GELF POD INCUBATOR OPERATIONS MANUAL FOR MODEL GELF601 PO Box 715 Marietta, OH 45750 800-648-3042 740-373-6809 Fax 740-374-3760 www.caronproducts.com service@caronproducts.com Dear Valued Customer: Thank you for purchasing CARON Products & Services equipment. We appreciate your business and look forward to being your preferred supplier of controlled environment equipment products in the future. At CARON, we are committed to continuous quality improvement. Our goal is to supply our customers with highly reliable equipment at a fair price. In order to openly monitor our performance, we would appreciate your feedback on our products and services. If you have questions, or any suggestions for improvement based on the installation or operation of the equipment you have purchased, please contact our service department at service@caronproducts.com or 740-373-6809. Thanks again for your business! GELF601 Operations Manual Page 2 of 16 Rev A 01/29/13 TABLE OF CONTENTS Section 1 – Warranty Information ..................................................................... 4 Section 2 – Equipment Overview ...................................................................... 7 Section 3 – Equipment Installation ................................................................... 8 Unpacking Choosing a Location Preliminary Cleaning Adjust Door Tension Connecting a CO2 Supply Connecting Electrical Power Section 4 – Equipment Operation ................................................................... 12 Setting the CO2 flow-rate Gelf Pod Section 5 – Preventive Maintenance .............................................................. 14 Section 6 – Specifications ............................................................................... 15 Section 7 – Electrical Schematic .................................................................... 16 GELF601 Operations Manual Page 3 of 16 Rev A 01/29/13 SECTION 1- WARRANTY INFORMATION EQUIPMENT LIMITED WARRANTY Please review this section before requesting warranty service. At CARON, one of our primary goals is to provide customers with high levels of personal service and top quality products, delivered on time, backed by technical service and supported for the life of the product. Before contacting us for warranty service, please be aware that there are repairs that are not covered under warranty. WARRANTY DEFINED Caron Products & Services, Inc. (herein after CARON) hereby warrants that equipment manufactured by CARON is free from defects in materials and workmanship when the equipment is used under normal operating conditions in accordance with the instructions provided by CARON. COVERED: • Parts and labor for a period of two (2) years from date of shipment. • Any part found defective will be either repaired or replaced at CARON's discretion, free of charge, by CARON in Marietta, OH. Parts that are replaced will become the property of CARON. • If CARON factory service personnel determine that the customer's unit requires further service, dependent of the model involved, CARON may, at its sole discretion, provide a service technician to correct the problem, or require the return of the equipment to the factory or authorized service depot. • CARON will have the right to inspect the equipment and determine the repairs or replacement parts necessary. The customer will be notified, within a reasonable time after inspection, of any costs incurred that are not covered by this warranty prior to initiation of any such repairs. NOT COVERED: • Improper installation; including electrical service, gas and water supply tubing, gas supplies, room ventilation, unit leveling, facility structural inadequacies or ambient conditions that are out of specification. • Cost of express shipment of equipment or parts. • Any customer modifications of this equipment, or any repairs undertaken without the prior written consent of CARON, will render this limited warranty void. • CARON is not responsible for consequential, incidental or special damages; whether shipping damage or damages that may occur during transfer to the customer’s point of use. When the equipment is signed for at the customer’s site, ownership is transferred to the customer. Any damage claims against the shipping company become the responsibility of the customer. • Repairs necessary because of the equipment being used under other than normal operating conditions or for other than its intended use. • Repair due to the customer's failure to follow normal maintenance instructions. • Parts considered consumable; including: light bulbs, filters, gases, etc. • Damage from chemicals or cleaning agents detrimental to equipment materials. • Force Majeure or Acts of God. This writing is a final and complete integration of the agreement between CARON and the customer. CARON makes no other warranties, express or implied, of merchantability, fitness for a particular purpose or otherwise, with respect to the goods sold under this agreement. This warranty cannot be altered unless CARON agrees to an alteration in writing and expressly stated herein shall be recognized to vary or modify this contract. Ohio Law governs this warranty. GELF601 Operations Manual Page 4 of 16 Rev A 01/29/13 EQUIPMENT INTERNATIONAL LIMITED WARRANTY Please review this section before requesting warranty service. At CARON, one of our primary goals is to provide customers with high levels of personal service and top quality products, delivered on time, backed by technical service and supported for the life of the product. Before contacting your distributor for warranty service, please be aware that there are repairs that are not covered under warranty. WARRANTY DEFINED Caron Products & Services, Inc. (herein after CARON) hereby warrants that equipment manufactured by CARON is free from defects in materials and workmanship when the equipment is used under normal operating conditions in accordance with the instructions provided by CARON. COVERED: • Parts for a period of two (2) years from date of shipment. • Any part found defective will be either repaired or replaced at CARON's or their authorized representative’s discretion. Parts that are replaced will become the property of CARON. • If CARON or their authorized representatives determine that the customer's unit requires further service, CARON or the representative may, at its sole discretion, provide a service technician to correct the problem, or require the return of the equipment to the an authorized service depot. • CARON or their authorized representative will have the right to inspect the equipment and determine the repairs or replacement parts necessary. The customer will be notified, within a reasonable time after inspection, of any costs incurred that are not covered by this warranty prior to initiation of any such repairs. NOT COVERED: • Calibration of control parameters. • Improper installation; including electrical service, gas and water supply tubing, gas supplies, room ventilation, unit leveling, facility structural inadequacies or ambient conditions that are out of specification. • Cost of express shipment of equipment or parts. • Any customer modifications of this equipment, or any repairs undertaken without the prior written consent of CARON, will render this limited warranty void. • CARON and their representative are not responsible for consequential, incidental or special damages; whether shipping damage or damages that may occur during transfer to the customer’s point of use. When the equipment is signed for at the customer’s site, ownership is transferred to the customer. Any damage claims against the shipping company become the responsibility of the customer. • Repairs necessary because of the equipment being used under other than normal operating conditions or for other than its intended use. • Repair due to the customer's failure to follow normal maintenance instructions. • Parts considered consumable; including: light bulbs, filters, gases, etc. • Damage from use of improper water quality. • Damage from chemicals or cleaning agents detrimental to equipment materials. • Force Majeure or Acts of God. This writing is a final and complete integration of the agreement between CARON and the customer. CARON makes no other warranties, express or implied, of merchantability, fitness for a particular purpose or otherwise, with respect to the goods sold under this agreement. This warranty cannot be altered unless CARON agrees to an alteration in writing and expressly stated herein shall be recognized to vary or modify this contract. Ohio Law governs this warranty. Caron Products & Services, Inc. PO Box 715 · Marietta, OH 45750 740-373-6809 GELF601 Operations Manual Page 5 of 16 Rev A 01/29/13 INTERNATIONAL SYMBOLS AND DEFINITIONS Danger Carbon Dioxide Danger of suffocation (asphyxiate), deficient in oxygen WARNINGS Local government may require proper disposal GELF601 Operations Manual Page 6 of 16 Rev A 01/29/13 SECTION 2 – EQUIPMENT OVERVIEW Congratulations! You have just purchased the latest technology in Gelf Pod Incubators. Before using the equipment, familiarize yourself with key components of the product and thoroughly read this manual. Power switch Cell culture vessels (not included) CO2 flow meter Door handle Removable Gelf Pod GELF601 Operations Manual Power ‘on’ indicator green lights Page 7 of 16 Rev A 01/29/13 SECTION 3 – EQUIPMENT INSTALLATION Unpacking Your new unit has been thoroughly packaged to avoid shipping damage. However, the unit should be fully inspected upon arrival before signing for receipt. If the package has visual damage, make notes accordingly on the freight bill and have it signed by the delivery company. In the event of concealed damage after the unit is uncrated, keep the carton and packaging material. Call the shipping company within 7 days of receipt, request inspection and retain a copy of the inspection report. Remove all foam protective pieces from both outside and inside the incubator. Remove any tape holding components in position during shipping. A shipping kit is also included inside the incubator. The shipping kit includes a user’s manual, electrical cord, & gas connection tubing. Choosing a Location To ensure proper operation, the unit must be located on a firm level surface. The unit should be located in an 18°C – 25°C ambient and out of direct sunlight. A typical location is on a lab bench or in a Bio Safety Cabinet. The unit requires an electrical connection and a regulated pressurized CO2 supply to operate properly. Choose a location where these facilities are or can be made available. Preliminary Cleaning Your new incubator was thoroughly cleaned prior to leaving the factory. It is recommended however, to disinfect all interior surfaces with a general purpose laboratory cleaning agent prior to using the product. After cleaning, dry all interior components with a sterile cloth as necessary. Chloride containing cleaners can attack and corrode stainless steel. Cleaners with strong acids can damage plastic components. GELF601 Operations Manual Page 8 of 16 Rev A 01/29/13 Clean copper surfaces with a cleaning solution approved for copper surfaces such as Gelf Copper Cleaner (GELFKIT). See cleaning section of manual or contact CARON customer service for details. Adjust door tension Raise the door to the most open position. The door should be easily opened and remain open without falling. If the door is too stiff when opening, use a Phillips screw driver and turn the two hinge adjustment screws counter-clockwise. If the door falls closed when letting go, turn the two hinge adjustment screws clockwise. Connecting a CO2 supply High concentrations of carbon dioxide can cause asphyxiation. The use of CO2 monitors and alarms is recommended for areas where CO2 can collect. The CO2 gas supply should be 99.5% pure and should not contain a siphon tube. Install a 2 stage gas pressure regulator with a maximum adjustment of 25 psi. Inlet pressure must be regulated to 15 psi. CO2 tank regulators are available to purchase through CARON customer service. Once the cylinder regulator is installed, connect the outlet of the regulator to the hose barb fitting using the tubing and clamps provided. Check the connections closely for leaks. GELF601 Operations Manual Page 9 of 16 Rev A 01/29/13 CO2 connection Rear view of unit Electrical connection Connecting Electrical Power Ensure that there is unobstructed access to the main power plug disconnect. The incubator runs off of 12 VDC. A universal AC power adapter is included which plugs into 115/230 VAC, 50/60 Hz. Also included are U.S. and European connection. As shipped from the factory, the U.S. plug is on the power supply. To change plugs, simply press the button below the prongs of the plug and push straight up on the prongs. U.S. plug adapter European plug adapter Push here to release plug The unit requires a dedicated electrical outlet. See specific power required and connection. GELF601 Operations Manual Push straight up on prongs to remove plug table below for model Page 10 of 16 Rev A 01/29/13 Plug U.S. Plug European Plug Power Requirements 115V, 60Hz, 16A FLA 230V, 50Hz, 8A FLA Plug Connection NEMA 5-20 CEE 7/7 When the required electrical connection is available, plug the provided power cord into the unit and the electrical outlet. GELF601 Operations Manual Page 11 of 16 Rev A 01/29/13 SECTION 4 – EQUIPMENT OPERATION Turn on the power switch on the front-left side of the incubator. The Caron logo should illuminate with green LED’s and will be on when the unit is on. The copper plates in the bottom of the incubator will begin to warm up keeping product around 37C. CO2 flow setting Adjust the flow meter for the correct amount of CO2 into the Gelf Pod Incubator. Recommended flow settings are 0.08 LPM if placed on a bench (in open air) and 0.16 LPM when used in a bio-safety cabinet (forced air flow). The CO2 concentration inside the incubator will be approximately 5%. Do not set the flow meter above 0.2 LPM. High concentrations of carbon dioxide can cause asphyxiation. In Bio-safety cabinet: 0.16 LPM On Bench: 0.08 LPM GELF601 Operations Manual Flow adjustment knob Page 12 of 16 Rev A 01/29/13 Gelf Pod The Gelf pods are intended to be placed on the shelf of any incubator. The incubator is kept at a controlled temperature and the pod reaches that temperature. The Gelf pod has a large thermal storage capacity and helps maintain the desired temperature during door openings and power outages. An additional purpose of the Gelf pod, is to maintain product at the incubator temperature even when removed from the incubator. The pod will keep product warm for a few minutes when placed on a bench. When used in conjunction with the Gelf incubator, the pod keeps product warm for longer periods of time. This would include processes such as feeding & harvesting cells performed in a bio-safety cabinet. The gelf pods are multi purposed both in how they are used and the cell culture vessels that they hold. They are designed to hold a wide range of sizes of t-flasks, Petri dishes and micro titer plates on them. The gelf incubator will hold product stacked 4.5 inches (11.5 cm). The pod top inside dimensions for placing product is 5 in x 17 in (13 cm x 43 cm). GELF601 Operations Manual Page 13 of 16 Rev A 01/29/13 SECTION 5 – PREVENTATIVE MAINTENANCE Your CARON incubator is robustly designed to minimize performance problems. However, regular maintenance is very important for continuous trouble free operation. Recommended Daily Maintenance Checks Check the CO2 tank levels CO2 flow meter setting Recommended Monthly Maintenance Checks Verify Gelf incubator copper heating plates are warm Gelf Incubator door stays open. Adjust door hinge tension screws if necessary. Recommended Annual Maintenance Checks Disinfect all surfaces with a general purpose laboratory cleaning agent. Clean copper with an approved copper cleaner such as Gelf Copper Cleaner Perform checks of the temperature and CO2 systems. GELF601 Operations Manual Page 14 of 16 Rev A 01/29/13 SECTION 6 – SPECIFICATIONS Temperature CO2 Exterior Construction Interior Dimensions Exterior Dimensions Electrical Unit Weight Surface Construction Insulation Dimensions Unit Weight GELF601 Operations Manual Gelf Pod Incubator Around 37°C Metered Stainless steel with clear, acrylic door 6.4” D x 5.6” W x 18.3” H (16 cm x 14 cm x 46.5 cm) 20.6” L x 7” W x 7” H (52 cm x 18 cm x 18 cm) 12VDC, 1.5A max 115V/230V 50/60Hz power supply 10 lbs (without pod) Gelf Pod Antimicrobial copper Proprietary gel active insulation formulated for 37°C 17.1” L x 5.3” W x 0.8” H (43 cm x 13 cm x 2 cm) 3 lbs Page 15 of 16 Rev A 01/29/13 SECTION 7 – ELECTRICAL SCHEMATICS GELF601 Operations Manual Page 16 of 16 Rev A 01/29/13