Biol 523 Quiz 3 Key
October 17, 2019
Name ______________________
1. Which of the following plants has a phenotype similar to that of wus?
a. clv3-2
b. 35S::CLV3
c. UFO::CLV3
d. CLV3::CLV3
e. both b and c
2. Which of the following statements about CLV3 mRNA in 35S::CLV3 plants is true?
a. It is only present in the central zone of the shoot apical meristem.
b. It is only present in the peripheral zone of the shoot apical meristem.
c. It is expressed throughout the shoot but shows higher levels in the middle of the shoot
apical meristem.
d. It is not expressed in seedlings.
3. The flowers produced by some 35S::CLV3 transgenic plants lack _________.
a. sepals and petals
b. stamens and carpels
c. petals and stamens
d. sepals and carpels
e. none of the above
4. Which of the following statements is true?
a. The UFO promoter is regulated by CLV3 signaling.
b. CLV3 mRNA is present at lower levels in 35S::CLV3 plants as compared with wild-type
plants.
c. CLV3 mRNA is not present in 35S::CLV3 clv1-4 plants.
d. 35S::CLV3 clv1-4 plants resemble clv1-4.
5. Location of WUS mRNA in wild-type plants.
a. All cells of the peripheral zone of the shoot apical meristem.
b. All cells of the central zone of the shoot apical meristem.
c. All L1 cells of the shoot apical meristem.
d. All L3 cells of the shoot apical meristem.
e. Some L3 cells in the central zone of the shoot apical meristem called the organizing
center.
6. Location of WUS mRNA in 35S::CLV3 plants.
a. All cells of the peripheral zone of the shoot apical meristem.
b. All cells of the central zone of the shoot apical meristem.
c. Most L1 cells within the shoot apical meristem.
d. Most L2 cells of the shoot apical meristem.
e. WUS mRNA does not accumulate in 35S::CLV3 plants.
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7. True or false. Transcription from the CLV3 promoter is negatively regulated by CLV3
signaling.
8. Which of the following statements is true?
a. CLV3 acts to restrict stem cell number by activating WUS expression within the shoot apical
meristem.
b. In UFO::CLV3 transgenic plants, CLV3 signaling activated in the underlying layers of
the meristem by the transgene is able to downregulate transcription of the endogenous
CLV3 gene in the overlying cells.
c. The CLV genes act to promote stem cell identity.
d. A functional CLV3-8 protein is made in clv3-8 plants.
9. Technique used in the paper to investigate CLV3 and WUS mRNA expression.
a. Western blot
b. Northern blot
c. in situ hybridization
d. immunolocalization
10. Which of the following statements is false?
a. The expression pattern of WUS mRNA is expanded in clv3-2 mutants compared to wild type.
b. 35S::CLV3 plants have an increased number of stem cells.
c. The 35S::CLV3 phenotype requires CLV1.
d. The CLV3 promoter is subject to feedback regulation via CLV3 signaling.
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