Monolith NT.115 Series - NanoTemper Technologies GmbH

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10/2014
Monolith NT.115 Series
Product Information
Monolith Instruments
for MicroScale Thermophoresis
www.nanotemper-technologies.com
10/2014
MicroScale Thermophoresis
A technology by NanoTemper
MicroScale Thermophoresis is an easy, fast and precise way to quantify
biomolecular interactions. It measures the motion of molecules along
microscopic temperature gradients and detects changes in their hydration
shell, charge or size.
▶
Judge and improve sample quality immediately
▶
Work with very small amounts and sensitive samples
▶
Analyze in all buffers and bioliquids (cell lysate, serum)
–immobilization-free
▶
▶
2
and get rid of maintenance downtime
Kd s for all molecular weights from ions to ribosomes
www.nanotemper-technologies.com
10/2014
MicroScale Thermophoresis
A powerful technique
MicroScale Thermophoresis (MST) is a powerful technique for quantifying
is induced by an infrared laser, and the directed movement of molecules is
Monolith NT.115 Series | Product Information
3
10/2014
Discover the Application Range
MicroScale Thermophoresis detects interactions between any kind of
biomolecules thus providing a large application range, from ions and small
Thermophoresis, the movement of molecules in temperature gradients, is
not only dependent on the size, but also on the charge and the hydration
shell of the molecule of interest. Therefore, binding events can be detected
nm
0.
1
nm
7
1
nm
nm
nm
75
Size
20
0
10
00
nm
Since MST is performed free in solution without any surface
immobilization, also bulky or sensitive molecule assemblies such as
liposomes, nanodiscs or membrane proteins can be investigated.
MicroScale Thermophoresis (MST)
Ribosomes
Bacteria
Cell
Nucleic acids
Ions
Nucleosomes
Nucleotides
Viruses
Proteins
Atoms
Fragments
Liposomes
NanoDiscs
Antibodies
AntibodyFragments
Peptides
Small
Molecules
4
www.nanotemper-technologies.com
Da
1
Da
15
0
1
kD
a
a
kD
10
0
10
00
kD
a
MW
10/2014
Monolith NT.115 and NT.115 Pico
The NT.115 Series measures biomolecular interactions via detection of
▶
▶
▶
▶
Broad application range
Cat #
1
2
Instrument
Channel 1
Channel 2
Kd)
Sample
Consumption
(per Kd)
NT.115Pico
Pico –
RED
-
1 pM to mM
1
NT.115
Nano –
BLUE
Nano –
1 nM to mM
2
NT.115
Blue/Red
Nano –
BLUE
Nano –
RED
1 nM to mM
2
NT.115
Nano –
Nano –
RED
1 nM to mM
2
Kd
Kd
Monolith NT.115 Series | Product Information
5
10/2014
Technical Details
Monolith Instruments NT.115Series
NT.115
NT.115Pico
2
1
Samples per run
(RED)
Labeling required
1 nM to mM
1 pM to mM
Yes
Yes
-3
M
-11
-3
M
Range of accessible interactions
Biophysical parameters
Enthalpy, Enzyme
Kinetics
Enthalpy, Enzyme
Kinetics
No
No
Yes
Yes
(serum, cell lysate)
Volume per measurement
Molecular weight range (Da)
1
1
Minutes
Minutes
Immobilization required
No
No
Temperature controlled
22 - 45 °C
22 - 45 °C
Maintenance required
No
No
77
7
7
7
10/2014
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10/2014
10/2014
Detection of picomolar Kd
NT.115Pico.
Material was kindly provided by Dr. Ute Curth, Medical Unitersity Hanover, and Dr. Ahmed Besheer,
Novartis, Basel.
Jerabek-Willemsen, M., André, T., Wanner, R., Roth, H. M., Duhr, S., Baaske, P., and Breitsprecher, D.
10/2014
Customer Statements
Basel, Switzerland
“We have used our Nanotemper NT.115 MicroScale Thermophoresis (MST)
we have been able to make comparisons, results from MST agree well with
anisotropy, SPR). However, we particularly appreciate two distinguishing
molecules binding to large labeled molecules, where binding is tight (nM Kd)
and one or both partners aggregate at micromolar concentrations. Secondly,
titrations, particularly for systems that have changes in their conformational
or oligomeric state upon binding. In several cases, multi-phasic titrations
Prof. Dr. Uffe Holmskov, Institute for Molecular Medicine, University of
Southern Denmark, Odense, Denmark
thus, we appreciate that the interactions are investigted free in solution
and can be studied even in cell lysate. We are using MST for a number of
different interactions, mainly to investigate protein-protein interactions but
and biologics projects, with NanoTemper Technologies’ MicroScale
Thermophoresis has proved a valuable tool for characterising small
molecule-protein and protein-protein interactions, as well as for the study
of protein aggregation concentration determination. There is very good
agreement with other technologies such as Surface Plasmon Resonance
(SPR) and Isothermal Titration Calorimetry (ITC), and we are particularly
10/2014
Selected Publications
1.
2.
3.
Jerabek-Willemsen, M., André, T., Wanner, R., Roth, H. M., Duhr, S., Baaske, P., and Breitsprecher,
Structure
4.
thermophoresis provides insights into mechanism and thermodynamics of ribozyme catalysis. RNA
5.
Phosphoconformational Switch that Reduces Microtubule Association. Current Biology
Taft, M. H., Behrmann, E., Munske-Weidemann, L.-C., Thiel, C., Raunser, S., and Manstein, D.
triggered activities of the RNA-binding protein HuR. Cellular Signalling
hepatitis B virus core protein. Proceedings of the National Academy of Sciences
11. Xiong, X., Coombs, P. J., Martin, S. R., Liu, J., Xiao, H., McCauley, J. W., Locher, K., Walker, P. A.,
12.
1524
13.
Monolith NT.115 Series | Product Information
11
10/2014
Europe / International
[email protected]
USA / Canada
NanoTemper Technologies, Inc.
[email protected]
www.nanotemper-technologies.com
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