Assay kits
January 2013
Discover more at abcam.com
Contents
Catalog contents
Welcome to Abcam
About us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
More than just antibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Comprehensive information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
100% supoort . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
Fast global delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
Abreviews® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
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Abbreviations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
Product list
Product . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8
Apoptosis and Cell Viability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8
Cellular Damage and Oxidative Stress . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10
Cell signaling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
Cellular staining . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16
Epigenetics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18
Metabolism . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26
MitoSciences . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32
Molecular Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34
Contact details
Abcam’s Terms and Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35
Contact details . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .36
Local offices and distributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37
Resources: www.abcam.com
Welcome to Abcam
About us
Founded in 1998 by Dr. Jonathan Milner, our mission was
to produce and sell the best antibodies in the world with
the most comprehensive, honest and up-to-date
datasheets. Several years on and thousands of products
later, we’re more than just antibodies yet these values are
just as relevant today as they ever were. We still aim to
provide the latest and most reliable products supported by
expert technical support and fast delivery, all so we can
help support scientists in their quest for discovery.
Welcome to Abcam
More than just antibodies
Hundreds of new products are added to our catalog each
month including a growing range of non-antibody products:
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Biochemicals
Biological dyes and thermo-reversible gels
Cell viability, cell proliferation and senescence kits
EasyLink antibody conjugation kits
ELISA, ELIPAIR, ELISPOT and FLUOROSPOT kits
Epigenetic kits
IHC kits and reagents
Metabolic kits
Proteins and peptides
Western blot reagents
Comprehensive information
Our products are accompanied by a wealth of technical data:
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Comprehensive datasheets
Customer reviews (Abreviews)
Protocols and troubleshooting tips
Frequently asked questions
We have a policy of honesty and so we share everything
that we know about our products, as soon as we know it
– it’s all on the datasheet.
Scientific support: www.abcam.com/technical
1
100% support
We’re committed to providing the highest levels of support
to our customers, which is why our technical teams are
scientists from a wide range of research backgrounds,
experienced in protein detection.
As part of our Abpromise, we guarantee high product
quality and expert support including:
Welcome to Abcam
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stated on the datasheet
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Abreviews® - independent customer reviews
Our Abreviews system allows customers to feedback
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information with all of our customers on our datasheets,
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This up-to-date information provides useful data about new
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For further information visit: www.abcam.com/Abreviews
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Resources: www.abcam.com
Abbreviations
Application
Key
Application
A
Acetyl CoA
ADP
AG
AKT
AP sites
ATP
B
BCA
BrdU
C
cAMP
cGMP
CETP
CFSE
Absorbance/plate reader
Acetyl Coenzyme A
Adenosine diphosphate
Agarose gel
Protein kinase B
Apurinic/ apyrimidic sites
Adenosine triphosphate
Beta counter
Bicinchoninic acid
Bromodeoxyuridine
Colorimetric
Cyclic Adenosine monophosphate
Cyclic Guanosine monophosphate
Cholesterylester transfer protein
5(6)-Carboxyfluorescein
N-hydroxysuccinimidyl ester
Chromatin immunoprecipitation
Cytosine guanine sites
ATP synthase subunit alpha
Cyanine 3
Cyanine 5
2’,7’-Dichlorofluorescein diacetate
Double stranded DNA
Dimethyl sulfoxide
Dipstick reader
Green Fluorescent Protein/
Enhanced GFP
Enzyme-linked immunosorbent
assay
Excitation/ Emission wavelength
Fluorometric
Actin filament
Fluorescein Isothiocyanate
Flow Cytometry
Fluorescence Microscopy
Glyceraldehyde 3 phosphate
dehydrogenase
Glutathione reduced state/
Glutathione oxidized state
Glutathione transferase
Guanosine triphosphate
Histochemistry
High density lipoprotein
High-throughput
IF
IHC-Fr/IHC-P
Immunofluorescence
Immunohistochemistry on frozen
sections / paraffin-embedded section
C-Jun N-terminal kinase
kDalton
Luminometer
Lactate dehydrogenase
Low density lipoprotein
Multidrug resistance
Myeloperoxidase
Methylation specific PCR
Nicotinamide adenine
dinucleotide oxidized/ reduced
Nicotinamide adenine dinucleotide
phosphate oxidized/ reduced
N-acetylneuraminic acid
Near infrared
Nanometer
Optical density
Oxidative Phosphorylation
Polymerase chain reaction
Pyruvate dehydrogenase
Pyruvate dehydrogenase subunit
E1 alpha
Phycoerythrin
Phosphatidylinositol 3-kinase
Pyruvate kinase
Phospholipid transfer protein
radiation counter
(scintillation counter)
Relative fluorescence units
Real time PCR
Flat-bed scanner
Thin layer chromatography
Tissue culture
Very low density lipoprotein
Western Blot
ChIP
CpG
CV
Cy3
Cy5
DCFDA
dsDNA
DMSO
DR
GFP/EGFP
ELISA
Ex/Em
F
F-actin
FITC
Flow Cyt
FM
GAPDH
GSH/GSSG
GST
GTP
H
HDL
HT/HTP
Scientific support: www.abcam.com/technical
JNK
kD
L
LDH
LDL
MDR
MPO
MS-PCR
NAD/NADH
NADP/NADPH
NANA
NIR
nm
OD
OXPHOS
PCR
PDH
PDH E1
PE
PI3-kinase
PK
PLTP
RD
RFU
RT-PCR
SC
TLC
TC
VLDL
WB
Abbreviations
Key
3
Products
Apoptosis and cell viability
Apoptosis, a specific form of programmed cell death (PCD), is
essential to ensure normal development and homeostasis in all
multi-cellular organisms. It counterbalances cell proliferation and
differentiation pathways by ensuring the removal of excess and
unwanted cells.
Apoptosis and cell viability
Apoptosis is characterized by morphological changes such as
plasma membrane blebbing and chromatin condensation in the
nucleus. At the molecular level activation of caspases sets in to
motion proteolytic degradation that leads to irreversible cell death.
Download our apoptosis card at
www.abcam.com/apoptosis-pathway-card
Annexin binding assays
Annexin V-based assays are one of the most common method for apoptosis detection due to its high affinity for
exposed phosphatidylserine (PS) on the cell surface. Annexin V is generally covalently linked to fluorescent
labels such FITC or PE for visualization of apoptotic cells using fluorescence microscopy or flow cytometry.
Annexin XII-based assays differ from annexin V assays in that the former are reversible. This reversibility
enables the detection of transient PS exposure, a mechanism associated with normal physiological processes
(such as vesicle release or embryonic development) as well as with rescuable apoptosis cell death events.
Annexin XII is conjugated to a fluorescent probe to allow visualization using fluorescence microscopy or flow
cytometry.
• Detect apoptosis in only 10 minutes
• Detection by Flow Cytometry or Fluorescence Microscopy
4
Resources: www.abcam.com/apoptosiskits
Annexin binding assays
Product
name
Kinetic Apoptosis Kits
Product
code
N/A
Flow Cyt
Flow Cyt
Flow Cyt
Flow Cyt
Flow Cyt
Flow Cyt
Flow Cyt
Flow Cyt
Flow Cyt
Flow Cyt
Flow Cyt
Flow Cyt
Flow Cyt
Flow Cyt
Flow Cyt
Flow Cyt
Flow Cyt
Flow Cyt
Flow Cyt
FM
Flow Cyt/FM
ab14084
ab14193
ab14190
ab14165
ab14142
ab14144
ab14143
ab14150
ab14147
ab14153
ab14152
ab14085
ab140865
ab14082
ab14155
ab39674
ab14154
ab14159
ab14158
ab129816
ab129817
ab14083
Apoptosis and cell viability
10X Annexin V Binding Buffer
Annexin V Unlabeled Apoptosis Reagent
Annexin V-Biotin Apoptosis Detection Kit
Annexin V-Biotin Apoptosis Detection Reagent
Annexin V-Cy3 Apoptosis Detection Kit
Annexin V-Cy3 Apoptosis Detection Kit with SYTOX
Annexin V-Cy3 Apoptosis Detection Reagent
Annexin V-Cy5 Apoptosis Detection Kit
Annexin V-Cy5 Apoptosis Detection Reagent
Annexin V-EGFP Apoptosis Detection Kit
Annexin V-EGFP Apoptosis Detection Reagent
Annexin V-FITC Apoptosis Detection Kit
Annexin V-FITC Apoptosis Detection Kit with SYTOX
X Annexin V-FITC Apoptosis Detection Reagent
Annexin V-PE Apoptosis Detection Kit
Annexin V-PE Apoptosis Detection Kit with SYTOX
Annexin V-PE Apoptosis Detection Reagent
Annexin V-PE-Cy5 Apoptosis Detection
Annexin V-PE-Cy5 Apoptosis Detection Reagent
Kinetic Apoptosis Kit (Flow Cytometry) - Annexin XII Kinetic Apoptosis Kit (Microscopy) - Annexin XII Propidium Iodide
Detection
P
hi rod
gh u
lig ct
ht
Kinetic Apoptosis Kits contain an annexin
XII probe that allows the detection of both
transient and permanent phosphatidylserine
(PS) exposure in order to quantify cell
death as it is taking place.
• No washing required – you won’t lose
any precious cells
• Minimal background fluorescence –
no more struggling with FACS or
filter compensation
• Suitable for live-cell imaging – follow the
changes on a single cell basis
You can choose between detection by Flow
cytometry or Fluorescence Microscopy.
Abcam’s Kinetic Apoptosis Kits contain pSIVA™, an annexin
XII probe that attaches to exposed PS but detaches if PS
is flipped back.
Caspases assay kits
The caspase family of highly conserved cysteine-proteases is essential for apoptosis. Initially synthesized as
inactive pro-caspases, caspases are rapidly proteolytically cleaved and subsequently activated by granzyme B,
death receptors or apoptosome stimuli. Generally, caspases can be divided into two categories: initiator caspases
(these include caspases-1, 2, 4, 5, 8, 9, 10, 11, and 12) and effector caspases (these are caspases-3, 6 and 7).
Abcam offers kits to measure individual caspase activity and to quantify multiple caspase activity simultaneously.
Also available are inhibitor drug screening kits that allow for the effective screening of caspase inhibitors.
• Colorimetric/ fluorometric detection
• Results in only 2 hours
Resources: www.abcam.com/apoptosiskits
5
Apoptosis and cell viability
Caspases assay kits
Product
name
Sample
type
Caspase 1 Assay Kit (Fluorometric)
Caspase 1 Assay Kit (Colorimetric)
Caspase 1 Inhibitor Drug Detection Kit
Caspase 2 Assay Kit (Fluorometric)
Caspase 2 Assay Kit (Colorimetric)
Caspase 2 (active) FITC Staining Kit
Caspase 2 Inhibitor Drug Detection Kit
Caspase 3 Assay Kit (Fluorometric)
Caspase 3 Assay Kit (Colorimetric)
Caspase 3 (active) FITC Staining Kit
Caspase 3 (active) Red Staining Kit
Caspase 3 Inhibitor Drug Detection Kit
Caspase 4 Assay Kit (Fluorometric)
Caspase 4 Assay Kit (Colorimetric)
Caspase 4 Inhibitor Drug Detection Kit
Caspase 5 Assay Kit (Fluorometric)
Caspase 5 Assay Kit (Colorimetric)
Caspase 5 Inhibitor Drug Detection Kit
Caspase 6 Assay Kit (Fluorometric)
Caspase 6 Assay Kit (Colorimetric)
Caspase 6 Inhibitor Drug Detection Kit
Caspase 7 Inhibitor Drug Detection Kit
Caspase 8 Assay Kit (Fluorometric)
Caspase 8 Assay Kit (Colorimetric)
Caspase 8 (active) FITC Staining Kit
Caspase 8 (active) Red Staining Kit
Caspase 8 Inhibitor Drug Detection Kit
Caspase 9 Assay Kit (Fluorometric)
Caspase 9 Assay Kit (Colorimetric)
Caspase 9 (active) FITC Staining Kit
Caspase 9 (active) Red Staining Kit
Caspase 9 Inhibitor Drug Detection Kit
Caspase 10 Assay Kit (Fluorometric)
Caspase 10 Assay Kit (Colorimetric)
Caspase 10 Inhibitor Drug Detection Kit
Caspase 12 Assay Kit (Fluorometric)
pan Caspase (active) FITC Staining Kit
pan Caspase (active) Red Staining Kit
Caspase Family Substrate Kit II Plus (Fluorometric)
Caspase Family Substrate Kit II Plus (Colorimetric)
Generic Caspase Activity Assay (Fluorometric - Green)
Generic Caspase Activity Assay (Fluorometric - Red)
Caspase Cascade Screening Kit
Cell lysate
Cell lysate
Caspase inhibitor-treated cells
Cell lysate
Cell lysate
Adherent cells, suspension cells
Caspase inhibitor-treated cells
Cell lysate
Cell lysate
Adherent cells, suspension cells
Adherent cells, suspension cells
Caspase inhibitor-treated cells
Cell lysate
Cell lysate
Caspase inhibitor-treated cells
Cell lysate
Cell lysate
Caspase inhibitor-treated cells
Cell lysate
Cell lysate
Caspase inhibitor-treated cells
Caspase inhibitor-treated cells
Cell lysate
Cell lysate
adherent cells, suspension cells
adherent cells, suspension cells
Caspase inhibitor-treated cells
Cell lysate
Cell lysate
Adherent cells, suspension cells
Adherent cells, suspension cells
Caspase inhibitor-treated cells
Cell lysate
Cell lysate
Caspase inhibitor-treated cells
Cell lysate
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Detection
F
C
F
F
C
Flow Cyt/FM/F
F
F
C
Flow Cyt/FM/F
Flow Cyt/FM/F
F
F
C
F
F
C
F
F
C
F
F
F
C
Flow Cyt/FM/F
Flow Cyt/FM/F
F
F
C
Flow Cyt/FM/F
Flow Cyt/FM/F
F
F
C
F
F
Flow Cyt/FM/F
Flow Cyt/FM/F
F
C
Flow Cyt
Flow Cyt
Flow Cyt
Product
code
ab39412
ab39470
ab102489
ab39794
ab39830
ab65612
ab102490
ab39383
ab39401
ab65613
ab65617
ab102491
ab65658
ab65659
ab102492
ab65660
ab65661
ab102493
ab39707
ab39709
ab102494
ab102495
ab39534
ab39700
ab65614
ab65618
ab102496
ab65607
ab65608
ab65615
ab65619
ab102497
ab65662
ab65663
ab102498
ab65664
ab65611
ab65616
ab102487
ab102488
ab112130
ab112131
ab102499
Calpain and cathepsin assay kits
The importance of caspases in triggering apoptosis is well established. However, inhibition of caspases only
results in a delay in apoptosis and not a complete block. Non-caspase proteases, such as calpains and cathepsins
are thought to play role in this pathway which has lead to an increased focus on their role in apoptosis. We offer
a variety of kits to quickly measure calpain and cathepsin activity.
• Results in only 2 hours
• Convenient 96 well plate format
Calpain and cathepsin assays
6
Product
name
Sample
type
Calpain Activity Assay Kit
Cathepsin B Activity Assay Kit (Fluorometric)
Cell lysate
Cell lysate
Detection
Product
code
F
F
ab65308
ab65300
Resources: www.abcam.com/apoptosiskits
Calpain and cathepsin assays (continued)
Product
name
Sample
type
Cathepsin D Activity Assay Kit (Fluorometric)
Cathepsin G Activity Assay Kit (Colorimetric)
Cathepsin H Activity Assay Kit (Fluorometric)
Cathepsin K Activity Assay Kit (Fluorometric)
Cathepsin L Activity Assay Kit (Fluorometric)
Cathepsin S Activity Assay Kit (Fluorometric)
Cell lysate
Cell lysate
Cell lysate
Cell lysate
Cell lysate
Cell lysate
Detection
Product
code
F
C
F
F
F
F
Mitochondrial Transmembrane Potential (Δψm)
ab65302
ab126780
ab65305
ab65303
ab65306
ab65307
Mitochondrial Transmembrane Potential (Δψm) is an important parameter of mitochondrial function and is used
as an indicator of cell death. The collapse of the Δψm coincides with the opening of the mitochondrial permeability
transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream
events in the apoptotic cascade. We have a number of relevant assays that allow quick detection of ΔψM.
Mitochondrial Transmembrane Potential assays
Sample
type
Detection
JC-10 Mitochondrial Membrane Potential Assay
Kit (Flow Cytometry)
JC-10 Mitochondrial Membrane Potential Assay Kit
(Microplate)
Mitochondrial Transmembrane Potential Apoptosis
Detection Kit
NIR Mitochondrial Membrane Potential Assay Kit
(Flow Cytometry)
NIR Mitochondrial Membrane Potential Assay Kit (Microplate)
Orange Mitochondrial Membrane Potential Assay Kit
(Flow Cytometry)
Orange Mitochondrial Membrane Potential Assay Kit
(Microplate)
TMRE—Mitochondrial Membrane Potential Assay Kit
Adherent cells, suspension cells
Flow Cyt
ab112133
Adherent cells, suspension cells
F
ab112134
Adherent cells, suspension cells
Flow Cyt/ FM
ab65310
Adherent cells, suspension cells
Flow Cyt
ab112149
Adherent cells, suspension cells
Adherent cells, suspension cells
F
Flow Cyt
ab112150
ab138898
Adherent cells, suspension cells
F
ab138899
Adherent cells, suspension cells
C/F
ab113852
JC-10 Mitochondrial Membrane Potential
Assay Kit (Flow Cytometry) (ab112133)
JC-10 is a lipophilic dye that selectively enters into mitochondria,
where it changes its color from green to orange as the membrane
potential increases. In healthy cells, JC-10 concentrates in the
mitochondrial matrix where it forms orange fluorescent
aggregates (Em = 570 nm). In apoptotic and necrotic cells, on the
other hand, the decrease in mitochondrial membrane potential
due to the opening of the pores leads to the disgregation of
JC-10 into green fluorescent monomers (Em = 520nm).
• Simple visualization of live/dead cells
• Higher solubility in water than JC-1
Healthy cell
↑ΔψM
Product
code
P
hi rod
gh u
lig ct
ht
Apoptotic/necrotic cell
↓ΔψM
JC-10 aggregates
JC-10 monomeric
Color orange
Color green
Em = 570 nm
FACS channel= FL2
Apoptosis and cell viability
Product
name
Em = 520 nm
FACS channel = FL1
DNA Ladder and Fragmentation
As a consequence of cells entering apoptosis, endonucleases are activated, which in turn cleave chromatin into
fragments of around 200 base pairs. These so called ladders can be visualized by agarose gel electrophoresis.
Alternatively, DNA fragmentation and especially the presence of DNA nicks can be shown by terminal
deoxynucleotidyl transferase (TUNEL) assays, where a fluorescently labeled nucleotide is incorporated at the
end of the fragmented DNA. Choose a kit for your preferred detection technique: DNA ladder for DNA
electrophoresis, or TUNEL assay for flow cytometry or fluorescence microscopy.
• DNA ladder kits – visualization of apoptotic cells or tissues in DNA electrophoresis
• TUNEL assay kits – detection of apoptosis by flow cytometry or microscopy
Resources: www.abcam.com/apoptosiskits
: MitoSciences product
7
DNA Ladder and Fragmentation
Product
name
Sample
type
Apoptotic DNA Ladder Isolation Kit
Apoptotic DNA Ladder Detection Kit
Apoptotic DNA Ladder Detection Kit (high sensitivity)
In Situ Direct DNA Fragmentation Assay Kit (TUNEL assay)
In Situ BrdU-Red Fragmentation Assay Kit (TUNEL assay)
Apoptotic cells and tissue
Apoptotic cells
DNA from apoptotic tissue
Adherent cells, suspension cells
Tissue, adherent cells
Detection
Product
code
AG
AG
Flow Cyt/ FM
Flow Cyt
ab65627
ab66090
ab66093
ab66108
ab66110
Detection
Product
code
Flow Cyt/ FM
Flow Cyt/ FM
F/FM
WB
A
Flow Cyt/ FM
Flow Cyt/ FM
ab133073
ab133124
ab133075
ab65311
ab110172
ab133078
ab112151
Detection
Product
code
Contains Actionomycin D:50ul
Camptothecin: 1ml
Cycloheximide (28%): 1ml
Dexamethasone: 1ml
Etoposide: 100ul
ab102480
Other apoptosis kits
Product
name
Sample
type
7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit
Apoptosis Membrane Blebbing Assay Kit
Autophagy/Cytotoxicity Dual Staining Kit
Cytochrome c Releasing Apoptosis Assay Kit
Cytochrome c Protein Quantity Microplate Assay Kit
Lysosome/Cytotoxicity Dual Staining Kit
Nuclear Apoptosis Assay Kit
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells
Cell lysate
Cell culture extracts, Tissue
Adherent cells, suspension cells
Adherent cells, suspension cells
Apoptosis and cell viability
Apoptosis inducers
Product
name
Sample
type
Apoptosis Inducer Kit (Ready-to-use)
Live cells - most common cell culture types
Cell viability, proliferation and senescence
Determining the viability of cultured cells is an essential process prior to any cell-based study: only a healthy
culture will show reliable and reproducible results. Moreover, quantification of cellular proliferation can help to
determine optimal culture conditions, to establish whether certain mutations show a phenotype that influences
viability, as well as the cytotoxic effect that certain drugs have on the overall cell population.
Abcam offers a range of products that can be used to assess cell viability, proliferation activity, cytotoxicity and
cell death. As sometimes it can be difficult to know which parameter is the most suitable for your experiment, we
have summarized the different types of assays available for the different determinations:
Which assay is most suitable for your experiment?
Which assay is appropriate
for your experiment?
Annexin assays
ATP & ADP determination assays
Caspase assays
Cell cycle assays
Cell proliferation assays
Cell viability assays
DNA fragmentation (TUNEL) assays
Glutathione (GSH/GSSG) determination assays
LDH-cytotoxicity assays
Live-dead cell assays
Mitochondrial transmembrane potential (Δψm) assays
Non-caspase proteases assays
Senescence assays
Proliferation
Viability
Cytotoxicity
P
P
P
P
P
P
Apoptosis
P
P
P
P
P
P
P
P
P
P
P
P
P
P
• Available in multiple formats to fit your laboratory instrumentation: flow cytometry, plate reader or microscope
• Results in only 20 minutes (e.g., ab65470)
• Reliable measurement of cell proliferation from only 100 cells/well (e.g., ab102501)
8
Resources: www.abcam.com/apoptosiskits
Cell viability, proliferation and senescence
Sample
type
ADP Colorimetric/Fluorometric Assay Kit
Cell lysate, cell culture medium,
urine, plasma, serum
Cell lysate, cell culture medium,
urine, plasma, serum
Cell lysate, cell culture medium,
urine, plasma, serum
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Cell lysate, urine, plasma, serum
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells , tissue
ADP/ATP Ratio Assay Kit (Bioluminescent)
ATP Colorimetric/Fluorometric Assay Kit
Cell Cycle Assay Kit (Fluorometric - Green)
Cell Cycle Assay Kit (Fluorometric - Red)
Cell Cytotoxicity Assay Kit (Colorimetric)
Cell Cytotoxicity Assay Kit (Fluorometric)
Cell Viability Assay Kit
Cell Viability Assay Kit (Fluorometric - Blue)
Cell Viability Assay Kit (Fluorometric - Dual Green/Red)
Cell Viability Assay Kit (Fluorometric - Green)
Cell Viability Assay Kit (Fluorometric - Near InfraRed)
Cell Proliferation Fluorescence Assay Kit
LDH-Cytotoxicity Assay Kit
LDH-Cytotoxicity Assay Kit II
Live-Dead Cell Staining Kit
Quick Cell Proliferation Assay Kit
Quick Cell Proliferation Assay Kit II
Senescence Detection Kit
Detection
Product
code
C/F
ab83359
L/B
ab65313
C/F
ab83355
Flow Cyt
Flow Cyt
C
F
L/B
Flow Cyt/FM/F
Flow Cyt/FM/F
Flow Cyt/FM/F
Flow Cyt/FM/F
FM
C
C
FM
C
C
IHC-Fr/IHC-P
ab112116
ab112117
ab112118
ab112119
ab65314
ab112120
ab112121
ab112122
ab112123
ab102501
ab65391
ab65393
ab65470
ab65473
ab65475
ab65351
Apoptosis and cell viability
Product
name
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Cell Viability Assay Kit
(Fluorometric – Green) (ab112122)
A range of 0 – 5000 CHO-K1 cells/well were seeded overnight, and
grown cells were incubated with the Green-dye Solution for 1 hour
at 37°C.
• Accurate measurement as fluorescence intensity is linear to the
number of cells
• High sensitivity – detects as little as 30 cells/well
Glutathione assay kits
Glutathione is the principal intracellular low-molecular-weight thiol and its central role in the cellular redox
homeostasis in mammalian cells is well recognized. Glutathione oxidation is a major contributor to cell apoptosis
mediated by oxidants; diminished levels of glutathione have been observed in the early stages of apoptosis.
Our kits can be used to measure glutathione levels in a broad range of samples ranging from live cells, lysates,
serum to tissue culture supernatant.
• Results in only one hour
• Accurate quantification of key enzymes: glutathione peroxidase, glutathione reductase and
glutathione-S-transferase.
Glutathione assay kits
Product
name
Sample
type
Glutathione Detection Kit (Fluorometric)
Glutathione Peroxidase Assay Kit
Glutathione Reductase (GR) Assay Kit
GSH/GSSG Ratio Detection Assay Kit (Fluorometric - Green)
GST Activity Assay Kit (Fluorometric)
GST Activity Assay Kit (Colorimetric)
Intracellular GSH Assay Kit
Cell lysate, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
Cell lysate, plasma, urine
Cell lysate, urine, plasma, serum
Cell lystaes, urine, plasma, serum
Adherent cells, suspension cells
Resources: www.abcam.com/apoptosiskits
: MitoSciences product
Detection
Product
code
F
A
A
F
F
C/A
Flow Cyt
ab65322
ab102530
ab83461
ab138881
ab65325
ab65326
ab112132
9
Cellular damage and Oxidative stress
Cellular damage and oxidative stress
Every day, the cells of our body suffer damage caused by exogenous factors such as physical damage, UV
light, ionizing radiation and endogenous factors arising from oxidative stress. Cellular repair mechanisms are
essential to reverse and limit the damage caused and the resultant effect on replication or cell viability.
Download our OXPHOS pathway at
card at www.abcam.com/OXPHOS
Abcam offers a variety of kits to accurately measure a whole range of metabolites involved in oxidative stress and
cellular damage pathways such as nitric oxide (NO) and reactive oxygen species (ROS) levels as well as activity
of antioxidant proteins such as catalase, superoxide dismutase or xanthine oxidase.
• Easy to scale up or down depending on the number of tests: suitable for HTP
• Colorimetric or fluorometric detection available to suit your instrument requirements
• Results in less than one hour
Cell damage kits
10
Product
name
Sample
type
7-AAD/CFSE Cell-Mediated Cytoxicity Assay Kit
Aconitase Assay Kit
Aconitase Enzyme Activity Microplate Assay Kit
ADP Assay Kit (Colorimetric/Fluorometric)
ADP/ATP Ratio Assay Kit (Bioluminescent)
ATP Assay Kit (Colorimetric/Fluorometric)
Ascorbic Acid Assay Kit
Ascorbic Acid Assay Kit (Biological Samples)
Catalase Assay Kit
Ethanol Assay Kit
Glutathione Detection Kit (Fluorometric)
Glutathione Peroxidase Assay Kit
Glutathione Reductase (GR) Assay Kit
Glutamate Assay Kit
GSH/GSSG Ratio Detection Assay Kit (Fluorometric - Green)
GST Activity Assay Kit (Fluorometric)
GST Activity Assay Kit (Colorimetric)
Intracellular GSH Assay Kit
Hydrogen Peroxide Assay Kit
NAD/NADH Assay Kit
NADP/NADPH Assay Kit
Nitric Oxide Assay Kit (Fluorometric)
Nitric Oxide Assay Kit (Colorimetric)
Adherent cells, suspension cells
TC supernatants, urine, plasma, serum
Cell culture extracts, Tissue
Cell lysate, culture medium, urine, plasma, serum
Cell lysate, culture medium, urine, plasma, serum
Cell lysate, culture medium, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
Cell lysate, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
Cell lysate, plasma, urine
Cell lysate, urine, plasma, serum
Cell lysate, urine, plasma, serum
Adherent cells, suspension cells
Cell lysate, plasma, serum, urine
Cell lysate, urine, plasma, serum
Cell lysate ,urine, plasma, serum
Cell lysate, urine, plasma, serum
Cell lysate, urine, plasma, serum
Detection
Product
code
Flow Cyt/ FM
C
A
A/C/F
L/B
A/C/F
A
A
A/F
A/F
F
A
A
A
F
F
C/A
Flow Cyt
A/F
A
A
F
A
ab133073
ab83459
ab109712
ab83359
ab65313
ab83355
ab65346
ab65656
ab83464
ab65343
ab65322
ab102530
ab83461
ab83389
ab138881
ab65325
ab65326
ab112132
ab102500
ab65348
ab65349
ab65327
ab65328
Resources: www.abcam.com/celldamagekits
Cell damage kits (continued)
Product
name
Sample
type
Superoxide Dismutase Activity Assay Kit (Colorimetric)
Thioredoxin (TrxR) Reductase Assay Kit
Total Antioxidant Capacity Assay Kit
Uric Acid Assay Kit
Xanthine Oxidase Assay Kit
Cell lysate, urine, plasma, serum
Cell lysate, urine, plasma, serum
Cell lysate, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
Detection
Product
code
A
A
A
A/F
A/F
ab65354
ab83463
ab65329
ab65344
ab102522
Detection
Product
code
A/C/F
L/B
A/C/F
H
Flow Cyt
Flow Cyt
L/B
Flow Cyt/FM/F
Flow Cyt/FM/F
Flow Cyt/FM/F
Flow Cyt/FM/F
FM
A
A
FM
A
A
H
ab83359
ab65313
ab83355
ab102534
ab112116
ab112117
ab65314
ab112120
ab112121
ab112122
ab112123
ab102501
ab65391
ab65393
ab128687
ab65470
ab65473
ab65475
ab65351
Detection
Product
code
Flow Cyt
Flow Cyt
A
A
IF
IF
Flow Cyt/FM
C
FM
ab112116
ab112117
ab65473
ab65475
ab133071
ab133068
ab14083
ab65353
ab133076
Cell viability, proliferation and senescence
Sample
type
ADP Assay Kit (Colorimetric/Fluorometric)
ADP/ATP Ratio Assay Kit (Bioluminescent)
ATP Assay Kit (Colorimetric/Fluorometric)
Beta galactosidase Detection kit
Cell Cycle Assay Kit (Fluorometric - Green)
Cell Cycle Assay Kit (Fluorometric - Red)
Cell Viability Assay Kit (Bioluminescent)
Cell Viability Assay Kit (Fluorometric - Blue)
Cell Viability Assay Kit (Fluorometric - Dual Green/Red)
Cell Viability Assay Kit (Fluorometric - Green)
Cell Viability Assay Kit (Fluorometric - Near InfraRed)
Cell Proliferation Fluorescence Assay Kit
LDH-Cytotoxicity Assay Kit
LDH-Cytotoxicity Assay Kit II
Cell Lysis Solution for ab65393
Live-Dead Cell Staining Kit
Quick Cell Proliferation Assay Kit
Quick Cell Proliferation Assay Kit II
Senescence Detection Kit
Cell lysate, culture medium, urine, plasma, serum
Cell lysate, culture medium, urine, plasma, serum
Cell lysate, culture medium, urine, plasma, serum
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Cell lysate, urine, plasma, serum
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, tissue
Cellular damage and oxidative stress
Product
name
DNA damage and cell cycle arrest kits
Product
name
Sample
type
Cell Cycle Assay Kit (Fluorometric - Green)
Cell Cycle Assay Kit (Fluorometric - Red)
Quick Cell Proliferation Assay Kit
Quick Cell Proliferation Assay Kit II
p53 (pS392) + Total p53 Dual Staining Kit
p53 Translocation Assay Kit (Cell-based)
Propidium Iodide
DNA Damage - AP sites - Assay Kit (Colorimetric)
DNA Damage - AP sites - Assay Kit (Cell-based)
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells
Adherent cells
Purified damaged DNA
Adherent cells, suspension cells
Oxidative stress assay kits
Product
name
Sample
type
Aconitase Assay Kit
Ascorbic Acid Assay Kit
Ascorbic Acid Assay Kit (Biological Samples)
Catalase Assay Kit
DCFDA - Cellular Reactive Oxygen Species
Detection Assay Kit
Glutathione Detection Assay Kit
Glutathione Peroxidase Assay Kit
Glutathione Reductase (GR) Assay Kit
GSH/GSSG Ratio Detection Assay Kit (Fluorometric - Green)
GST Activity Assay Kit (Colorimetric)
GST Activity Assay Kit (Fluorometric)
TC supernatant, plasma, serum, urine
TC supernatant, plasma, serum, urine
TC supernatant, plasma, serum, urine
TC supernatant, plasma, serum, urine
Adherent cells, suspension cells
A
A
A
A/F
C/F
ab83459
ab65346
ab65656
ab83464
ab113851
Cell lysate, plasma, serum, urine
TC supernatant, plasma, serum, urine
TC supernatant, plasma, serum, urine
Cell lysate, plasma, urine
TC supernatant, plasma, serum, urine
TC supernatant, plasma, serum, urine
F
A
A
F
C/A
F
ab65322
ab102530
ab83461
ab138881
ab65326
ab65325
Resources: www.abcam.com/celldamagekits
: MitoSciences product
Detection
Product
code
11
Cellular damage and oxidative stress
Oxidative stress assay kits (continued)
Product
name
Sample
type
Hydrogen Peroxidase Assay Kit (Cell-based)
Hydrogen Peroxide Assay Kit
Hydrogen Peroxide Assay Kit (Fluorometric - Near Infrared)
Intracellular GSH Assay Kit
Lipid Hydroxyperoxide (LPO) Assay Kit
Lipid Peroxidation (MDA) Assay Kit
Myeloperoxidase (MPO) Assay Kit (Colorimetric)
Myeloperoxidase (MPO) Assay Kit (Fluorometric)
NAD/NADH Assay Kit
NADP/NADPH Assay Kit
Nitric Oxide Assay Kit (Colorimetric)
Nitric Oxide Assay Kit (Fluorometric)
Superoxide Dismutase Activity Assay Kit (Colorimetric)
Thioredoxin (TrxR) Reductase Assay Kit
Total Antioxidant Capacity Assay Kit
Xanthine Oxidase Assay Kit
Adherent cells, suspension cells
TC supernatant, plasma, serum, urine
Cell culture extracts
Adherent cells, suspension cells
Cell culture extracts, tissue extracts, plasma
Cell culture extracts, plasma, tissue
TC supernatant, plasma, serum, urine
TC supernatant, plasma, serum, urine
Cell lysate, plasma, serum, urine
Cell lysate, plasma, serum, urine
Cell lysate, plasma, serum, urine
Cell lysate, plasma, serum, urine
Cell lysate, plasma, serum, urine
TC supernatant, plasma, serum, urine
Cell lysate, plasma, serum, urine
TC supernatant, plasma, serum, urine
Nitric Oxide Assay Kit (Colorimetric) (ab65328)
Detection
Product
code
FM
A/F
F
F
A
A/F
A
F
A
A
A
A
A
A
A
A/F
ab138874
ab102500
ab138886
ab112132
ab133085
ab118970
ab105136
ab111749
ab65348
ab65349
ab65328
ab65327
ab65354
ab83463
ab65329
ab102522
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This kit allows you to determine total nitrate/nitrite levels
in an easy three step process.
Step one
Convert nitrate to nitrite utilizing nitrate reductase
Step two
Addition of Griess Reagent to convert nitrite to a deep
purple azo compound
Step three
Measurement in microplate reader
Example of a standard calibration curve of nitrate (nitrite) following product protocol and using
nitrite standard provided in Abcam’s Nitric Oxide Assay Kit (Colorimetric) (ab65328).
Results in
less than
1 hour
12
Resources: www.abcam.com/celldamagekits
Cell signaling
Each cell in a multicellular organism needs to be able to respond to extracellular signals produced by other
cells, whether they are in the vicinity or in other organs. Cell signaling is a complex mechanism that requires
multiple players: extracellular signal molecules need to carry the signal to the target cell, where specific
receptors will bind and respond to the signal. The intracellular cascade originated by the receptors will
transduce, amplify and spread the signal eliciting a physiological response, which can be short term
(metabolism alterations) or long term (gene activations).
Abcam offers a complete range of kits to measure and detect signaling molecules in essential pathways such
as PI3 kinases, small GTPases and second messengers.
• Our kits can be used for a variety of sample types
• High sensitivity and broad detection range
General kinase assay kits
Product
name
Sample
type
Universal Kinase Assay Kit (Fluorometric)
Cell lysate, purified protein
Detection
Product
code
F
ab138879
Detection
Product
code
A/F
A/F
A
A
A
F
A
A/F
A
ab65334
ab65333
ab102517
ab102532
ab83426
ab107923
ab102529
ab65620
ab65335
Detection
Product
code
WB
ab65786
Detection
Product
code
A
A
A
A
A
A
A
ab83360
ab102509
ab83372
ab102510
ab83366
ab102506
ab102507
Detection
Product
code
WB
ab65784
Product
name
Sample
type
Glucose and Sucrose Assay Kit
Glucose Assay Kit
Glucose Detection Kit
Glucose Dehydrogenase Assay Kit (Colorimetric)
Glucose 6 Phosphate Assay Kit
Glucose 6 Phosphate Assay Kit (High Sensitivity)
Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric)
Glycogen Assay Kit
Maltose and Glucose Assay Kit
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
Cell signaling
AMPK, Insulin and Leptin signaling kits
GSK3/WNT signaling kits
Product
name
Sample
type
AKT Activity Assay Kit
Cell lysate, tissue extracts
Ion and metals signaling
Product
name
Sample
type
Ammonia Assay Kit
Ammonia Assay Kit - Modified Berthelot - (Colorimetric)
Chloride Assay Kit
Cobalt Quantification Kit
Iron Assay Kit
Magnesium Detection Kit
Zinc Quantification Kit
Cell lysate, culture medium, urine, plasma, serum
Cell lysate, culture medium, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
Cell lysate, plasma and serum, urine
Cell lysate, culture medium, urine, plasma, serum
Cell lysate, culture medium, urine, plasma, serum
JNK/SAP signaling kits
Product
name
Sample
type
JNK Activity Screening Kit
Cell lysate, tissue extracts
Resources: www.abcam.com/cellsignalingkits
13
Lipid signaling kits
Product
name
Sample
type
PAF Acetylhydrolase Assay Kit
PAF Acetylhydrolase Inhibitor Screening Assay Kit
cytosolic Phospholipase A2 Assay Kit
secretory Phospholipase A2 Assay Kit
PPAR (alpha, delta, gamma) Transcription Factor Assay Kit
PPAR alpha Transcription Factor Assay Kit
PPAR delta Transcription Factor Assay Kit
PPAR gamma Transcription Factor Assay Kit
Sphingomyelin Assay Kit (Colorimetric)
Sphingomyelin Assay Kit (Fluorometric)
Sphingomyelinase Assay Kit (Fluorometric)
Sphingomyelinase Assay Kit (Fluorometric - indirect)
SREBP-2 Translocation Assay Kit (Cell-based)
Cell lysate, plasma, tissue homogenate
Pharmarcological compounds
Cell culture extracts, tissue extracts
Plasma
Adherent cells, suspension cells, nuclear extracts
Adherent cells, suspension cells, nuclear extracts
Adherent cells, suspension cells, nuclear extracts
Adherent cells, suspension cells, nuclear extracts
Plasma, serum
Cell culture extracts, whole blood
Cell culture extracts, whole blood
Cell lysate, saliva, serum, tissue extracts, urine
Adherent cells
Detection
Product
code
A
A
A
A
A
A
A
A
A
F
F
F
IF
ab133088
ab133091
ab133090
ab133089
ab133113
ab133107
ab133106
ab133101
ab133118
ab138877
ab138876
ab133108
ab133114
Detection
Product
code
A
IF
ab133074
ab133123
IF
ab133121
Detection
Product
code
WB
TLC
ab65786
ab128503
Detection
Product
code
A
F
A/C/F
L/B
A/C/F
A
F
F
A
F
F
ab83367
ab83370
ab83359
ab65313
ab83355
ab83369
ab83371
ab138887
ab65622
ab102508
ab112155
Detection
Product
code
A
A
F
A
A
L
F
F
F
ab65355
ab65356
ab65327
ab65328
ab102505
ab112114
ab112115
ab112128
ab112129
MAPK signaling kits
Product
name
Sample
type
Cell signaling
ATF2 (pT69/pT71) Transcription Factor Assay Kit
Adherent cells, suspension cells, nuclear extracts
Adherent cells
ERK/MAPK (pT202/pY204) Translocation
Assay Kit (Cell-based)
p38 MAPK (pT180/pY182) Translocation Assay Kit (Cell-based) Adherent cells
PI3 Kinase/AKT/mTOR signaling kits
Product
name
Sample
type
AKT Activity Assay Kit
PI3 Kinase Assay Kit
Cell lysate, tissue extracts
Purified protein
Phosphate signaling kits
Product
name
Sample
type
Acid Phosphatase Assay Kit (Colorimetric)
Acid Phosphatase Assay Kit (Fluorometric)
ADP Assay Kit (Colorimetric/Fluorometric)
ADP/ATP Ratio Assay Kit (Bioluminescent)
ATP Assay Kit (Colorimetric/Fluorometric)
Alkaline Phosphatase Assay Kit (Colorimetric)
Alkaline Phosphatase Assay Kit (Fluorometric)
Alkaline Phosphatase Assay Kit (Fluorometric - Near Infrared)
Phosphate Assay Kit (Colorimetric)
Phosphate Assay Kit (Fluorometric)
Pyrophosphate Assay Kit (Fluorometric)
Cell lysate, urine, plasma, serum
Cell lysate, urine, plasma, serum
Cell lysate, culture medium, urine, plasma, serum
Cell lysate, culture medium, urine, plasma, serum
Cell lysate, culture medium, urine, plasma, serum
Cell lysate, urine, plasma, serum
Cell lysate, urine, plasma, serum
Cell culture extracts, TC supernatant
Cel lysate, urine, plasma, serum
Cel lysate, urine, plasma, serum
Adherent cells, suspension cells, cell culture extracts
Second messenger kits
14
Product
name
Sample
type
cAMP Direct Immunoassay Kit
cGMP Direct Immunoassay Kit
Nitric Oxide Assay Kit (Fluorometric)
Nitric Oxide Assay Kit (Colorimetric)
Calcium Detection Kit (Colorimetric)
Calcium Assay Kit (Luminometric)
Calcium Quantification Kit (Fluorometric)
Fluo-8 Medium removal Calcium Assay Kit
Fluo-8 No Wash Calcium Assay Kit
Cell lysate, urine, plasma, serum
Cell lysate, urine, plasma, serum
Cell lysate, urine, plasma, serum
Cell lysate, urine, plasma, serum
Cell lysate, culture medium, urine, plasma, serum
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Resources: www.abcam.com/cellsignalingkits
Second messenger kits (continued)
Product
name
Sample
type
Rhod-4 Medium Removal No Wash Calcium Assay Kit
Rhod-4 No Wash Calcium Assay Kit
Adherent cells, suspension cells
Adherent cells, suspension cells
Detection
Product
code
F
F
ab112156
ab112157
Detection
Product
code
WB
WB
WB
WB
ab139586
ab128506
ab128505
ab128504
Detection
Product
code
F
F
ab107921
ab112152
F
ab112153
F
ab112154
Small GTPases signaling kits
Product
name
Sample
type
Rac Activation Kit
Ral Activation Kit
Rap Activation Kit
Ras activation Kit
Cell culture extracts
Cell culture extracts
Cell culture extracts
Cell culture extracts
Ubiquitin Proteasome pathway kits
Sample
type
Proteasome Activity Assay Kit
Proteasome Activity Assay Kit (Fluorometric - Green)
Cell lysate
Cell culture extracts, plasma, whole blood,
other biological fluids
Cell culture extracts, plasma, whole blood,
other biological fluids
Adherent cells, suspension cells
Proteasome Activity Assay Kit (Fluorometric - Red)
Proteasome 20S Activity Assay Kit (Fluorometric)
Pyrophosphate Assay Kit (Fluorometric) (ab112155)
Cell signaling
Product
name
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Pyrophosphates (PPi) are produced by a number of biochemical
reactions, such as ATP hydrolysis, DNA and RNA polymerization or
cyclic AMP formation. Abcam’s Pyrophosphate Assay Kit has a unique
proprietary fluorogenic sensor to measure pyrophosphate concentration
in one easy and quick step.
• Direct reading: no need for enzyme-coupling reactions;
just add sensor and measure
• Results in only 30 minutes
< 1 µM of pyrophosphate can be detected in just 10 minutes.
Resources: www.abcam.com/cellsignalingkits
15
Cellular staining
The visualization of a protein of interest in a
specific sub-cellular structure through
fluorescence microscopy allows researchers to
study the protein in its dynamic context and to
understand how it can interact with the rest of
the cell. Abcam offers a variety of kits and
labels to visualize and detect sub-cellular
structures such as actin filaments, lysosomes,
mitochondria and nuclei. Used in conjunction
with our fluorescently labeled antibodies, these
kits are the ideal tool for co-localization studies
in order to determine where the protein of
interest is positioned.
Cellular staining
• Simple to use: just add the dye, incubate and analyze
• Compatible with most common fluorescent microscope filters
• Versatile: choose the most suitable color for your experiment from up to 4 colors
F-actin staining kits
Product
name
Sample
type
F-actin Staining Kit - Blue Fluorescence
Fixed tissue sections, fixed cells or
cell-free experiments
Fixed tissue sections, fixed cells or
cell-free experiments
Fixed tissue sections, fixed cells or
cell-free experiments
Fixed tissue sections, fixed cells or
cell-free experiments
F-actin Staining Kit - Green Fluorescence
F-actin Staining Kit - Orange Fluorescence
F-actin Staining Kit - Red Fluorescence
Ex/Em
Color
Product
code
350/450nm
ab112124
500/520nm
ab112125
550/575nm
ab112126
594/610nm
ab112127
Lysosomal staining kits
Product
name
Sample
type
Ex/Em
Color
Product
code
Lysosomal Staining Kit - Blue Fluorescence
Live adherent & suspension cells
360/445nm
ab112135
Lysosomal Staining Kit - Green Fluorescence
Live adherent & suspension cells
490/525nm
ab112136
Lysosomal Staining Kit - Green Fluorescence
(large stokes shift)
Lysosomal Staining Kit - Orange Fluorescence
Live adherent & suspension cells
405/480nm
ab112138
Live adherent & suspension cells
540/560nm
ab138895
Lysosomal Staining Kit - Red Fluorescence
Live adherent & suspension cells
540/590nm
ab112137
Lysosomal Staining Kit - Deep Red Fluorescence
Live adherent & suspension cells
590/620nm
ab138896
Product
name
Sample
type
Ex/Em
Mitochondrial Staining Kit - Green Fluorescence
Live adherent & suspension cells
490/520nm
ab112143
Mitochondrial Staining Kit - Orange Fluorescence
(large stokes shift)
Live adherent & suspension cells
405/550nm
ab138897
Mitochondrial staining kits
16
Color
Product
code
Resources: www.abcam.com/cytopainter
Mitochondrial staining kits (continued)
Product
name
Sample
type
Ex/Em
Color
Product
code
Mitochondrial Staining Kit - Orange Fluorescence
Live adherent & suspension cells
545/575nm
ab112144
Mitochondrial Staining Kit - Red Fluorescence
Live adherent & suspension cells
580/600nm
ab112145
Product
name
Sample
type
Ex/Em
Cell Tracking Staining Kit - Blue Fluorescence
Live adherent & suspension cells
359/461 nm
ab138890
Cell Tracking Staining Kit - Green Fluorescence
Live adherent & suspension cells
490/520nm
ab138891
Cell Tracking Staining Kit - Orange Fluorescence
Live adherent & suspension cells
540/560nm
ab138892
Cell Tracking Staining Kit - Red Fluorescence
Live adherent & suspension cells
575/600nm
ab138893
Cell Tracking Staining Kit - Deep Red Fluorescence
Live adherent & suspension cells
637/650nm
ab138894
Product
name
Sample
type
Ex/Em
CFSE - Cell Labeling Kit
Adherent cells, suspension cells
492/517nm
ab112143
CyTrack Orange - nuclear & cytoplasmic staining
Adherent cells, suspension cells
457-488-549/615nm
ab109203
DRAQ5 - nuclear staining
Adherent cells, suspension cells
646/697nm
ab108410
DRAQ7 - nuclear staining
Fixed adherent and suspension
cells, fixed tissue sections
Fixed adherent and suspension
cells, fixed tissue sections
Fixed adherent and suspension
cells, fixed tissue sections
644/697nm
ab109202
359/461 nm
ab104139
536 / 617 nm
ab14083
Cell tracking staining kits
Color
Product
code
Fluoroshield Mounting Medium with DAPI
Propidium Iodide
CytoPainter F-actin staining kit
(blue) (ab112124)
Color
Cellular staining
Other cellular staining reagents
Product
code
P
hi rod
gh u
lig ct
ht
Mouse embryonic stem cell-differentiated embryoid bodies (EBs) were
stained with a rabbit anti-laminin alpha antibody, detected with Goat polyclonal
Secondary Antibody to Rabbit IgG-H&L (FITC) (ab97050). Actin filaments were
stained with CytoPainter F-actin staining kit (Blue) (ab112124), and nuclei were
counterstained with DRAQ7™ (ab109202).
Image courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School
Resources: www.abcam.com/cytopainter
: MitoSciences product
17
EpiSeeker Epigenetic kits
Epigenetics is the study of heritable changes in
gene expression caused by mechanisms other than
variances in DNA sequence.
Abcam’s range of epigenetic kits, called
EpiSeeker, offer researchers simple tools for
analyzing epigenetic modifications in the most
reliable and time saving manner.
• Simple and reliable assays
• Available in colorimetric / fluorometric format
• Multiple sizes: 48 / 96 well plate size
• Ready to go: all essential components present in the kit
Sample preparation kits
EpiSeeker epigenetic kits
Our sample preparation kits have been optimized to be used with our EpiSeeker range of epigenetic kits and
ensure the best possible starting material for your experiments.
• Suitable for cultured cells, fresh or frozen tissue, blood and other fluids
• Preparation in less than 1 hour
Product
name
Application
Chromatin Extraction Kit
Chromatin or DNA-protein complex for ChIP, in vitro protein-DNA binding
assays and nuclear enzyme assays.
Histone protein for histone detection
Nuclear protein for enzyme assay activity or protein detection
Nuclear protein (no nucleic acid) for protein detection
Whole cell protein for enzyme assay activity or protein detection
Histone Extraction Kit
Nuclear Extraction Kit
Nuclear (nucleic acid-free) Extraction Kit
Whole Cell Extraction Kit
Product
code
ab117152
ab113476
ab113474
ab113477
ab113475
DNA methylation kits
DNA methylation in mammals occurs primarily on the fifth carbon residue of cytosine (5-methylcytosine or
5-methylcytidine, 5-mC) of CpG dinucleotides. This reversible covalent addition of a methyl group is important
for establishing chromatin structures, imprinting and gene regulation.
Bisulfite modification kits
Bisulfite modification, also known as bisulfite
conversion, is one of the most useful tools for
analyzing methylation of cytosine residues. This
method is based on treating DNA with sodium
bisulfite in order to determine its methylation
pattern: treatment of DNA with sodium bisulfite
(a process called sulphonation) leads to the
deamination of cytosine residues and converts
them to uracil, while 5-methylcytosine residues
remain the same.
Bisulfite-mediated conversion of cytosine to uracil
18
Resources: www.abcam.com/episeeker
Bisulfite-modified DNA is generally analyzed by
PCR, which can be performed under methylation(MS-PCR) or non-methylation-specific conditions.
The treatment will generate specific changes in
the DNA sequence that will depend on the
methylation status of individual cytosine residues,
providing single-nucleotide resolution information
about the DNA region: cytosine residues that
have been converted to uracil will be detected as
thymidine residues whereas methylated cytosine
residues will be detected as cytosines.
Bisulfite treatment for analysis of methylated DNA
EpiSeeker epigenetic kits
• Conversion from purified DNA or directly from cells
• Results in only 3 hours
• Treated DNA is suitable for MS-PCR, RT-PCR, pyrosequencing and methylation microarray
Bisulfite modification Kits
Product
name
Application
Methylated control DNA kit
DNA purification & modification kit
Bisulfite modification kit - whole cell
Fast DNA modification kit
Methylation-positive control for bisulfite conversion treatments
DNA isolation and bisulfite conversion from sample (two separate steps)
Direct bisulfite conversion from cells or tissue
Bisulfite conversion of purified DNA
Product
code
ab117125
ab117124
ab117126
ab117127
DNA methylation kits
DNA methylation can also be studied by using specific antibodies against 5-methylcytosine or its hydroxylated
form, 5-hydroxymethylcytosine (5-hmC). These antibodies can be used to either directly quantify 5-mC or
5-hmC in an ELISA-type assay or to enrich a methylated DNA section for specific immunoprecipitation.
• Directly quantify methylated or hydroxymethylated DNA in less than 4 hours
• Perform specific methylated or hydroxylated DNA immunoprecipitation using a variety of starting material:
DNA, cells or tissue
• Detect specific binding activity of MBD2 (methyl-CpG binding domain protein 2) to methylated DNA, or use
MBD2 to capture methylated DNA.
Product
name
Application
MBD2 Binding Activity Quantification Assay Kit
MBD2 ChIP Kit
MBD2 ChIP Kit - Tissue
Methylated DNA Immunoprecipitation (MeDIP)
ChIP Kit - DNA
Methylated DNA Immunoprecipitation (MeDIP)
ChIP Kit
Methylated DNA Immunoprecipitation (MeDIP)
ChIP Kit - tissue
Hydroxymethylated DNA Immunoprecipitation
(hMeDIP) ChIP Kit
Methylated DNA Quantification Kit (Colorimetric)
Methylated DNA Quantification Kit (Fluorometric)
Hydroxymethylated DNA Quantification Kit
(Colorimetric)
Hydroxymethylated DNA Quantification Kit
(Fluorometric)
Measurement of MBD2 binding activity to methylated DNA
Immunoprecipitation of MBD2 from cells
Immunoprecipitation of MBD2 from tissue
Immunoprecipitation of 5-mC (methylated cytosine) residue from nuclear extracts
ab113473
ab113450
ab113451
ab117133
Immunoprecipitation of 5-mC (methylated cytosine) residue from cells
ab117135
Immunoprecipitation of 5-mC (methylated cytosine) residue from tissue
ab117136
Immunoprecipitation of 5-hmC (hydroxymethylated cytosine) residue
ab117134
Colorimetric measurement of total methylated DNA
Fluorometric measurement of total methylated DNA
Colorimetric measurement of total hydroxymethylated DNA
ab117128
ab117129
ab117130
Fluorometric measurement of total hydroxymethylated DNA
ab117131
Resources: www.abcam.com/episeeker
Product
code
19
DNMT and demethylase activity kits
DNA methyltransferases (DNMT) and demethyltransferases (also known as demethylases) are the enzymes
responsible for regulating DNA methylation on CpG dinucleotides.
• Quantify total or specific DNMT activity in less than 4 hours
• Colorimetric or fluorometric format available
Product
name
Application
Product
code
DNMT Activity Quantification Kit (Colorimetric)
DNMT Activity Quantification Kit (Fluorometric)
DNMT1A Assay Kit
DNMT3A Assay Kit
DNMT3B Assay Kit
DNMT1 Inhibitor Screening Assay Kit
DNMT3B Inhibitor Screening Assay Kit
DNA demethylase (total) activity
Quantification Assay Kit
Colorimetric measurement of DNMT activity
Fluorometric measurement of DNMT activity
Determination of DNMT1 protein amount
Determination of DNMT3A protein amount
Determination of DNMT3B protein amount
Screening of DNMT1 inhibitor molecules
Screening of DNMT3B inhibitor molecules
Measurement of total activity of DNA demethylase enzymes
ab113467
ab113468
ab113469
ab113470
ab113471
ab113465
ab113466
ab113472
EpiSeeker epigenetic kits
Histone modification detection and quantification kits
Posttranslational histone modifications are important epigenetic modifiers: acetylation, methylation,
phosphorylation or sumoylation are reversible covalent processes that can occur simultaneously on a given
histone. They activate or repress gene transcription by modulating the accessibility of proteins to chromatin.
• More than 100 kits available to detect a range of histone modifications
• Quantify key residue modifications in less than 3 hours
• Colorimetric or fluorometric format available
Product
name
Application
Product
code
Histone H3 total Quantification Kit (Colorimetric)
Histone H3 total Quantification Kit (Fluorometric)
Colorimetric measurement of total Histone H3, independently of its modification state
Fluorometric measurement of total Histone H3, independently of its modification state
ab115091
ab115092
Histone acetylation detection kits
20
Product
name
Application
Product
code
Histone Acetyl Transferase Activity
Assay Kit (Colorimetric)
Histone Acetyl Transferase Inhibitor
Screening Assay Kit
Histone H3 Acetylation Assay Kit
Histone H4 Acetylation Assay Kit
Histone H3 Total Acetylation Detection
Fast Kit (Colorimetric)
Histone H3 Total Acetylation Detection
Fast Kit (Fluorometric)
Histone H4 Total Acetylation Detection
Fast Kit (Colorimetric)
Histone H4 Total Acetylation Detection
Fast Kit (Fluorometric)
Histone H3 (acetyl K9) Assay Kit (In situ)
Histone H3 (acetyl K9) Quantification
Assay Kit (Colorimetric)
Histone H3 (acetyl K9) Quantification
Assay Kit (Fluorometric)
Histone H3 (acetyl K14) Quantification
Assay Kit (Colorimetric)
Histone H3 (acetyl K14) Quantification
Assay Kit (Fluorometric)
Histone H3 (acetyl K18) Quantification
Assay Kit (Colorimetric)
Colorimetric detection of HAT activity
ab65352
Fluorometric evaluation of HAT inhibitors
ab133099
Measurement of global acetylation of Histone H3
Measurement of global acetylation of Histone H4
Detection and quantification of acetylated Histone H3
ab115102
ab115103
ab115124
Detection and quantification of acetylated Histone H4
ab131561
Detection and quantification of acetylated Histone H4
ab115125
Detection and quantification of acetylated Histone H4
ab131562
Measurement of acetylated K9 on Histone H3 from cells
Colorimetric measurement of acetylated K9 on Histone H3
ab115101
ab115104
Fluorometric measurement of acetylated K9 on Histone H3
ab115105
Colorimetric measurement of acetylated K14 on Histone H3
ab115106
Fluorometric measurement of acetylated K14 on Histone H3
ab115107
Colorimetric measurement of acetylated K18 on Histone H3
ab115108
Resources: www.abcam.com/episeeker
Histone acetylation detection kits (continued)
Application
Product
code
Histone H3 (acetyl K18) Quantification
Assay Kit (Fluorometric)
Histone H3 (acetyl K23) Quantification
Assay Kit (Colorimetric)
Histone H3 (acetyl K23) Quantification
Assay Kit (Fluorometric)
Histone H3 (acetyl K36) Quantification
Assay Kit (Colorimetric)
Histone H3 (acetyl K36) Quantification
Assay Kit (Fluorometric)
Histone H3 (acetyl K56) Quantification
Assay Kit (Colorimetric)
Histone H3 (acetyl K56) Quantification
Assay Kit (Fluorometric)
Histone H4 (acetyl K5) Quantification
Assay Kit (Colorimetric)
Histone H4 (acetyl K5) Quantification
Assay Kit (Fluorometric)
Histone H4 (acetyl K8) Quantification
Assay Kit (Colorimetric)
Histone H4 (acetyl K8) Quantification
Assay Kit (Fluorometric)
Histone H4 (acetyl K12) Quantification
Assay Kit (Colorimetric)
Histone H4 (acetyl K12) Quantification
Assay Kit (Fluorometric)
Histone H4 (acetyl K16) Quantification
Assay Kit (Colorimetric)
Histone H4 (acetyl K16) Quantification
Assay Kit (Fluorometric)
Fluorometric measurement of acetylated K18 on Histone H3
ab115109
Colorimetric measurement of acetylated K23 on Histone H3
ab115110
Fluorometric measurement of acetylated K23 on Histone H3
ab115111
Colorimetric measurement of acetylated K36 on Histone H3
ab115112
Fluorometric measurement of acetylated K36 on Histone H3
ab115113
Colorimetric measurement of acetylated K56 on Histone H3
ab115114
Fluorometric measurement of acetylated K56 on Histone H3
ab115115
Colorimetric measurement of acetylated K5 on Histone H4
ab115116
Fluorometric measurement of acetylated K5 on Histone H4
ab115117
Colorimetric measurement of acetylated K8 on Histone H4
ab115118
Fluorometric measurement of acetylated K8 on Histone H4
ab115119
Colorimetric measurement of acetylated K12 on Histone H4
ab115122
Fluorometric measurement of acetylated K12 on Histone H4
ab115123
Colorimetric measurement of acetylated K16 on Histone H4
ab115120
Fluorometric measurement of acetylated K16 on Histone H4
ab115121
EpiSeeker epigenetic kits
Product
name
Histone deacetylase detection kits
Product
name
Application
SIRT1 Screening Assay Kit (FRET-based)
SIRT2 Screening Assay Kit
SIRT3 Screening Assay Kit
SIRT6 Screening Assay Kit
FRET-based evaluation of
Fluorometric evaluation of
Fluorometric evaluation of
Fluorometric evaluation of
Product
code
SIRT1 inhibitor or activator compounds
SIRT2 inhibitor or activator compounds
SIRT3 inhibitor or activator compounds
SIRT6 inhibitor or activator compounds
ab133126
ab133082
ab133129
ab133083
Histone methyltransferase detection kits
Product
name
Application
Histone H3 (K4) Methyltransferase Activity
Quantification Assay Kit
Histone H3 (K9) Methyltransferase Activity
Quantification Assay Kit
Histone H3 (K27) Methyltransferase Activity
Quantification Assay Kit
Histone H3 (K4) Demethyltransferase Activity
Quantification Assay Kit
Histone H3 (K9) Demethyltransferase Activity
Quantification Assay Kit
Measurement of Histone H3 (K4) histone methyltransferase activity
ab113452
Measurement of Histone H3 (K9) histone methyltransferase activity
ab113453
Measurement of Histone H3 (K27) histone methyltransferase activity
ab113454
Measurement of Histone H3 (K4) histone demethylase activity
ab113455
Measurement of Histone H3 (K9) histone demethylase activity
ab113458
Resources: www.abcam.com/episeeker
Product
code
21
Histone demethylase activity detection kits
Product
name
Application
Product
code
KDM1/LDS1 Inhibitor Assay Kit
KDM1/LDS1 Activity Quantification Kit
KDM1/LDS1 Activity Quantification Kit (Colorimetric)
KDM1/LDS1 Activity Quantification Kit (Fluorometric)
KDM4/JMJD2 Activity Quantification Kit (Colorimetric)
KDM4/JMJD2 Activity Quantification Kit (Fluorometric)
KDM5/JARID Activity Quantification Kit (Colorimetric)
KDM5/JARID Activity Quantification Kit (Fluorometric)
Screening of KDM1/LSD1 inhibitor molecules
Measurement of KDM1/LSD1 activity (indirect detection)
Colorimetric measurement of KDM1/LSD1 activity
Fluorometric measurement of KDM1/LSD1 activity
Colorimetric measurement of KDM2/JMJD2 activity
Fluorometric measurement of KDM2/JMJD2 activity
Colorimetric measurement of KDM5/JARID activity
Fluorometric measurement of KDM5/JARID activity
ab113456
ab113457
ab113459
ab113460
ab113461
ab113462
ab113463
ab113464
EpiSeeker epigenetic kits
Histone methylation detection kits
22
Product
name
Application
Product
code
Histone H3 (methyl K4) Assay Kit
Histone H3 (methyl K4) Assay Kit (In Situ)
Histone H3 (methyl K9) Assay Kit
Histone H3 (methyl K9) Assay Kit (In Situ)
Histone H3 (methyl K27) Assay Kit
Histone H3 (tri methyl K27) Assay Kit
Histone H3 (tri methyl K27) Assay Kit (In Situ)
Histone H3 (mono-methyl K4) Quantification
Kit (Colorimetric)
Histone H3 (mono-methyl K4) Quantification
Kit (Fluorometric)
Histone H3 (di-methyl K4) Quantification
Kit (Colorimetric)
Histone H3 (di-methyl K4) Quantification
Kit (Fluorometric)
Histone H3 (tri-methyl K4) Quantification
Kit (Colorimetric)
Histone H3 (tri-methyl K4) Quantification
Kit (Fluorometric)
Histone H3 (pan-methyl K4) Quantification
Kit (Colorimetric)
Histone H3 (pan-methyl K4) Quantification
Kit (Fluorometric)
Histone H3 (mono-methyl K9) Quantification
Kit (Colorimetric)
Histone H3 (mono-methyl K9) Quantification
Kit (Fluorometric)
Histone H3 (di-methyl K9) Quantification Kit (Colorimetric)
Histone H3 (di-methyl K9) Quantification
Kit (Fluorometric)
Histone H3 (tri-methyl K9) Quantification Kit (Colorimetric)
Histone H3 (tri-methyl K9) Quantification
Kit (Fluorometric)
Histone H3 (pan-methyl K9) Quantification
Kit (Colorimetric)
Histone H3 (pan-methyl K9) Quantification
Kit (Fluorometric)
Histone H3 (mono-methyl K27) Quantification
Kit (Colorimetric)
Histone H3 (mono-methyl K27) Quantification
Kit (Fluorometric)
Histone H3 (di-methyl K27) Quantification Kit (Colorimetric)
Histone H3 (di-methyl K37) Quantification
Kit (Fluorometric)
Histone H3 (tri-methyl K27) Quantification Kit (Colorimetric)
Histone H3 (tri-methyl K27) Quantification
Kit (Fluorometric)
Histone H3 (pan-methyl K27) Quantification
Kit (Colorimetric)
Histone H3 (pan-methyl K27) Quantification
Kit (Fluorometric)
Measurement of methylated K4 on Histone H3
Measurement of methylated K4 on Histone H3 directly from cells
Measurement of methylated K9 on Histone H3
Measurement of methylated K9 on Histone H3 directly from cells
Measurement of methylated K27 on Histone H3
Measurement of tri-methylated K27 on Histone H3
Measurement of tri-methylated K27 on Histone H3 from cells
Colorimetric measurement of mono-methylated K4 on Histone H3
ab115048
ab115046
ab115049
ab115047
ab115050
ab115051
ab115045
ab115054
Fluorometric measurement of mono-methylated K4 on Histone H3
ab115055
Colorimetric measurement of di-methylated K4 on Histone H3
ab115052
Fluorometric measurement of di-methylated K4 on Histone H3
ab115053
Colorimetric measurement of tri-methylated K4 on Histone H3
ab115056
Fluorometric measurement of tri-methylated K4 on Histone H3
ab115057
Colorimetric measurement of pan-methylated K4 on Histone H3
ab115058
Fluorometric measurement of pan-methylated K4 on Histone H3
ab115059
Colorimetric measurement of mono-methylated K9 on Histone H3
ab115060
Fluorometric measurement of mono-methylated K9 on Histone H3
ab115061
Colorimetric measurement of di-methylated K9 on Histone H3
Fluorometric measurement of di-methylated K9 on Histone H3
ab115062
ab115063
Colorimetric measurement of tri-methylated K9 on Histone H3
Fluorometric measurement of tri-methylated K9 on Histone H3
ab115064
ab115065
Colorimetric measurement of pan-methylated K9 on Histone H3
ab115066
Fluorometric measurement of pan-methylated K9 on Histone H3
ab115067
Colorimetric measurement of mono-methylated K27 on Histone H3
ab115068
Fluorometric measurement of mono-methylated K27 on Histone H3
ab115069
Colorimetric measurement of di-methylated K27 on Histone H3
Fluorometric measurement of di-methylated K27 on Histone H3
ab115070
ab115071
Colorimetric measurement of tri-methylated K27 on Histone H3
Fluorometric measurement of tri-methylated K27 on Histone H3
ab115072
ab115073
Colorimetric measurement of pan-methylated K27 on Histone H3
ab115074
Fluorometric measurement of pan-methylated K27 on Histone H3
ab131560
Resources: www.abcam.com/episeeker
Histone methylation detection kits
Application
Product
code
Histone H3 (mono-methyl K36)
Quantification Kit (Colorimetric)
Histone H3 (mono-methyl K36)
Quantification Kit (Fluorometric)
Histone H3 (di-methyl K36)
Quantification Kit (Colorimetric)
Histone H3 (di-methyl K36)
Quantification Kit (Fluorometric)
Histone H3 (tri-methyl K36)
Quantification Kit (Colorimetric)
Histone H3 (tri-methyl K36)
Quantification Kit (Fluorometric)
Histone H3 (pan-methyl K36)
Quantification Kit (Colorimetric)
Histone H3 (pan-methyl K36)
Quantification Kit (Fluorometric)
Histone H3 (mono-methyl K79)
Quantification Kit (Colorimetric)
Histone H3 (mono-methyl K79)
Quantification Kit (Fluorometric)
Histone H3 (di-methyl K79)
Quantification Kit (Colorimetric)
Histone H3 (di-methyl K79)
Quantification Kit (Fluorometric)
Histone H3 (tri-methyl K79)
Quantification Kit (Colorimetric)
Histone H3 (tri-methyl K79)
Quantification Kit (Fluorometric)
Histone H3 (pan-methyl K79)
Quantification Kit (Colorimetric)
Histone H3 (pan-methyl K79)
Quantification Kit (Fluorometric)
Histone H4 (mono-methyl K20)
Quantification Kit (Colorimetric)
Histone H4 (mono-methyl K20)
Quantification Kit (Fluorometric)
Histone H4 (di-methyl K20)
Quantification Kit (Colorimetric)
Histone H4 (di-methyl K20)
Quantification Kit (Fluorometric)
Histone H4 (tri-methyl K20)
Quantification Kit (Colorimetric)
Histone H4 (tri-methyl K20)
Quantification Kit (Fluorometric)
Histone H4 (pan-methyl K20)
Quantification Kit (Colorimetric)
Histone H4 (pan-methyl K20)
Quantification Kit (Fluorometric)
Colorimetric measurement of mono-methylated K36 on Histone H3
ab115075
Fluorometric measurement of mono-methylated K36 on Histone H3
ab115076
Colorimetric measurement of di-methylated K36 on Histone H3
ab115077
Fluorometric measurement of di-methylated K36 on Histone H3
ab115078
Colorimetric measurement of tri-methylated K36 on Histone H3
ab115079
Fluorometric measurement of tri-methylated K36 on Histone H3
ab115080
Colorimetric measurement of pan-methylated K36 on Histone H3
ab115081
Fluorometric measurement of pan-methylated K36 on Histone H3
ab115082
Colorimetric measurement of mono-methylated K79 on Histone H3
ab115083
Fluorometric measurement of mono-methylated K79 on Histone H3
ab115084
Colorimetric measurement of di-methylated K79 on Histone H3
ab115085
Fluorometric measurement of di-methylated K79 on Histone H3
ab115086
Colorimetric measurement of tri-methylated K79 on Histone H3
ab115087
Fluorometric measurement of tri-methylated K79 on Histone H3
ab115088
Colorimetric measurement of pan-methylated K79 on Histone H3
ab115089
Fluorometric measurement of pan-methylated K79 on Histone H3
ab115090
Colorimetric measurement of mono-methylated K20 on Histone H4
ab115093
Fluorometric measurement of mono-methylated K20 on Histone H4
ab115094
Colorimetric measurement of di-methylated K20 on Histone H4
ab115095
Fluorometric measurement of di-methylated K20 on Histone H4
ab115096
Colorimetric measurement of tri-methylated K20 on Histone H4
ab115097
Fluorometric measurement of tri-methylated K20 on Histone H4
ab115098
Colorimetric measurement of pan-methylated K20 on Histone H4
ab115099
Fluorometric measurement of pan-methylated K20 on Histone H4
ab115100
EpiSeeker epigenetic kits
Product
name
Histone phosphosphorylation detection kits
Product
name
Application
Histone H3 (phospho S10)
Assay Kit (In Situ)
Histone H3 (phospho S10)
Assay Kit (Colorimetric)
Histone H3 (phospho S10)
Assay Kit (Fluorometric)
Histone H3 (phospho S28)
Assay Kit (Colorimetric)
Histone H3 (phospho S28)
Assay Kit (Fluorometric)
Measurement of phosphorylated S10 on Histone H3 directly from cells
ab115126
Colorimetric measurement of phosphorylated S10 on Histone H3
ab115127
Fluorometric measurement of phosphorylated S10 on Histone H3
ab115128
Colorimetric measurement of phosphorylated S28 on Histone H3
ab115129
Fluorometric measurement of phosphorylated S28 on Histone H3
ab115130
Resources: www.abcam.com/episeeker
Product
code
23
Sumoylation detection kits
Product
name
Application
Product
code
Global Protein Sumoylation Assay Kit
HDAC1 Sumoylation Assay Kit
Measurement of global sumoylated protein
Measurement of global sumoylated HDAC1
ab115131
ab115132
DNA/ Protein interaction
DNA-binding proteins are critical for cellular functions such as gene transcription and DNA replication, as well
as epigenetic silencing.
Identifying the genetic targets of these proteins and how they interact with their corresponding DNA sequences
is essential for understanding cellular functions. In mammalian cells, interactions between DNA-binding proteins
such as transcription factors and promoter sequences are responsible for establishing spatial and temporal
gene expression patterns.
EpiSeeker epigenetic kits
In vitro DNA/Protein interaction detection
• Quantification of in vitro DNA-protein interaction
• Choose between specific transcription factors or design your own dsDNA oligo for your sequence of interest
in vitro DNA/Protein interaction
Product
name
Application
DNA/ protein Binding Assay Kit (Colorimetric)
DNA/ protein Binding Assay Kit (Fluorometric)
ATF2 (pT69/pT71) Transcription Factor Assay Kit
CREB (pS133) Transcription Factor Assay Kit
ChREBP Transcription Factor Assay Kit
Colorimetric detection of interaction of transcription factors with dsDNA of your own design
Fluorometric detection of interaction of transcription factors with dsDNA of your own design
Colorimetric detection of specific ATF2 binding activity to dsDNA cAMP response elements
Colorimetric detection of specific CREB binding activity to dsDNA cAMP response elements
Colorimetric detection of specific ChREBP binding activity to dsDNA carbohydrate
response elements
Colorimetric detection of specific HIF-1 alpha binding activity to dsDNA HIF-1 alpha
response elements
Colorimetric detection of specific NF-kB p50 subunit binding activity to dsDNA NF-kB
response elements
Colorimetric detection of NF-kB binding activity to dsDNA NF-kB response elements
Colorimetric detection of specific NF-kB p65 subunit binding activity to dsDNA NF-kB
response elements
Colorimetric detection of-- PPARs binding activity to dsDNA PPAR response elements
HIF-1 alpha Transcription Factor Assay Kit
NF-kB p50 Transcription Factor Assay Kit
NF-kB p50/p65 Transcription Factor Assay Kit
NF-kB p65 Transcription Factor Assay Kit
PPAR (alpha, delta, gamma) Transcription Factor
Assay Kit
PPAR alpha Transcription Factor Assay Kit
PPAR delta Transcriprtion Factor Assay Kit
PPAR gamma Transcription Factor Assay Kit
SREBP-1 Transcription Factor Assay Kit
SREBP-2 Transcription Factor Assay Kit
24
Colorimetric detection of
response elements
Colorimetric detection of
response elements
Colorimetric detection of
response elements
Colorimetric detection of
response elements
Colorimetric detection of
response element
Product
code
ab117139
ab117140
ab133074
ab133117
ab133103
ab133104
ab133105
ab133128
ab133112
ab133113
specific PPAR alpha binding activity to dsDNA PPAR
ab133107
specific PPAR delta binding activity to dsDNA PPAR
ab133106
specific PPAR gamma binding activity to dsDNA PPAR
ab133101
specific SREBP-1 binding activity to dsDNA SREBP
ab133125
specific SREBP-2 binding activity to dsDNA SREBP
ab133111
Resources: www.abcam.com/episeeker
In vivo DNA/Protein interaction detection
ChIP (Chromatin Immunoprecipitation) is an established and versatile tool for characterizing in vivo how a
particular protein binds to specific sequences of genomic loci such as gene promoters.
• Choose between pre-tested ChIP-grade antibodies or use your antibody of choice
• Enriched DNA ready for downstream processing in only 5 hours
• All components included (except cross-linking agents )
in vivo DNA/Protein interaction
Application
Product
code
ChIP Kit - One Step
ChIP Kit - Plants
ChIP Kit - Histone H3 (acetyl)
ChIP Kit - Histone H3 (acetyl) - Tissue
ChIP Kit - Histone H4 (acetyl)
ChIP Kit - Histone H4 (acetyl) - Tissue
ChIP Kit - Histone H3 (methyl K4)
ChIP Kit - Histone H3 (methyl K4) - Tissue
ChIP Kit - Histone H3 (methyl K9)
ChIP Kit - Histone H3 (methyl K9) - Tissue
ChIP Kit - Histone H3 (tri-methyl K9)
ChIP Kit - Histone H3 (methyl K27)
ChIP Kit - Histone H3 (methyl K27) - Tissue
MBD2 ChIP Kit
MBD2 ChIP Kit - Tissue
Methylated DNA Immunoprecipitation (MeDIP)
ChIP Kit - DNA
Methylated DNA Immunoprecipitation (MeDIP)
ChIP Kit
Methylated DNA Immunoprecipitation (MeDIP)
ChIP Kit - tissue
Hydroxymethylated DNA Immunoprecipitation
(hMeDIP) ChIP Kit
One step immunoprecipitation of mammalian DNA
Immunoprecipitation of plant DNA
Immunoprecipitation of pan-acetylated Histone H3
Immunoprecipitation of pan-acetylated Histone H3 from tissue
Immunoprecipitation of pan-acetylated Histone H4
Immunoprecipitation of pan-acetylated Histone H4 from tissue
Immunoprecipitation of pan-methylated K4 on Histone H3
Immunoprecipitation of pan-methylated K4 on Histone H3 from tissue
Immunoprecipitation of pan-methylated K9 on Histone H3
Immunoprecipitation of pan-methylated K9 on Histone H3 from tissue
Immunoprecipitation of tri-methylated K9 on Histone H3
Immunoprecipitation of pan-methylated K27 on Histone H3
Immunoprecipitation of pan-methylated K27 on Histone H3 from tissue
Immunoprecipitation of MBD2 from cells
Immunoprecipitation of MBD2 from tissue
Immunoprecipitation of 5-mC (methylated cytosine) residue from nuclear extracts
ab117138
ab117137
ab117148
ab117150
ab117149
ab117151
ab117143
ab117145
ab117141
ab117144
ab117142
ab117146
ab117147
ab113450
ab113451
ab117133
Immunoprecipitation of 5-mC (methylated cytosine) residue from cells
ab117135
Immunoprecipitation of 5-mC (methylated cytosine) residue from tissue
ab117136
Immunoprecipitation of 5-hmC (hydroxymethylated cytosine) residue
ab117134
EpiSeeker epigenetic kits
Product
name
Did you miss our
webinars on ChIP?
Watch them in our blog
www.abcam.com/blog
Resources: www.abcam.com/episeeker
25
Metabolism
Metabolic processes can be divided into two
main categories: anabolic processes, which
consume energy for the synthesis of cellular
components such as proteins and nucleic acids
and catabolic processes that release energy
by breaking down organic compounds.
Abcam offers assay kits to quickly and
reproducibly measure metabolic processes in
96-well microplates by fluorometric/colorimetric
detection methods.
Cancer metabolism
Glucose
Antibody
Glucose
Glutamine
GLUT1
MYC
HIF
p53
NUCLEOTIDE
SYNTHESIS
Glucose-6P
Ribose-5P
HIF
Carnitine Palmitoyltransferase 1
ATP Citrate Lyase
PI3K
Pyruvate
Dehydrogenase
Lactate
Dehydrogenase A
Fructose-1,6-P2
Acetyl-CoA
2 NADPH
Aldolase
Hu
P
Hu, Ms, Dm
Hu, Ms, Rt
ab32
ab22759
M
P
P
P
Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB
ELISA, IHC-P, WB
ICC/IF, WB
Conjugation, ELISA, ICC/IF, IHC-FoFr, WB
Ms
Rb
Rb
Goat
Hu, Ms, Rt
Hu
Hu
Hu, Rt, Gpig,
Ptto, Rb
ab40084
ab37593
ab103063
Mitochondrial Pyruvate
dehydrogenase kinase 1
p53 [PAb 240]
P
M
Hu, Rt
P
IHC-P, WB
ELISA, Flow Cyt, ICC, ICC/IF,
IHC (Methanol fixed), IHC-Fr, IHC-P, IP, WB
ELISA, IHC-P, WB
Rb
PFKL
Pyruvate Dehydrogenase E2
[mAbcam 69304]
Pyruvate Kinase
Ms
Rb
Hu, Ms, Rt, Dog
ab26
Hu, Ms
ab37583
M
P
WB
Conjugation, ELISA, IF, IP, WB
Ms
Goat
Hu
Hu, Rt, Rb
Hu, Ms, Rt
Kits
Size
Description
ENO1 Human Activity Assay Kit
1x96 tests
Assay to determine the activity of ENO1, a
glycolytic enzyme which is a homodimer
composed of 2 alpha subunits
Biochemicals
Size
Description
Diclofenac Sodium
FCCP
Lovastatin
NS 398
1g
10mg,50mg
10mg,50mg
10mg,50mg
Potent cyclo-oxygenase inhibitor
Mitochondrial oxidative phosphorylation inhibitor
Potent HMG CoA reductase inhibitor
COX-2 inhibitor
Glutamate
Pyruvate
Lactate
Ms
Ms
Rb
ab110321
ab51025
ab2101
ab73335
ab69304
ab6191
And for superb IF and ICC results use our pre-adsorbed secondary antibodies
Available conjugated to:
Cy3® and Cy5®
DyLight® 488, DyLight® 550, DyLight® 594, DyLight® 650
AMCA, FITC, PE, Texas Red® and TRITC
Pyruvate Dehydrogenase
Kinase 1
Pyruvate Kinase
AMPK
Product
code
Ms
Glutaminase
Fatty Acids
AMPK
Fructose-6P
P
Phosphofructokinase
Species
reactivity
Flow Cyt, ICC/IF, In-Cell ELISA, IP, WB
ELISA, ICC/IF, IP, WB
ChIP, ELISA, Flow Cyt, ICC, ICC/IF,
IHC (Methanol fixed), IHC-Fr, IHC-P,
IP, Purification, WB
ICC/IF, IHC (PFA fixed), IHC-Fr, IP, WB
PI3K
Fatty Acid Synthase
Glycerol-3P
p53
LIPID
SYNTHESIS
HIF-1
LIPID
SYNTHESIS
Glucose-6P
2 NADP+
2 NADPH
Phosphoglucose
Isomerase
Metabolism
MYC
Hexokinase 2
p53
Host
M
M
M
Fatty Acid Synthase
Glucose Transporter GLUT1
[SPM498]
Hexokinase II
HIF1 alpha
Lactate Dehydrogenase
PI3K
Glucose Transporter 1
Hexokinase
Clonality Applications
Aconitase 2 [6F12BD9]
AMPK alpha 1 +
AMPK alpha 2 [84.1]
c-Myc [9E10] - ChIP Grade
MYC
Product code
ab117994
Product code
ab120621
ab120081
ab120614
ab120295
Featured products
HIF
NADP+ Malate
TCA Cycle
Dihydroxyacetone-P
Glyceraldehyde-3phosphate Dehydrogenase
NUCLEOTIDE
SYNTHESIS
2 NADPH
ATP citrate lyase [EP704Y] (ab40793)
ETC
ROS
Clonality Applications
M
Flow Cyt, ICC/IF,
IHC-P, IP, WB
NADP+
Citrate
α-KG
1,3-Biphosphoglycerate
Phosphoglycerate
Kinase
Clonality Applications
P
IHC/IF, IHC-P, WB
MYC
3-Phosphoglycerate
Lactate
Lactate
Dehydrogenase
Pyruvate Dehydrogenase
Kinase 1
Pyruvate
HIF
HIF
Phosphoglucomutase
HIF
Malic
Enzyme 1
Acetyl-CoA
HIF
Enolase
Malate
Malate
e
Phosphoenolpyruvate
Pyruvate Kinase
HIF
Fumarate
Pyruvate
Succinate
α-Ketoglutarate
Acetyl-CoA
A
Malate
Dehydrogenase
Citrate
Synthase
Oxaloacetate
Mutant Fumarate
Hydratase
Electron
Transport
Chain
Carnitine Palmitoyltransferase 1
Fatty Acid Synthase
Pyruvate
Mutant Succinate
Dehydrogenase
MYC
Succinyl-CoA
Ligase
Clonality Applications
P
ICC/IF, IHC-P, WB
Citrate
Species cross reactivity
Hu
Host
Rb
Species cross reactivity
Hu, Ms, Rt
Host
Rb
Species cross reactivity
WB
p53 (ab2433)
Clonality Applications
P
Hu
2HG
Aconitase 2
HIF
Host
Rb
Glucose Transporter GLUT1 (ab32551)
Mutant Isocitrate
Dehydrogenase 1
Pyruvate
Dehydrogenase
MYC
Species cross reactivity
Hu, Ms, Rat
FATTY ACID
SYNTHESIS
Citrate
LIPID
SYNTHESIS
p53
2-Phosphoglycerate
Host
Rb
c-Myc antibody (ab86356)
HIF
Isocitrate
Pyrovate Dehydrogenase E2 (ab66511)
Mutant Isocitrate
Dehydrogenase 2
Clonality Applications
P
ELISA, IHC-P, WB
Host
Rb
Species cross reactivity
Hu, Ms, Rt, Dog
α-Ketoglutarate
α-Ketoglutarate
Dehydr
y oge
g na
Dehydrogenase
Succinyl-CoA
ROS
Pyruvate Kinase (ab118499)
Glutamate
Glutaminase
MYC
Clonality Applications
P
Conjugation, Dot Blot,
Flow Cyt, IHC-P, IP, WB
Host
Species cross reactivity
Goat
Rb
Glutamine
045_12_AH
Triosephosphate
Isomerase
Glyceraldehyde-3P
Discover more at abcam.com/cancer
Copyright © 2012 Abcam, All Rights Reserved. The Abcam logo is a registered trademark. All information / detail is correct at time of going to print.
Produced in collaboration with Meghan Verschoor (McMaster University, Hamilton, Ontario, Canada).
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Download our cancer
metabolism pathway card at
www.abcam.com/cancer-metabolism
• Comprehensive range of products for metabolite detection: allows measurement of metabolites involved in
sugar, lipid and amino acid metabolism
• Suitable to use in a broad range of samples to fit your experimental settings: live cells, cellular lysates, urine,
plasma or serum
• Easy to perform tests: just add, mix and read
• Suitable for high-throughput assays
Alcohol assay kits
26
Product
name
Sample
type
Alcohol Dehydrogenase Detection Kit
Aldehyde Quantification Assay Kit (Colorimetric)
Aldehyde Quantification Assay Kit (Fluorometric)
Ethanol Assay Kit
Free Glycerol Assay Kit
TC supernatants, urine, plasma, serum
Adherent cells, suspension cells, cell culture extracts
Cell culture extracts
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
Detection
Product
code
A/F
A
F
A/F
A/F
ab102533
ab112113
ab138882
ab65343
ab65337
Resources: www.abcam.com/metabolismkits
Amino acid assay kits
Sample
type
Alanine Transaminase Activity Assay Kit
Ammonia Assay Kit
Ammonia Assay Kit - Modified Berthelot - (Colorimetric)
Ascorbic Acid Assay Kit
Ascorbic Acid Assay Kit (Biological Samples)
Aspartate Assay Kit
Branched Chain Amino Acid
Acetyl CoA Assay Kit
Creatine Assay Kit
Creatinine Assay Kit
Glutamate Assay Kit
Glutamate Assay Kit (Fluorometric)
Glutamate Dehydrogenase Detection Kit
Glutamate Oxidase Assay Kit (Fluorometric)
Glutamic Oxaloacetic Transaminase Activity Assay Kit
Inosine Quantification Assay Kit (Flurometric)
L-Alanine Assay Kit
L-Amino Acid Asay Kit
L-Carnitine Assay Kit
Phenylalanine Assay Kit
Sarcosine Assay Kit
Trypsin Activity
Urea Assay Kit
Uric Assay Kit
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
Cell lysate, culture medium, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
Cell lysate, other biological fluids
TC supernatants, urine, plasma, serum
Cell lysate
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
Cell lysates, urine, plasma, serum
TC supernatants, urine, plasma, serum
Detection
Product
code
A/F
A
A
A/F
A
A/F
A
A/F
A/F
A/F
A
F
A
F
A
F
A/F
A/F
A/F
F
A/F
A
A
A/F
ab105134
ab83360
ab102509
ab65346
ab65656
ab102512
ab83374
ab102504
ab65339
ab65340
ab83389
ab138883
ab102527
ab138885
ab105135
ab126286
ab83394
ab65347
ab83392
ab83376
ab65338
ab102531
ab83362
ab65344
Detection
Product
code
A
A
FM
A
ab108830
ab108833
ab133119
ab108894
A
Metabolism
Product
name
Angiogenesis and coagulation assay kits
Product
name
Sample
type
Factor VII Human Chromogenic Activity Assay Kit
Factor X Human Chromogenic Activity Assay Kit
In Vitro Angiogenesis Assay Kit
Plasminogen activator Inhibitor Type 1 Human
Chromogenic Activity Kit
Renin Assay Kit (Fluorometric)
Renin Inhibitor Screening Assay Kit
Tissue type Plasminogen Activator Human
Chromogenic Activity Assay Kit
Tissue Factor Human Chromogenic Activity Assay Kit
Urokinase type Plasminogen Activator Human
Chromogenic Activity Assay Kit
Urokinase type Plasminogen Activator Human
Chromogenic Activity Assay Kit-Direct Assay
TC supernatants, plasma
TC supernatants, plasma
Adherent cells, suspension cells
TC supernatants
Cell lysate
Pharmarcological compounds
TC supernatants
A
ab138875
ab133092
ab108905
TC supernatants, plasma, tissue
TC supernatants, plasma
A
A
ab108906
ab108915
TC supernatants, plasma
A
ab65337
Detection
Product
code
A/F
L/B
A/F
L
A
ab83359
ab65313
ab83355
ab113849
ab109714
A
ab109716
A/F
F
A
A
A
ab102504
ab138889
ab83366
ab102506
ab65348
Coenzyme and cofactor assay kits
Product
name
Sample
type
ADP Assay Kit (Colorimetric/Fluorometric)
ADP/ATP Ratio Assay Kit (Bioluminescent)
ATP Assay Kit (Colorimetric/Fluorometric)
ATP Detection Assay Kit (Luminescent)
ATP synthase Enzyme Activity Microplate Assay Kit
Cell lysate, culture medium, urine, plasma, serum
Cell lysate, culture medium, urine, plasma, serum
Cell lysate, culture medium, urine, plasma, serum
Adherent cells, suspension cells
Cell culture extracts, tissue, suspension cells,
tissue extracts, cell lysate
Cell culture extracts, tissue, suspension cells,
tissue extracts, cell lysate
Cell lysate, urine, plasma, serum
Cell culture extracts, tissue extracts, cell lysate
Cell lysate, urine, plasma, serum
Cell lysate, culture medium, urine, plasma, serum
Cell lysate, urine, plasma, serum
ATP synthase Specific Activity Microplate Assay Kit
Coenzyme A Detection Kit
Coenzyme A Detection Kit (Fluorometric - Green)
Iron Assay Kit
Magnesium Detection Kit
NAD/NADH Assay Kit
Resources: www.abcam.com/metabolismkits
: MitoSciences product
27
Coenzyme and cofactor assay kits (continued)
Product
name
Sample
type
NADP/NADPH Assay Kit
PicoProbe™ Acetyl CoA Assay Kit
Zinc Quantification Kit
Cell lysate, urine, plasma, serum
Cell lysate, urine, plasma, serum
Cell lysate, culture medium, urine, plasma, serum
Detection
Product
code
A
F
A
ab65349
ab87546
ab102507
P
hi rod
gh u
lig ct
ht
ATP Assay Kit Colorimetric/Fluorometric (ab83355)
ATP is the key source of energy in the body. If ATP levels are too low to perform
basic metabolic processes, cells will die. The Abcam ATP Assay Kit
Colorimetric/Fluorometric is highly sensitive and can detect ATP levels as low as 50
picomoles. Moreover, it can be used in either a fluorescent or colorimetric microplate
reader for your convenience.
Example of standard calibration curve for ATP quantification following product protocol and using ATP standard
provided in Abcam’s ATP Assay Kit Colorimetric/Fluorometric (ab83355).
Metabolism
Ion and Metal assay kits
Product
name
Sample
type
Detection
Aconitase Assay Kit
Aconitase Enzyme Activity Microplate Assay Kit
Calcium Detection Kit (Colorimetric)
Calcium Assay Kit (Luminometric)
Calcium Quantification Kit (Fluorometric)
Fluo-8 Medium Removal Calcium Assay Kit
Fluo-8 No Wash Calcium Assay Kit
Rhod-4 Medium Removal No Wash Calcium Assay Kit
Rhod-4 No Wash Calcium Assay Kit
Chloride Assay Kit
Cobalt Quantification Kit
Hemin Assay Kit
Iron Assay Kit
Magnesium Detection Kit
Zinc Quantification Kit
TC supernatants, urine, plasma, serum
Cell culture extracts, Tissue
Cell lysate, culture medium, urine, plasma, serum
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
Adherent cells, suspension cells
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
Cell lysate, urine, plasma, serum
Cell lysate, culture medium, urine, plasma, serum
Cell lysate, culture medium, urine, plasma, serum
Product
code
C
A
A
L
F
F
F
F
F
A
A
A
A
A
A
ab83459
ab109712
ab102505
ab112114
ab112115
ab112128
ab112129
ab112156
ab112157
ab83372
ab102510
ab65332
ab83366
ab102506
ab102507
Detection
Product
code
Citric acid cycle/Intermediary metabolism assay kits
28
Product
name
Sample
type
Alpha Ketoglutarate (alpha KG) Assay Kit
Citrate Assay Kit
Free Glycerol Assay Kit
Fumarate Detection Kit
Glycerol Assay Kit (Cell-based)
Isocitrate Assay Kit
Isocitrate Dehydrogenase Assay Kit (Colorimetric)
Malate Assay Kit
NAD/NADH Assay Kit
NADP/NADPH Assay Kit
Oxaloacetate Assay Kit
PicoProbe™ Acetyl CoA Assay Kit
Pyruvate Assay Kit
Pyruvate Kinase (PK) Assay Kit
Pyruvate dehydrogenase (PDH) Combo
(Activity + Quantity) Microplate Assay Kit
Pyruvate dehydrogenase (PDH) Enzyme
Activity Microplate Assay Kit
Pyruvate dehydrogenase (PDH) Protein
Quantity Microplate Assay Kit
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
Adherent cells, cell culture media, TC supernatants
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
Cell lysate, urine, plasma, serum
Cell lysate, urine, plasma, serum
TC supernatants, urine, plasma, serum
Cell lysate, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
Cell culture extracts, tissue
A/F
A/F
A/F
A
A
A
A
A
A
A
A
F
A/F
A/F
A/C
ab83431
ab83396
ab65337
ab102516
ab133130
ab83424
ab102528
ab83391
ab65348
ab65349
ab83428
ab87546
ab65342
ab83432
ab110671
Cell culture extracts, tissue
A/C
ab109902
Cell culture extracts, tissue
A/C
ab110174
Resources: www.abcam.com/metabolismkits
Lipid metabolism/Diabetes assay kits
Sample
type
Acetylcholinesterase Assay Kit (Colorometric)
Acetylcholinesterase Assay Kit (Fluorometric - Green)
Acetylcholinesterase Assay Kit (Fluorometric - Red)
Acetyl CoA Assay Kit
Adipogenesis Assay Kit (Cell-based)
Adipogenesis Detection Kit
Adipolysis Assay Kit
beta Hydroxybutyrate (beta HB) Assay Kit
CETP Activity Assay Kit (Fluorometric)
CETP Inhibitor Drug Screening Kit
Cholesterol Assay Kit (Cell-based)
Cholesterol/Cholesteryl Ester Detection Kit
Cholesterol/Cholesteryl Ester Quantitation Kit
Choline/Acetylcholine Assay Kit
Free Fatty Acid Quantification Kit
Free Glycerol Assay Kit
Glucose Assay Kit
Glucose Detection Kit
Glucose Dehydrogenase Assay Kit (Colorimetric)
Glucose 6 Phosphate Assay Kit
Glucose 6 Phosphate Assay Kit (High Sensitvity)
Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric)
Glycerol Assay Kit (Cell-based)
Hepatic Lipid Accumulation/ Steatosis Assay Kit
HDL and LDL/VLDL Cholesterol Assay Kit
HDL Precipitation Buffer for ab65390
Lactate Dehydrogenase Assay Kit (Colorimetric)
LDL Uptake Assay Kit (Cell-based)
Lipase Detection kit I
Lipase Detection kit II - HTP
Lipase Detection Kit III (Fluorometric)
Lipid Hydroxyperoxide (LPO) Assay Kit
Lipid Peroxidation (MDA) Assay Kit
Lipoxygenase Inhibitor Screening Assay Kit
Lysyl Oxidase Activity Assay Kit (Fluorometric)
Myeloperoxidase (MPO) Inhibitor Screening Assay Kit
Phosphatidylcholine Assay Kit
PLTP Activity Assay Kit (Fluorometric)
PLTP Inhibitor Drug Screening Assay Kit
Triglyceride Quantification Kit
Cell culture extracts, whole blood
Cell culture extracts, whole blood
Cell culture extracts, whole blood
Cell lysate, urine, plasma, serum
Adherent cells
TC supernatants, urine, plasma, serum
Adherent cells, TC supernatants
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
Adherent cells
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
Adherent cells, cell culture media, TC supernatant
Adherent cells
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
Adherent cells
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
Cell culture extracts, plasma, tissue extracts
Cell culture extracts, plasma, tissue
Pharmarcological compounds
Adherent cells, suspension cells, cell culture extracts
Pharmarcological compounds
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
Lipid Peroxidation (MDA) Assay Kit (ab118970)
Lipid peroxidation is a well-established mechanism of cellular stress in higher
organisms, generally associated with diseases such as cancer, diabetes or
arteriosclerosis. The lipids in cell membranes are oxidized by free radicals (such as
hydroxyl radicals, ROS or nitric oxide), leading to the formation of lipid peroxides,
which are very unstable and will decompose to form complex, reactive by-products.
One of these by-products is malondialdehyde (MDA), generated by decomposition
of polyunsaturated fatty acid. MDA reacts with Thiobarbituric Acid (TBA) to generate
a MDA-TBA adduct that can be easily measured colorimetrically or fluorometrically
using ab118970.
Detection
Product
code
A
F
F
F
H
A/F
A/H
A
F
F
FM
A
A/F
A/F
A/F
A/F
A/F
A
A
A
F
A
A
A/H
A/F
A
IF
A
A
F
A
A/F
F
F
F
A/F
F
F
A/F
ab138871
ab138872
ab138873
ab87546
ab133102
ab102513
ab133115
ab83390
ab65383
ab65385
ab133116
ab102515
ab65359
ab65345
ab65341
ab65337
ab65333
ab102517
ab102532
ab83426
ab107923
ab102529
ab133130
ab133131
ab65390
ab105138
ab102526
ab133127
ab102524
ab102525
ab118969
ab133085
ab118970
ab133087
ab112139
ab133080
ab83377
ab65386
ab65387
ab65336
Metabolism
Product
name
P
hi rod
gh u
lig ct
ht
Example of standard calibration curve for malondialdehyde (MDA) following product protocol and using MDA
standard provided in Abcam’s Lipid Peroxidation (MDA) Assay Kit (ab118970).
Resources: www.abcam.com/metabolismkits
: MitoSciences product
29
Sugar metabolism assay kits
Product
name
Sample
type
Uric Acid Assay Kit
Amylase Assay Kit
ChREBP Translocation Assay Kit (Cell-based)
Dipeptidyl peptidase IV (DPP4) Inhibitor Screening Assay Kit
Fructose Assay Kit
Galactose Assay Kit
Galactose and Lactose Assay Kit
Glucose Assay Kit
Glucose Detection Kit
Glucose 6 Phosphate Assay Kit
Glucose 6 Phosphate Assay Kit (High Sensitvity)
Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric)
Glucose Dehydrogenase Detection Kit (Colorimetric)
Glucose Oxidase Assay Kit (Fluorometric)
Glucose Uptake Assay Kit (Colorimetric)
Glucose Uptake Assay Kit (Fluorometric)
Glucose and Sucrose Assay Kit
Hexokinase Assay Kit (Fluorometric)
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
Adherent cells
Pharmarcological compounds
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
Plasma, urine, other biological fluids
Adherent cells, suspension cells
Adherent cells, suspension cells
TC supernatants, urine, plasma, serum
Adherent cells, suspension cells, tissue,
tissue extracts, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
Adherent cells, suspension cells, cell culture extracts,
cell culture medium, TC supernatants
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
TC supernatants, urine, plasma, serum
Metabolism
D-Lactate Assay Kit (Colorimetric)
L-Lactate Assay Kit (Colorimetric)
L-Lactate Assay Kit (Fluorometric)
Lactate Dehydrogenase Assay Kit (Colorimetric)
Lactose Assay Kit
Malate Assay Kit
Maltose Assay Kit
Maltose and Glucose Assay Kit
Neuraminidase Assay Kit (Fluorometric - Blue)
Pyruvate Assay Kit
Pyruvate Kinase (PK) Assay Kit
Sialic Acid (NANA) Assay Kit
Starch Assay Kit
Detection
Product
code
A/F
A
H
F
A/F
A/F
A/F
A/F
A
A
F
A
A
F
A
F
A/F
F
ab65344
ab102523
ab133120
ab133081
ab83380
ab83382
ab83378
ab65333
ab102517
ab83426
ab107923
ab102529
ab102532
ab138884
ab136955
ab136956
ab65334
ab136957
A
A
A/F
A/F
A
A
A
A
F
ab83429
ab65331
ab65330
ab102526
ab83384
ab83391
ab83388
ab65335
ab138888
A
A/F
A/F
A/F
ab65342
ab83432
ab83375
ab83393
Detection
Product
code
Other metabolism assay kits
Product
name
Sample
type
Sucrose Assay Kit
Asparaginase Activity Assay Kit
Beta Secretase Activity Assay Kit (Fluorometric)
Beta Secretase Inhibitor Screening Assay Kit
Formaldehyde Assay Kit
Formate Assay Kit
MDR Assay Kit (Fluorometric)
MMP Activity Assay Kit (Fluorometric - Green)
MMP Activity Assay Kit (Fluorometric - Red)
MMP-1 Inhibitor Screening Kit
MMP-3 Activity Assay Kit (Fluorometric - Green)
MMP-3 Activity Assay Kit (Fluorometric)
TC supernatants, urine, plasma, serum
Cell lysate, milk, other biological fluids
Cell lysate, culture media, urine, plasma, serum
Pharmacological compounds
Urine
Cell lysate, urine, plasma, serum
Adherent cells, suspension cells
Adherent cells, suspension cells, cell culture extracts
Adherent cells, suspension cells, cell culture extracts
Compound inhibitors
Adherent cells, suspension cells, cell culture extracts
TC supernatants, cell culture extracts, tissue,
urine, plasma, serum
Compound inhibitors
protein-containing samples
TC supernatants, urine, plasma, serum
Neutrophil Elastase Inhibitor Screening Kit
Protein Carbonyl Content Assay Kit
Trypsin Activity Assay Kit (Colorimetric)
30
A/F
A/F
F
F
F
A
F
F
F
F
F
F
ab83387
ab107922
ab65357
ab133072
ab133084
ab111748
ab112142
ab112146
ab112147
ab118973
ab112148
ab118972
F
A
A
ab118971
ab126287
ab102531
Resources: www.abcam.com/metabolismkits
MMP Activity Assay Kit (Fluorometric – Green) (ab112146)
P
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Matrix metalloproteinases (MMPs) constitute a family of zinc-dependent
endopeptidases that function within the extracellular matrix and are responsible
for the breakdown of connective tissue. While the exact contribution of MMPs to
certain pathological processes is difficult to assess, MMPs play a key role in the
development of arthritis as well as in the invasion and metastasis of cancer.
This kit uses a FRET (fluorescence resonance energy transfer) peptide as a
generic MMP activity indicator, which allows the user to detect the activity of
any MMP enzyme and to screen MMP inhibitors.
Detection of MMP activity using ab112146. The APMA-activated MMPs (30 ng each) were mixed with a MMP green
substrate. The fluorescence signal was monitored one hour after starting the reaction by using a microplate reader
(Ex/Em = 490/525 nm). The reading of the substrate control (which contains the green substrate but no MMPs) was
subtracted from all wells. IN this case, different MMPs show different cleavage rate on this MMP substrate.
Metabolism
We have
MMP proteins too!
Resources: www.abcam.com/metabolismkits
31
MitoSciences
MitoSciences is a premium range of research tools designed
to advance mitochondrial research. MitoSciences assays
employ novel immunocapture based methods to drive the
advancement of mitochondrial research using monoclonal
antibodies target-validated by mass spectrometry and
characterized for sensitivity and specificity. Additionally, the
MitoSciences product line includes cellular fractionation kits,
enzyme activity assays, lateral flow assays and cellular labeling
assays. Also included in this range are the MitoTox™ assays,
which allow analysis of mitochondrial toxicity following
treatment of drugs and toxins.
MitoSciences
Microplate assay kits
Product
name
Sample
type
Aconitase Enzyme Activity Microplate Assay Kit
ATP synthase Enzyme Activity Microplate Assay Kit
Cell culture extracts, Tissue
Cell culture extracts, Tissue, Suspension cells,
Tissue Extracts, Cell Lysate
Cell culture supernatant, Cell culture extracts, Tissue,
Tissue Extracts, Cell Lysate
Cell culture extracts, Tissue
Mitochondria, tissue homogenate or cell lysate
Mitochondria, tissue homogenate or cell lysate
ATP synthase Specific Activity Microplate Assay Kit
Complex I Enzyme Activity Microplate Assay Kit
Complex II Enzyme Activity Microplate Assay Kit
Complex IV Human Enzyme Activity Microplate
Assay Kit
Complex IV Human Specific Activity Microplate
Assay Kit
Complex IV Rodent Enzyme Activity Microplate
Assay Kit
Cytochrome c Protein Quantity Microplate Assay Kit
Frataxin Protein Quantity Microplate Assay Kit
MitoTox™ Complex I OXPHOS Activity
Microplate Assay
MitoTox™ Complex II OXPHOS Activity
Microplate Assay
MitoTox™ Complex III OXPHOS Activity
Microplate Assay
MitoTox™ Complex IV OXPHOS Activity
Microplate Assay
MitoTox™ Complex V OXPHOS Activity
Microplate Assay
Pyruvate dehydrogenase (PDH) Combo
(Activity + Quantity) Microplate Assay Kit
Pyruvate dehydrogenase (PDH) Enzyme
Activity Microplate Assay Kit
Pyruvate dehydrogenase (PDH) Protein
Quantity Microplate Assay Kit
Detection
Product
code
A
A
109712
109714
A
ab109716
A
A
A
ab109721
ab109908
ab109909
Mitochondria, tissue homogenate or cell lysate
A
ab109910
Mitochondria, tissue homogenate or cell lysate
A
ab109911
Cell culture extracts, Tissue
Cell culture extracts, Tissue
Mitochondria
A
A
A
ab110172
ab110173
ab109903
Mitochondria
A
ab109904
Mitochondria
A
ab109905
Mitochondria
A
ab109906
Mitochondria
A
ab109907
Cell culture extracts, Tissue
A/C
ab110671
Cell culture extracts, Tissue
A/C
ab109902
Cell culture extracts, Tissue
A/C
ab110174
Detection
Product
code
DR/SC
DR/SC
DR/SC
DR/SC
DR/SC
DR/SC
DR/SC
DR/SC
ab109720
ab109722
ab109875
ab109876
ab109877
ab109878
ab109881
ab109880
Dipstick assay kits
32
Product
name
Sample
type
Complex I Enzyme Activity Dipstick Assay Kit
Complex I Human Protein Quantity Dipstick Assay Kit
Complex I Rodent Protein Quantity Dipstick Assay Kit
Complex IV Human Enzyme Activity Dipstick Assay Kit
Complex IV Human Protein Quantity Dipstick Assay Kit
Complex IV Rodent Enzyme Activity Dipstick Assay Kit
Frataxin Protein Quantity Dipstick Assay Kit
MetaPath™ Fatty Acid Oxidation 4-Plex Dipstick Array
Cell culture supernatant, Cell culture extracts, Tissue
Cell culture supernatant, Cell culture extracts, Tissue
Cell culture supernatant, Cell culture extracts, Tissue
Cell culture supernatant, Cell culture extracts, Tissue
Cell culture extracts, Tissue
Cell culture extracts, Tissue
Whole Blood
Cell culture extracts, Tissue
Resources: www.abcam.com/mitosciences
Dipstick assay kits (continued)
Product
name
Sample
type
Detection
Product
code
MetaPath™ Mito Disease 4-Plex Dipstick Array
Pyruvate dehydrogenase (PDH) Enzyme Activity
Dipstick Assay Kit
Pyruvate dehydrogenase (PDH) Protein Quantity
Dipstick Assay Kit
Cell culture extracts, Tissue
Cell culture extracts, Tissue
DR/SC
DR/SC
ab109879
ab109882
Cell culture extracts, Tissue
DR/SC
ab109883
Detection
Product
code
Cellular activity and staining kits
Product
name
Sample
type
CFSE - Cell Labeling Kit
DCFDA - Cellular Reactive Oxygen Species
Detection Assay Kit
JC1 - Mitochondrial Membrane Potential Assay Kit
Luminescent ATP Detection Assay Kit
Mitochondrial Viability Stain
TMRE—Mitochondrial Membrane Potential Assay Kit
Cells
Cells
F
F
ab113853
ab113851
Cells
Cells
Cells
Cells
F
L
F
F
ab113850
ab113849
ab129732
ab113852
Detection
Product
code
WB, dot blot, ELISA
WB, dot blot, ELISA
WB, immunoassay,
enzyme analysis
WB, immunoassay,
enzyme analysis
WB, immunoassay,
enzyme analysis
WB, immunoassay,
enzyme analysis
ab109718
ab109719
ab110168
Cell fractionation and mitochondrial isolation kits
Sample
type
Cell Fractionation Kit - HT
Cell Fractionation Kit Standard
Mitochondria Isolation Kit for Tissue
Cells
Cells / tissue
Tissue
Mitochondria Isolation Kit for Tissue
(with Dounce Homogenizer)
Mitochondria Isolation Kit for Cultured Cells
Tissue
Mitochondria Isolation Kit for Cultured Cells
(with Dounce Homogenizer)
Cells
Cells
MitoSciences
Product
name
ab110169
ab110170
ab110171
Stay up to date on
mitochondria and
metabolic research with
our MitoNews
Resources: www.abcam.com/mitosciences
33
Molecular Biology kits
Good starting material is the essential ingredient for good-quality experimental results.
Abcam’s isolation and preparation kits allow researchers to easily obtain the desired sub-cellular fraction for
their experiments. This extensive range includes kits ranging from enrichment of membrane proteins to
apoptotic DNA.
• Quick and easy protocols to follow
• Broad range of organelles and structures such as mitochondria, plasma membrane and viruses
Molecular Biology kits
Isolation and Preparation kit
Product
name
Sample
type
Applications/Detection
method
10kD Spin Column
Apoptotic Cell Enrichment Kit
Apoptotic DNA Ladder Isolation Kit
Apoptotic DNA Ladder Detection Kit
beta galactosidase Detection
Cytosol/Particulate Rapid Separation Kit
Apoptotic DNA Ladder Detection Kit (high sensitivity)
Genomic DNA Isolation Kit
Mammalian Cell Extraction Kit
Mitochondria/Cytosol Fractionation Kit
Mitochondrial DNA Isolation Kit
PEG Virus Precipitation Kit
Cell culture
Apoptotic cells, apoptotic tissue
Apoptotic cells
Culture cells
Cell culture, tissue
DNA from apoptotic tissue
Cell culture, tissue
Cell culture, tissue
Cell culture, tissue
Cell culture, tissue
Retroviruses, baculoviruses, lentiviruses, phages in
cell culture medium/environmental samples
Cell culture, tissue
Cellular or tissue lysates
Cells
Cells, tissue
Tissue
Tissue
sample preparation
extraction DNA fragments
detection DNA fragments on AG
Microscopy/Expression check
cellular fraction separation
detection DNA fragments on AG
Isolation of genomic DNA
cell lysate preparation
WB, ELISA
Isolation of mitochondrial DNA
virus concentration
ab93349
ab65782
ab65627
ab66090
ab102534
ab65398
ab66093
ab65358
ab65399
ab65320
ab65321
ab102538
WB, 2D gels, enzyme analysis
inhibition of proteases for cell lysates
WB, dot blot, ELISA
WB, dot blot, ELISA
WB, immunoassay, enzyme analysis
WB, immunoassay, enzyme analysis
ab65400
ab65621
ab109718
ab109719
ab110168
ab110169
Cells
Cells
WB, immunoassay, enzyme analysis
WB, immunoassay, enzyme analysis
ab110170
ab110171
Plasma Membrane Protein Extraction Kit
Protease Inhibitor Cocktail Formulation (DMSO)
Cell Fractionation Kit - HT
Cell Fractionation Kit Standard
Mitochondria Isolation Kit for Tissue
Mitochondria Isolation Kit for Tissue
(with Dounce Homogenizer)
Mitochondria Isolation Kit for Cultured Cells
Mitochondria Isolation Kit for Cultured Cells
(with Dounce Homogenizer)
MitoSciences Cell Fractionation Kit
Standard (ab109719)
Product
code
P
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Jurkat, HeLa and 143B osteosarcoma cells were treated with Fas and staurosporine respectively
to induce apoptosis, leading to cytochrome c release. Mitochondrial and cytoplasmic fractions
were isolated using ab109719 and probed with ApoTrack™ Cytochrome c Apoptosis WB
antibody Cocktail (ab110415) – GAPDH is used as cytoplasmic marker, whereas PDH E1 and
CV (ATP synthase subunit alpha) are used as mitochondrial markers.
Protein quantification kits
34
Product
name
Sample
type
BCA Protein Quantification
Protease Inhibitor Cocktail Formulation (DMSO)
Protein Quantitation Kit (Bradford Assay)
Protein Carbonyl Content Assay Kit
Cell lysate, tissue lysates, serum, plasma
Cell lysate, tissue lysates
Cell lysate, tissue lysates
Protein-containing samples
Product
code
ab102536
ab65621
ab102535
ab126287
Resources: www.abcam.com/molbiokits
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All information correct at time of printing.
Scientific support: www.abcam.com/technical
: MitoSciences product
35
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Technical Support: www.abcam.com/technical
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Resources: www.abcam.com
Local offices and distributors
1. Argentina
8. Kingdom of Saudi Arabia
17. Qatar
Tecnolab S.A.
Phone: +54-11-4555-0010
Email: info@tecnolab.com.ar
Web: www.tecnolab.com.ar
Abdulla Fouad Holding Co.
Phone: +966 9200 12100
Fax: +966 3 810 1644
Email: msd@abdulla-fouad.com
Web: www.abdulla-fouad.com
Sedeer Medical
Phone: +974 44885218
Fax: +974 44881988
Email: info@sedeer.com
Web: www.sedeer.com
2. Australia
Sapphire Bioscience Pty Ltd
Phone: 1800 062 088
/+61 (0) 2 9698 2022
Fax: +61(0) 2 9698 1022
Email: sales@sapphirebioscience.com
Web: www.sapphirebioscience.com
9. Korea, Republic of
18. Russia
Dawinbio Inc.
Phone: +82 (0)31 588 9741
Fax: +82 (0)31 790 4360
Email: dawinbio@dawinbio.com
Web: www.darwinbio.com
Almabion Ltd
Phone: +7 (8) 4732 779825
Fax: +7 (8) 4732 779825
Email: info@almabion.ru
Web: www.almabion.ru
3. Brazil
10. Kuwait
19. South Africa
Biogen Comercial e
Distribuidora Ltda
Phone: +55 11 3819 3191
Email: biogen@biogenbr.com.br
Web: www.biogenbr.com.br
BADER SULTAN & BROTHERS CO W.L.L
Phone: +965 24610480-7
Fax: +965 24610488/9
Email: info@sultan.com.kw
Web: www.badersultan.com
BIOCOM Biotech
Phone/Fax: +27 12 654 4614
Fax: +27 86 654 9048 (SA only)
Email: info@biocombiotech.co.za
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Biosonda S.A.
Phone: +56 2 209-6770
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Numelab s.a.r.l.
Phone: +961 (1) 39 66 77
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Biomed Diagnostics (Thailand) Co.,Ltd.
Phone: +66 (0)2 879 6026
Fax: +66 (0)2 879 6065
Email: nan@biomedthai.com
Web: www.biomedthai.com
12. Malaysia
5. Egypt
Bita Lifescience Sdn Bhd
Phone: +603-79608286
Fax: +603-79608262
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Web: www.bitalifesc.com.my
6a. India (East, South and West)
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Web: alliedscientific.net
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Web: www.ctr.com.mx
6b. India (North)
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NuLife Consultants &
Distributors (Pvt.) Ltd.
Phone: +91 (0)11 46112691/ 46112692/
29836141
Fax: +91 (0)11 46586216
Email: nulife@bol.net.in, sales@nulife.in
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Local offices and distributors
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Interlab Co., Ltd
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KiMERA Tıbbi Laboratuar Malzemeleri
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15. Oman
Bio Medical Scientific Services LLC.
Phone: +971 3 76 2 0880
Fax: +971 3 76 2 0882
Email: bmss.sale@gmail.com
Web: www.bmss-uae.com
16. Pakistan
24. United Arab Emirate
Bio Medical Scientific Services LLC.
Phone: +971 3 76 10070
Fax: +971 3 76 10080
Mobile: +971 50 449 09 39
Email: bmss.sale@gmail.com
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Friends Scientific Corporation
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Scientific support: www.abcam.com/technical
37
470_12_MF
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