Anpan Han (Ph. D.)
Harvard University, Dept. Physics. 17 Oxford St.
Jefferson 166, Cambridge, MA-02138, USA
Phone: +1 617 495 8684
E-mail: ahan@ fas.harvard.edu
98 Hancock St. Apt. 2
Cambridge, MA-02139, USA
Phone: +1 617 717 9370
Personal information
Marital status:
Gender:
Single
Male
Nationality:
Date of Birth:
Danish
18th July, 1978
EDUCATION
IMT Universitéde Neuchâtel (Institute of Microengineering, EPFL, Switzerland), 2002-2006
Ph.D. in Micro Nanotechnology. Thesis title: Nanopores and Nanochannels – Fabrication and
Applications in Analytical Biochemistry and Colloidal Sciences.
Niels Bohr Institute, University of Copenhagen, Denmark, 1997 – 2002
M. Sc. in Biophysics, B. Sc, in Biophysics, B. Sc in Physics, cumulative average score 10.6, top 5%.
Residence at Nordisk Kollegium 1999-2002
RESEARCH INTERESTS and UNIQUE STRENGTH
Design and fabricate nano and mic rofluidic devices and sensors using micro- a nd
nanofabrication tools. Sc ientific studies a nd applications. Setting up laboratory. Instrument
design of e lectron mic roscopy, high vacuum and cryogenic systems.
Na nopore se nsor: a membrane with a single pore with a diameter less than 50 nm separates two
liquid reservoirs. Ionic current is measured thro ugh the pore, as a molecule translocates the pore, the
ionic current is perturbed and an electronic signature is recorded. Nanopores allow biomolecular
recognition on single molecule level, characterize single protein molecules, biochemical assays with
single molecule sensitivity.
Single carbon nanotube fie ld effect transistor devices: de novo rapid DNA sequencing combined
with nanopores. Biosensing applications.
Na nochanne l dev ice: fluidic flow dynamics in sub 30 nm channels, electrokinetic separation.
Microfluidic devices: uncoiling and random f ragmentation of DNA, sample preparation for shot -gun
DNA sequencing. Competitive immunoassays for in-vitro diagnostic applications
RESEARCH and WORK EXPERIENCE
Post doctorial fellow at the Harvard Nanopore Group, Harvard University. 2008 – now
Prof. Jene Golovchenko, Prof. Dan Branton. Rapid de novo electronic DNA sequencing using
nanopore devices with embedded carbon nanotube (CNT) electrodes in the pore lumen. As a single
stranded DNA is pulled through a pore of less than 10 nm in diameter by electrophoresis, the
embedded CNTs, acting as current tunnelling probes, read and distinguish the electrical signature of
each DNA base. I co-designed and constructed the first ice-lithography instrument system dedicated
for making single carbon nanotube field effect transistor (CNT FET) for sequencing devices. The
instrument consists of a scanning electron microscope (JEOL TFE7001F), a high vacuum metal sputter
deposition system, vibration insulated cryostages, and e-beam lithography system (NPGS, Nabity).
Successfully fabricated pristine CNT FET using instrument.
Ph.D. studies at IMT, Universitéde Neuchâtel, Switzerland (2002-2006)
Prof. Urs Staufe r and Prof. Nico de Rooij. First to make nanopores 20 nm in diameter on a wafer
scale. Established measurement setup. First to detect single protein molecules using nanopores and
determined their size w ith subnanometer acc uracy. First to investigate intermolecular interactions
(antibody-antigen binding) using nanopores. Developed new method to make very long nanochannels,
down to 30 nm deep, 200 nm wide and up to 10 c m long. Studied filling kinetics, electrokinetic flow
and electrokinetic separation in nanochannels. Supervised by Prof. Sabeth Ve rpoorte, I made
microf luidic devices to study the uncoiling and breaking of double stranded DNA molecules in
elongational and shear flow. Such devices have applications in sample preparation for DNA sequencing.
Immunoassay in microchannel systems.
Post doctorial fellow at DTU Nanotech, Tech. Uni. Denmark (2007 – 2008)
Prof. Ole Hanse n. Designed and fabricated AFM cantilever based electrical probes for advanced
metrology instruments for CMOS industry in collaboration w ith industrial partner Capres A/S. Using
fabricated probes we measured electrical sheet resistance measurement s of next generation CMOS
ultra shallow junction samples. Initiated and developed novel fabrication process for making micro and
nanostructures using thin-film diamond. Successfully fabricated diamond probes.
Technology Development Manager at Toxispot A/S, a spin-off DHI, Novi A/S (2006-2007)
Project management and development of miniaturized bio-sensors and a portable analytical instrument
for environment monitoring and early warning. Established c ollaborations with Danish and European
partners.
Master studies at Institute of Micro and Nanotech., DTU (2000 - 2002)
Prof. Martin Dufva. Developed an immunoassay on a microarray format to test for pesticide residues
in drinking water. Immobilizing proteins onto microarray and nanoparticles. Assay development and
testing.
SKILLS and METHODS













Device fabrication using mic ro and na nofa brication tools carrie d out in state -of-theart c leamroom facilities at Harvard Uni., Tech. Uni. of Denma rk, a nd EPFL Switze rla nd.
E-beam lithography (Elionix, Raith, Nabity on JEOL TFESEM), photolithography (Suss Microtec,
EVG), plasma etching (STS ICP systems), CVD (STS), wet chemical etching, thermal- and ebeam thin-film evaporation (Leybold, Alcatel, Sharon), ellipsometer, profilometer.
Design of nano and micro devices for nano and bioscience/technology applications
Ultra high vacuum (UHV) system design and implementation
Instrument design for cryogenic SEM applications
Ultra low noise current measurements using electrophysiology equipme nt
Biochemical assay development
Microscopy: light, fluorescence, AFM (DI, Asylum), SEM (LEO, FEI, JEOL, Zeiss, Philips).
DNA and protein microarray technology
Mechanical design (Solid Works) and machine workshop certificate
Patent applications
Project management
Application of public and private funding
Out-sourcing to Danish and European companies
RESEARCH SUPPORT
Internationalization Ph.D. Grant from the Danish Agency for Science, Technology and Innovation
(120000 Euro). Post doctoral fellowship Grant from the Carlsberg Foundation (90000 Euro).
TEACHING
Practical courses: Electron beam lithography and nano and microfabrication. Supervision:
Undergraduate projects
LANGUAGES
Chinese as mother tongue, fluent English and Danish. French and Swedish secondary.
UNIVERSITY SERVICES and INTERESTS
2009–2010 Elected Chair w ith the Harvard Chinese Student Scholar Association. 2007-2008
“Fredagsbar” initiator at DHI, Denmark. 2004-2006 Appointed Board Member at the student residence
“Sablons”, Neuchâtel, Switzerland. Row ing and volleyball.
REFERENCES
Prof. Daniel Branton
Harvard Uni.
phone: +1 617 495 2646
e-mail: dbranton@harvard.edu
Prof. Urs Staufer
Technical Uni. Delft, the Netherlands
phone: +31 (0)15-27 86578
e-mail: U.Staufer@tudelft.nl
Prof. Martin Dufva
DTU Nanotech, Technical Uni. Denmark,
phone: +45 4525 5700
e-mail: dufva@mic.dtu.dk
Dr. Hanne Kaas
DHI, Denmark
phone: + 45 4516 9538
e-mail: hka@dhigroup.com
PUBLICATION LIST
International refereed scientific journals:
A.Han, D. Vlassarev, J. Wang, J. A. Golovchenko, D. Branton. 2010 Ice Lithography for Nanodevices. Nanolett.
A. Han, M. Creus, G. Schu rmann, V. Linder, T. R. Ward, N. F. de Rooij, and U. Staufer 2008 Label-Free Detection
of Single Protein Molecules and Protein−Protein Interactions Using Synthetic Nanopores . Anal. Chem., 2008, 80,
4651-4658.
A. Han, G. Schürmann, G. Mondin, R. Bitterli, N. Hegelbach, N. F. de Rooij, and U. Staufer. 2006.
Sensing Protein Molecules Using Nanofabricated Pores. Appl. Phys. Lett., 88, 093901.
A. Han, N.F. de Rooij, and U. Staufer. 2006. Design and fabrication of nanofluidic devices by surface
micromachining. Nanotech., 2006, 17, 2498-2503.
A. Han, G. Mondin, N.G. Hegelbach, N.F. de Rooij, and U. Staufer. 2005. Filling Kinetics of Liquids in
Nanochannels as Narrow as 27nm by Capillary Force. J. Colloid Interface Sci., 293, 151-157.
A. Han, M. Dufva, E. Belleville and C. B. V. Christensen, 2003. Detection of Analyte Binding to Microarrays using
Gold Nanopart icle Labels and a Desktop Scanner. Lab on a Chip, 3, 329-331.
U. Staufer, T. Akiyama, M.R. Gullo, A. Han, R. Imer, N.F. de Rooij, A. Aebi, A. Engel, P.L.T.M. Frederix, M. Stolz,
N. Friederich, D. Wirz. 2007. Micro- and nanosystems for biology and medicine. Microelectronic Eng., 84, 16811684.
Patent applications (industry):
Quantification of Analyte Molecules Using Multiple Reference Molecules and Correlation Functions
A. Han, C. Steinhauer, K. Garde, H. Kaas, Pub. No WO/2008/116471 2008
Precise title cannot be revealed at present due to unresolved intellectual property issues
A. Han, K. Garde, H. Kaas, patent application 2008
Peer reviewed conference proceedings:
A. Han, G. Schürmann, M. Dadras, N.F. de Rooij, U. Staufer. 2006. Sensing Protein Molecules at Different pH
using Nanofabricated Pores. ICN&T 2006 International Conference on Nanoscience and Technology Place, Basel,
Switzerland. Oral Presentation
A. Han, G. Mondin, N. Hegelbach-Guye, N.F. de Rooij, U. Staufer. 2005. Filling Kinetics of Liquids in Nanochannels
down to 27 nm Deep by Capillary Force. First International Nanofluidics Workshop, Boekelo, the Netherlands.
Poster Presentation
A. Han, N.F. de Rooij, U. Staufer. 2004. Fabrication of Nanochannels Systems by Surface Micromachining,
Proceedings of NanoTech, Montreux, Switzerland. Poster Presentation
A. Han, G. Mondin, N.G. Hegelbach, N.F. de Rooij, U. Staufer. 2004. Fabrication of Nanochannels Using
Photolithography and Partial Etching of Sacrificial Layer. Micro Total Analysis Systems, Malmö, Sweden. pp309311. Poster Presentation
A. Han, L. Ceriotti, J. Lichtenberg, N. F. de Rooij, and E. Verpoorte. 2003. DNA fragmentation in a microfabricat ed
microfluidic device. 7th lnternational Conference on Miniaturized Chemical and Biochemical Analysts Systems,
Squaw Valley, California, USA. pp575-578. Poster presentation.
L. Ceriotti, A. Dodge, A. Han, G.-L. Lettieri, J. Lichtenberg, N.F. de Rooij, E. Verpoorte. 2004. Microfluidic
approaches for on-chip DNA sample preparation, Hyphenated Techniques in Chromatography 8 (HTC8), Bruges,
Belgium. 2004. Oral Presentation
G.-L. Lettieri, L. Ceriotti, A. Han, J. Lichtenberg, N. F. de Rooij, E. Verpoorte. 2004. Analyzing nucleic acids in
microfluidic devices, 25th International Symposium on Chromatography, Paris, France. 2004. Oral Presentation
G.-L. Lettieri, A. Han, J. Lichtenberg, N.F. de Rooij, E. Verpoorte. 2004. New approaches for DNA sample
preparation in open microchannels, Pittcon, Chicago, USA. Oral Presentation