Cell structure and organelles A cell is the smallest part of an organism all cells consist of a membrane, cytoplasm and a nucleus. Types of typical cells : Feature Eukaryote ( true nucleus ) Plant Cell 1| Prokaryote ( no nucleus ) Animal Cell Bacteria Present but not made of cellulose 1- Cell wall Present and made of cellulose Absent 2- Nucleus Present Present Absent , Genetic material DNA spreads in cytoplasm . Plus small circular DNA called plasmids 3- Shape Regular shape because of cell wall Irregular shape due to no cell wall Different shapes : Spherical – Rod – spiral 4- Size Large Small Very small 5- Organelles Present Present Absent , except ribosomes which needed for protein synthesis a. Chloroplast Present Absent b. Mitochondria Present Present c. Vacuole Mainly one Large Permanent Many Small Temporary d. Carbohydrate storage Starch Glycogen e. Rough endoplasmic reticulum ( RER) Present Present Dr.Adel Guirguis Master degree in Genetic engineering and tissue culture \ 2| Dr.Adel Guirguis Master degree in Genetic engineering and tissue culture Part Function Cytoplasm – Contains cell organelles – Chemical reactions take place e.g. respiration and making proteins Cell Membrane Forms a barrier between the cell and its surroundings. Controls what enters and exits the cell e.g. glucose Cell wall keeps contents of cell inside ; Stops cells from bursting when they filltowith – Allows movement of simple substances enterwater and leave the cell e.g. O2 , – CO Gives andshape waterto cells 2 – Allows water and dissolved substances to pass through freely Nucleus – Nucleus : contains DNA in the form of chromosomes Functions : Controls: 1- Cell division 2- Cell development 3- Cell activities 4- The type and quantity of enzymes produced by the cytoplasm 5- Regulates the chemical changes which take place in the cell Mitochondria Release energy from food substances during aerobic respiration Folded inner membrane to allow large surface area for enzymes needed for aerobic respiration Active cells ( muscle and liver cells ) contain more mitochondria than less active one . Rough Endoplasmic Reticulum ( RER ) Chloroplast Carry ribosomes which needed for protein synthesis ( ex. Enzymes – Antibodies-some hormones like insulin ) 1- Photosynthesis 2- Starch storage Sap vacuole 1- Full of water to maintain shape and firmness of cell 2- Stores , pigments , salts and sugars 3| Dr.Adel Guirguis Master degree in Genetic engineering and tissue culture Stem cells : Are unspecialized cells ( 2n ) chromosomes , only divide by mitosis to produce daughter identical cells (2n ) chromosomes . Daughter cells then become specialized to do certain functions Examples : 1- Bone marrow that divides all the time by mitosis to produce different types of blood cells To replace old ones. Red blood cells ( live for 120 days ) White blood cells Platelets 2- Stem cells lie in lower part of skin epidermis divide to replace worn out skin cells 3- Cambium cells in between xylem and phloem in vascular plants . It divide by mitosis to form new and extra xylem and phloem tissues Specialized Cells: It have : 4| Particular job Distinct shape Loses ability to divide any further Special chemical reactions The specialization of cells to carry out a particular function in an organism is sometimes referred to as division of labor Dr.Adel Guirguis Master degree in Genetic engineering and tissue culture 5| Dr.Adel Guirguis Master degree in Genetic engineering and tissue culture Some specialized cells in human : Cell Ciliated cells Adaptations 1- The air passages of breathing system ( trachea and bronchi ) cilia move mucus that trap dust and pathogens up the nose and throat . 2- The oviducts in the female reproductive system , cilia move the egg from the ovary to the uterus . Red blood cells : It has no nucleus , so more volume to carry More haemoglobin to transport more oxygen Haemoglobin picks up oxygen to form oxyhaemoglobin Biconcave shape : gives it a high surface area Small enough to pass through capillaries Transport oxygen from lungs to tissues Muscle cells : – Muscle cells make up fibres that are able to contract to move the body – They contain a lot of mitochondria to provide the energy needed. Contract and relax – Skeletal muscles are attached to the skeleton when they contact they move bones at joints . – Smooth muscle : o in the gut for peristalsis o in arteries and veins to control diameter of lumen – Cardiac muscles in heart 6| Dr.Adel Guirguis Master degree in Genetic engineering and tissue culture Cell Adaptations Nerve cells They conduct electrical impulses which travel to and from the brain. – They are very long and their chemical reactions cause impulses to travel through their fibers. – They also have a layer of fat ( myelin sheath ) for insulation and speed up nerve impulse. Function : transmit nerve impulse – Has an acrosome containing digestive enzymes to Sperm cell dissolve the ovum’s cell membrane – Has a haploid nucleus (1n) chromosome to fuse with egg nucleus (1n) to form zygote (2n) – Contains either X or y chromosome to determine gender of baby. – Has a tail to swim towards the ovum. Function : contains 1n chromosomes = 23 chromosome – Has many mitochondria to provide energy for swimming. – Has a small streamlined shape for easy swimming . – Haploid cell (1n) Egg cell – Large in size to store food reserve inside – Immobile to settle in oviduct Function : contains 1n chromosomes from mother = 23 chromosomes 7| Dr.Adel Guirguis Master degree in Genetic engineering and tissue culture Some specialized cells in plants Cell Adaptations – Long extensions that give them a large surface area to Root hair cells : absorb water and ions from the soil. – Large surface area for cell membrane for more carrier proteins needed for active transport of ions – Vacuole with high concentration of, salts, sugars and solutes , to give, lower water potential than soil , this promotes osmosis. – Thin cell wall to allow short distance for diffusion . Function : – More mitochondria to provide, energy ( ATP ) for active Absorption of water and mineral salts Xylem vessels: transport – The xylem cells are dead , with no cytoplasm , no nucleus to form : – long hollow tubes running throughout the root, stem, and leaves. – It has thick cell walls to allow support – The cell walls are thickened and bands or spirals of a water proof material called lignin – They help to support the plant Function : – These cells allow water and ions to move from roots to the Transports water and minerals to the plant Palisade mesophyll cells rest of the plant . – Have many chloroplasts to hold a lot of chlorophyll. To absorb as much light as possible – palisade cells arranged lengthways ; allowing less cell walls to scatter light . to allow free movement of chloroplast up or down according to light intensity , also to avoid harm to chlorophyll during intense light . Function : Absorption of light energy . 8| – palisade cells close together ; to absorb as much light as possible ; Dr.Adel Guirguis Master degree in Genetic engineering and tissue culture 2.3 Size of cells and specimens Microscopes and magnification : Magnification means how many times an object enlarged . o X10 means the object is magnified 10 times . o 1 cm = 10 mm o 1mm = 1000 micrometers =103 µm o So , 1cm = 10 mm X 103 µm = 104 µm 9| Dr.Adel Guirguis Master degree in Genetic engineering and tissue culture Practice : 1- The following figure shows one pollen grain, as seen with the aid of an electron microscope. This pollen grain has been magnified 200 times. (s05/6/Q2c ) Calculate the actual size of this grain. Show your working. ...................................................................................................................................................................... ...................................................................................................................................................................... ...................................................................................................................................................................... ...................................................................................................................................................................... actual size………………[2] 10 | Dr.Adel Guirguis Master degree in Genetic engineering and tissue culture We can also use a scale line to work out magnification . 2- The following figure is a photomicrograph of some frog blood cells. ( w15/53/ Q3b) o Measure the length of the scale bar in millimetres. o length of scale bar ........................................ mm o Use the scale bar to calculate the magnification of the photomicrographs. Show your working. o Give your answer to the nearest whole number. o ...................................................................................................................................................................... ...................................................................................................................................................................... ...................................................................................................................................................................... ...................................................................................................................................................................... magnification × ...................... 11 | Dr.Adel Guirguis Master degree in Genetic engineering and tissue culture [3]