For life science research only. 
Not for use in diagnostic procedures.
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Bovine Serum Albumin, Fraction V, 
fatty acid free
from bovine serum
Lyophilizate
Cat. No. 10 775 835 001
50 g
y Version 05
Content version: January 2005
Store at +2 to +8°C
Introduction
Albumin reversibly binds many substances and can
assume transport and vehicle functions in the body.
Albumin’s ability to bind a number of anions and cations can be used to study ion binding. The affinity of
albumin for ligands depends on the hydrophobic character of the molecules and their charge. Molecules
with long alkyl chains and negatively charged groups
are bound very firmly, while molecules with short
chains and positively charged groups are bound less
firmly (1).
Ligands bound by albumin include fatty acids, cationic
and neutral detergents, acetylcholine, ascorbic acid,
penicillin, thyroxin, digitonin, hormones, metal ions, bilirubin, sugars and drugs (2). The most important function of albumin in the body is the transport of lipids and
free fatty acids. Albumin, free from fatty acids, is used
to investigate these processes.
Stability
At +2 to +8°C, the lyophilizate is stable until the expiration date specified on the label. In solution, albumin is
stable for about 3 months at ⫺15 to ⫺25°C.
Solubility
Albumin is readily soluble at concentrations of 50 to
100 mg/ml. For best results, add a non-ionic detergent
to avoid the formation of foam when preparing solutions (gentle stirring).
References
1 Spector, A.A., Fletcher, J.E. & Ashbrook, J.D. (1971) Biochemistry 10, 3229.
2 Skipski, V.P. (1972) in: Blood Lipids and Lipoproteins: Quantitation, Composition and
Metabolism (Nelson, G.J. ed.) New York, Wiley-Interscience.
3 Hirayama, K. Akashi, S., Furuya, M. & Fukuhara, K. (1990) Biochem. Biophys. Res.
Commun. 173, 639.
4 Saint-Blancard, A.M. (1973) J. Ann. Pharm. Fr. 31, 513.
5 Chen, R.F. (1967) J. Biol. Chem. 242, 174.
Changes to previous version
Editorial changes
Application
Albumin, fraction V, free from fatty acids, is used in
lipid research:
• to study lipid metabolism in biological systems,
• to identify the transport mechanism in living
organisms,
• to study ligand binding,
• to investigate ionic binding.
Product
advantages
• Free from fatty acids according to current quality
control procedures (see Quality control below) 
(suitable for lipid research)
• High quality (see quality control parameters below)
• Low content of heavy metal ions 
(Efficient binding to secondary protein structures;
the biological reactivity of the material is unaffected)
• Long-term stability guaranteed (see label)
Ordering Information
Roche Applied Science offers a large selection of
reagents and systems for life science research. For a
complete overview of related products and manuals,
please visit and bookmark our Homepage, www.rocheapplied-science.com
Product
Bovine Serum Albumin, Fraction V
Bovine Serum Albumin, solution, 
special quality for molecular biology
pH
7.0 ± 0.2
Analysis
Albumin (electrophoresis)
> 99%
Protein
> 98%
< 5%
H2O
Fatty acids (total)
< 0.2 mg/g
Triglycerides (enzym.)
not detectable
Immunoglobulins (Ouchterlony) not detectable
Sodium (flame photometry)
< 0.5%
Potassium (flame photometry)
< 0.01%
Calcium (AAS)
< 0.02%
Magnesium (AAS)
< 0.01%
Iron (AAS)
< 0.001%
Copper (AAS)
< 0.002%
P (inorganic)
< 0.001%
Heavy metals (as Pb)
< 0.001%
Albumin, fraction V, free from fatty acids, is
manufactured using a modification of the methods 
of Saint-Blancard (4) and Chen (5).
1011.11743902001➄
Pack size
50 g
100 g
500 g
1 kg
20 mg
(1 ml)
Please refer to our latest catalogue or Homepage for
our current BSA product range, or contact your local
Roche representative.
Product description
Molecular weight 68,000 Da (583 amino acids; see Ref. 3 for amino acid
sequence)
Cat. No.
10 735 078 001
10 735 086 001
10 735 094 001
10 735 108 001
10 711 454 001
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their respective owners
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