AMITY UNIVERSITY
NOIDA
MICROBIAL QUALITY CONTROL IN FOOD AND
PHARMACEUTICAL INDUSTRIES
SHARATH KUMAR H
A0989217063
B.Sc. (H) MICROBIOLOGY
SEMESTER 5
1.
Biosafety level one, the bottom stage, applies to work with agents that typically
pose a minimal capability of danger to laboratory workers and the surroundings
e.g. E. coli, S.cerevesiae, B. subtilis etc.
2.
Do not cause diseases in healthy adults.
3.
Research with these agents is generally executed on fashionable open laboratory
benches without the use of special containment device.
4.
BSL 1 labs are not normally isolated from the overall building. Training on the
specific methods is given to the lab personnel, who're supervised by using a trained
microbiologist or scientist.
5.
Standard microbiology practices are normally enough to shield laboratory workers
and other employees inside the building. These consist of:
6.
•
Mechanical pipetting only (no mouth pipetting allowed),
•
Secure sharps dealing,
•
Avoidance of splashes or aerosols, and
•
Decontamination of all work surfaces whilst work is complete.
Decontamination of spills is carried out immediately, and all probably infectious
materials are decontaminated prior to disposal, usually by autoclaving.
7.
Standard microbiological practices additionally require interest to personal hygiene,
i.e., Hand washing and a prohibition on consuming, ingesting or smoking within the
lab.
8.
Normal laboratory personal protective equipment (PPE) is typically worn,
consisting of eye safety, gloves and a lab coat or gown.
1. Biosafety level two would cover work with agents associated with human disease, in
other words, pathogenic or infectious organisms posing a moderate risk. Examples
are the equine encephalitis viruses and HIV when performing routine diagnostic
methods or work with clinical specimens.
2. Therefore, because of their capacity to cause human diseases, terrific care is used to
save you percutaneous injury (needlesticks, cuts and other breaches of the pores and
skin), ingestion and mucous membrane exposures further to the same old
microbiological practices of BSL 1.
3. Contaminated sharps are dealt with severe warning.
4. Use of disposable syringe-needle units and suitable puncture-resistant sharps
packing containers is obligatory.
5. Direct handling of broken glassware is prohibited, and decontamination of all sharps
previous to disposal is standard practice.
6. Access to the lab is greater controlled than for BSL 1 facilities.
7. Immunocompromised, immunosuppressed and other men and women with extended
risk for contamination may be denied admittance at the discretion of the laboratory
director.
8. BSL 2 labs need to also provide the subsequent stage of boundaries, i.e., speciality
safety equipment and facilities.
9. Preferably, that is a Class II biosafety cabinet or equal containment device for work
with agents and an autoclave or different suitable technique for decontamination
inside the lab. A readily available eyewash station is needed. Selfclosing lockable
doors and biohazard caution signs also are required at all access points.
1. Yellow fever, St. Louis encephalitis and West Nile virus are examples of agents
requiring biosafety stage three practices and containment.
2. Work with these agents is strictly controlled and must be registered with all
appropriate government businesses.
3. These are indigenous or extraordinary agents which can cause critical or deadly
disease through aerosol transmission, i.e., easy inhalation of particles or droplets.
4. The pathogenicity and communicability of these agents dictates the subsequent level
of shielding methods and barriers.
5. Add to all of the BSL 2 practices and device even extra stringent get entry to manage
and decontamination of all wastes, which includes lab clothing earlier than laundering,
in the lab facility.
6. Baseline serum samples are collected from all labs and other at risk personnel as
appropriate.
7. More protective primary barriers are used in BSL 3 laboratories, including solid-front
wraparound robes, scrub suits and respirators as necessary.
8. Facility design should incorporate self-closing double-door access separated from
general building corridors.
9. The ventilation should offer ducted, directional airflow through drawing air into the
lab from easy regions and with no recirculation.
1. Agents requiring BSL four facilities and practices are extraordinarily risky and pose
an excessive risk of life-threatening disease.
2. Examples are the Ebola virus, the Lassa virus, and any agent with unknown dangers
of pathogenicity and transmission.
3. These centers provide the maximum safety and containment.
4. To the BSL three practices, add necessities for entire clothing change before entry, a
shower on exit and decontamination of all substances prior to leaving the ability.
5. The BSL 4 laboratory have to include a Class III biological safety cabinet however
may also use a Class I or II BSC in combination with a positive-pressure, air-provided
full-body suit.
6. Usually, BSL four laboratories are in separate buildings or a totally isolated region
with dedicated supply and exhaust ventilation.
7. Exhaust streams are filtered through high efficiency particulate air (HEPA) filters,
depending upon the agents used.