State Plant Breeding Institute
Wheat - showing Septoria tritici infection
Revealing multiple-disease resistance QTL in two
European wheat populations by meta-analysis
P. Risser1, S. Paillard2, T. Schnurbusch2, B. Keller2, J. Holzapfel3, L. Hartl3, H.F. Utz1, T. Miedaner1*
Introduction
Results
Septoria leaf blotch (STB, caused by Septoria tritici),
Fusarium head blight (FHB, caused by Fusarium
graminearum), and Stagnospora glume blotch (SGB,
caused by Stagnospora nodorum) are major diseases of
wheat (Triticum aestivum L.).
Common QTL regions for multiple-disease resistance
were located on chromosomes (i) 2B, 4D, 5B, and 7B in
H x R across STB and FHB and (ii) 3B, 4B, 5B, and 6D
in A x F across STB, FHB, and SGB (Figure 1).
Septoria leaf blotch (STB) 1
Fusarium head blight (FHB) [2]
Stagnospora glume blotch (SGB) [3]
Heading date (HED) 1
Plant height (PLH) 1
0.73
0.92
0.81
0 88
0.88
0.96
5
7
3
2
11
33.2
48.0
34.3
16 5
16.5
60.0
Literature:
[1] Holzapfel J, Voss H-H, Miedaner T, Korzun V, Häberle J, Schweizer G, Mohler V,
Zimmermann G, Hartl L (2008) Inheritance of resistance to Fusarium head blight in
three European winter wheat populations. Theor Appl Genet 117:1119-1128
[2] Paillard S, Schnurbusch T, Tiwari R, Messmer M, Winzeler M, Keller B,
Schachermayr G (2004) QTL analysis of resistance to Fusarium head blight in Swiss
winter wheat (Triticum aestivum L.). Theor Appl Genet 109:323-332
[3] Schnurbusch T, Paillard S, Fossati D, Messmer M, Schachermayr G, Winzeler M,
Keller B (2003) Detection of QTLs for Stagonospora glume blotch resistance in Swiss
winter wheat. Theor Appl Genet 107:1226-1234
[4] Utz HF, Melchinger AE (1996) PLABQTL: A program for composite interval mapping
of QTL. J Quant Trait Loci 2:1-5 (with extension for QTL meta-analysis, Utz 2009,
personal communication)
*Contact: Universität Hohenheim
State Plant Breeding Institute (720)
www.uni-hohenheim.de/lsa
miedaner@uni-hohenheim.de
Authors:
2
140
160
180
Xgwm767
25
FHB
(QFhs.fal-5BL.1)
SGB
(QSng.sfr-5BL)
PLH
20
10
8
120
200
Genetic distance on chr. 7B in cM
SGB
(QSng.sfr-4BL)
STB
12
100
XwPt-4057
80
Xgwm146
Xgwm344
60
Xgwm46
Xgwm43
XP6653-115
XP7661-276
Xgwm400
Xgwm636
Xgwm263
XwPt-8660
14
FHB + SGB
+ STB
FHB
SGB
STB
PLH
HED
LOD 6.0
LOD 3.0
Support interval
and position
of meta QTL
15
PLH
6
STB
(QStb.lsa_af-5B)
10
4
5
2
0
0
0 10 20 30 40 50 60 70
Genetic distance on chr. 4B in cM
0 10 20 30 40 50 60 70
Genetic distance on chr. 5B in cM
Figure 1: Illustration of meta QTL on chromosome 7B in History x Rubens and on
chromosomes 4B and 5B in Arina x Forno for multiple-disease resistance against
STB, FHB, and SGB. LOD curves for PLH and HED are given in comparison to
resistance traits. Rectangles with line insight show support interval and position
of meta QTL with initial QTL designation in brackets.
Conclusions
• Eight meta QTL with significant effects across three
pathogens were detected in two wheat populations
• Position, support interval, and closely linked
marker allow the implementation in marker assisted
breeding programs
• Further research is required to validate meta QTL
Universität Hohenheim
Universität Zürich
3 Bayerische Landesanstalt für Landwirtschaft
1
40
Xgwm639a
Xpsr120a
3 (1)
6 (3)
5 (2)
3 (1)
4 (1)
20
Xpsr574
54.8
57.8
50.0
68.4
0
Xgwm371
5
6
5
5
0
Xpsr945b
Xgwm274
0.87
0.93
0.93
0.98
5
Xgwm234
6 (2)
5 (2)
5 (2)
6 (2)
FHB
10
Xgwm443a
Septoria leaf blotch (STB)1
Fusarium head blight (FHB) [1]
Heading date (HED) 1
Plant height (PLH) 1
Arina x Forno (N = 200)
h² No. of QTL Total R²adj
(LOD ≥ 3.0)
3 0)
(%)
15
Xglk348a
No. env. (years)
Trait (Abbreviation) [data source]
History x Rubens (N = 94)
20
Xglk335
Xgwm538
Population (size)
25
Xgwm368
Table 1: Mapping populations History x Rubens and Arina x Forno with number of
environments (No. env.), years, and heritabilities (h²) for evaluated traits in field
trials and total adjusted phenotypic variance (Total R²adj) explained by detected
QTL with LOD ≥ 3.0 in initial QTL-analysis performed with PLABQTL.
FHB
STB
(QStb.lsa_hr-7B)
30
Xgwm165
Xpsr914
QTL meta-analysis was conducted with PLABMQTL [4]
based on high-density linkage maps of segregating
populations History x Rubens (H x R) and Arina x Forno
(A x F). Raw data of phenotypic evaluations from
multienvironmental field trials (Table 1) were used in
composite interval mapping to get comparable LODcurves across different experiments and diseases. Metaanalysis was done by adding LOD-scores of disease
traits within H x R and A x F to reveal p
position,, support
pp
interval, and additive effects significant for minimum two
disease traits with an overall LOD ≥ 6.0.
LOD History x Rubens
Material and methods
35
LOD Arina x Forno
The objective of this study was to reveal common
QTL regions for multiple disease resistance to FHB,
STB, and SGB in wheat by meta-analysis.
Funding: ERA-NET Plant Genomics “CEREHEALTH”
Cooperations: Bayerische Landesanstalt für Landwirtschaft
KWS LOCHOW GMBH