CURRICULUM VITAE
Joseph A. Bonanno, OD, PhD, FAAO
1004 E. Wylie St. Bloomington, IN 47401 June 2013
EMPLOYMENT HISTORY
Administrative Positions:
Dean, Indiana University School of Optometry
IU School of Optometry, Executive Assoc. Dean for Academic Affairs & Student Admin.
IU School of Optometry, Assoc. Dean for Research
October 20102007-2010
2005-2007
Academic Appointments:
Indiana University Bloomington, Professor, School of Optometry
Univ. of California, Berkeley, Professor, School of Optometry
Univ. of California, Berkeley, Associate Professor, School of Optometry
Univ. of California, Berkeley, Assistant Professor, School of Optometry
19981997-1998
1993-1997
1989-93
Postdoctoral Appointments:
Univ. of California, Berkeley, Physiology-Anatomy (T. Machen)
Louisiana State Univ, Ophthalmology, New Orleans, (S. Klyce)
1988-89
1987-88
Optometric Appointments:
Wills Eye Hospital, Philadelphia
Contact Lens Service, Director
Pennsylvania College of Optometry, Philadelphia, Primary Care Optometric Resident
1982-83
1981-82
EDUCATION
Univ. of Pennsylvania, Philadelphia
Univ. of California, Berkeley
Univ. of California, Berkeley
Univ. of California, Berkeley
1975
1977
1981
1987
B.A.
M.S.
O.D.
Ph.D.
Biology
Molecular Biology
Optometry
Physiological Optics
HONORS AND AWARDS
B.A., cum laude, Univ. of Pennsylvania
University Graduate Fellowship, UC Berkeley
Low Vision Award, UC Berkeley Optometry Class of ’81
American Optometric Foundation Ezell Fellowship
NIH Post-Doctoral Training Grant (T32) Recipient
NIH, National Eye Institute, NRSA F32 Individual Research Fellowship
Fellow American Academy of Optometry (FAAO)
Garland Clay Award, American Academy of Optometry
Glenn Fry Award, American Academy of Optometry
Appointed Fellow of the Indiana Molecular Biology Institute
IU Trustees Teaching Award
ARVO Fellow (Association for Research in Vision & Opthalmology)
1975
1976
1981
1983-86
1984-86
1987-90
1989
1994
2000
2003
2008
2009
Joseph A. Bonanno National Advisory Eye Council
University of Houston, Cornea & Contact Lens Annual Awardee
2009-2012
2012
RESEARCH
Interests:
1. Long standing interest in the physiology of the corneal endothelium; mechanisms of ion and fluid transport;
maintenance of corneal hydration & transparency.
2. Oxygen tension under contact lenses; human corneal oxygen consumption. Currently, collaborating with a
group at Washington University studying relationship of corneal oxygen consumption with anterior chamber
oxygen tension/oxidative stress with Primary Open Angle Glaucoma.
3. Retinal ganglion cell physiological responses to hypertension, ischemia, and retinal hypoxia. This interest has
developed just in the last few years. Early collaboration with a colleague at IU using a rat glaucoma model.
4. Emerging interest in genetic mutations associated with Fuchs Endothelial Dystropy & CHED
(CONGENITAL HEREDITARY ENDOTHELIAL DYSTROPHY). Currently studying the transport properties
of SLC4A11, a putative borate transporter that is mutated in CHED and late onset Fuchs.
CURRENT SUPPORT
RO1 EY008834-22 (P.I.- J. Bonanno)
4/1/1991- 3/31/2017
27% Effort
NIH-NEI (National Eye Institute)
$250,000 annual direct costs
Regulation of pHi and Fluid Transport of Corneal Endothelium
1K08EY019537-01 (P.I.- T. Nguyen)
NEI/NIH
Lactic acid transport across the corneal endothelium
9/2009-8/2014
0% (Mentor)
$141,964 annual direct costs
No pending applications
PAST SUPPORT
3R01EY008834-18S1 (P.I.- J. Bonanno)
09/30/2009 – 09/29/2011
NIH-NEI (National Eye Institute)
$385,000 total
Regulation of pHi and Fluid Transport of Corneal Endothelium
0% (Supplement)
R24 EY016661 (P.I.- J. Bonanno)
NEI/NIH
Vision Science at Indiana University” (Electronics Core)
9/30/2005-8/31/2010
$71,000 annual direct costs
RO1 EY12934 (P.I.- J. Bonanno)
NEI/NIH
Corneal Metabolic Activity in Humans
3/2001-2/2004
10% Effort
$150,000 annual direct costs
RO1 EY00834-11S1 (P.I.- J. Bonanno)
NEI/NIH
Confocal Microscope
9/2002
RO1 EY00834-3S1 (P.I. J. Bonanno)
NEI/NIH
5/1995
10% Effort
0% Supplement
$400,000
0% (Mentor)
$27,000
2 Joseph A. Bonanno Diversity Student Supplement
RO3EY008347 (P.I.- J. Bonanno)
NEI/NIH
Bicarbonate Transport in Corneal Endothelium
8/1/1989-7/31/1991
$25,000 annual direct costs
Other Support:
IU Bloomington Metacyt, Neuroscience Node, $75,000
In vivo detection of RGC dysfunction
25% Effort
2008-2011
CIBA Vision “Tear Oxygen Tension While Wearing Silicone Hydrogel Lenses”
$240,000 Total Costs
2006-07
American Optometric Foundation – Vistakon Grant Award “Peripheral Tear Oxygen
Tension Under Silicone-Hydrogel Contact Lenses”, $25,000
2005-06
FacultyResearchSupport Program IUB “Fluorescence Based Imaging of Retinal
Ganglion Cell Function” $100,000
2005
SSRF-IUB, School of Optometry Animal Facility Renovation, $500,000
2005
RFF-IUB award, IUB, Ocular Fluorimeter, $60,000
1999
Polymer Technology (Bausch & Lomb) unrestricted research gift $25,000
1998
Vistakon (Johnson & Johnson) unrestricted research gift $50,000
1995
UC Berkeley Committee on Research Pilot Grant $15,000
1993
UC Berkeley Committee on Research Pilot Grant, $15,000
1989
American Optometric Association Research Grant, $4,000
1986
FDA Investigational Drug
IND # 53,160 For use of BSA-Pd Coproporphyrin as a contact lens oxygen sensor
1998-
Consulting
Allergan Pharmaceuticals, Irvine CA
CIBA VISION, Atlanta, GA
Nelson Mullins, LLP Expert Reports
Morrison & Foerster LLP
1995
2005-2006
2008-2009
2012
TEACHING ACTIVITIES
Integrative Optometry, PBL approach, Developer and Instructor-in-Charge
General Physiology, Indiana University Optometry
Medical Biochemistry and Physiology, Indiana University Optometry
3 20062000
1998-
Joseph A. Bonanno Medical Biochemistry and Physiology, UC Berkeley
Ocular Physiology, UC Berkeley
PhD Students
Claude Giasson, OD, PhD
Dan Harvitt, PhD, OD
Qiang Xie, MD, PhD
Yan Zhang, MD, PhD
Dongmei Xing, MD, PhD
Kah Tan Allen , PhD
Tracy Nguyen, OD, PhD
Supryia Jalimarada
Wenlin Zhang, MD
1993-97
1989-1998
1996
1998
2005
2005
2007
2008
2011
2013 expected
current
PROFESSIONAL SERVICE
Member, National Advisory Eye Council (NAEC), NIH
2009-2012
Member, American Academy of Optometry Research Committee
1999-2004; 2011Member, CSR-AED (Anterior Eye Disease, formally VisA) NIH Study Section
2004-2008
NEI Small grants for pilot research reviewer
2003
NEI Training grants reviewer
2007
NEI Small Business grants reviewer
2001
Ad Hoc NIH grant reviewer, VISA study section
1994, 1995, 1997, 2000, 2001
Journal Editorial Board Member:
BMC Physiology
Eye and Contact Lens Journal (formerly CLAO)
Journal Reviewer:
Optom & Vis Sci
Am J Physiology
J Physiology (London)
Invest Ophthalmol & Vis Sci
Exp Eye Res
Am J of Ophthalmol
PNAS (Proceedings of the National Academy of Sciences)
Ophthalmology
Ophthalmic Research
Pharmaceutical Research
Curr Eye Res
Cornea
Archives of Ophthalmology
Molecular Vision
Member, National Board of Examiners in Optometry, Ocular Biology & Disease
Examination Committee
Member, National Board of Examiners in Optometry, Council
4 1999-2008
2006-2007
Joseph A. Bonanno Association for Research in Vision and Ophthalmology (ARVO), Program Planning
Committee, Physiology-Pharmacology,
Chair 2006-07: organized symposium on carbonic anhydrases.
2004-2007
International Society for Eye Research (ISER) annual meeting section organizer.
“Use of Molecular techniques in transport physiology”
2002
MAJOR SERVICE TO SCHOOL
Associate Dean for Academic Affairs & Student Administration Associate Dean for Research
Chair, Curriculum Committee Indiana University Optometry
Major revision completed, Implementation of New Curriculum, IUB Optometry
Developed & Chaired, Faculty Evaluation Committee
Chair, Curriculum Committee, UC Berkeley Optometry
MAJOR SERVICE TO UNIVERSITY
Indiana Clinical & Translational Science Institute Young Investigator Planning Group
Indiana Clinical & Translational Science Institute Grant Reviewer
Member, IU Bloomington Campus Curriculum Committee
Member, IU Bloomington Conflict of Interests Committee,
Member, IU Bloomington Faculty Council,
Member, IU Bloomington Faculty Affairs Committee,
Member, IU Bloomington Tenure Advisory Committee,
Member, UC Berkeley Animal Care and Use Committee
2007-­‐ 2005-2007
2000-2006
20061999-2002
1995-98
2008-2011
2008-2011
2007-2010
2005-2007
2000-2003
1999-2002
1998-2002
1995-98
PROFESSIONAL ASSOCIATION MEMBERSHIPS
Association for Research in Vision and Ophthalmology (ARVO)
FASEB, American Physiological Society
American Association for the Advancement of Science
Fellow of the American Academy of Optometry
Association of Schools and Colleges of Optometry, member of board
American Optometric Association
Indiana Optometric Association
INVITED LECTURES
1.
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8.
Bausch and Lomb Research Symposium on Contact Lenses, August 1985.
Bausch and Lomb Research Symposium on Contact Lenses, August 1987.
Symposium of Corneal Physiology, American Academy of Optometry, 1989
Bausch and Lomb Research Symposium on Contact Lenses, August 1990.
Bethesda Eye Institute, St. Louis University, Dept. of Ophthalmology, Research seminar series, “Corneal
Endothelial Function”, February 1991.
Morton D. Sarver Contact Lens Research Symposium, January 1992.
UC Berkeley Optometry, Therapeutics course: "The Infectious Process", August 1992.
UC Berkeley Optometry, Berkeley Practicum, "AIDS and Optometry", December 1993.
5 9.
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14.
15.
Joseph A. Bonanno Morton D. Sarver Contact Lens Research Symposium, January 1993.
Ocular Fluorescence Symposium, ARVO 1993
Morton D. Sarver Contact Lens Research Symposium, January 1994.
UCBSO, Berkeley Practicum, “Aqueous humor production and outflow”, December 1994
Morton D. Sarver Contact Lens Research Symposium, January 1995.
Southern College of Optometry, “Distinguished Research Lectures”, April 1995.
International Society for Contact Lens Research, Effect of Contact Lenses on corneal physiology,
September 1995.
16. American Academy of Optometry: “Clinical Implications of Reduced Corneal pH” Dec 1995.
17. UC Berkeley School of Optometry, Continuing Ed. in So. Cal., “Aqueous humor production and outflow”,
Feb. 1996.
18. World Congress on the Cornea, endothelium conference, “Bicarbonate transport in corneal endothelium”,
Orlando Fl, May 1996.
19. Oxyopia Lecture, “Ion transport in corneal endothelium”, UC Berkeley Vision Science program, October
1996.
20. Ninth Symposium on the Material Science and Chemistry of Contact Lenses, “Non-invasive measurement
of tear oxygen tension beneath contact lenses” November 1997.
21. Morton D. Sarver Contact Lens Research Symposium, January 1998.
22. Indiana University, Bloomington, Medical Sciences Lecture Series, November 1998: “Maintenance of
Corneal Transparency, Role of bicarbonate transport”.
23. Indiana University, Bloomington, School of Optometry Continuing Education Series: “Roles of
Ultrafiltration and secretion in Aqueous Humor Production”, July 1999.
24. Indiana University, Bloomington, School of Optometry Oxyopia Lecture, November 1998: “Measurement
of tear oxygen tension under contact lenses by a new non-invasive technique”.
25. Indiana University, School of Medicine, Indianapolis, Dept. of Physiology & Biophysics, “Maintenance of
Corneal transparency: Role of the Corneal Endothelium”, Sept. 1999.
26. University of Louisville, Dept. of Physiology, “Bicarbonate transport by the Corneal Endothelium”,
October. 1999.
27. International Society of Ocular Fluorometry, Fort Lauderdale, Fl., April 2000. “Measurement of tear oxygen
tension under contact lenses in human subjects”.
28. International Society for Eye Research, Congress meeting “Role of AQP1 in CO2 Permeability of Corneal
Endothelial Cells”, October 2000.
29. The Glenn Fry Award Lecture, “Explorations of the Cornea”, American Academy of Optometry Annual
meeting, December 2000.
30. Contact Lenses for a new millennium: CLAO symposium. “Etiology of corneal swelling variability”, San
Diego, CA. August 2002.
31. UC Berkeley, School of Optometry, Oxyopia lecture (2/02). “Bicarbonate transport in corneal endothelium”
32. Indiana University, School of Optometry, Oxyopia lecture (9/02): “Can the variability in corneal swelling be
explained by the variability in corneal metabolism?”
33. Indiana University/Purdue University; Graduate course IUPUI-F710; Photoreceptor Biochemistry and
Signal Transduction”, Fall 2002.
34. Ohio State University, School of Optometry, March 2003, “Variability in Hypoxia-Induced Corneal
Swelling and Adaptation in Human Subjects”.
35. CREST (corneal and refractive surgery group, Mass Eye and Ear) Hypoxia adaption in the cornea, May
2003.
6 Joseph A. Bonanno 36. International Society for Contact Lens Research, Sept. 2003, “Corneal Hypoxia: A bad thing could be
good”.
37. Indiana University/Purdue University; Graduate course IUPUI-F710; Photoreceptor Biochemistry and
Signal Transduction”, Fall 2003.
38. IU, School of Optometry, Oxyopia lecture (Fall 2004). “Ion transport in corneal endothelium”
39. Eye and Contact Lens Journal Editorial meeting; “Protection of keratocytes by hypoxia preconditioning”.
(May 2005)
40. Ohio State University, School of Optometry, “Oxygen tension under contact lenses & corneal oxygen
consumption”. (July 2005)
41. University of Tennesse, Memphis; Dept. of Physiology. “New Thoughts on Corneal Endothelial Pump”,
March 2007.
42. Ohio State University, School of Optometry, “Corneal Physiology”. (July 2007)
43. Washington University, St. Louis, Department of Ophthalmology, New Thoughts on Corneal Endothelial
Function”, March 2008.
44. University of Arizona, Dept. of Ophthalmology & Physiology, “Role of HCO3- and Carbonic Anhydrases in
Corneal Endothelial Function” March 2008.
45. UC Berkeley, Vision Science Group, “Role of lactate transport in corneal endothelial function”, March
2010. 46. IUSO Continuing Education, “New Horizons in Ocular Genetic Therapy” July 2010 47. British Contact Lens Association, Keynote Speaker: “The Oxygen Story”
May 2011.
48. IUPUI Biology “Lactate Transport, Osmotic Flux, and Maintenance of Corneal Transparency”, March 2012.
49. Gordon Research Conference, Ventura, CA “The nature of the Corneal Endothelial Pump”, March 2012.
50. Indiana Univ School of Optometry Continuing Education. August 2012 “Why are CAIs contraindicated in
patient with low endothelial cell count?
51. American Academy of Optometry, Primary Care/Anterior Segment Symposium, “Why are CAIs
contraindicated in patient with low endothelial cell count?” Ocotber 2012.
52. Univ. of Houston, College of Optometry, Distinguished Award Lecture in Cornea & Contact Lenses.
December 2012 7 Joseph A. Bonanno PUBLISHED PAPERS
1.
Bonanno JA and Polse KA: Central and peripheral corneal swelling accompanying soft lens extended
wear. Am J Optom Physiol Optics, 62:74-81, 1985.
2.
Bonanno JA, Polse KA and Goldman MN: Effect of soft lens power on peripheral corneal edema. Am J
Optom Physiol Optics, 63:520-526, 1986.
3.
Polse KA, Sarver MD, Kenyon E and Bonanno JA: Gas permeable hard contact lens extended wear:
Ocular and visual responses to a 6-month period of wear. Contact Lens Association of Ophthalomology
13:31-38, 1987.
4.
Bonanno JA and Polse KA: Measurement of in vivo human corneal pH: Open and closed eyes. Invest
Ophthalmol Vis Sci 28:522-530 1987.
5.
Bonanno JA and Polse KA: Corneal acidosis during contact lens wear: Effects of hypoxia and carbon
dioxide. Invest Ophthalmol Vis Sci 28:1514-1520, 1987.
6.
Bonanno JA and Polse KA: Effect of rigid gas-permeable contact lens oxygen transmissibility on stromal
pH in the living human eye. Ophthalmology 94:1305-1309, 1987.
7.
Polse KA, Rivera RK and Bonanno JA: Ocular effects of hard gas-permeable lens extended wear. Am J
Optom Physiol Optics 65:358-364, 1988.
8.
Bonanno JA, Klyce SD and Cragoe EJ: Mechanism of chloride uptake in rabbit corneal epithelium. Am J
Physiol 257:C290-C296., 1989.
9.
Bonanno JA and Machen TE: Intracellular pH regulation in basal corneal epithelial cells measured in
corneal explants: Characterization of Na/H exchange. Exp Eye Res 49:129-142, 1989.
10. Bonanno JA: Lactate-proton cotransport in rabbit corneal epithelium. Curr Eye Res, 9:707-712, 1990.
11. Wolosin JM, Bonanno JA, Hanzel D and Machen TE: Bicarbonate transport mechanisms in rabbit ciliary
body epithelium. Exp Eye Res 52:397-407,1991.
12. Bonanno J.A.: K+/H+ Exchange, A fundamental cell acidifier in corneal epithelium. Am J Physiol, 260
(Cell Physiol. 29): C618-C625, 1991.
13. Bonanno J.A.: Regulation of corneal epithelial pH. Optometry and Vision Sci. 68:682-686, 1991.
14. Cohen S, Polse K, Brand R, and Bonanno JA: Stromal acidosis affects corneal hydration control. Invest
Ophthalmol Vis Sci 33(1): 134-142, 1992.
15. Polse KA, Rivera R, Gan C, Bonanno J, and Cohen S.: Contact lens wear affects corneal pH:
Implications and new directions for contact lens research. J British Contact Lens Assoc, 15:171-177, 1992.
8 Joseph A. Bonanno 16. Bonanno, J.A. and Giasson C.: Intracellular pH regulation in fresh and cultured bovine corneal
endothelium. I. Na+/H+ exchange in the absence and presence of HCO3-. Invest Ophthalmol Vis Sci,
33:3058-3067, 1992.
17. Bonanno, J.A. and Giasson C.: Intracellular pH regulation in fresh and cultured bovine corneal
endothelium. II. Na+:HCO3- cotransport and Cl-/HCO3- exchange. Invest Ophthalmol Vis Sci, 33:30683079, 1992.
18. Rivera R., Gan C, Polse K, Bonanno JA, and Fatt I: Contact lenses affect corneal stromal pH. Optometry
and Vision Science 70(12):991-997, 1993.
19. Giasson, C. and Bonanno, J. A.: Corneal epithelial and aqueous humor acidification during in vivo contact
lens wear in rabbits. Invest. Ophthalmol. Vis. Sci.35(3):851-861, 1994.
20. McNamara, N., Polse, K.A. and Bonanno, J.A.: Stromal acidosis modulates corneal swelling. Invest.
Ophthalmol. Vis. Sci.35(3):846-850, 1994.
21. Bonanno, J. A.: Bicarbonate transport in bovine corneal endothelium under nominally bicarbonate free
conditions. Exp. Eye Res, 58, 417-422, 1994.
22. Giasson, C. and Bonanno, J.A.: Facilitated transport of lactate by rabbit corneal endothelium. Exp Eye
Res, 59: 73-81, July 1994.
23. Giasson, C. and Bonanno, J.A.: Acidification of rabbit corneal endothelium during contact lens wear in
vitro. Curr. Eye Res, 14:311-318, April 1995.
24. Bonanno, JA, Srinivas, SP and Brown, M: Effect of acetazolamide on intracellular pH and bicarbonate
transport in bovine corneal endothelium. Exp Eye Res., 60:425-434, 1995.
25. Cohen, SR, Polse KA, Brand, RJ and Bonanno, JA. The association between pH level and corneal
recovery from induced edema. Curr Eye Res 14:349-355, 1995
26. Bonanno, JA Contact lens induced corneal acidosis. CLAO Journal 22(1):70-74, 1996.
27. Harvitt, DM and Bonanno, JA. Direct non-invasive measurement of tear oxygen tension beneath gas
permeable contact lenses in rabbits. Invest Ophthalmol Vis Sci 37(5): 1026-1036, 1996.
28. Ren H, Petroll WM, Jester JV, Cavanagh HD, Mathers WD, Bonanno JA and Kennedy RH. Adherence of
Pseudomonas aeruginosa to shed rabbit corneal epithelial cells after overnight wear of contact lenses.
CLAO J 23: 63-67, 1997.
29. Srinivas SP and Bonanno, JA. Measurement of Changes in Cell Volume Based on Fluorescence
Quenching. Am J Physiol 272: C1405-C1414, 1997.
30. Bonanno JA and Srinivas SP. Cyclic AMP activates anion conductance in cultured bovine corneal
endothelial cells. Experimental Eye Research 64: 953-962, 1997.
9 Joseph A. Bonanno 31. Sarroca A, Lin S and Bonanno JA. The effects of low pH and CO2 on bovine corneal lactate production.
CLAO Journal 23(4): 1-4, 1997.
32. Harvitt DM and Bonanno JA. Oxygen consumption of the rabbit cornea. Invest Ophthalmol Vis Sci,
39(2): 444-448, 1998.
33. Srinivas SP, Hughes BA and Bonanno JA. Assessment of swelling activated Cl- channels using the halidesensitive fluorescent indicator SPQ. Biophysical Journal, 75: 115-123, 1998
34. Srinivas SP, Yeh JC, Ong A Bonanno JA, Ca2+ mobilization in bovine corneal endothelial cells by P2
purinergic receptors. Curr Eye Res. 17:994-1004, 1998.
35. Harvitt DM and Bonanno, JA. pH dependence of corneal oxygen consumption. Invest Ophthalmol Vis
Sci., 39: 2778-81, 1998.
36. McNamara NA, Polse KA and Bonanno JA. Fluorophotometry in contact lens research: The Next Step.
Optometry and Vision Science, 75:316-22, 1998.
37. Bonanno JA, Yi G, Kang XJ and Srinivas SP. Re-evaluation of Cl-/HCO3- exchange in cultured bovine
corneal endothelial cells. Invest Ophthalmol Vis Sci., 39: 2713-2722, 1998.
38. Srinivas SP, Yi Guan and Bonanno JA. Swelling activated chloride channels in bovine corneal endothelial
cells. Exp Eye Res. 68:165-177, 1999.
39. Bonanno JA, Yi Guan, S. Jelamskii, and X. J. Kang. Apical and basolateral CO2- HCO3-permeability in
cultured bovine corneal endothelial cells. Am J Physiol: Cell 277: C545, 1999.
40. Harvitt D and Bonanno, JA. Re-evaluation of the oxygen diffusion model for predicting minimum contact
lens Dk/L values needed to avoid corneal anoxia. Optometry and Vision Science 76:712-719, 1999.
41. Jelamskii S, Sun X.C., Herse P., and Bonanno JA. Baso-lateral Na+:K+:2Cl- cotransport in cultured and
fresh bovine corneal endothelium. Invest Ophthalmol Vis Sci. 41:488-495, 2000.
42. Sun X.C., Bonanno J.A., Jelamskii S. and Xie Q. Expression and localization of the Na/HCO3
cotransporter (NBC) in bovine corneal endothelium. Am. J. Physiol. Cell Physiol., 279:C1648-C1655,
2000.
43. Sun X.C., Allen K.T., Xie Q., Stamer W.D. and Bonanno J.A. Effect of AQP1 expression level on CO2
permeability in bovine corneal endothelium. Invest Ophthalmol Vis Sci.42:417-423, 2001.
44. Sun X.C., McCutheon C., Bertram P., Xie Q. and Bonanno J.A. Studies on the expression of anion
transport related proteins in corneal endothelial cells. Cur. Eye. Res., 22:1-7, 2001.
45. Bonanno, J.A. Effects of contact lens-induced hypoxia on the physiology of the corneal endothelium.
Optom Vis Sci. 78:783-790, 2001.
10 Joseph A. Bonanno 46. Sun X.C. and Bonanno, J.A. Expression, Localization and Functional Evaluation of CFTR in Bovine
Corneal Endothelial Cells. Am J Physiol Cell Physiol 282(4):C673-83, 2002.
47. Joseph A. Bonanno, Thomas Stickel, Tracy Nguyen, Trina Biehl, Donna Carter, William J. Benjamin, P.
Sarita Soni. “Direct Estimation of Human Corneal Oxygen Consumption by Non-invasive Measurement of
Tear Oxygen Tension While Wearing Hydrogel Contact Lenses”. Invest. Ophthalmol. Vis. Sci. 43:371376, 2002.
48. Zhang, Y, Xie, Q, Sun, X.C. and Bonanno, J.A. Enhancement of HCO3- permeability across the apical
membrane of bovine corneal endothelium by multiple signaling pathways. Invest. Ophthalmol. Vis. Sci.
43:1145-53, 2002.
49. Xie, Q., Zhang, Y., Zhai, CB. and Bonanno, J.A. Calcium Influx Factor from Cytochrome P-450
Metabolism and Secretion-like Coupling Mechanisms for Capacitative Calcium Entry in Corneal
Endothelial Cells. J. Biol. Chem. 277(19):16559-66, 2002.
50. Srinivas SP, Ong A, Goon L, Goon L, Bonanno J. A. Lysosomal Ca(2+) stores in bovine corneal
endothelium. Invest Ophthalmol Vis Sci. 2002 Jul;43(7):2341-50.
51. Srinivas SP, Ong A, Zhai CB and Bonanno JA. Inhibition of carbonic anhydrase activity in cultured
bovine corneal endothelial cells by dorzolamide. Invest Ophthalmol Vis Sci 43: 3273-3278, 2002.
52. Stickel TE and Bonanno JA. The relationship between corneal oxygen tension and hypoxic corneal
edema. Optometry. 73: 598-604, 2002.
53. Bonanno, J.A., “Identity and Regulation of Ion Transport Mechanisms in the Corneal Endothelium”,
Progress in Retina and Eye Research, 22: 69-94, 2003
54. Xing Cai Sun, Chang-Bin Zhai, Miao Cui, Yanqiu Chen, Lonny R. Levin, Jochen Buck, Joseph A.
Bonanno. HCO3--Dependent Soluble Adenylyl Cyclase Activates the Cystic Fibrosis Transmembrane
Conductance Regulator in Corneal Endothelium. Am. J. Physiol. (Cell Physiology), 284: C1114-C1122,
2003.
55. Joseph A. Bonanno, Tracy Nguyen, Trina Biehl, Sarita Soni. "Can variability in corneal metabolism
explain the variability in corneal swelling?" Eye and Contact Lens Research 29(1S): S7-9, 2003.
56. Tracy Nguyen, P. Sarita Soni, Edward Brizendine, Joseph A. Bonanno. “Variability in hypoxia-induced
corneal swelling is associated with variability in corneal metabolism and endothelial function” Eye and
Contact Lens, 29: 117-125, 2003.
57. Soni PS, Nguyen TT, Bonanno JA. Overnight orthokeratology: visual and corneal changes.
Eye and Contact Lens. 2003 Jul; 29(3):137-45.
58. Sun XC, Bonanno JA. Identification and cloning of the Na/HCO(3-) cotransporter (NBC) in human corneal
endothelium. Exp Eye Res. 2003 Sep; 77(3):287-95.
11 Joseph A. Bonanno 59. Srinivas SP, Bonanno JA, Lariviere E, Jans D, Van Driessche W. Measurement of rapid changes in cell
volume by forward light scattering. Pflugers Arch. Physiol. 2003 Oct; 447(1):97-108.
60. Sun, XC, Cui, M, and Bonanno, JA. [HCO3-]-regulated expression and activity of soluble adenylyl cyclase
in corneal endothelial and Calu-3 cells. BMC Physiol. 2004 Apr 29;4(1):8.
61. Soni PS., Nguyen TT., Bonanno, JA. Overnight Orthokeratology. Refractive and corneal recovery after
discontinuation of reverse-geometry lenses. Eye & Contact Lens 2004 Oct 30:254-262.
62. Jinhua Li, Xing Cai Sun, and Joseph A Bonanno. Role of NBC-1 in Apical and Basolateral HCO3Permeabilities and Transendothelial HCO3- Fluxes in Bovine Corneal Endothelium. Am J Physiol Cell
Physiol. 2005 Mar; 288(3):C739-46.
63. Kah Y. Tan-Allen, Xing Cai Sun and Joseph A. Bonanno. Characterization of Adenosine Receptors in
Bovine Corneal Endothelium. Exp Eye Res. 2005 May;80(5):687-96.
64. Qiang Xie, Yan Zhang, Xing Cai Sun, Changbin Zhai, Joseph A. Bonanno. Expression and Functional
Evaluation of Transient Receptor Potential Channel 4 in Bovine Corneal Endothelial Cells. Exp Eye Res.
2005 Jul;81(1):5-14.
65. Xie Q, Lin T, Zhang Y, Zheng J, Bonanno JA. Molecular cloning and characterization of a human AIF-like
gene with ability to induce apoptosis. J Biol Chem. 2005 May 20, 280:19673-19681.
66. Hua Yang, Stefan Mergler, Xingcai Sun, Zheng Wang, Luo Lu, Joseph A. Bonanno, Uwe Pleyer, and Peter
S. Reinach. TRPC4 Knockdown Suppresses Epidermal Growth Factor-induced Store-operated Channel
Activation and Growth in Human Corneal Epithelial Cells J. Biol. Chem. 2005; 280 32230-32237.
67. Dongmei Xing, Xingcai Sun, Jinhua Li, Miao Cui, Kah Tan-Allen and Joseph A. Bonanno; Hypoxia
preconditioning protects corneal stromal cells against induced apoptosis. Exp Eye Res 2006; 82:780.
68. Yan Zhang, Jinhua Li, Qiang Xie and Joseph A. Bonanno. Molecular expression and functional
involvement of the bovine calcium-activated chloride channel 1 (bCLCA1) in apical HCO3− permeability of
bovine corneal endothelium Exp Eye Res. 2006 Nov; 83(5):1215-24.
69. Cheng Q, Nguyen T, Song H, Bonanno J. Hypoxia protects human corneal endothelium from tertiary butyl
hydroperoxide and paraquat-induced cell death in vitro. Exp Biol Med (Maywood). 2007 Mar; 232(3):44553.
70. Xing Cai Sun, Jinhua Li, Miao Cui, and Joseph A. Bonanno. Role of Carbonic Anhydrase IV in Corneal
Endothelial HCO3- Transport. Invest. Ophthalmol. Vis Sci. 2008, 49: 1048-1055.
71. Jinhua Li, KT Allen, X Sun, and JA Bonanno. Dependence of cAMP meditated increases in Cl(-) and
HCO(3)(-) permeability on CFTR in bovine corneal endothelial cells. Exp Eye Res. 2008 Apr; 86(4): 68490.
12 Joseph A. Bonanno 72. Xing D, Bonanno JA. Effect of cAMP on TGFbeta1-induced corneal keratocyte-myofibroblast
transformation. Invest Ophthalmol Vis Sci. 2009; 50:626–633.
73. Hypoxia preconditioning protection of corneal stromal cells requires HIF1alpha but not VEGF. Xing D,
Bonanno JA. Mol Vis. 2009 May 18; 15:1020-7.
74. Liu H, Begley CG, Chen M, Bradley A, Bonanno JA, McNamara N, Nelson JD, Simpson T. A link
between tear instability and hyperosmolarity in dry eye. Invest Ophthalmol Vis Sci. 2009 50: 3671-3679.
75. Tear oxygen under hydrogel and silicone hydrogel contact lenses in humans. Bonanno JA, Clark C, Pruitt
J, Alvord L. Optom Vis Sci. 2009 Aug;86(8):E936-42.
76. Hypoxia reduces TGFbeta1 induced corneal keratocyte myofibroblast transformation. Dongmei Xing and
Joseph A. Bonanno. Molecular Vision 2009; 15:1827-1834.
77. Lan Huang, Matt Harkenrider, Meredith Thompson, Pingyu Zeng, David Gilley, David A. Ingram, Joseph
A. Bonanno, and Mervin C. Yoder. A hierarchy of endothelial colony forming cell activity is displayed by
bovine corneal endothelial cells. Invest Ophthalmol Vis Sci. 2010 Aug; 51(8):3943-9.
78. Cailing Liu, Qiang Cheng, Tracy Nguyen, Joseph A. Bonanno. Knockdown of NBCe1 in vivo
compromises the corneal endothelial pump. Liu C, Cheng Q, Nguyen T, Bonanno JA. Invest Ophthalmol
Vis Sci. 2010 Oct; 51(10):5190-7 May 5.
79. Li S, Allen KT, Bonanno JA. Soluble adenylyl cyclase mediates bicarbonate -dependent corneal
endothelial cell protection. Am J Physiol Cell Physiol. 2011 Feb; 300(2):C368-74
80. Joseph A. Bonanno. “Molecular Mechanisms Underlying the Corneal Endothelial Pump”. Experimental
Eye Research, 2012 95:2-7.
81. Oxygen-Deficient Metabolism and Corneal Edema. Leung BK, Bonanno JA, and Radke C. Progress in
Retinal and Eye Research, 2011 Nov;30(6):471-92
82. Tracy T. Nguyen, Joseph A. Bonanno. Bicarbonate and Carbonic Anhydrase Activity Facilitate Lactate-H+
Transport Across Bovine Corneal Endothelium. Invest Ophthalmol Vis Sci. 2011 Oct 17;52(11):8086-93.
83. Liu C, Ogando D, Bonanno JA. SOD2 contributes to anti-oxidative capacity in rabbit corneal endothelial
cells. Mol Vis. 2011;17:2473-81.
84. Tracy T. Nguyen, Joseph A. Bonanno. Lactate-H+ transport is a significant component of the in vivo
corneal endothelial pump. Invest Ophthalmol Vis Sci. 2012; 53(4): 2020-9.
85. Liu J, Seet LF, Koh LW, Venkatraman A, Venkataraman D, Mohan RR, Praetorius J, Bonanno JA, Aung
T, Vithana EN. Depletion of SLC4A11 causes cell death by apoptosis in an immortalized human corneal
endothelial cell line. Invest Ophthalmol Vis Sci. 2012; 53(7):3270-9.
86. Thore Schmedt, Yuming Chen, Tracy T. Nguyen, Shimin Li, Joseph A. Bonanno, Ula V. Jurkunas.
13 Joseph A. Bonanno Telomerase immortalization of human corneal endothelial cells yields functional hexagonal monolayers.
PLoS One 2012; 7(12):e51427.
87. Supriya S. Jalimarada, Diego G. Ogando, Eranga N. Vithana, Joseph A. Bonanno. Ion Transport Function
of SLC4A11 in Corneal Endothelium . Invest Ophthalmol Vis Sci. 2013 Jun 6. doi:pii: iovs.13-11929v1.
10.1167/iovs.13-11929. [Epub ahead of print]
88. Diego Ogando, S. Jalamarada, Bonanno, JA. SLC4A11 is an EIPA-sensitive pHi Regulator with Na+ and
NH4+ Permeability. Submitted to AJP Cell.
89. Shimin Li, Tracy Nguyen, Michelle Brown and Bonanno, JA. CD147 Expression Required for Lactate
transporters MCT1 and MCT4 in Rabbit Corneal Endothelium. In Preparation.
BOOK CHAPTERS
1.
Klyce SD and Bonanno JA: The role of the epithelium in corneal hydration. The Cornea: Transactions of
the World Congress on the Cornea III, ed D. Cavanagh, Raven Press, New York, 1988.
2.
Mandell RB, Polse KA and Bonanno JA: Reassessment of optical pachometry. The Cornea: Transactions
of the World Congress on the Cornea III, ed D. Cavanagh, Raven Press, New York, 1988.
3.
Bonanno, JA and Polse KA.: Hypoxic changes in the corneal epithelium and stroma. In: Complications of
contact lens wear. Chap. 2, pp 21-36, ed., A. Tomlinson, Mosby, 1992.
4.
O'Neal M and Bonanno JA.: Effects of contact lenses on corneal physiology. In: Contact Lens Practice,
M. Reuben and M. Guillon eds., Chapman and Hall, London, p969-990, 1994.
5.
Bonanno, J.A. Corneal Edema. In: Anterior segment complications of contact lens wear. ed. J.A. Silbert,
Chap 2, p 15-28, Churchill Livingstone, New York, First 1994; Second Edition, 2000.
6.
Bonanno, J.A. and Srinivas, S.P. “Corneal endothelial signaling mechanisms”. Encyclopedia of the Eye,
Chapter 75. J. Besharse and R. Dana, Eds, Elsevier, 2010, (ISBN 13: 978-0-12-374198-1).
7.
Bonanno, J. A., Miller WL, Bergmanson, J. Corneal Physiology, Chapter 13. In: Clinical Ocular Anatomy
and Physiology, Jan P.G. Bergmanson; 19th edition, 2012
ABSTRACTS
1. Bonanno, J.A. and Polse K.A. Observations on peripheral corneal swelling. Invest Ophthalmol Vis Sci
(suppl) 26:144, 1985.
14 Joseph A. Bonanno 2. Mandell, R., Polse K., Bonanno, J., Vastine D., Demartini D., and Brand R. Corneal Endothelial Function
Testing, Invest Ophthalmol Vis Sci (suppl) 27:174, 1986.
3. Bonanno, J., Polse K., In vivo measurement of human corneal stromal pH. Invest Ophthalmol Vis Sci
(suppl) 27: 66, 1986.
4. Bonanno, J. Polse, K., Effect of contact lens transmissibility on stromal pH. Invest Ophthalmol Vis Sci
(suppl) 28:163, 1987.
5. Polse K., Mandell, R., Vastine, D., Demartini, D., Brand, R., Bonanno, J. and Flom, R. Corneal hydration
dynamics in Fuch's Dystrophy. Invest Ophthalmol Vis Sci (suppl) 28: 171, 1987.
6. Bonanno, J. and Klyce S.D., Effects of furosemide anf low [Na] or [Cl] on stimulated Cl transport in rabbit
corneal epithelium. Invest Ophthalmol Vis Sci (suppl) 29:174, 1988.
7. Klyce, S.D., Dingeldein S., Bonanno, J., McDonald, M., and Kaufman, H. Hydrogel implants: evaluation
of first human trial. Invest Ophthalmol Vis Sci (suppl) 29:393, 1988.
8. Bonanno, J.A. and Machen, T.E. Lactate-proton cotransport regulates pHi during hypoxia in rabbit corneal
epithelial cells. Invest Ophthalmol Vis Sci (suppl) 30: 168, 1989.
9. Wolosin, J.M., Bonanno, J.A. and Machen, T.E. Na dependent HCO3 transport and Cl/HCO3 exchange in
ciliary epithelium. Invest Ophthalmol Vis Sci (suppl) 30:169, 1989.
10. Bonanno, J.A. K dependent H flux in rabbit basal corneal epithelial cells, a K/H exchanger? Invest
Ophthalmol Vis Sci (suppl) 31:2163, 1990.
11.Giasson, C. and Bonanno, J. Effect of PMMA contact lenses on corneal epithelial pHi and aqueous humor
pH in Rabbits. Invest Ophthalmol Vis Sci (suppl) 32:324, 1991.
12. Cohen, S.R., Polse K.A., Janic, G., Brand, R., and Bonanno, J. Stromal acidosis reduces corneal hydration
control. Invest Ophthalmol Vis Sci (suppl) 32:325, 1991.
13. Bonanno, J. and Giasson, C. Bicarbonate transport and pHi regulation in fresh and cultured bovine corneal
endothelium. Invest Ophthalmol Vis Sci (suppl) 32: 2015, 1991.
14. Rivera, RK, Gan CM, Polse KA and Bonanno, JA. Contact lenses affect corneal stromal pH. Invest
Ophthalmol Vis Sci (suppl) 33:24, 1992.
15. Giasson C. and Bonanno J.A. Effect of contact lens transmissibility and carbonic anhydrase inhibition on
corneal epithelial pHi in living rabbits. Invest Ophthalmol Vis Sci (suppl) 33:2992, 1992.
16. Polse, KA, Brand, R, Cohen S and Bonanno, JA. Stromal pH effects on corneal function. Invest
Ophthalmol Vis Sci (suppl) 33: 2993, 1992.
15 Joseph A. Bonanno 17. Bonanno, J.A. Bicarbonate transport in bovine corneal endothelial cells under nominally bicarbonate free
conditions. Invest Ophthalmol Vis Sci (suppl) 33: 3180, 1992.
18. Bonanno, J.A.: Role of carbonic anhydrase in pHi regulation in bovine corneal endothelium. Invest
Ophthalmol Vis Sci (supp) 34:1404, 1993.
19. Giasson, C.J. and Bonanno, J.A. Lactate transport across corneal endotheliumin rabbits. Invest
Ophthalmol Vis Sci (suppl) 34:1403, 1993.
20. Harvitt D.M. and Bonanno, J.A.: Non-invasive measurement of tear oxygen in rabbits. Invest Ophthalmol
Vis Sci (suppl) 34: 1369, 1993.
21. McNamara, N.A., Polse K.A, and Bonanno, J.A.: Effects of pH on corneal swelling. Invest Ophthalmol
Vis Sci (suppl) 34: 2420, 1993.
22. Srinivas, S.P. and Bonanno, J.A.: Measurement of intracellular [Cl] in bovine corneal endothelial cells.
Invest Ophthalmol Vis Sci (suppl) 35:914, 1994.
23. Harvitt, D.M. and Bonanno, J.A.: Non-invasive measurement of tear oxygen tension beneath gaspermeable contact lenses. Invest Ophthalmol Vis Sci (suppl) 35:4200, 1994.
24. Giasson, C.J. and Bonanno, J.A.: Contact lens induced acidosis of rabbit corneal endothelium in vitro.
Invest Ophthalmol Vis Sci (suppl) 35:4201, 1994.
25. Srinivas, SP and Bonanno, JA: Measurement of changes in cell volume of the cultured corneal
endothelium. Invest Ophthalmol Vis Sci 36: abstr #32, 1995.
26. Lin, S.T. and Bonanno, J.A.: Effect of CO2 on rate of lactate production by corena in vitro. Invest
Ophthalmol Vis Sci 36: abstr #689, 1995.
27. Harvitt, D.M. and Bonanno, J.A.: Oxygen tension beneath contact lenses used to asses the effect of topical
ansesthetics on corneal metabolism. Invest Ophthalmol Vis Sci 36: abstr #1446, 1995.
28. Giasson, C, Bonanno, J.A. and Fatt, I.: CO2 tension in the rabbit anterior segment during simulated eye
closure. Invest Ophthalmol Vis Sci 36: abstr #1447, 1995.
29. Sarroca A, Lin S and Bonanno JA. The effects of low pH on corneal lactate production in vitro.
Optometry and Vision Science 72(12s): 229.
30. Harvitt, D.M. and Bonanno, J.A.: Oxygen consumption by the rabbit cornea. Invest Ophthalmol Vis Sci
37: abstr #340, 1996.
31. Bonanno, JA and Srinivas SP.: Stimulation of chloride transport by cAMP in cultured corneal endothelium.
Invest Ophthalmol Vis Sci 37: abstr #3081, 1996.
16 Joseph A. Bonanno 32. Srinivas SP and Bonanno JA: Swelling activated chloride channels in corneal endothelium. Invest
Ophthalmol Vis Sci 37: abstr #3082, 1996.
33. Guan Yi, S.P Srinivas and Bonanno, JA. Estimation of enhancement in Cl permeability upon swelling in
corneal endothelial cells. Invest Ophthalmol Vis Sci 38(4), #4857, 1997.
34. Kuo, C, Srinivas, SP, and Bonanno, JA. Extracellular ATP potentiates RVD in corneal endothelial cells.
Invest Ophthalmol Vis Sci. #4856, 1997.
35. Ong, A, Srinivas, SP, and Bonanno, JA. Activation of Na/H exchange in corneal endothelial cells during
RVI. Invest Ophthalmol Vis Sci. 38(4), #4855, 1997.
36. Bonanno, JA. Polarity of ion transporters in cultured bovine corneal endothelial cells. Invest Ophthalmol
Vis Sci. 38(4), #4854, 1997.
37. Harvitt D, and Bonanno, JA. Acidosis stimulates corneal oxygen consumption in the rabbit. Invest
Ophthalmol Vis Sci. 38(4), #881, 1997.
38. Srinvas SP, Ong A, Bonanno JA. Lysosomal Ca2+ store in cultured corneal endothelial cells. Invest
Ophthalmol Vis Sci. 39(4), #3672, 1998.
39. Yi G, Bonanno JA. Re-examination of anion exchange incultured bovine endothelial cells. Invest
Ophthalmol Vis Sci. 39(4), #3675, 1998.
40. Bonanno JA, Yi G. Apical vs. baso-lateral HCO3- permeability in cultured bovine corneal endothelium.
Invest Ophthalmol Vis Sci. 39(4), #4172, 1998
41. Ong A, Srinivas SP, Bonanno JA. Influence of dorzolamide (TRUSOPT) on CO2/HCO3- transport in
cultured corneal endothelial cells. Invest Ophthalmol Vis Sci. 38(4), #4283, 1998.
42. Harvitt D, and Bonanno, JA. Minimum contact lens transmissibility values for daily and extended wear
contact lenses. Optom Vis Sci. 75(12s), p189, Dec 1998.
43. Bonanno JA, Jelamski S, Sun X. Na:K:2Cl cotransport is expressed in fresh corneal endothelium. Invest
Ophthalmol Vis Sci 40: S91, 1999.
44. Ishimoto B, Grahan A, Lin M, Tieu T, Polse K, Bonanno JA. A new fluorotron for the measurement of
corneal pH. Invest Ophthalmol Vis Sci 40: S909, 1999.
45. Sun X.C., Bonanno J.A. and Xie Q. Expression and localization of NBC in corneal endothelium. Invest
Ophthalmol Vis Sci 41: S560, 2000.
46. Bonanno J.A., Sun X.C., McCutheon C., Xie Q. Preliminary studies on expression of anion transport
related proteins in corneal endothelium and epithelium. Invest Ophthalmol Vis Sci 41: S2778, 2000.
17 Joseph A. Bonanno 47. Srinivas S.P., Goon L.H., Loo A., Goon L. and Bonanno J.A. cAMP-induced changes in cell volume of
corneal endothelial cells. Invest Ophthalmol Vis Sci 41: S2790, 2000.
48. Loo A., Srinivas S.P., Goon L., Goon L.H. and Bonanno J.A. Lysosomal Ca2+ stores in corneal
endothelium are independent of IP3-sensitive stores. Invest Ophthalmol Vis Sci 41: S2793, 2000.
49. Stickel T. and Bonanno J.A. The relationship between corneal oxygen demand and hypoxic corneal
edema. Optometry and Vision Science 77 (12s), 172.
50. Cui M, Srinivas SP, Mutharasan R, Sun X, Bonanno JA, Yue, B. Mechanotransduction in cultured
trabecular meshwork cells. Invest Opthalmol Vis Sci 42:#743, 2001.
51. Xie Q, Zhang Y, Sun X, Bonanno JA. Preliminary study on capacitive calcium entry in corneal endothelial
cells. Invest Opthalmol Vis Sci 42:#1487, 2001.
52. Sun X, Allen KT, Bonanno JA. Cloning and identification of Na/HCO3 cotransporter (NBC-1) in human
corneal endothelium. Invest Opthalmol Vis Sci 42:#2691, 2001.
53. Zhang Y, Xie Q, Bonanno JA. Enhancement of HCO3- flux across the apical membrane of the corneal
endothelium by cAMP and Ca2+-signaling pathways. Invest Opthalmol Vis Sci 42:#2695, 2001.
54. Bonanno JA, Ngyuen T, Biehl T, Carter D, Soni S. Can variability in corneal metabolic activity explain
intersubject variability in hypoxic corneal swelling. Invest Opthalmol Vis Sci 42:#3166, 2001.
55. Srinivas SP, Mutharasan R, Sun X, Bonanno JA. Strectch-activated ATP release by corneal endothelium.
Invest Opthalmol Vis Sci 42:#3579, 2001
56. Yang H, Sun X, Zhai C, Cui M, Bonanno J, Wang Z, and Reinach P. EGF stimulaters growth through
calcium-induced calcium release activation in rabbit corneal epithelial cells. Invest. Ophthalmol. Vis. Sci.
#1652, 2002.
57. Sun X, Cui M, Zhai C, and Bonanno JA. Apical Chloride permeability in corneal endothelium is increased
by activating soluble adenylyl cyclase. Invest. Ophthalmol. Vis. Sci. #3189, 2002.
58. Sun X, Cui M, and Bonanno J. Bicarbonate as an enhancer of soluble adenylyl cyclase activity and
expression in bovine corneal endothelium. Experimental Biology 2002.
59. Zhai C, Sun X, Xie Q, and Bonanno J. Expression of GABA receptors in corneal endothelium.
Experimental Biology 2002.
60. Xie Q, Zhang Y, Zhai C, and Bonanno J. Calcium influx factors from P450 metabolism predominate the
mechanisms for capacitative calcium entry in corneal endothelial cells. Experimental Biology 2002.
61. Zhang Y, Xie Q, and Bonanno J. Enhancement of HCO3- flux across the apical membrane of bovine corneal
endothelium by Ca2+ signaling pathways. Experimental Biology 2002.
18 Joseph A. Bonanno 62. Bonanno, JA, Nguyen T, Biehl T, Coe C, Soni PS. Metabolic adaptation of corneal epithelium following
hypoxia. Invest. Ophthalmol. Vis. Sci. #1369, 2003.
63. Zhang Y, Xie Q, Bonanno JA. Capacitative calcium entry enhances apical HCO3- permeability in bovine
corneal endothelium. Invest. Ophthalmol. Vis. Sci. #3448, 2003.
64. Sun XC, Bonanno JA. The use of a novel membrane-associated pHluorins for measurements of
extracellular surface pH in bovine corneal endothelial cells. Invest. Ophthalmol. Vis. Sci. #4714, 2003.
65. Allen KY, Sun XC, BonannoJA. P1 purinergic receptors in bovine corneal endothelial cells. Invest.
Ophthalmol. Vis. Sci. #4724, 2003.
66. Zhang Y, Xie Q, and Bonanno J. Molecular characterization of calcium activated chloride channels
(bCLCA1) expressed in corneal endothelium. ARVO # 426, 2004.
67. X. SUN and J.A. Bonanno. Evaluating the expression and function of carbonic anhydrase IV in bovine
corneal endothelium. ARVO, # 427, 2004.
68. Xie Q, Zhang Y, and Bonanno J. Immunodetection of transient recptor channel 4 in bovine corneal
endotheliual cells. ARVO, #428, 2004.
69. H. Yang, x, Sun, Q Xie, M Cui, Z Wang, Q Wen, F Zhang, J.A. Bonanno, and P.S. Reinach. Knockdown
of TRPC4 Expression in human corneal epithelial cells suppresses proliferation. ARVO, #430, 2004.
70. J.A. Bonanno and J. Li. siRNA knockdown of 1Na+/2HCO3- cotransport in corneal endothelium. ARVO
#1089, 2004.
71. D. Xing, X. Sun, M Cui, and J.A. Bonanno. Protection of corneal keratocytes by hypoxia preconditioning
protection. ARVO #3845, 2004.
72. Hypoxia Preconditioning Protects Corneal Keratocytes from UV and FAS mediated Apoptosis
J. A. Bonanno, D. Xing, J. Li, M. Cui ARVO #4776, 2005.
73. The Effect of CFTR-siRNA and Vitamin C on Cl- and HCO3- Permeability in Bovine Corneal Endothelium
Cells J. Li and J. A. Bonanno ARVO #2202, 2005
74. P2Y Agonists Induce MLC Dephosphorylation in Corneal Endothelial Cells Through Elevated cAMPK.Y.
Tan-Allen, M. Satpathy, J. Bonanno, S.P. Srinivas, ARVO #2203, 2005.
75.
Hif-1a is responsible for protection of corneal fibroblasts during hypoxia preconditioning. D. Xing J. A.
Bonanno, ARVO #1607. 2006
76. Hypoxia Protects Human Corneal Endothelium from Tertiary Butyl Hydroperoxide and Paraquat-induced
Cell Death in vitro” Q. Cheng and J. A. Bonanno ARVO #4923.2006
77.
Protection of cultured bovine corneal endothelium from staurosporine induced death by adenosine
preconditioning. K.Y. Tan-Allen, J. Bonanno, ARVO #1613.2006.
19 Joseph A. Bonanno 78. Soluble Adenylate Cyclase ( sAC ) Protects Corneal Endothelium from Staurosporine - Induced Apoptosis.
K.Tan-Allen, J.A. Bonanno. #548, ARVO, 2007.
79. Role of Carbonic Anhydrases in Corneal Endothelial Function . JA Bonanno. #1128, ARVO, 2007.
80.
The Effect of Hypoxia and Elevated cAMP on Corneal Keratocyte Myofibroblast Transformation. D.
Xing and JA Bonanno, #1491, ARVO, 2007.
81. In vivo Lentiviral Mediated Gene Delivery to Rabbit Corneal Endothelium. J.A. Bonanno, T.Nguyen,
C.Cheng, H.Song. # 2701, ARVO, 2007.
83. Lactic Acid Transport Across Bovine Corneal Endothelium. T.T. Nguyen, J.A. Bonanno. #2826, ARVO,
2007.
84. A link between tear instability and hyperosmolarity in dry eye. CG Begley, H Liu, M Chen, M Jansen, A
Bradley, and JA Bonanno. #1543, ARVO, 2008.
85. On-­‐eye contact lens central vs. temporal PO2. CF Morgan, G Zhang, L Alvord, JA Bonanno. #2018, ARVO, 2008. 86. In vivo knockdown of sodium-­‐bicarbonate cotransporter NBCe1 in rabbit corneal endothelium by lentiviral gene delivery. C Liu, T Nguyen, Q Cheng, M Calvin, JA Bonanno. #3173, ARVO 2008. 87. Hypoxia and elevated camp reduce TGFbeta1 induced keratocyte myofibroblast transformation through the RhoA pathway. D Xing and JA Bonanno. #4038, ARVO, 2008. 88. Lactate transport in cultured bovine corneal endothelium. TT Nguyen, M Cui, and JA Bonanno. #3936, ARVO, 2008. 89. Protection of corneal endothelium by HCO3-­‐ is through camp signaling generated by soluble adenylate cyclase (sAC). K Tan-­‐Allen and JA Bonanno. #3957, ARVO, 2008. 90. Tear Oxygen tension under silicone-­‐hydrogel lenses in human subjects. JA Bonanno, C Clark, J Pruitt, L Alvord. #4846, ARVO, 2008. 91. Cloning and Characterization of the Borate Transporter SLC4A11in Bovine Corneal Endothelial Cells. K.K.
Dutta, S.J. Shivakumar, T. Nguyen, C. Liu, E. Vithana, J.A. Bonanno. # 1797 ARVO, 2009.
92. Expression of the Borate Transporter NaBC1 (SLC4A11) in Bovine Corneal Endothelial Cells (BCEC). S.S.
Jalimarada, K.K. Dutta, E.N. Vithana2 J.A. Bonanno. #1801 ARVO 2009.
93. Lactic Acid Transport in the Rabbit Corneal Endothelium. T.T. Nguyen, J.A. Bonanno. #1814 ARVO
2009.
94.
SOD2 Gene Expression and SiRNA Knockdown in Rabbit Corneal Endothelial Cells. C. Liu, M. Calvin,
K. Dutta, J. Bonanno. #1825 ARVO 2009.
20 Joseph A. Bonanno 95. 697/D800 - The Role of Bicarbonate and Carbonic Anhydrase in Lactic Acid Transport Across Corneal
Endothelium T.T. Nguyen, C. Liu, J. Bonanno. Optometry, Indiana University, Bloomington, IN. ARVO
2010.
96. 2825/D895 - Soluble Adenylyl Cyclase-Mediated Protection of Bovine Corneal Endothelial Cells S. Li, K.
Tan, J.A. Bonanno. School of Optometry, Indiana University, Bloomington, IN. ARVO 2010.
97. 274/D738 Distribution of Lactate Dehydrogenase Isoforms in Bovine Corneal Epithelium and
Endothelium. Tracy T. Nguyen1, Kenichi Iwadate2, Shimin Li1, Joseph A. Bonanno1. ARVO 2011
98. 407/D1054 Investigation of Ion Transport Function of NaBC1 Supriya S. Jalimarada1, Diego G.
Ogando1, Eranga N. Vithana2, Joseph A. Bonanno. ARVO 2011
99. 1092/D716 Oxygen-Deficient Metabolism And Corneal Edema Brian K. Leung1, Joseph A. Bonanno2,
Clayton J. Radke. ARVO 2011
100. 1995/D976 2-Arachidonoylglycerol (2-AG) Induces Corneal Epithelial Cell Migration via
Cannabinoid CB1 Receptors Shimin Li1A, Sherry Hu1B, Douglas McHugh1B, Alex Straiker1B, Joseph A.
Bonanno. Arvo 2011
101. 5483/A284 Inner-retinal Function During And After Acute Elevation Of Intraocular Pressure
Verleen K. McSween1A, Suresh Viswanathan1B, Joseph A. Bonanno. ARVO 2011.
102. 6455/D919 Effect of Boric Acid on Cell Proliferation and Reactive Oxygen Species Production
Diego G. Ogando, Supriya S. Jalimarada, Cailing Liu, Joseph A. Bonanno. ARVO 2011.
103. 6456/D920 Effect of SOD2 Knockdown on ROS Production in Rabbit Corneal Endothelial Cells.
Cailing Liu, Diego Ogando, Joseph Bonanno. ARVO 2011.
104. #1736, Spontaneous And Telomerase Immortalization Of Primary Human Corneal Endothelial
Cells. Thore Schmedt, Yuming Chen, Shimin Li, Joseph A. Bonanno2, Ula V. Jurkunas. ARVO 2012.
105. #1957, Longitudinal Measurement Of In Vivo Fluorescence In Retrogradely Labeled Retinal
Ganglion Cells. Verleen K. McSween, Suresh Viswanathan, Joseph A. Bonanno, Tracy T. Nguyen, Shimin
Li, ARVO 2012.
106. #5335, Lack of Bicarbonate Transport by SLC4A11 in Bovine Corneal Endothelial Cells. Supriya S.
Jalimarada, Eranga Vithana, Joseph Bonanno, ARVO 2012
107. #6005, Functional Study of SLC4A11 in HEK293. cells Diego G. Ogando1, Supriya S. Jalimarada1,
Eranga N. Vithana2, Joseph A. Bonanno, ARVO 2012
108. #6009, Expression Required for Lactate Transporters MCT1 and MCT4 in Rabbit Corneal
Endothelium. Shimin Li, Tracy T. Nguyen, Joseph A. Bonanno, ARVO 2012.
21 Joseph A. Bonanno 109. #6106, Rapid Measurement of Tear Oxygen Tension underneath Soft Contact Lenses by FrequencyDomain Phosphorimetry. Sangly P. Srinivas1, Giovanna Guidoboni2, Lucia Carichino2, Yiran Jiang1,
Joseph A. Bonanno, ARVO 2012.
110. #1658 - D0293. CD147 Knockdown Decreases Corneal Lactate Transport and Endothelial Cell
Viability Shimin Li; Tracy T. Nguyen; Joseph A. Bonanno, ARVO 2013
111. #1675 - D0310. SLC4A11 is an EIPA-sensitive Na+-Dependent pHi Regulator Diego G. Ogando;
Supriya S. Jalimarada; Eranga N. Vithana; Joseph A. Bonanno, ARVO 2013
112. #6324 - D0195. Intracellular pH of Retinal Ganglion Cells During Systemic Hypoxia Verleen K.
McSween; Suresh Viswanathan; Joseph A. Bonanno; Stephen A. Burns; Shimin Li, ARVO 2013
22