Spring PEPS 410 Sustainable Soil and Plant Health Management Lecture/Lab: F 8:30 am – 12:20 pm Magoon 101B Credits: 2 (1 lecture, 1 lab) Instructor: Dr. Koon-Hui Wang (koonhui@hawaii.edu) Students will: 1) learn about beneficial above and below ground organisms that can promote or protect plant health in agroecosystems. 2) gain hands-on experience in managing plant and soil health through sustainable approaches. 3) practice developing integrated pest management programs or soil conservation plans through field trials, interacting with extension agents/specialists, farmers and NRCS agents. Using outdoor training class rooms, students will have opportunities to learn about: Sustainable crop production Cover cropping Benefits of vermicomposting Insectary planting systems Taro loi nutrient and pest management Mushroom cultivation Week 1 Lecture/Lab/Field Trip Lecture 1: Introduction to the soil food web Lab 1: Learn methods to extract soil organisms (including beneficial and pest species), observe soil organisms under the microscope, recognize soil invertebrates from 5 major trophic groups in the soil food web, compare diversity and richness of soil samples collected from different ecosystems. Section I: Conserving beneficial organisms 2 Lecture 2: Use of cover crops to enhance beneficial soil organisms and suppress pests Lab 2: At Magoon teaching plot, plant 6 different cover crops, learn about techniques to monitor plant and soil health (take soil samples, separate rootknot infected plants from rhizobium colonized roots, recognize healthy vs unhealthy root systems, monitor soil compaction), design an insectary garden, bee hotel and set up pit fall traps for weeks #4 and 5. 3 Lecture 3: Insectary plants (IP) for insect pest management Lab 3: Visit native plant nursery by H1 highway (Guest speaker, DR. Joe DeFrank) to evaluate native plants as insectary crops, learn about installing artificial housing (wasps nesting blocks) to attract beneficials, and later introduce them to an agroecosystem. 4 Lecture 4: Benefits and challenges of conservation tillage. Lab 4: Install an insectary garden – transplant pak choi and corn surrounded by insectary plants as border crops (direct seed cilantro, buckwheat, sunn hemp) using class design developed from Week 2. Install a non-insectary control plot. Build a bee/wasp hotel at Student Organic Farm Training plots. 5 Lecture 5: Irrigation water management Introduce “Checkbook method” for water management at Magoon. Student’s short presentation on agricultural systems that would increase WUE. Learn how to irrigate precisely. Monitor arthropods in pit fall traps using scanner, maintain insectary/non-insectary plots. 6 Lecture 6: Enhancement of beneficial soil microorganisms for plant health improvement: PGPR, mycorrhizae Lab 6: Learn to maintain a vermicompost bin, cure vermicompost, and brew vermicompost tea (VCT). Using the insectary plot established, separate each crop into 6 sections. These sections will be drenched with 1) VCT from cured vermicompost, 2) VCT from uncured vermicompost, 3) VCT from partially cured vermicompost, 4) CT from oyster mushroom compost, 5) water control at weekly intervals; vs 6) soil amended with vermicompost, and 7) mushroom compost at transplanting. Monitor plant height and chlorophyll content periodically. 7 Lecture 7: Enhancement of beneficial soil organisms for inducing host plant resistance against pests: SAR, ISR Lab 7: Monitor insects in the insectary trial, visit different fields at Magoon and SOFT garden for caterpillars and other insects, and inoculated with entomopathogenic nematodes (EPN) to be used for Lab 8. Take home assignment: students will be given a jar to collect a soil sample (not from Magoon) to bait for EPN using 5 meal worm larva and bring the jar back to class the following week. Turn in lab note book (Lab 1-7) for evaluation. Section II: Augmentation of beneficial organisms 8 Lecture 8: Entomopathogenic nematodes (EPN) for insect control. Student short presentation to share successful EPN projects throughout the world. Lab 8: Visit Nematology Lab (Dr. Brent Sipes) to learn EPN rearing techniques, set up white trap for insect larva inoculated last week, learn about EPN distribution in Hawaii, monitor EPN for the next two weeks. 9 Lecture 9: Mushroom cultivation and use of mushroom compost. Student short presentation to share different benefits of mushroom and its cultivation. Lab 9: Count EPN collected from Lab 8. Monitor plant height and chlorophyll content from Lab 6. Monitor insects in the insectary trial. Monitor bees/wasps hotel, and wasps nesting blocks. 10 Lecture 10: Natural Farming concepts: Fukuoka Natural Farming, permaculture, veganic culture, Korean Natural Farming (KNF), biointensive organic farming. Lab 10: Prepare IMO 1-4, practice to use KNF calculator to prepare KNF foliar inputs, Type II, Type III, SOS, SES solutions, and how to prepare nutrient inputs using farm waste. Count EPN from Lab 8. 11 Lecture 11: Potential of biological control agents or biological based pesticide against plant-parasitic nematodes. Lab 11: Observe natural enemies of nematodes under microscope -- Predatory nematodes, nematode-trapping fungi, nematode egg parasite, Pasturia. Collect natural farming nutrient inputs prepared on week 10, harvest cover crop biomass from cover crop plots, dry in oven to get dry biomass. Learn to use Cover Crop Calculator to estimate plant available nutrients (PAN) from cover crop residues. Section III: Integrated approaches 12 Lab 12: Field trip Visit commercial loi (wetland taro), practice problem solving in aquatic crop production (a scenario where no pesticide is allowed) with a soil salinity issue. All students must write a 2 page suggestion in their lab note book on how to recommend plant and soil health management for this farm based on their observations and interview with farmer. Use all the information you learned from previous lectures. Please type (12 point font, single line spacing) and print it out, paste it in your note book. 13 Good Friday 14 Lecture 13: Integrated root disease management approaches for organic farming Lab 13: Wrap up all data collection, work with instructor and TA for data analysis. 15 Student team project presentation. Turn in lab note book (Lab 8-13). Final Exam