Specification:
Document filename: IM_T-Flask Adapter_dv2
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Specifications may change without prior notice.
Manufacturer
PreSens
Precision Sensing GmbH
Am BioPark 11
93053 Regensburg, Germany
Phone +49 941 94272100
Fax +49 941 94272111 info@PreSens.de
www.PreSens.de
© 2016 PreSens Precision Sensing GmbH

1 Preface .......................................................................................................................... 3
2 Description of the T-Flask Adapter ............................................................................. 4
3 Operation ...................................................................................................................... 5
3.1
Mounting the T-Flask Adapter on the SFR ................................................................. 5
3.2
Possible Adapter and Flask Positions on the SFR .................................................... 6
3.3
Positioning of the Flask on the Adapter ..................................................................... 7
3.4
Measurement ................................................................................................................ 7
4 Technical Data .............................................................................................................. 9
5 Concluding Remarks .................................................................................................. 10
© 2016 PreSens Precision Sensing GmbH

T-Flask Adapter
3
You have chosen a new, innovative technology for measuring oxygen and pH.
Chemical optical oxygen and pH sensors (also called optodes) have several important features:
They are small.
Their signal does not depend on the flow rate of the sample.
They can be physically divided from the measuring system which allows a noninvasive measurement.
They can be used in disposables.
Therefore, they are ideally suited for the examination of small sample volumes, for highly parallelized measurements in disposables, and for biotechnological applications.
A set of different oxygen and pH minisensors, flow-through cells and integrated sensor systems is available to make sure you have the sensor which matches your application.
Please feel free to contact our service team to find the best solution for your application.
Your PreSens Team
PLEASE READ THE FOLLOWING INSTRUCTIONS CAREFULLY BEFORE WORKING
WITH THIS DEVICE.
Preface
© 2016 PreSens Precision Sensing GmbH

T-Flask Adapter 4 Description of the T-Flask Adapter
The T-Flask Adapter is used for cell culture flasks with integrated sensors to conduct culture monitoring with the SFR Shake Flask Reader. It is designed to guarantee optimal positioning of the vessel and alignment above the optical module of the SFR for sensor read-out. The respective T-Flasks with integrated sensors are available in different types and sizes, and are all treated so they fit on the adapter. They come pre-calibrated and irradiated, and are readyto-use. Up to 9 T-Flask Adapter can be attached on top of the SFR. The T-Flask Adapters can also be used in combination with adapters for other cultivation vessels, e. g. clamps for shake flasks, or the iTube Adapter for cultivation tubes.
Fig. 1 T-Flask Adapter; it is attached to the SFR with
four screws and has recesses for positioning the
respective T-Flasks with integrated sensors.
T-Flask Adapter with respective T-flask equipped with oxygen and pH sensor and knobs that fit the recesses on the adapter.
Up to 9 T-Flask Adapter can be attached on top of the SFR. You need a personal computer with installed SFRS Shake Flask Reader software and installed Bluetooth adapter (SFRS and
Bluetooth adapter are delivered with the SFR Shake Flask Reader). The T-Flask Adapters can also be used in combination with adapters for other cultivation vessels, e. g. clamps for shake flasks, or the iTube Adapter for cultivation tubes.
Fig. 2 SFR equipped with different adapters to hold different
cultivation vessels; left: T-Flask Adapter
© 2016 PreSens Precision Sensing GmbH

T-Flask Adapter
5 Operation
Put the T-Flask Adapter on the SFR so the optical module is placed under the opening. Then slide the adapter into place so the adapter´s holes match the holes on the SFR (see Fig. 3).
Fig. 3 Slide the adapter into place so its holes match the
holes on the SFR
Place the four screws in the holes like shown in Fig. 4. Press down the T-Flask adapter and tighten all four screws. Make sure the adapter is attached to the SFR firmly so it does not come off during measurements.
Fig. 4 Place the four screws in the respective holes and tighten them.
© 2016 PreSens Precision Sensing GmbH

T-Flask Adapter 6
Operation
You can place up to 9 T-Flasks with integrated sensors on the SFR. The T-Flask Adapters have to be attached according to Fig. 5 with two rows facing in the same direction and one row facing in the opposite direction.
Fig. 5 T-Flask Adapter positions
© 2016 PreSens Precision Sensing GmbH

T-Flask Adapter 7
Operation
Now you can place the T-flask equipped with sensor spots and containing your media in the adapter. There are four knobs on the bottom of each T-flask with sensors and four recesses in each T-Flask Adapter to ensure the correct position of the flask and its integrated sensor spots. Put the T-flask on the adapter and slide it so the four knobs snap in the respective recesses on the adapter (see Fig. 6). As there are two bigger and two smaller knobs on the flask it is easy to determine which way the T-flask has to face, as the respective recesses on the adapter are also different in size. This way the sensors are aligned correctly for read-out with the SFR.
!
In case you want to shake the flasks, please note: The maximum shaking speed is 60 rpm!
Fig. 6 Placing the T-flask on the adapter, smaller
knobs to small recesses in the adapter
Slide the T-flask in position, so the knobs snap in the respective recesses on the adapter
Calibration
The T-flasks are delivered irradiated and pre-calibrated. Please download the calibration file(s) matching the batch number on the flask(s). You can find these files on www.presens.de/calibration . Then load the respective file(s) with the SFR software.
(Please see the SFRS instruction manual for more detailed information on calibration.)
Equilibration
!
The sensors need to be equilibrated before usage. In order to do so you have to fill the flasks with your media and wait for at least 3 hours so that the sensors can equilibrate.
© 2016 PreSens Precision Sensing GmbH

T-Flask Adapter 8 Operation
One-point adjustment pH one-point adjustment is advised if your media differs from physiological conditions.
(Please see the SFRS instruction manual for more detailed information on one point adjustment.)
Re-calibration
If you have used the pH sensor for more than 10,000 measurements, a new one-point adjustment is mandatory to ensure the accuracy of your measurements. The oxygen sensor requires no re-calibration up to 100,000 measurement points.
Do not use the T-flasks with integrated sensors for more than 100,000 measurement points.
Operational notes during measurement
The SFRS is for controlling your measurement. It offers different options to display and monitor your measurement data:
1. Virtual tray: showing the DO and pH values of all 9 positions
2. 9 positions graph: showing the graph of DO and pH of all 9 positions
3. Detailed graph: detailed graph of one position
4. Cumulated graph: for overlaying different graphs of the positions
First you should check whether an error or “no sensor detected” message is displayed for pH or DO measurement (see Fig. 7). If this is the case, the flasks might not have snapped in properly.
Fig. 7 Error message appears, if the flask is
not locked in place properly
No error message appears, if the flask is locked in place properly.
Please check, if you have placed the T-Flask Adapters and/or flasks in the right way and adjust their positioning if necessary. Check the signal quality of the sensors again. If the problem persists, please contact our service team.
© 2016 PreSens Precision Sensing GmbH

T-Flask Adapter 9
Technical Data
Sensor Specifications
Measuring range
Oxygen
0 - 100 % O
Response time (t
90
) at 25 °C < 60 s
2
Resolution
Accuracy
Drift
Properties
Temperature range
Compatibility pH*
5.5 - 8.0 pH
± 0.01 % O
2
at 0.21 % O
2
± 0.1 % O
2
at 20.9 % O
2
± 0.4 % O
2
at 20.9 % O
2
< 60 s
± 0.01 pH at pH = 7**
± 0.05 % O
2
at 0.2 % O
2
± 0.1 pH at pH = 7 with one-point adjustment
± 0.2 pH at pH = 7 with precalibration
< 0.01 % O
2
per day
(sampling interval of 1 min.)
< 0.01 pH per day
(sampling interval of 1 min.) from 5 °C to 50 °C
Cross-sensitivity
Storage stability aqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10 typically no cross-sensitivity in culture media reduced to ionic strength (salinity); a high concentration of small fluorescent molecules in the visible range can interfere
12 months provided the sensor is stored in the dark
Shake flasks with integrated sensors are delivered irradiated.
* provided shake flasks with integrated sensors are used without further handling in physiological
solutions
** at 100 rpm & cell culture media
© 2016 PreSens Precision Sensing GmbH

T-Flask Adapter 10
Concluding Remarks
Dear Customer,
With this manual, we hope to provide you with an introduction to work with the T-Flask
Adapter.
This manual does not claim to be complete. We are endeavored to improve and supplement this version.
We are looking forward to your critical review and to any suggestions you may have.
You can find the latest version at www.PreSens.de.
With best regards,
Your PreSens Team
© 2016 PreSens Precision Sensing GmbH

Manufacturer
PreSens
Precision Sensing GmbH
Am BioPark 11
93053 Regensburg
Germany
Phone +49 941 94272100
Fax +49 941 94272111 info@PreSens.de www.PreSens.de
© 2016 PreSens Precision Sensing GmbH