Can LEDs image ethidium bromide?
11/10/15, 2:53 PM
Blue/Green LED Gel Imaging
System Sees ALL Stains Equally
Hello Again from Bulldog Bio!
We'd typically be contacting you during the second half of August with
our popular Once Bitten promotional offerings. Because we've such a
log jam of recent product launches, we've decided you'd be more
interested in learning what's new at Bulldog.
Nippon Genetics offers a broad range of products with a special focus
on tools and reagents for electrophoresis. Below is a premium
upgrade to their wildly popular GelPic Blue LED Gel Imaging System.
See what makes it so special.
Best In Show
GelPic Blue/Green LED Gel Imaging System
TheFastGene GelPic
Blue/Green LED System is the
next step forward for
inexpensive and safe gel
documentation. Improving upon
the already popular GelPic Blue
LED System with a longer
excitation wave length gives
brilliant results for ethidium
bromide and other safe red
PCR enzyme for under 2-cents
per reaction!!!
Throw Me A Bone
BioReady rTaq is one of our
oldest and most beloved
reagent lines. Inexpensive
PCR enzyme is only good
when it works. Years of repeat
sales attest to the quality of this
product. Special 50% discount
on your first 5,000 units of rTaq
is an insanely good price!
That's 5,000 units for only $75
or only 1.5 cents for a unit of
this enzyme - which also
includes the 10x Running
Buffer and 6x Loading Dye. If
you've never tried this basic
PCR enzyme we'd be happy to
supply you with a free sample
to test. How can you go wrong?
Use promo code
BDB1508RTAQ to receive this
offer. [good through September
Page 1 of 5
Can LEDs image ethidium bromide?
DNA dyes. In fact, the results
are indistinguishable from the
best UV transilluminators on
the market. The Blue/Green
LEDs also provide remarkably
bright results for popular safe
green dyes, such as our own
Midori Green DNA Stain . The
camera was also upgraded to
provide higher resolution
pictures and export in multiple
file formats -- including TIFF
files. And like the original
system, the GelPic Blue/Green
has white LEDs for imaging
Same small box as the original GelPic
Coomassie- or silver-stained
gels. It also boasts overhead
white LEDs for coloretric-stained western blots. Even a petri dish
loaded with colonies is a click away.
11/10/15, 2:53 PM
2015]
DOES ANYONE WANT TO
WIN A FREE Gel Imaging
System? We've received a
remarkably small number of
entrants. Use the contest
code BDB1508GELPIC when
entering the contest for a
great chance for a GelPic
LED Imaging System ($2995
value).
Featured Products
FastGene GelPic Blue/Green
LED System
Related Product
LED Illuminators and Gel
Imaging Systems LED-based
instruments from Nippon
Genetics
Midori Green DNA Gel Stain
safe for DNA and safe for your
lab
Ethidium bromide staining looks great with blue/green LEDs
All settings and imaging happen at a simple interface on the front of
the system - so you won't need a dedicated computer to drive this
system. Its "copier-style" platen even allows you to excise bands for
subcloning. This versatile documentation system has a shockingly low
price of only $3995. Contact us now with this Barker-only Promo code
BDB1508 GELPICBG and receive an automatic discount of $500 to
make it that much more affordable. [Offer good through September
2015]
AcquaStain Protein Gel Stain
1-step and 10 minutes to see
your bands
Fastgene Agarose Gel Band
Cutter the simplest way to
excise DNA bands
Fastgene Agarose Gel Tablets
1-step gel pouring
ADVANTAGES:
Visualizes and saves images from gels, blots and more
New LED technology works great with ethidium bromide
Better camera mechanics and file format
Starts at under $4,000
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Can LEDs image ethidium bromide?
11/10/15, 2:53 PM
A Dog's Life "real lab bench experience"
All gel documentation systems are simply
tiny photography studios -- a camera,
stands, backdrop and lighting. So why not
use this miniaturized set-up for more than
just gels? It makes sense in this climate of
diminishing lab budgets! The FastGene
Gel Pic LED Imaging System is designed
exactly for this purpose. It can capture
images of blots, colorimetric paper-based
assays or anything else that reflects lightwaves effectively with overhead white LED
illumination and requires full-color high
resolution imaging. The backlit white LED
is ideal for experimental results that are
transparent or translucent in nature. This
light passes through gel matrices as well
as clear petri dishes or agar media for
Blue-white colony selection captured by the GelPic
crystal clear documentation of viral
plaques or blue/white colony selection.
The backlit blue LED mode is ideal for assays such as end-point PCR checks when the samples are
stained with fluorescent dyes in the green range such as Sybr or Midori Green. Versatility equals cost
savings for this small, inexpensive imaging system.
Cloning of a DNA fragment into a plasmid followed by
blue/white colony screening is a near sacred ritual performed
in nearly every life science lab throughout the world. But
surprisingly few researchers understand the true impact of UV
light and intercalating dyes on the efficiency of such a
venerated application. As can be seen below, the common
DNA stain, ethidium bromide, leads to far less efficient cloning
as compared to newer classes of dyes such as Midori Green.
Midori Green was developed to be far less harmful to the
environment and lab workers. The underlying root mechanism
that has led to this safe stain also provides cloning efficiencies
nearly triple that of ethidium bromide.
Ethidium bromide is typically used in conjunction with a
Sybr Green stained qPCR plate. Note well
8B is negative.
Page 3 of 5
Can LEDs image ethidium bromide?
11/10/15, 2:53 PM
UV Transilluminators Kill Cloning Experiments
strong UV light source to excise DNA bands for purification
prior to the ligation reaction. Short wave-length light is well
known to cause thymidine dimers and damage the DNA.
The extent of this damage is not always appreciated. High
energy light wreaks havoc on a DNA fragment in mere
seconds. As can be seen below, after only a 15 sec
exposure of DNA in a standard agarose gel, cloning
efficiency starts to drop. And after a 30 sec exposure your
cloning experiment is all but dead! In contrast, the cloning
efficiency of protocols that use blue or blue/green LEDs
seem completely immune to any deleterious effects. If your
lab isn't able to break itself of its ethidium bromide habit,
using a blue/green LED illuminator (and imaging systems)
have no significant impact on cloning efficiency or DNA
integrity. When combining Midori Green with blue/green
LED illumination your lab will be provided the promise of
good health, safe environment and the best results
possible.
Midori Green Can Also Boost Your Cloning
Results!
Midori Green DNA Gel Stain is a non-toxic alternative to ethidium bromide for use in DNA gels and other
common applications. It now has over 150 citations in the recent scientific literature. Below is a link to a
2015 online research article from Indiana University - Purdue University Indianapolis. We're always
grateful to every lab that takes the time to reference our products!
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Can LEDs image ethidium bromide?
11/10/15, 2:53 PM
Midori Green DNA Stain:
Mutational Analysis of the Chlamydia muridarum Plasticity Zone
K Rajaram, AM Giebel, E Toh, S Hu, JH Newman, SG Morrison, L Kari, RP Morrison, & DE Nelson
(2015). Infection and immunity, IAI-00106.
K9 Logic "Puzzle of the Month"
Once again, we'll be giving away 30 free GIANTmicrobe prizes to those of you who correctly answer the
K9 Logic puzzle presented below. We have now two quarters in which many of you provided correct
answers. Let's see if we can keep this streak alive for the third quarter of 2015 (July, August and
September). Click here to submit your answer and best of luck.
Four species of bacteria were tested for resistance to a new antibiotic.
Unfortunately this key experiment was left to an undergraduate to run,
and as can happen, the undergrad mislabeled the plates. The student
was, however, able to garner just enough correct information to determine
the order in which the four species showed resistance to the new
antibiotic. Please read the clues below and let us know the correct genus
and species of each bacteria and the order from most resistant to least
resistant bacteria.
Who knew DNA could make
such a weird looking stuffed
toy?!
Escherichia coli was more resistant than Helicobacter. Enterica was more
resistant than Shigella. Salmonella was not the third most resistant.
Dysenteriae was not the least resistant. Baculiformis, which was not the species of Salmonella, was
slightly less resistant than Escherichia.
.
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