Safety Highlights
Lab Safety
Biosafety
Chemical Safety
Radiation Safety
Highlights
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Training Requirements
Resources
Responsibilities
Lab Safety Plan
– Dept. ME Safety Plan
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U of MN Safety Plan
Training Requirements
All ME employees
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Initial training : 2 options to satisfy
– In person, presented by EHS
1.
Lab Safety and Hazardous Waste Training
– Online
1.
2.
3.
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Intro to Research Safety
Chemical Safety
Chemical Waste Mgmt
Annual refresher training
– By DEHS (in the fall) or ME
Training Requirements
Lab members
Procedure- or lab-specific training requirements
 Animal handling
– IACUC Certification
– (SPF Training)
– (Controlled substances training)
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Human or NHP tissue exposure
– Bloodborne pathogens (Online)
– (NHP Training)
Cont.
Training Requirements
Lab members (cont.)
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Laser
– Contact Rad Safety, EHS
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Training
Set up
Working with radioactive materials
– Radioactive materials
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Radiation Safety Training
Training and Safety Resources
Dept. EHS
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DEHS: Training
http://www.dehs.umn.edu/training/
– Online training
– Registration for in person training
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DEHS: Assistance and resources
– 6-6002
Training and Safety Resources
Dept. Mechanical Engineering
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Dept. Mechanical Engineering: Safety
Web page
https://me.umn.edu/info/services/safety/index.s
html
Index from ME Safety page:
– Emergency Information and Phone Numbers
(pdf)
– Department Lab Safety Plan (pdf) (links to
appropriate resources are included in this plan)
– Annual Update Attendance Form (pdf)
– Initial Update Attendance Form (pdf)
– Teaching Lab Safety Form (pdf)
– Laboratory Audit Checklist (pdf)
Training and Safety Resources
Bioheat and Mass Transfer Lab
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Laboratory Safety Handbook (on
bookshelf)
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Chemical Waste handling and packaging instructions
Compressed gas safety information
Annual departmental training notes
Transition to online Lab Safety Handbook
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Possible excepted items saved as hardcopy, as
required by U of MN Safety Plan
Responsibilities: PI /
Supervisor
Supervisors/Principal Investigators
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The immediate supervisor of a laboratory employee is responsible for:
1) assuring that potential hazards of specific projects have been identified and addressed
before work is started;
2) ensuring there are written, laboratory-specific standard operating procedures for the
protocols carried out in the laboratory that incorporate directions about how to mitigate
the hazards of the procedures.
3) informing and training employees regarding the specific hazards in their area and in the
work they will be doing;
4) scheduling time for the employee to attend designated training sessions;
5) enforcing U of MN safety policies and safe work practices;
6) conducting periodic audits of the research space under the supervisors control;
7) reporting hazardous conditions to the college or departmental research safety officer;
8) investigate laboratory accidents and send an Accident Investigation Worksheet
(Appendix N) with recommendations to the departmental research safety officer for
review.
Performance will be measured by:
• home department's documentation of current, pertinent safety training for the supervisor
and each employee in the supervisor's group;
• home department's documentation of regular audits for laboratory space under the
control of the supervisor.
(from Dept. ME Safety Plan)
Responsibilities:
Employee
Employee
 Employees who have significant responsibility for directing
their own laboratory work are
– responsible for assuring that potential hazards of specific projects
have been identified and addressed before work is started.
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All laboratory employees however, are responsible for:
• attending safety training sessions;
• following safety guidelines applicable to the procedures
being carried out;
• assuring that required safety precautions are in place before
work is started; and
• reporting hazardous conditions as they are discovered.
(from Dept. ME Safety Plan)
U of MN Safety Plan
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Chapter 5 Working with Chemicals
Introduction
Prudent Planning
General Procedures for Working with Hazardous Chemicals
Working with Substances of High Toxicity
Working with Biohazardous and Radioactive Materials
Working with Flammable Chemicals
Working with Highly Reactive or Explosive Chemicals
Working with Compressed Gases
Chapter 6 Working with Laboratory Equipment
Introduction
Working with Water-Cooled Equipment
Working with Electrically Powered Laboratory Equipment
Working with Compressed Gases
Working with High/Low Pressures and Temperatures
Using Personal Protective, Safety, and Emergency Equipment
Emergency Procedures
Safety Plan and SOPs for
‘BMTL’
Safety Plan for ‘BMTL’
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Training plan
Each lab member be trained according to Dept. ME standards, and,
Each lab member be trained according to specific research uses
Training records
Record of lab members’ training – tracking list
Hardcopy documentation of training – in Lab Safety Handbook
(currently)
Transition to softcopy documentation via BMTL web site
Lab safety equipment
Fire extinguisher, eye wash and shower
Hazards and restrictions posted
Biohazardous culture or waste
Sharps
Radioactivity
Lab audit (internal) every 6 months
Will develop an audit procedure and tracking
Safety Plan and SOPs for
‘BMTL’ (cont.)
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Each individual should identify hazards associated
with any research procedure, then:
1.
2.
Include identification of hazards in SOP (in section on
Safety)
Include instructions for “mitigation of hazard” in SOP (in
section on Safety)
1.
2.
3.
3.
4.
Handling of materials
Labeling of materials or waste
Instructions for waste disposal
Post hazard areas, if nec.
Label any hazardous materials or waste
(See resources at DEHS and ME sites)
Biological Safety
Hazards
1. Risk of infection
2. Spread of biological agent to other
samples or cultures
3. Contamination of work areas and
equipment
4. Contamination of areas outside work
area or lab
Biological Safety Hazards:
Other species
http://www.ahc.umn.edu/rar/safety.html
 Zoonoses
 NHP
– Require training to handle tissue or blood
– Herpes Simplex B
Biological Safety Levels
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BSL 1
– Low risk: normal uninfected samples; probability
that sample contains agents pathogenic to
humans is low
– Includes uninfected samples from mouse, pig,
dog
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BSL 2
– Higher risk: samples many contain pathogenic
agents that are of“moderate potential hazard”
– Normal human samples, mouse with prion Tg
Biological Safety
– Non-infected animals are BSL1
– Human samples are BSL2
– Standard Practices BSL1
– Biosafety Level 1 (BSL-1):
– Biosafety Level 1 is suitable for work involving well-characterized
agents not known to consistently cause disease in healthy adult
humans, and of minimal potential hazard to laboratory personnel and
the environment. The laboratory is not necessarily separated from the
general traffic patterns in the building. Work is generally conducted on
open bench tops using standard microbiological practices. Special
containment equipment or facility design is neither required nor
generally used. Laboratory personnel have specific training in the
procedures conducted in the laboratory and are supervised by a
scientist with general training in microbiology or a related science.
– The following standard and special practices, safety equipment and
facilities apply to agents assigned to Biosafety Level 1:
BSL 2 in this lab
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Human cultures and tissues
Mouse cells with prion Tg
– This is considered BSL 2, since it has
been passaged through the mouse, and is
not a human prion.
Prion Tg mouse cells:
Biosafety Level 2
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“Thus, … once human prions are passaged in
mice and mouse PrPSc is produced, these prions
should be considered Biosafety Level 2 prions,
even though the human prions are Biosafety
Level 3 under most experimental conditions.
An exception to this statement is in the case of
mice expressing human or chimeric
human/mouse transgenes. These transgenic mice
produce human prions when infected with human
prions and should be treated as Biosafety Level 2
or 3 in accord with the guidelines described
above. “
From CDC
Procedures for BSL 1
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Wash hands after handling viable materials, after
glove removal, and before leaving lab
No eating, drinking in the work areas. Food stored
outside work area in “food only” storage areas.
Proper sharps handling procedures
Minimization of aerosols and splashes
Decontamination of work surfaces after use and
after spill
Waste (cultures, samples)
– Must be decontaminated before disposal (with
10% bleach, 15 min., or other decontamination
solution, or by autoclaving)
– Or, must be sealed in labeled biohazard
bag/container for removal
Procedures for BSL 1
(cont.)
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Biosafety hood not required at this
level
Lab coats recommended
Gloves optional
Eyewear, if splashes expected
Biological Safety for BSL 1 and
BSL2
 Sharps
– Disposal in labeled needle box
– Glass pipets and broken glass in labeled
pipet container
– Never recap needles or replace sharps
in packaging
– Minimize glass
– Use luer lock
Biological Safety for BSL 1 vs BSL2
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Protective clothing
– Lab coat
recommended,
gloves optional,
safety glasses (if
outside hood, with
risk of aerosol)
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Biosafety hood
– Not required
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Protective clothing
– Lab coats required and are
not worn outside of work
areas
– Gloves required
– Plus face protection (if
outside hood, with risk of
aerosol)
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Biosafety hood (and sealed
rotors)
must be used human or
other class II agents if
– Aerosols or splashes
– High concentration or
large volume of infectious
agents
Additional Procedures for BSL 2
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Specific training for handling the pathogenic
agents is required (Hepatitis B, Herpes Simplex
B)
Procedures that generated aerosols or splashes
must be performed in biosafety hood (or similar
equipment)
Routing handling of human tissue and blood
requires baseline serum and testing;
recommended: Hepatits B immunization
Any injury must be reported– Report to lab supervisor/PI
– Injury form submitted
– Visit to Boynton
Additional Procedures for BSL 2
(cont.)
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Decontamination of equipment and supplies
– Prior to washing glassware, instruments, etc., they must
be decontaminated with 10% bleach, if not stainless
steel, or with diluted decontamination solution for 15
minutes before being washed and prepared for
autoclaving.
– Contaminated glassware and instruments that are
removed from hood should be placed into dishpan for
decontamination.
– Decontamination should be done immediately after
completing the procedure.
– To prevent any toxic effects from the decontamination
solution, rinse items very well before autoclaving
Biological Safety Procedures:
Human Tissue/Cells
http://www.ibc.umn.edu/principleJan2005.html
 Use precautions specified by Bloodborne Pathogens
training
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Lab coat
Gloves
Neutralization of waste with 10% bleach before disposal
Disposal of waste
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Biohazard waste bag, sealed
Request pick up from johns098
Biohazard waste is autoclaved before disposal
– Decontaminate hood and work area with diluted bleach, or
for stainless steel, diluted decontaminant solution
Other methods for waste
collection and disposal for
BSL2
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To avoid spills and aerosols, may
collect waste from tubes and flasks by
inserting sterile pipets into vacuum
tubing set up inside hood
– This must be set up with a biohazard
waste trap: include bleach at 10% total
flask volume, before collecting the waste
Other information
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Pipet aid
– Contains a filter unit inside nozzle.
If medium is drawn into pipet aid, wetting filter
unit, air will not pass through filter
 Filter must be replaced
 Filters are stored in drawer _drawer under water
bath_ by hood
– Occasionally, the cotton plug will be
drawn into pipet aid nozzle. This can be
removed with a forceps