FREND Principle TM FREND Principle TM 30µL serum/plasma Sample Inlet Dissolution and mix with sample buffer upon sample addition FREND Principle TM Fluore scent Bead Conjugate zone Dissolution and mix with fluorescence beads conjugated with 2o antibody which binds to Antigen in the sample upon propagation FREND Principle TM Test zone Immobilized capture antibodies against Antigen grab conjugate immune complexes FREND Principle TM Reference zone Immobilized proteins grab positive-control fluorescent beads FREND Principle TM Waste reservoir End of the capillary propagation of sample FREND Principle TM REF Photodiode TEST
