My Procedure-Procedure Protocol (AutoRecovered)

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My Procedure
The principal ingredients are genetically modified yeast, water, and sugar. The raw silk is
produced through fermentation, much like brewing beer, except instead of the yeast turning the
sugar into alcohol, they turn it into the raw stuff of spider silk.
Glycine, Gly, G
C2H5NO2, 75 Da, Hf= −528 kJ/mol
Melting point : 451.7 degrees Farenheight
Alanine
C3H7NO2
Melting Point: 496 °F
Procedure for Fermentation of the yeast and sugar
1. Take a small beaker containing a 20 ml sample (#4 will have a total of 25 ml) of one of
the three (we use starch twice) test solutions to your work area.
2. Measure and add 0.5 g of yeast to your beaker.
3. Mark the fermentation tube’s base with a wax pencil for identification purposes (there
will be many tubes in the incubator).
4. Stir the solution gently and pour it into one fermentation tube.
5. Tip the fermentation tube so the tail of the tube completely fills with the solution.
6. Place the tube in the incubator for 15 minutes.
7. After 15 minutes, remove the tube from the incubator and use the metric scale on the tail
of the tube to measure the distance from the tip of the tail to the level of the solution.
Take a small beaker containing
a 20 ml sample (#4 will have a
total of 25 ml) of one of the
three (we use starch twice) test
solutions to your work area.
Measure and add 0.5 g of yeast
to your beaker.
Mark the fermentation tube’s
base with a wax pencil for
identification purposes (there
will be many tubes in the
incubator).
Place the tube in the incubator
for 15 minutes.
Tip the fermentation tube so
the tail of the tube completely
fills with the solution.
Stir the solution gently and
pour it into one fermentation
tube.
After 15 minutes, remove the
tube from the incubator and
use the metric scale on the tail
of the tube to measure the
distance from the tip of the tail
to the level of the solution.
Procedure for Combining these 2 Proteins
1.
2.
3.
4.
Melt them both down
Combine in a Fire Safe Container (Fire Safety Procedures below)
Allow fermentation
Use the Syringe and Electro spinner to create a Fabric
5. Once the Fabric has been created I will test the strength of the Fabric with air pressure to
simulate a gun shot
6. I will test the other 3 fabrics ( Polyester, cotton, and silk)
7. Repeat steps 5-7 , 3 times.
Melt them both down
I will test the other 3
fabrics ( Polyester, cotton,
and silk)
Combine in a Fire Safe
Container (Fire Safety
Procedures below)
Once the Fabric has been
created I will test the
strength of the Fabric
with air pressure to
simulate a gun shot
Allow fermentation
Use the Syringe and
Electro spinner to create
a Fabric
Repeat steps 5-7 , 3 times.
Fire Procedures
-
Use engineering control measures including ventilation and exhaust system, temperature
control, stirring facility, appropriate container
-
Wear appropriate personal protective equipment
-
Understand and follow safe operating procedures
o including procedures for use of engineering control and personal protective
equipment and for waste disposal
-
Understand contingency measures, and in case of accident follow emergency procedures
o including procedures for firefighting, evacuation, first aid, treatment of spillage
Procedure for Kobalt 12-Volt Car Air Inflator
- Place the inflator on a flat surface, near the item that will be inflated.
- Insert the power cord (H or O) into an appropriate power source (12 Volt DC or 120 Volt AC).
- Connect the air hose (G) quick connect valve adaptor to the item you are inflating, and push the
valve lock lever down to secure the connection.
- Set the power selector switch (B) to the desired position (12 Volt or 120 Volt). The digital
pressure gauge (C) will show the current pressure of the item you are inflating.
- Press the scroll buttons (J) to set the desired PSI (between 3 and 120). When the digital
pressure gauge (C) is displaying the target PSI, the readout will blink three times at the desired
preset level, and then change back to the current PSI level of the item you are inflating.
- Press the on button (K) to begin inflating the item. The inflator will automatically stop when
the item reaches the preset PSI level.
Disconnect the power cord (H or O) from the power source.
- Place the inflator on a flat surface, near the item that will be inflated.
- Insert the power cord (H or O) into an appropriate power source (12
Volt DC or 120 Volt AC).
- Connect the air hose (G) quick connect valve adaptor to the item you
are inflating, and push the valve lock lever down to secure the
connection.
- Set the power selector switch (B) to the desired position (12 Volt or
120 Volt). The digital pressure gauge (C) will show the current pressure
of the item you are inflating.
- Press the scroll buttons (J) to set the desired PSI (between 3 and 120).
When the digital pressure gauge (C) is displaying the target PSI, the
readout will blink three times at the desired preset level, and then
change back to the current PSI level of the item you are inflating.
- Press the on button (K) to begin inflating the item. The
inflator will automatically stop when the item reaches
the preset PSI level.
Disconnect the power cord (H or O) from the
power source
Procedure for using Electro Spinner
1. Connect the output cable to the high voltage power supply. Attach a crocodile clip to the other
end of the output cable. If the cable is too stiff, a short wire may be used as bridging connection
between the output cable and the electrospinning needle.
WARNING: The electrical insulation of the bridging wire may be too thin to fully insulate the high
voltage passing through the wire from the environment thus it should be kept as short as possible and
away from other surfaces
2. The electrospinning setup assembly shall be located in a fume hood while the high voltage power
supply should be placed outside with the high voltage output cable running into the fume hood
deck.
TIP: Have a black board behind the electrospinning setup to facilitate visualization of the spinning jet.
3. If the frame of the fume hood is made out of metal, connect an earth wire using crocodile clip
from the frame to the earth terminal on the high voltage power supply.
4. Place the syringe pump on an adjustable jack.
5. Cut out a piece of aluminum foil that can cover the surface of the jack. Use office clips to hold
the foil to the jack surface.
6. Connect an earth wire (wire from the earth terminal of the high voltage power supply) from the
aluminum foil using a crocodile clip.
7. Rest the jack on its side with the aluminum foil in the direction facing the syringe pump.
8. Prepare about 4 ml of solution one day in advance.
9. Measure appropriate mass of the polymer powder and pour into a glass container.
10. Measure appropriate volume of water and pour into the glass container.
11. Use magnetic stirring bar to mix the solution on a magnetic stirrer.
WARNING: Ensure the high voltage power supply is switched off and the voltage is set to zero
12. Switch on the syringe pump and set the feed rate to 1 ml/h.
13. Transfer the solution into the syringe.
14. Screw in the needle and expel any air trapped in the syringe until a drop of solution is formed on
the needle tip. Use paper wipes to clean off the solution droplet.
Note: By pointing the syringe tip skyward and squeezing out the air.
15. Place the syringe on the syringe pump and clip the crocodile clip which is connected to the high
voltage power supply to the needle.
16. Switch on the syringe pump.
17. When a drop of solution emerges from the tip of the needle, switch on the high voltage power
supply. Turn up the voltage until the solution drop starts to contort and a jet is observed to form at
the tip. Generally, this voltage is about 6 kV and above depending on the solution.
18. Lower the fumehood sash to reduce exposure to aerosol and fibers.
19. Wait for a couple of minutes. White deposits should be seen on the aluminum foil.
NOTE: Extra precautions are required when spinning hazardous solution.
20.
21.
22.
23.
Switch on the blower of the fumehood.
Turn the voltage down to zero and switch off high voltage power supply.
Raise the fumehood sash.
Use the earthed wire (from the collector or connected to the fumehood) to touch the needle and
the body of the syringe pump to ensure they are properly discharged.
24. Stop the syringe pump.
25. Remove the aluminum foil and examine the collected fiber under an optical microscope.
26. Repeat the procedure to continue spinning as necessary.
those children that are born lie dead on the naked earth unpitied, spreading contagion of death;
and gray-haired mothers and wives ->-:A?0-:-;<)6,)<<0-)4
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