Develop an experiment with the purpose of
identifying the presence of Vitamin C in
juice.
Identify if the commercial orange juice
contains Vitamin C.
Vitamin C, also known as L ascorbic acid, is a
water-soluble vitamin that is naturally present in
some foods, added to others, and available as a
dietary supplement. Humans, unlike most
animals, are unable to synthesize Vitamin C
endogenously, so it is an essential dietary
component.
Insufficient Vitamin C can lead to scurvy.
Biosynthesis
Collagen
Synthesis of
Neurotransmitt
ers
Metabolism of
Proteins
Antioxidant
Food
Dietary
Supplements
For this experiment, we will test the presence of
Vitamin C in comercial orange juices that claim to
contain it.
We will be using an indicator made out of starch and
iodine.
Iodine and Starch are able to oxidize the Vitamin C and
as result form a blue or dark color complex, that can
serve as an indicator for the presence of Vitamin C in
juice.
Materials
1.Beakers
2.Iodine
3.Starch solution
4.75ml of Water
1. Add a table spoon of the starch solution to 75 ml of water.
2. Add Iodine until it becomes a dark blue solution.
3. Add one table spoon of the indicator into two separate beakers.
4. Add one table spoon of juice to the previously prepared beakers.
5. Identify the dark color of the indicator if it persists that means the
juice contains Vitamin C.
Do comercial brand juices contain
Vitamin C?
Write your own
hypothesis.