Using acoustic Doppler velocimeter backscatter response to determine suspended sediment concentration Laura Tait Amherst College Mentors: Carl Friedrichs and Grace Cartwright August 3rd, 2012 Synopsis: This study revealed both Nortek and Sontek acoustic Doppler velocimeters (ADV) responded within the interinstrument variation for each manufacturer with a slight downward bias in acoustic response, measured in a constant mud concentration, to the application of anti-fouling paint on the sensors. The accuracy in the sampling methodology used in this study was calculated to be within 1% of the burst mean acoustic response measured for each manufacturer. Both the Nortek and Sontek ADVs produced the expected increase of backscatter counts linearly proportional to the log of sand concentration up to about 500 mg/L. The calibrations revealed the 10 MHz Nortek was less sensitive to the 130-150 µm sand than the 5 MHz Sontek recording less backscatter counts for each concentration measured. Backscatter counts began to increase less quickly with the log of sand concentration at about 800 mg/L for both instruments and decreased for the 10 MHz case at about 1000 mg/L. Abstract: The first of two principle objectives of this research was to quantify variability in the backscatter measured by the ADVs, first between instruments, and then within the sampling procedure itself. The inter-instrument variation in the acoustic backscatter response for each of the ADV manufacturers, Nortek (10 MHz) and Sontek (5 MHz) reveled burst average variation between the instruments was on the order of the interburst variation, except for the higher response by a single newly repaired Sontek ADV. Application of anti-fouling paint was found to produce a slight downward bias which was well within the inter-instrument variation determined for each manufacturer. A method quantitation limit, a measure of the level of accuracy of the burst mean acoustic response, determined both ADV models are accurate to within 1% or less. The final objective was to investigate the changes in the regression of backscatter response versus increasing concentrations of sand (130-150 µm) for several frequencies (for this paper, only the 5 and 10 MHz cases from the ADVs are considered). The ADVs responded linearly as expected to increasing sand concentrations, recording higher backscatter for higher concentrations up to about 500 mg/L. The 5 MHz Sontek ADV sensor was more sensitive than the 10 MHz Nortek, giving a higher backscatter response for each concentration measured, as expected, due to the frequency’s higher sensitivity to sand grainsize. Introduction: Sediment transport is an important process that greatly affects the geomorphology of coastal environments such as estuaries (Friedrichs et al. 2008). Yet the implications of this movement of sediment extend beyond only physical changes. Because particles can be a source of both nutrients and toxic material like pollutants, suspended sediment transport can distribute these, thus exerting major control on estuarine water quality (Gartner 2004, Friedrichs et al. 2008, MacDonald et al. 2012). In limiting light transmission, suspended sediment can also influence photosynthesis, and deposition of this sediment can potentially render shipping channels not navigable (Gartner 2004). By their very nature, estuarine environments are constantly changing. Aggregate sizes, suspended sediment concentration and settling velocities can often shift on short time scales, making it difficult to study the condition of these highly variable systems (Fugate and Friedrichs 2003, Gartner 2004). A better understanding of sediment transport processes is vital for better understanding nearshore sedimentary processes (Vousdoukas et al. 2011). One crucial part of sediment transport that needs to be better understood and measured is the concentration of suspended solids. Correctly estimating sediment concentration can be a difficult feat, particularly in estuaries, where the sediment grain size distribution changes tidally as well as seasonally (Cartwright et al. 2011). Although originally intended to measure velocities, the acoustic Doppler velocimeter (ADV) is one instrument that is being used to determine suspended sediment concentration (Fugate and Friedrichs 2002, Cartwright et al. 2012). An estimate of the concentration is made by relating measured acoustic backscatter “counts” to sediment concentration. This can often be aided by laboratory calibrations that build an empirical relationship between the backscatter intensity and sediment concentration (Thorne et al. 1993, Voulgaris and Meyers 2004, Cartwright et al. 2009, MacDonald et al. 2012). By emitting sound waves of a known frequency and recording the backscatter, ADVs are able to reasonably estimate the concentration of suspended sediment in water. The data collected by these instruments are invaluable in obtaining long-term concentration estimates, which are essential for learning more about estuarine processes (Cartwright et al. 2009). While it is true that optical instruments may also be utilized to estimate suspended sediment concentration, the way they detect suspended sediment differs from acoustics in several ways. Optical methodology operates by measuring light backscattered from the surface area of sediment while acoustics respond to additional properties of the sediment in suspension, such as the density contrast between the sediment and surrounding water. This means that optical instruments are more sensitive to fine-grain sediment, producing a greater response for mud relative to the same concentration of sand because mud flocs have higher surface area than sand. Acoustics, on the other hand, are less sensitive to mud, giving a smaller response to mud flocs for the same concentration of sand because sand produces a stronger density contrast with water than flocs do. One major drawback of using optical sensors is they are much more susceptible to biological fouling, meaning that acoustic sensors can be deployed for longer periods of time (Gartner 2004). Previous studies suggested the advantage of using ADVs and other acoustic instruments is that they are relatively insensitive to aggregate size, responding to constituent grains rather than the aggregate as a whole (Fugate and Friedrichs 2002, Scully and Friedrichs 2007). However, MacDonald et al. (2012) propose that acoustic sensors actually do respond to the floc itself, not the components, thus complicating the role of acoustics in determining suspended sediment concentrations. Although relatively more is understood about how acoustics respond to sand particles in suspension, little is known about how they respond to finegrain particles, especially when the grains bond naturally to form flocs and/or fecal pellets or when they are part of a natural mixed grain size distribution of suspended sediment. The goal of this study was twofold. First, the acoustic response of ADVs to a known concentration of fine sediment (less than 63 microns) was tested with and without the application of antifouling paint on the sensors. This compared results between instruments of the same frequency and investigated whether or not the antifouling paint affected the acoustic backscatter recorded by the ADVs. This is necessary to determine what the variability is between instruments used for the long-term monitoring of suspended sediment concentration for the National Science Foundation MUDBED (Multi-Disciplinary Benthic Exchange Dynamics) project. The second part of this research involved conducting an acoustic regression for a known size distribution of sand (150180 microns) at different concentrations. This sand distribution was chosen because it is in region where the acoustic attenuation relationship to grain-size and frequency is in the much better understood area where multiple scattering and particle-particle interactions tend to dominate the acoustic attenuation (see Figure 1). The ADVs used for the project are from different manufacturers and operate at different frequencies, so this tested how each frequency responded to the changing concentrations. Additionally, five acoustic transducers that produce raw acoustic response were recorded for the same sand concentrations as the ADVs. ADVs report the acoustic backscatter as “counts,” which can then be converted to approximate sediment concentrations. However, the counts are possibly modified in some way to reduce error in the velocities they were originally designed to measure. The transducers, on the other hand, have no such modification, which allowed a more pure acoustic response to be recorded. This experiment was meant to verify that with a known grainsize concentration range, the instruments respond according to accepted theory that ADV backscatter intensity increases with increasing sediment concentration. Methods: Instrument Inter-comparison: This section addressed the first objective and consisted of two experiments (Run 1 and Run 2). In both experiments ADVs were tested for variation within the same manufacturer (frequency). In Run 1, acoustic response was also measured with and without antifouling paint. Four Nortek ADVs (10 MHz) and four Sontek ADVs (5 MHz), were tested in the first experiment. In the second experiment, one additional ADV from each manufacturer was tested and two from each were repeated again to provide continuity between the first and second experiments and allow comparison of equipment that had been deployed. Bottom sediment collected in the Clay Bank region of the York River, VA was wet sieved using deionized water through a 63-micron sieve and collected as a slurry. The total solids concentration of the slurry was determined by pipetting 20 ml into a weighed dish, dried at 103-105 oC and weighed again. An aliquot of the slurry was then added to the 110-liter calibration chamber (Figure 2) to bring the concentration to approximately 100 mg/L. Clay Bank is the location of long term MUDBED tripod deployments where suspended sediment concentrations in the range of 100 mg/L are frequently estimated using acoustic backscatter from the ADV and in-situ pumped samples (Cartwright et al. 2009). The circulating pump incorporated in the design of the chamber ensured that the concentration was well mixed for the entire experiment to maintain a constant scattering concentration at the sampling volume of the sensors. In the first experiment, the sensors of each ADV were painted with a thin coat of the antifouling paint (Trilux 33) in the same manner as they are for deployment on the Clay Bank tripods (Cartwright et al. 2009). The thickness of the paint was the thinnest layer possible which did not expose the color of the underlying transducer. The ADVs were inserted one at a time into the chamber so that each instrument’s sampling volume was level with the same chamber sampling tube. A “painted” sample burst, a five-minute record with a sampling rate of 8 Hz (Nortek) or 10 Hz (Sontek), was collected for each ADV. All paint was then removed from the sensors and each ADV was re-inserted one at a time into the chamber and a “non-painted” sample burst was collected. The second experiment used only used unpainted ADVs so only a “nonpainted” sample burst was collected for each of the ADVs during this experiment. During the first experiment, in addition to the ADVs, “non-painted” sample bursts were collected with five acoustic transducers (0.5MHz, 1.0 MHz, 2.25 MHz, 5.0 MHz and 10MHz) using UTEX Scientific Instruments INSPECTIONWARE program. These data will not be further considered in this paper but will be used for future analysis. The five transducers were strapped together and placed in the chamber so the ADV sampling volume height was in the middle of the acoustic profile collected one at a time by each transducer. A 2-liter water sample was collected at the end of each experiment by pushing the sample tube that corresponds to the ADV’s sample volume height into the chamber. This allows the sample to be collected from the center of the chamber. These suspended solids samples were filtered through a weighed glass fiber filter (nominal pore size 0.7 𝜇𝑚), dried at 103-105o and then reweighed to determine the total suspended solids (TSS) concentration. All recorded data from both intercomparison experiments were analyzed and graphed using MATLAB. Method Quantitation Limit: To determine the repeatability of the sampling procedure, one Nortek ADV was inserted and removed from the calibration chamber ten times into the same concentration as used for the first inter-comparison experiment. Care was taken to place the instrument sample volume in the same location of the chamber each time the instrument was inserted and a five-minute data “burst” was recorded for each insertion. To compare the variability between manufactures this procedure was repeated for one Sontek ADV. The data were analyzed and graphed with MATLAB. Sand Calibration Study: Quartz sand was passed through a 180-micron sieve and captured on a 150-micron sieve to provide a relatively narrow size distribution of sand for this portion of the study. One Sontek ADV and one Nortek ADV, both unpainted, were mounted in the chamber along with the same series of five acoustic transducers used above, ensuring that the instrument sampling volume was at the same height in the center of the tank corresponding with a chamber sampling tube (see Figure 2b). A series of the aliquots of narrow sand distribution was added to the chamber to bring the expected concentration to approximately 25, 50, 100, 150, 200, 300, 400, 600, 800, 1000, 1200 and 1600 mg/L, respectively. For each concentration the ADVs and transducers were turned on one at a time to prevent cross-talk between the instruments and a five-minute burst was collected. Before the addition of the next sand aliquot, a water sample was collected from the appropriate sampling tube and analyzed for suspended solids concentration by filtering on a weighted glass fiber filter (~07 µm pore size), dried at 103-105 o C. and re-weighted. Pump sampling and filtering for the first half of the sand calibration study was repeated, using only the concentrations from 25 mg/L to 400 mg/L in order to obtain more accurate TSS data. A systematic error had been found in the pump sampling and the filtering procedure for the first set of water samples. Because of the shape of the collection bottles, some sand was caught in the corner of the bottles, and it was difficult to remove enough of the sand for accurate filtering in lowest concentration cases, thus giving erroneous results that were much more evident at the lowest concentrations. These concentrations were repeated in Run 2 using 2 liter graduated cylinders to collect the samples, and they were filtered right away. As before, all data were analyzed and graphed using MATLAB. Results: Instrument Inter-comparison: The measured results of the suspended solids concentration in the calibration chamber was 130 ± 12 and 80 ± 6 mg/L for Runs 1 and 2, respectively. The Nortek burst mean responses for Run 1, with paint, range between 159.2 and 151.7 counts with burst standard deviations about the mean of 1.8 to 1.9 counts. (Figure 3 and Table 1). The mean standard deviations between the three beam responses for each record range between 2.3 and 6.1. Nortek Run 1 responses, without paint range between 160.7 and 153.2 counts. The Run 2 responses range between 151 and 146 counts (slightly lower because of the lower suspended solids concentration). The Sontek burst mean responses for Run 1, with paint, range between 157.7 and 124.2 counts with burst standard deviations about the mean of 3.5 to 4.0 counts (Figure 3 and Table 1). The mean standard deviations between the three beam responses for each record range between 2.9 and 3.9. Sontek Run 1 responses, without paint range between 127.5 and 160.3 counts. The Run 2 responses range between 122.4 and 145 counts (slightly lower because of the lower suspended solids concentration). Method Quantitation Limit (MQL): The Nortek and Sontek ADVs both demonstrated a similar response for the method detection limit and were fairly consistent during the ten runs. The Nortek range of the burst means was 143.1 to 146.1 with a mean of the 10 burst means of 144 counts and a standard deviation about that mean of 0.067 counts (Table 2 and Figure 4). The MQL (3 times standard deviation) was calculated to be 0.199 counts. The Sontek ADV range of burst means was 123.6 to 126.0 with a mean of the burst means of 124 counts, a standard deviation of 0.47 counts and a MQL of 1.41 counts (Table 2 and Figure 5). Sand Calibration Study: Overall, the Nortek and Sontek instruments recorded an increase in backscatter counts corresponding to increased sand concentration. There are no visual upward or downward trends observed in the burst data in time, although it is evident some of the sand was trapped during the circulation process. Figures 6 and 7 show strong linear relationships between the amount of sand added to the chamber and that measured in the water sample collected. The relationship between the log10 expected concentration and the log10 measured concentration was linear with an R2 value of 0.989 (Figure 6). Figure 7 shows only 63% of the expected concentration, based on the amount of sand added to the calibration tank, was actually kept in suspension. Figures 8 and 9 show a steady upward trend between the log10 concentration and the acoustic response until about 500 mg/L. At that concentration the relationship appears to taper off and by 1000 mg/L the Nortek is actually recording a lower response for an increase in concentration (Figure 8). almost the same to painting. In addition, these results demonstrate the potential importance of manufacturing date and repair history in acoustic response. It is possible that, because the five Nortek ADVs were built around the same time, they show a more consistent response across instruments. The outlying Sontek ADV, V20358, is the only Sontek recently repaired, perhaps accounting for its anomalously high response. Further, the other four Sontek instruments are of varying ages and come from different manufacturing batches. This may explain in part why the Sontek ADV responses are more scattered than the Nortek ADVs. The acoustic response of both the Nortek and Sontek ADVs was highly correlated with the log10 concentration of sand in suspension within the linear range of less than 500 mg/L (R2 = 0.969 and 0.982, respectively). The Nortek ADV exhibited a lower sensitivity by recording a lower number of counts for the same concentration as the Sontek. The Nortek ADV also had a smaller range of responses with only 28 counts separating the highest and lowest concentrations in the linear range, while the Sontek ADV had a greater range of 47 counts. The similar results for both Nortek and Sontek ADVs for the MQL signify that both instrument types are generally consistent even when removed and re-inserted into the calibration tank. Therefore any error that may occur from this process is likely small and will not greatly affect the ADV response. However, the fact that the backscatter was generally higher for the 10 MHz Nortek ADVs in both the intercomparison and the method quantitation limit suggests an important role of frequency in acoustic backscatter. Attenuation is frequency-dependent, so for the fine sediment used in these tests, the higher-frequency Nortek ADVs produced a higher response than the Sontek ADVs. Discussion: The instrument inter-comparison tests suggest several key facts about the effect of antifouling paint on acoustic response as well as variation within the same frequency ADVs. Overlapping error bars between painted and non-painted instruments for both Nortek and Sontek indicate that while there is a slightly negative backscatter bias for painted ADVs, in general the difference is not significant enough to strongly skew data. Overall, both instrument types seem to respond Interestingly, for the sand calibration study, on average, the Nortek ADV recorded lower backscatter counts than the Sontek, reinforcing the fact that attenuation and the transition between absorption (mud) and scattering (sand) are dependent on frequency. The general trend of increasing backscatter counts for both instrument types with increasing sand concentration adheres to accepted theory and verifies the acoustic response of both the Nortek and Sontek ADVs. As expected, with increasing sand concentration, both instruments eventually begin to respond less due to the attenuation of acoustic energy by the high sediment concentration. Higher frequencies tend to exhibit effects of attenuation at lower concentrations, so because the Nortek ADV operates at 10 MHz and the Sontek only at 5 MHz, the Nortek response weakens first. This produces the slight decrease in Nortek backscatter intensity at the highest concentration. On the other hand, the Sontek response creates more of a plateau consistent with its lower frequency. The backscatter count range difference between the Nortek and Sontek ADV may also be a result of their frequencies, though it could possibly be merely due to instrument differences. well as for a method quantitation limit. Further, the sand study has allowed for the calibration of Nortek and Sontek ADVs, relating recorded backscatter counts to suspended sediment concentration. This work substantiates the theory that acoustic backscatter can be used to determine sediment concentrations. However, previous studies have shown that acoustic response acts as expected only for sand concentrations while other sediment types, particularly mixed sediments, are less clear. The studies carried out in this experiment set the stage for future work on this subject to better understand how acoustic backscatter from ADVs responds to more complex types of sediment concentrations. The variation between expected and measured sand concentration also points to a significant issue about sampling from the calibration tank. The TSS calculations for mud were not problematic because mud is so easily suspended in water. Heavier sand, on the other hand, is more likely to settle in the chamber and pump, making it more difficult to obtain accurate samples through the sampling tubes. This additional noise added by the sampling combined with error during filtering may account for the discrepancy between expected and measured concentrations. Nevertheless, despite the fact that concentration added and concentration measured did not have a perfect 1:1 ratio, their relationship was still very linear, meaning that although the sand may not be evenly distributed throughout the chamber, the area of acoustic sampling is fairly well-mixed and consistent for each concentration. The resulting calibrations for both Nortek and Sontek produced good linear regressions with a fairly small margin of error in a 95% confidence interval. Acknowledgements: I thank everyone who contributed ideas, technical help, or equipment; particularly my incredible mentors Carl Friedrichs and Grace Cartwright. I also thank Kelsey Fall, Carissa Wilkerson and the rest of the CHSD lab for all their help. Thanks to Drs. Rochelle Seitz and Linda Schaffner for coordinating the Research Experience for Undergraduates program at the Virginia Institute for Marine Science and the National Science Foundation grant OCE-0552612. This work was also made possible by additional funding from NSF, Division of Ocean Sciences, grant OCE-1061781. At the conclusion of this research, all ADVs of both types have been tested for variability between instruments as Literature Cited: Cartwright, G.M., C.T. Friedrichs, P.J. Dickhudt, T. Gass, and F.H. Farmer. 2009. Using the acoustic Doppler velocimeter (ADV) in the MUDBED real-time observing system. Proceedings, OCEANS 2009 MTS/IEEE, CD ISBN: 978-0-933957-38-1, 9 p. Cartwright, G.M., C.T. Friedrichs, and L.P. Sanford. 2011 (in press). In situ characterization of estuarine suspended sediment in the presence of muddy flocs and pellets. N.C. Kraus and J.D. Rosati (eds.), Coastal Sediments 2011, American Society of Civil Engineers, 14 p. Cartwright, G.M., C.T. Friedrichs, and P.D. Panetta. 2012. Dual use of a sediment mixing tank for calibrating acoustic backscatter and direct Doppler measurement of settling velocity. Submitted to: Oceans 2012, MTS/IEEE, Virginia Beach, VA, 14-19 Oct. Friedrichs, C.T., G.M. Cartwright, and P.J. Dickhudt. 2008. Quantifying benthic exchange of fine sediment via continuous, non-invasive measurements of settling velocity and bed erodibility. Oceanography, 21(4): 168172. Fugate, D.C., and C.T. Friedrichs. 2002. Determining concentration and fall velocity of estuarine particle populations using ADV, OBS and LISST. Continental Shelf Research, 22: 1867-1886. Fugate, D.C., and C.T. Friedrichs. 2003. Versatility of the Sontek ADV: measurements of sediment fall velocity, sediment concentration, and TKE production from wave contaminated velocity data. In: R.A. Davis et al. (eds.), Coastal Sediments 2003, ASCE, CD ISBN: 978981-238-422-5, 14 p. Gartner, J.W. 2004. Estimating suspended solids concentrations from backscatter intensity measured by acoustic Doppler current profiler in San Francisco Bay. Marine Geology, 211 (2004): 169–187. MacDonald, I.T., C.E. Vincent, P.D. Thorne, and B.D. Moate. 2012. Acoustic scattering from a suspension of flocculated sediments. Submitted to: Journal of Geophysical Research. Scully, M.E., and C.T. Friedrichs. 2007. Sediment pumping by tidal asymmetry in a partially-mixed estuary. Journal of Geophysical Research, 112, C07028, doi: 10.1029/2006JC003784. Thorne, P.D., P.J. Hardcastle, and R.L. Soulsby. 1993. Analysis of Acoustic Measurements of Suspended Sediments. Journal of Geophysical Research, 98, C1: 899-910. Voulgaris, G., and S.T. Meyers. 2004. Temporal Variability of Hydrodynamics, Sediment Concentration and Sediment Settling in a Tidal Creek. Continental Shelf Research, 24: 1659-1683. Vousdoukas, M.I., S. Aleksiadis, C. Grenz, and R. Verney. 2011. Comparisons of acoustic and optical sensors for suspended sediment concentration measurements under non-homogeneous solutions. Journal of Coastal Research, SI 64 (Proceedings of the 11th International Coastal Symposium): 160-164. Szczecin, Poland. Figures & Tables Figure 2: (a)110-liter calibration chamber used for the ADV inter-comparison and calibration studies (b) sensors mounted in chamber. 170 Painted Record Mean Painted Burst means Non-Painted Record Mean Non-Painted Burst means Run 2 Record Mean Run 2 Burst means 160 Acoustic Backscatter Signal (counts) Figure 1: Acoustic Attenuation Relationship to grain-size and frequency.The black box represents the sand distribution used in this study. 150 140 130 120 110 V20359 V20358 V20146 Sontek Instruments V20361 V20366 Figure 3: Nortek ADV instrument inter-comparison acoustic backscatter counts Method Detection Limit 152 Record Mean Burst means Acoustic Backscatter Signal (counts) 150 148 146 144 142 140 138 Run 1 Run 2 Run 3 Run 4 Run 5 Run 6 Nortek Run 7 Run 8 Run 9 Run 10 Figure 4: Nortek ADV method quantitation limit Method Detection Limit 132 Record Mean Burst means Acoustic Backscatter Signal (counts) 130 128 126 124 122 120 118 Run 1 Run 2 Run 3 Run 4 Run 5 Run 6 Sontek Run 7 Run 8 Run 9 Figure 5: Sontek ADV method quantitation limit Run 10 3.5 1:1 Y = 1.0666X + -0.40815 R2 = 0.98938 log10 Concentration Measured (mg/L) 3 2.5 2 1.5 July 20 July 11 All 1 1 1.5 2 2.5 log10 Concentration Added (mg/L) 3 3.5 Figure 6: log10 measured sand concentrations versus log10 expected sand concentrations 1600 1:1 Y = 0.63711X + -9.7819 1400 R2 = 0.98167 Concentration Measured (mg/L) 1200 1000 800 600 400 July 20 July 11 All 200 0 0 200 400 600 800 1000 Concentration Added (mg/L) 1200 1400 1600 Figure 7: Measured sand concentrations versus expected sand concentrations Figure 8: Nortek ADV backscatter counts versus log10 measured TSS concentrations for sand Figure 10: Nortek calibration relating acoustic backscatter counts to suspended solids concentration for sand Figure 9: Sontek ADV backscatter counts versus log10 measured TSS concentrations for sand Figure 11: Sontek calibration relating acoustic backscatter counts to suspended solid concentrations for sand