Instruction Manual
Model 125 Electrophoresis
Power Supply
CAT. SERIES 31061
Table of Contents
1. Introduction .................................................. 1
1.1 Notice to Customer ................................ 1
1.2 Warnings ................................................ 1
2. Overview ....................................................... 3
2.1 Description ..............................................3
2.2 Set-Up .................................................... 5
3. Operating Instructions ................................ 7
4. Troubleshooting Guide ............................. 10
5. Related Products ....................................... 12
6. Additional Information............................... 13
6.1 Care and Handling ............................... 13
6.2 Specifications ....................................... 13
6.3 Warranty............................................... 14
6.4 Declaration of Conformity
and CE Mark .........................................15
Figures
1. Model 125 Electrophoresis
Power Supply .................................................4
2. Model 125 Rear and Bottom Views ............... 6
HORIZON®, SUNRISE™ , TECH-LINESM, and the Life Technologies
logo are marks of Life Technologies, Inc.
i
1
Introduction
1.1 Notice to Customer
This product is authorized for laboratory research
use only. The product has not been qualified or
found safe and effective for any human or animal
diagnostic or therapeutic application. Uses for
other than the labeled intended use may be a violation of applicable law.
If the product is used in a manner not specified by
the manufacturer, the protection provided by the
product may be impaired.
1.2 Warnings
1. DANGER! HIGH VOLTAGE! This power supply has been designed for use as a DC power
source for electrophoresis. It is capable of
generating lethal currents. This unit should
always be operated with extreme caution.
Careless handling could result in electrical
shock.
2. Never operate damaged equipment. Do not
open the power supply. There are no userserviceable parts inside. If the power supply
emits smoke or fails to operate, turn off the
unit and disconnect the AC line and power
cords.
3. Do not operate with DC power cords that have
exposed live wires.
1
4.
Always turn off the power supply before
connecting or removing the DC power cords
or moving the electrophoresis apparatus.
5. Handle one power cord at a time, with one
hand only, when connecting or removing the
DC power cords at the power output
terminals.
6. Do not immerse the power supply in water.
7. Do not operate the power supply in a damp,
humid atmosphere or in a fashion where
condensed moisture may short out electrical
components.
8. Do not connect the DC output to earth ground.
9. The AC power cord has a three-pronged plug
which must be connected to a grounded line
voltage receptacle. Do not use a two-wire
receptacle with an adapter. This could create
a
serious
electrical
hazard
for
persons using the power supply.
10. Use the correct AC line voltage for the power
supply. Be sure to always plug a 115 VAC
power supply into a 115 V line and use a 230
V line with a 230 VAC power supply. The AC
line voltage selector switch is located on the
bottom of the power supply.
2
2
Overview
2.1 Description
The Model 125 Electrophoresis Power Supply provides controlled DC output of 50, 100, and 125 V.
The power supply operates in constant voltage
mode, at currents up to 150 mA. The single set of
recessed output terminals allows for one electrophoresis apparatus to be run at a time. Safety
features include a PTC (Positive Thermal
Coefficient) device that automatically resets when
a fault condition is removed.
3
Power on indicator
Power switch
(On/Off)
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5
Output voltage
selector switch
Negative (-) output
Positive (+) output
Figure 1. Model 125 Electrophoresis Power Supply.
4
Overview
2.2 Set-Up
Unpacking the Power Supply. Unpack and
inspect the power supply carefully for any damage.
Do not use the power supply if it is damaged. If the
power supply is damaged, save the packing material and report the problem to Customer Service.
Location. Make sure the power supply is set up in
a location where it is protected from physical
damage, moisture, corrosive agents, and extreme
temperatures. The power supply should be readily
accessible for safe operation.
Connection with the AC Mains. Make sure the
line selector switch located on the bottom of the
unit (figure 2) is set to the correct line voltage
(115/230 VAC). Connect the power supply to the
AC mains with the correct line voltage. Make sure
the main receptacle and the power supply have the
proper three-wire (grounded) connections.
5
AC input receptacle
Fuse tray
Mains voltage
selection switch
Figure 2. Model 125 Rear and Bottom Views.
6
3
Operating Instructions
Read this section in its entirety before setting up or
operating the Model 125 Electrophoresis Power
Supply.
1. Place the Model 125 Power Supply, with the
electrophoresis apparatus, on a level surface
in a safe, dry, isolated area. When moving the
power supply from room temperature into a
cold room or vice versa, allow at least 8 h for
the power supply to equilibrate before use.
Note: Allow sufficient space for the electrophoresis apparatus to be connected with the
power supply. Place and orient the power supply in such a manner that the controls on the
front and top panels are easily accessible.
Post warning signs to indicate that high voltage is present.
7
2.
Make sure that the power switch located on the
top panel of the power supply is in the off position, indicated by the symbol “O” (figure 1).
3.
Place the AC power cord into the threepronged inlet receptacle located on the rear
panel (figure 2).
Note: The AC power cord has a threepronged plug. This must be connected to a
grounded line voltage receptacle. Do not use
a two-wire receptacle with an adapter. This
could create a serious electrical hazard for
persons using the power supply.
4.
Check to ensure that the line selector switch
located on the bottom of the power supply (figure 2) is set to the proper voltage (115/230
VAC).
5.
Plug the AC power cord into an outlet of the
proper line voltage.
Note: Do not plug the power supply set for
115-V into a 230-V line.
6.
Connect the Model 125 Power Supply to the
electrophoresis apparatus using the DC power
cords. The power cords are color-coded: the
black is negative, and the red is positive.
7.
Set the voltage selector (located on the front
panel, figure 1) to the desired output voltage
of 50, 100, or 125 VDC.
8.
Turn the power switch (located on the upper
left corner of the front panel, figure 1) to the
on position. The green power indicator light
will illuminate. The DC output is now on, and
electrophoresis starts in the apparatus
connected to the power supply.
9.
Determine that the power supply is working
properly by watching for tiny bubbles on the
electrodes in the buffer tray of the electrophoresis apparatus.
8
Operating
Instructions
10. When the electrophoresis run is complete, turn
the power switch to the off position.
11. Disconnect the Model 125 Power Supply from
the wall outlet.
12. Disconnect the power cords from the Model
125 Power Supply and the electrophoresis
apparatus.
9
Troubleshooting Guide
4
Many operating problems may be solved by carefully following the instructions in this manual. Some suggestions for troubleshooting are included
here. Should these suggestions not resolve the problem, the unit should be
returned to Life Technologies for repair. A full description of the problem
should be included.
Contact the Customer Service Department for shipping instructions.
Questions regarding procedures should be directed to the Life Technologies’
TECH-LINE . Customers outside of the United States should contact their
local distribution center for information regarding repairs.
SM
Problem
Comments
Power supply is not supplying
DC voltage upon start up
Verify that the AC power cord is plugged into a working outlet
of the proper voltage.
Verify that the line selector switch is set to the correct voltage.
Verify that the power switch is in the on position.
During operation, the green
light goes out
The Model 125 is equipped with a replaceable current fuse
(T0.5A/250V 5 x 20 mm for both 115/230 VAC). Disconnect
the power cord from the power supply before checking or
exchanging the fuse. The fuseholder tray is located beneath
the AC input receptacle on the rear panel of the power supply.
The fuseholder has two tabs which need to be gripped in order
to remove the fuse. If the fuse is blown (black spot), it needs to
be replaced. Insert the new fuse into the tray and push the
assembly back into the AC input module.
10
Troubleshooting
Guide
Problem
Comments
During operation, power supply
stops supplying DC voltage
(but the green light remains on)
If a fault condition , i.e., short-circuit or current overload,
occurs on the DC output, the PTC (Positive Temperature
Coefficient) device will activate and impede current flow.
Rotate the power (I/O) rocker switch to “off” position, correct
the fault condition, and allow the unit to cool down. The PTC
safety device will automatically reset to normal resistance
mode.
Line selector switch is not set
to local voltage
Slide the voltage selector switch to its proper position, exposing the desired voltage setting (115/230 VAC).
Positive or negative power cords
will not fit in banana jacks tightly
Verify that the banana jacks are the appropriate size.
11
5
Related Products
Product
Cat. No.
Apparatus:
HORIZON® 58 Horizontal Gel
Electrophoresis Apparatus
41060-013
SUNRISE™ Horizontal Electrophoresis 10245-017
Apparatus
HORIZON 11•14 Horizontal Gel
Electrophoresis Apparatus
11068-012
HORIZON 20•25 Horizontal Gel
Electrophoresis Apparatus
21069-018
Mini-V 8•10 Vertical Gel
Electrophoresis Apparatus
21078-092
Replacement Fuse, T0.5A/250V
11604-030
Reagents:
Agarose, 100 g
15510-019
Agarose, 500 g
15510-027
10X TBE Buffer, 1 L
15581-044
10X TBE Buffer, 4 L
15581-036
10X TAE Buffer, 1 L
15558-042
10X TAE Buffer, 4 L
15558-026
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6
Additional Information
6.1
Care and Handling
The Model 125 Electrophoresis Power Supply is
constructed from flame-retardant ABS plastic. The
power and voltage selector switches are made of
molded plastic.
The power supply must not be immersed in water.
The unit can be wiped with a soft cloth, dampened
with water and a nonabrasive, mild soap or detergent. Do not allow water to enter the case.
Abrasive cleaners, window sprays or rough cloths
may damage the surface and should be avoided.
Grease and oils may be removed using a light
application of hexane, kerosene or aliphatic naphtha. Do not expose the surface to phenol, acetone,
benzene, halogenated hydrocarbon solvents or
undiluted laboratory alcohols. Avoid prolonged
exposure of the power supply to UV light. A soft,
dry cloth may be used to dry the unit.
6.2
Specifications
“Installation Category II”
Net Weight:
Shipping Weight:
Dimensions (D x W x H)
0.8 kg (1.7 lb.)
0.85 kg (1.9 lb.)
12.1 x 7.6 x 5.7 cm
(4.7 x 3.0 x 2.2 in.)
Enclosure Construction:
Injection molded ABS plastic,
ultrasonically sealed
Power Requirement:
115/230 VAC, 50/60 Hz, 50 W
Fuses:
T0.5A/250V 5 x 20 mm for 115/230 VAC
Operating Temperature Range:
4°C - 30°C
(non-condensing atmosphere)
DC Output Voltage:
50, 100, 125 VDC
DC Output Current Range:
0-150 mA
AC Power Cord:
91.4 cm (36 in.)
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6.3
Warranty
Life Technologies, Inc. warrants apparatus of its
manufacture against defects in materials and
workmanship, under normal service, for one year
from the date of receipt by the purchaser. This
warranty excludes damages resulting from shipping, misuse, carelessness, or neglect. Life
Technologies’ liability under the warranty is limited
to the repair of such defects or the replacement of
the product, at its option, and is subject to receipt
of reasonable proof by the customer that the
defect is embraced within the terms of the warranty. All claims made under this warranty must be
presented to Life Technologies within one year following the date of delivery of the product to the
customer.
This warranty is in lieu of any other
warranties or guarantees, expressed or
implied, arising by law or otherwise. Life
Technologies makes no other warranty,
expressed or implied, including warranties of
merchantability or fitness for a particular
purpose. Under no circumstances shall Life
Technologies be liable for damages either
consequential, compensatory, incidental or
special, sounding in negligence, strict liability,
breach of warranty or any other theory, arising
out of the use of the product listed herein.
Life Technologies reserves the right to make
improvements in design, construction, and
appearance without notice.
14
Additional
Information
6.4 Declaration of Conformity and CE
Mark
Note: The information outlined in this section
applies only to customers located in the European
Union (EU). The EU is currently comprised of 15
member countries.
This laboratory apparatus is identified with the CE
mark. This mark indicates that the product complies to the following EU Directives and Standards:
Application of Council Directive(s):
89/336/EEC
Electromagnetic Compatibility
73/23/EEC
Low Voltage Directive
Standards:
EN 50081-1:1992
EN 50082-1:1992
EN 61010-1:1993
Emissions
Immunity
Product Safety
EU Representative:
Life Technologies Ltd.
EU Address:
3 Fountain Dr.
Inchinnan Business Park
Paisley, PA49RF Scotland
A copy of the Declaration of Conformity certificate
is available upon request.
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Part No.
Lot. No.
50508
NBWP01-0200