University of San Jose- Recoletos
Senior High School Department
MICROSCOPY
I. Introduction
“Micro" refers to tiny, "scope" refers to view or look at. Microscopes are tools used to
enlarge images of small objects so as they can be studied. The compound light microscope is an
instrument containing two lenses, which magnifies, and a variety of knobs to resolve the picture.
Because it uses more than one lens, it is sometimes called the compound microscope in addition
to being referred to as being a light microscope. In this lab, we will learn about the proper use
and handling of the microscope.
II. Objectives
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Demonstrate the proper procedures used in
correctly using the compound light
microscope.
Determine the total magnification of the
microscope.
Explain how to properly handle the
microscope.

Explain the proper procedure for focusing
under low and high power using the
compound light microscope.
Identify the different parts and functions of
the different parts of a Microscope.
III. Materials
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Compound microscope
Letter "e" slides
IV. Procedures
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General Biology 1
Laboratory Activities Series of 2019
1. Get one microscope from the counter and carefully bring it to your area.
2. Ask 1 prepared e slide from your teacher.
a. View the specimen under different magnification:
x=40
X=100
Objective Used:
X=400
Objective Used:
Objective Used:
V. Guide Questions
1.
Locate the numbers on the eyepiece and the low power objective and fill in the blanks below.
Eyepiece magnification
______________
2.
Objective magnification
______________
=
Total Magnification
_____________
=
Total Magnification
_____________
Do the same for the high power objective.
Eyepiece magnification
______________
3.
X
X
Objective magnification
______________
Write out the rule for determining total magnification of a compound microscope:
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4. Why do you think it is important to place the objectives under scanner before changing to Low
or High Power objectives?
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General Biology 1
Laboratory Activities Series of 2019