphones with internet connection and computer-like characteristics. Software programs used in these smart devices are called apps that have been developed for a specific function V. METHODOLOGY A. Development of the smart-device application. The GUI of smart-device app which is used to operate the ultrasound probe was designed initially. In the designing process, the main concern was to make the app more user friendly. Android Studio was selected as the mobile application > REPLACE THIS LINE WITH YOUR PAPER IDENTIFICATION NUMBER (DOUBLE-CLICK HERE TO EDIT) < 4 developer software. Mobile app was developed using high level language Java, which is vastly used in similar projects to develop medical purposed mobile phone applications[16]. User friendly interface with appropriate instructions at every level was an important feature to guide the user without any complications. In it, there were different interfaces in order to start the treatment, where simple knowledge about the injury was enough to select the appropriate treatment procedure. Therefore, the mobile app was developed with few selection buttons such as injury type, place of the injury, time after the injury occurred, to start and stop the treatment and to proceed or decline for the warning messages, which were easy to understand for the general user. The bone interface was developed to only contain buttons for Start, Stop and Back and a countdown timer was also included. The app was developed in such a way that a warning message appears prior to the treatment informing the patient if he/her had any heart or metal transplant or, if the patient was pregnant, he/she should have medical advices. Similarly, this message appears before the treatment for muscle started. Muscle interface was divided for two types of muscle aches, ‘Neck/Back’ and ‘Leg/Hand’. Both those interfaces were again divided as to the time from the injury occurred, less than two days from the injury (< 2 days after injury) and more than two days from injury (> 2 days after injury). B. 3D rendered design of the probe The probe was designed using Solidwork software (Dassault Systèmes, 2018 version). As it is shown in the Figure 1 below, the 3D design of the probe was approximately 10 cm long and maximum 4 cm wide. The probe head had a diameter of 3 cm. The probe contained a USB female port in order to connect with the mobile phone. And also there was a LED indicator which was to indicate when the connection with the mobile phone was completed. Figure 2: Oscillator circuit to generate signal to power ultrasound probe It was tested on breadboard by using DC power supply and oscilloscope. The output signal was acquired from oscilloscope and the observed output had the wave pattern which was expected, that had the frequency of 3.58MHz. The peak-to-peak output voltage (Vpp) had shown a lesser value than expected. It could only reach 1.6Vpp when the supplied input voltage was 9V. A value of 2.4Vpp could observe at 15V input voltage. In order to power the ultrasound transducer, it had to be above 5Vpp. D. Data collection In order to validate the device’s intensity, an appropriate setup was designed to collect data. Increase in temperature for a given duration by a given intensity was investigated to check whether the transducers function as intended. The temperature increment of a bone inside a 3.3% agar block was carried out by Ohwatashi [30]. Data collection setup was prepared as described by Ohwatashi [30] with minor modification to the protocol (Figure 3). Figure 3: Data collection setup Figure 1: Solidwork design of the probe C. Development of the Circuit The oscillator circuit was designed to generate the wave pattern which was required to power the ultrasound transducer. The dimensions of the phantom which was used to collect data was 4cm×4cm×3cm. It was decided to put the bone at 2cm from the bottom of the phantom and to keep a hole to access near the bone surface from the side. IR thermometer was selected to measure temperature as it is capable of giving accurate readings in a similar setup. Two clamps were used in order to keep the positions of transducer and thermometer unchanged during the process. The temperature of the bone surface was required to measure in every 40 seconds for 10 minutes. It was also > REPLACE THIS LINE WITH YOUR PAPER IDENTIFICATION NUMBER (DOUBLE-CLICK HERE TO EDIT) < important to keep the heights marked as H1 and H2 unchanged throughout the process. Since the power circuit of the device could not be completed, actual data collection could not be carried out as yet. However, once the data collection is completed those will be compared with the Ohwatashi’s work [30], where parameters are set at 1 MHz and 3 MHz ultrasound with 2 W/cm2. Statistical analysis of data will be done and graphs will be generated using Graph pad prism software. VI. DISCUSSION Throughout the process of developing the mobile phone app and ultrasound probe, there were several issues that had to be overcome. The issues that were to sought out during the mobile phone app development were mainly about making the GUI of the app more user friendly and the safety features of it. Circuit development was more challenging procedure than it was in developing mobile app. Those were mainly about generating the voltage signal which was required to operate ultrasound probe and about providing sufficient voltage to the probe in order to operate. To address those issues several options were tested during the circuit development process. Those issues have been discussed under this section. A. Development of mobile app During the process of mobile app development, the initially designed app contained several interfaces which were to represent bone treatment, and muscle treatments. In that, muscle treatment was divided into two interfaces, as Pulsed and Continuous. In those interfaces there were rotter knobs used, which were required to adjust the intensity. In Android Studio mobile app developing software, there was no rotter knob template. Therefore, it was required to import the rotter knob template from other external library called Beppi Library. It was decided that the initial design was too complex to operate and not that user friendly. Therefore, it was adjusted, in order to make it more user friendly. In that case the number of interfaces were increased but the number of function buttons were limited to ‘Start’, ‘Stop’ and ‘Exit / Back’. It was important to add an indicator to inform the user about the battery level of the probe. Therefore, a battery level indicator was added to the mobile app, which was not mentioned in initial GUI of the mobile app. And also as safety features, warning messages which were to be appeared before the treatments were started and ended were added. And the beeping alarm was set to indicate when the treatment was completed in order to inform any third party who is interested in the treatment procedure. This feature could be useful in clinical environment such as hospitals. And when the beeping alarm was added, it was considered about the patient’s comfort, as there might be some people who doesn’t want others to know about their medical conditions. Therefore, the alarm which was added, was not loud or sound for longer period. In the future, it is better the mobile app to have a data base, which carries the treatment data including, patient name, 5 treatment type and treatment time which would be helpful in clinical environment and for patients who are having ultrasound treatments for fracture healing and muscular disorders, in order to check the patient’s treatment history. B. Development of the circuit The oscillator circuit which was mentioned in the methodology, was tested on bread board initially with using the NPN transistor BC548. As it was a circuit which should has the ability of generating 3MHz ultrasound, a crystal oscillator with 3.58MHz frequency was used. When the oscillator tested using DC power supply and Oscilloscope, the observations supported that the circuit was capable of providing the wave pattern which was required. It was giving a similar wave pattern which was acquired using a similar device purchased online. But the peak-to-peak voltage of the output signal was very low. It was at 870mV – 920mV range when the given input was 5V, 1.2V – 1.3V at 9V input, and 2.1V – 2.2V range was shown when the input was 15V. This was the major challenge which had to face throughout the development of the circuit. Initial step which was taken to overcome this obstacle was to change the resistor values and the capacitor values, and check the output signal on the oscilloscope. But it could not provide any increase in output voltage. Then the next move was to try different transistors by replacing BC548. 2N3904 and 2N2222 were the transistors used to replace BC548. With the use of those, it was evident that there was a little increment in the output voltage. When 5V input voltage was given, the output was at 940mV – 980mV range. It was at 1.4V – 1.6V range when the input was 9V. Output peak-to-peak voltage of 2.3V – 2.4V range could be observed when the input was 15V. But it was not sufficient enough to power the ultrasound transducer. But with both 2N3904 and 2N2222, it was observed that they generate a clear wave pattern at about 6.2V input. It was decided to use 2N3904 as the replacement for BC548, as it gave the highest output voltage increment. Next step taken was to try and use an operational amplifier circuit as a voltage amplifier, in order to amplify the voltage of the output signal. LM741 op-amp was used in the process. But the effort was not successful as the output signal of the oscillator was 3.58MHz, and the op-amp couldn’t amplify a signal with that frequency. As the output voltage signal didn’t have the required peak-topeak voltage, and also the op-amp circuit couldn’t amplify the output, a transistor voltage amplifier circuit was tested to see whether the signal could amplify. 2N3904 was the transistor used in the amplifier circuit, which was the same used in oscillator circuit. After observing the output using oscilloscope, we couldn’t notice any amplification in output voltage. The output voltage had dropped further down and it was 440mV at 9V input. But we could observe a clear wave pattern at a low input voltage such as 4.5V. All in all, the development of the circuit to power the transducer was challenging as there were > REPLACE THIS LINE WITH YOUR PAPER IDENTIFICATION NUMBER (DOUBLE-CLICK HERE TO EDIT) < no specifications available for the ultrasound transducer used in the probe [12] C. A. Speed, “Therapeutic ultrasound in soft tissue lesions,” Rheumatology, vol. 40, no. 12, pp. 1331–1336, 2001. [13] J. H. Demmink, P. J. M. Helders, H. Hobæk, and C. Enwemeka, “The variation of heating depth with therapeutic ultrasound frequency in physiotherapy,” Ultrasound Med. Biol., vol. 29, no. 1, pp. 113–118, 2003. [14] A. Wright and K. A. Sluka, “Nonpharmacological Treatments for Musculoskeletal Pain,” Clin. J. Pain, vol. 17, no. 1, pp. 33–46, 2001. [15] L. Mosa, A.S.M.; Yoo, I.; Sheets, “A systematic review of healthcare applications for smartphones. BMC Med.Inf. Decision Making,” BMC Med. Inform. Decis. Mak., vol. 12, no. 67, 2012. [16] H. Graf and K. 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Yaman et al., “The use of mobile smart devices and medical apps in the family practice setting,” J. Eval. Clin. Pract., vol. 22, no. 2, pp. 290–296, 2016. [30] A. O, S. I, and K. H, “Temperature changes caused by the difference in the distance between the ultrasound transducer and bone during 1 m h z and 3 m h z continuous ultrasound : a phantom study,” pp. 1– 4, 2015. VII. CONCLUSION The aim of this research was to design a smart deviceenabled ultrasound probe for bone fracture and soft tissue healing. During the process, the mobile phone app was completed with the required features including simple GUI. In the circuit development, since several parameters needed to be tested regarding ultrasound transducer, it took longer time than expected. The transducer was not excited either with voltage amplifications or current amplifications as it was expected. Therefore, all aspects of the circuit design could not be completed. 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