CH927 QTL mapping practical write up 2013
QTL mapping of glucosinolate production in Arabidopsis
Write-up for practical
You’ll write this practical up as if you had carried out this work and will need to
include the following sections:
Introduction
A brief introduction (2 pages max)

Explain the principle of QTL mapping and how it can be used in
agriculture to find genes that control phenotypes (traits) of interest for
crop improvement (find some examples of this)

-Explain why glucosinolates are a trait of interest to study
Methods
A brief methods section (2 pages max)

Explain what the genotype and phenotype data that you used was,
and where it came from (refer to the Wentzell et al paper)
Results and discussion section:

What type of information can you find out about the QTLs that are
detected in the analysis that you carried out?

Why might the QTLs that you detect be different when you use
different QTL mapping methods (Haley-Knott etc).
(2 pages max)
Plot an eQTL LOD for AOP3.

What does this plot tell you, explain what it shows in detail.

Are there cis or trans (or both) effects?

Which genes are predicted to regulate AOP3 expression (identify the
genes from the LOD plots and use www.arabidopsis.org (TAIR) to
search for information on them.

List all of the genes that you think might control AOP3 and suggest
which ones you think are most likely
(2 pages max).