Mary Williard Elting University of California, San Francisco, Department of Cell and Tissue Biology 513 Parnassus Ave., HSW 601, San Francisco, CA 94143-­‐0512 (415) 502-­‐1236 (lab) mary.elting@ucsf.edu EDUCATION & RESEARCH EXPERIENCE University of California, San Francisco Sept. 2012-­‐present Postdoctoral Fellow with Sophie Dumont, Department of Cell and Tissue Biology Damon Runyon Cancer Foundation Postdoctoral Fellow Research: Mechanical design principles of spindle architecture for robust chromosome segregation Stanford University Sept. 2006-­‐May 2012 Graduate research with James Spudich, Department of Biochemistry NSF Graduate Research Fellow Ph. D., Applied Physics, Sept. 2012 MS. Applied Physics, Jan. 2009 Thesis: Probing mechanisms of myosin motors using single molecule techniques North Carolina State University Aug. 2003-­‐Jun. 2006 Park Scholar, Goldwater Scholar B.S., Physics, Minor in French, summa cum laude, May 2006 Undergraduate research with Keith Weninger, Department of Physics Research: Single-­‐particle fluorescence for testing mechanism of Sindbis virus infection INDEPENDENT FUNDING AND AWARDS Postdoctoral June 2015 Best Poster Award, FASEB Research Conference on Mitosis: Spindle Assembly and Function Sept. 2013-­‐2017 Damon Runyon Cancer Foundation Postdoctoral Fellowship: Includes four years of postdoctoral salary and an annual research/travel budget 2013 Jane Coffin Childs Postdoctoral Fellowship, declined 2013 American Heart Association Postdoctoral Fellowship, declined Graduate 2011-­‐2012 Lieberman Fellowship: Awarded to three senior graduate students in the Stanford School of Humanities and Sciences per year, based on criteria of research, teaching, and academic leadership 2009-­‐2010 ARCS Scholarship: Award to support science Ph.D. students 2006-­‐2009 National Science Foundation Graduate Research Fellowship Undergraduate 2005-­‐2006 Goldwater Scholarship: National scholarship awarded to junior and senior undergraduates interested in careers in research 2003-­‐2006 Park Scholarship: Includes all cost of attendance for four years at NCSU; awarded based on criteria of scholarship, leadership, service, and character May 2006 NCSU Physics Department: McCormick Award for Undergraduate Research; Outstanding Graduating Senior Award for Research; Outstanding Graduating Senior Award for Scholarly Achievement 2004 NCSU Honors Program Undergraduate Award for Creative and Research Projects: Obtained summer funding for research on Sindbis virus conducted in the laboratory of Keith Weninger 2003 National Merit Scholarship: Academic competition based on PSAT scores CV 2/4 Mary Williard Elting RESEARCH Publications § MW Elting*, CL Hueschen*, DB Udy, S Dumont. Force on spindle microtubule minus ends moves chromosomes. Journal of Cell Biology 206(2):245-­‐56 (2014). Commentaries on JCB Biobytes Podcast and F1000. § MW Elting, SR Leslie, LS Churchman, J Korlach, CMJ McFaul, JS Leith, MJ Levene, AE Cohen, JA Spudich. Single-­‐molecule fluorescence imaging of processive myosin with enhanced background suppression using linear Zero-­‐Mode Waveguides (ZMWs) and Convex Lens Induced Confinement (CLIC). Optics Express. 21(1): 1189-­‐1202 (2013). § MW Elting, JA Spudich. Future challenges in single-­‐molecule fluorescence and laser trap approaches to studies of molecular motors. Developmental Cell. 23(6): 1084-­‐1091 (2012). § MW Elting, Z Bryant, JC Liao, JA Spudich. Detailed tuning of structure and intramolecular communication are dispensable for processive motion of myosin VI. Biophysical Journal. 100(2): 430-­‐439 (2011). § JC Liao, MW Elting, SL Delp, JA Spudich, Z Bryant. Engineered myosin VI motors reveal minimal structural determinants of directionality and processivity. Journal of Molecular Biology. 392: 862-­‐867 (2009). § L Wessels, MW Elting, D Scimeca, K Weninger. Rapid membrane fusion of individual virus particles with supported lipid bilayers. Biophysical Journal. 93(2): 526-­‐538 (2007). Manuscripts in preparation § MW Elting, DB Udy, S Dumont. Local anchorage of kinetochore-­‐fibers to the mammalian spindle provides mechanical isolation and load-­‐bearing redundancy. Manuscript in preparation. § MW Elting, S Dumont. Mechanics and architecture of the mammalian spindle shape cell division. Invited review in preparation for Trends in Cell Biology. § T Karg, MW Elting, S Dumont, W Sullivan. The chromokinesin klp3a and microtubules drive acentric chromosome segregation. Manuscript in preparation. Invited Talks § How do kinetochore microtubules anchor to the mitotic spindle? Chromatin Club, UC Santa Cruz, Santa Cruz, CA (April 2015). § Probing mechanisms of myosin molecular motors using single molecule techniques. inStem Seminar Series, National Center for Biological Sciences, Bangalore, India (August 2012). § Probing mechanisms of myosin molecular motors using single molecule techniques. Department of Physics, McGill University, Montreal, QC, Canada (July 2012). Talks § MW Elting, DB Udy, S Dumont. Local anchorage of kinetochore-­‐fibers to the mammalian spindle provides mechanical isolation and load-­‐bearing redundancy. Biophysical Society Annual Meeting, Los Angeles, CA (accepted for presentation March 2016). § MW Elting. Kinetochore-­‐fibers are anchored locally in the mammalian spindle through neighboring microtubules. Damon Runyon Cancer Research Foundation Fellows’ Retreat, San Jose, CA (September 2015). § MW Elting, CL Hueschen, DB Udy, S Dumont. Force on spindle microtubule minus ends moves chromosomes. American Society for Cell Biology Annual Meeting, Philadelphia, PA (December 2014). § MW Elting, CL Hueschen, DB Udy, S Dumont. Rapid detection and poleward transport of new microtubule minus-­‐ends by NuMA and dynein maintain spindle organization. UCSF Research in Progress Seminar, San Francisco, CA (November 2013). § MW Elting. Probing mechanisms of myosin molecular motors using single molecule techniques. Thesis Defense, Department of Applied Physics, Stanford University, Stanford, CA (June 2012). § MW Elting, Z Bryant, JC Liao, JA Spudich. Tuning of structure and intramolecular communication are not required for myosin VI processivity. Gordon Research Conference on Muscle and Molecular Motors, New London, NH (July 2011). § MW Elting, Z Bryant, JC Liao, JA Spudich. Tuning of structure and intramolecular communication are not necessary for myosin VI processivity. Department of Biochemistry Annual Retreat, Stanford University, Fallen Leaf Lake, CA (October 2010). § MW Elting, Z Bryant, JC Liao, JA Spudich. Probing myosin VI processivity using artificial lever arms. Single Molecule Biophysics Meeting, Aspen, CO (January 2009). § MW Elting, Z Bryant, JC Liao, JA Spudich. Impact of artificial lever arms on myosin VI processivity. Biophysical Society Annual Meeting, Long Beach, CA (February 2008). CV 3/4 Mary Williard Elting § M Williard, G Wang, K Weninger. Visualizing viral infection with single particle fluorescence. McCormick Award for Undergraduate Research in Physics, North Carolina State University, Raleigh, NC (May 2006). § M Williard, G Wang, K Weninger. Visualizing interactions between Sindbis virus and cells by single particle tracking. American Physical Society March Meeting, Los Angeles, CA (March 2005). Selected Poster Presentations § MW Elting, DB Udy, S Dumont. Local anchorage of kinetochore-­‐fibers to the mammalian spindle provides mechanical isolation and load-­‐bearing redundancy. American Society for Cell Biology Annual Meeting, San Diego, CA (December 2015). § MW Elting, DB Udy, S Dumont. Probing anchorage of kinetochore-­‐fibers within the mammalian spindle via laser ablation. FASEB Research Conference on Mitosis: Spindle Assembly and Function, Big Sky, MT (June 2015). Winner of Best Poster Award. § MW Elting, CL Hueschen, DB Udy, S Dumont. Using laser ablation to probe how the mammalian spindle anchors its kinetochore-­‐fibers. Bay Area Centromere and Chromosome Dynamics Meeting, Santa Cruz, CA (January 2015). § MW Elting*, CL Hueschen*, DB Udy, S Dumont. Force on spindle microtubule minus ends moves chromosomes. American Society for Cell Biology Annual Meeting, Philadelphia, PA (December 2014). § MW Elting, CL Hueschen, DB Udy, S Dumont. Probing k-­‐fiber anchorage in the mitotic spindle via laser ablation. Bio-­‐X Symposium on Mechanobiology: Pushing and Pulling on Life, Stanford University, Stanford, CA (November 2014). § MW Elting*, CL Hueschen*, DB Udy, S Dumont. Forces generated at spindle minus-­‐ends move chromosomes. American Society for Cell Biology Annual Meeting, New Orleans, LA (December 2013). § MW Elting, CL Hueschen, DB Udy, S Dumont. Probing forces on newly generated kinetochore-­‐fiber minus-­‐
ends. Gordon Research Conference on Motile and Contractile Systems, New London, NH (July 2013). § MW Elting, SR Leslie, LS Churchman, J Korlach, CMJ McFaul, JS Leith, MJ Levene, AE Cohen, JA Spudich. Techniques for reducing background fluorescence for single molecule imaging of processive myosins: linear Zero Mode Waveguides (ZMW) and Convex Lens Induced Confinement (CLIC). American Society for Cell Biology Annual Meeting, San Francisco, CA (December 2012). § MW Elting, Z Bryant, JC Liao, JA Spudich. Probing myosin VI processivity using artificial lever arms. Biophysical Society Annual Meeting, San Francisco, CA (February 2010). § MW Elting, Z Bryant, JC Liao, JA Spudich. Structural and mechanistic determinants of myosin VI processivity. American Society of Cell Biology Annual Meeting, San Francisco, CA (December 2008). § M Williard, G Wang, K Weninger. Visualizing interactions between Sindbis virus and cells by single particle tracking. North Carolina State University Undergraduate Research Symposium (2006). Professional societies and honors § Biophysical Society (BPS) § American Society for Cell Biology (ASCB) § Sigma Pi Sigma (national physics honors society) § Phi Beta Kappa TEACHING AND SERVICE Teaching experience Fall 2011 Guest lecturer for Performance, Development, and Adaptation of Skeletal Muscle (advanced undergraduate/graduate student course), taught by Melinda Cromie and Holly Liske, Bioengineering, Stanford University. Spring 2011 Teaching assistant for Interdisciplinary Approaches to Biochemistry: Single Molecule Biophysics to Clinical Outcomes (upper-­‐level graduate course), taught by James Spudich, Biochemistry, Stanford University. Winter 2011 Discussion session teaching assistant for Mechanics (introductory undergraduate series), taught by Sarah Church, Physics, Stanford University. Summer 2010 Guest lecturer for Physics of Light and Heat (introductory undergraduate series), taught by Chaya Nanavati, Physics, Stanford University. Fall 2009 Laboratory teaching assistant for Physics of Light and Heat (introductory undergraduate series), taught by Doug Osheroff, Physics, Stanford University. Fall 2008 Teaching assistant for Molecular and Cellular Bioengineering (first-­‐year graduate student course), taught by Zev Bryant, Bioengineering, Stanford University. CV 4/4 Mary Williard Elting 2004-­‐2006 Teaching training 2012 2011 2009 Mentoring 2013-­‐2016 Summer 2010 2009-­‐2010 Tutor/grader for five undergraduate Physics-­‐major courses, North Carolina State University. Teaching Workshop for Scientists, Science and Health Education Partnership, UCSF. Science and Engineering Course Design, Stanford University. Studied concepts of science course design, and, over the course of the entire class, prepared a detailed syllabus for an introductory biophysics course. Teaching of Physics Seminar, Stanford University. Mentor for four UCSF graduate program rotation students in the Dumont lab. Mentor for one undergraduate student from Hampton University during Stanford Summer Research Program-­‐Amgen Scholars Program. Mentor for two Stanford graduate program rotation students in the Spudich lab. Service 2013 2009-­‐2012 Apr. 2009, 2010 Jan. 2007 Teacher through Science and Health Education Partnership, UCSF. Partnership with one other UCSF scientist and a San Francisco middle school science teacher at Aptos Middle School. Tutor for Science Bus. Stanford graduate students lead lessons at East Palo Alto Charter School to encourage interest in and enthusiasm for science with elementary school students. Splash! Instructor. Local middle and high school students come to campus for one day of classes taught by Stanford students. Judge of Science Fair at Terman Middle School.