Takara Bio Inc. Seta 3-4-1, Otsu, Shiga 520-2193, Japan www.takara-bio.com Certificate of Analysis Product name/description : Premix Ex Taq™ (Probe qPCR) Package Contents : 40 reactions 1. Premix Ex Taq (Probe qPCR) (2X conc.) 1ml Code No : RR390Q Lot No : AK102 2. ROX Reference Dye (50X conc.) 40 μl Storage Condition : 4 degrees C * 3. ROX Reference Dye II (50X conc.) 40 μl Shipping Condition : -20 degrees C Expiration Date : October 2012 * : This product can be stored at -20°C for long term storage. Once thawed, store at 4°C and use up within 6 months. Stable at 4°C for 6 months. Quality Control Data : PCR test : Consistent amplification results were observed in real time PCR using lambda DNA as a template (amplified fragment : 300 bp). Non-specific amplification was not detected in the reaction without template. It is certified that this product meets above specifications. Approved By : Minoru Ueda Manager, Quality Assurance TAKARA BIO INC. Page | 1 Premix Ex Taq™ (Probe qPCR) RR390Q Limited Use Label License & Patent Marking : [P7] PCR Notice : A license to perform the patented 5' Nuclease Process for research is obtained by the purchase of (i) both Authorized 5' Nuclease Core Kit and Licensed Probe, (ii) a Licensed 5' Nuclease Kit, or (iii) license rights from Applied Biosystems. This product is an Authorized 5' Nuclease Core Kit. Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155, 5,677,152, 5,773,258, 5,407,800, 5,322,770, 5,310,652, 5,210,015, 5,487,972, and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. Separate purchase of a Licensed Probe would convey rights under the applicable claims of US Patents Nos. 5,538,848, 5,723,591, 5,876,930, 6,030,787, 6,258,569, 5,804,375 (claims 1-12 only), and 6,214,979, and corresponding claims outside the United States. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained from the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. [L15] Hot Start PCR : Licensed under U.S. Patent No. 5,338,671 and 5,587,287 and corresponding patents in other countries. [L52] Rox Reference Dye (Research Field) : Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,928,907. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. [M40] Thermostable RNaseH : This product is covered by the claims of U.S. Patent No. 7,422,888 and its foreign counterpart patent claims. [M57] LA Technology : This product is covered by the claims 6-16 of U.S. Patent No. 5,436,149 and its foreign counterpart patent claims. [M82] Tli RNaseH Plus : This product is the subject of the pending JP patent application. This product may be covered by licenses. Visit the product page for this product on our website to get the most updated information. Safety Information : Please refer to our website (*) for safety information : (*)Japanese customers : http://www.takara-bio.co.jp Other country’s customers : http://www.takara-bio.us Notice To Purchaser : This product is intended to be used for research purpose only. They are not to be used for drug or diagnostic purposes, nor are they intended for human use. They shall not to be used products as food, cosmetics, or utensils, etc. Takara products may not be resold or transferred, modified for resale or transfer, or used to manufacture commercial products without written approval from TAKARA BIO INC. If you require licenses for other use, please call at +81 77 543 7247 or contact from our website. (*)Japanese customers : http://www.takara-bio.co.jp Other country’s customers : http://www.takara-bio.com or http://www.takara-bio.us Country of origin : China Page | 2 v201105