CAREER HISTORY OF RAFAEL RUBIO
Curriculum Vitae RAFAEL RUBI0
Place of birth: Queretaro, Qro. Mexico. 1928
Academic training
Mexico Science 1963
Syntex S.A. Mexico
Inst. Nac. Cardiol. Mex.
Western Reserve Univ.
Univ. de Virginia
Univ. de Virginia
Univ. de Virginia
Univ. de Virginia
Univ. de Virginia
Univ. de Virginia
Univ. Aut. San Luis Pot.
Occupied Positions :
Research Division Research Asóciate
Physiology Dept. Technical Reearch
Physiology Dept.
Physiology Dept.
Physiology Dept.
Physiology Dept.
Research Associate
Postdoctoral Fellow
Asistant Professor
Associate Professor
Physiology Dept.
Physiology Dept.
Professor
Emeritus Professor
Cardiovasc.Center Visiting Res.Scholar
Depto. Fisiologia Profesor VI
Honors:
Fogarty International Fellow 1983. Who is Who in America (Since1983)
Who’s Who in Science (Since 1975)
1952-1955
1955-1963
1964-1966
1968-1969
1969-1972
1972-1977
1977-1995
1995-presente
1996-presente
1996- presente
Investigador Nacional: Sistema Nacional de Investigadores NIVEL III. (Since 1996 hasta 2013)
Awards :
Univ. of Va. “Inventor of the year; 1993”, $ 20,000 Dlls
Johnson & Johnson “Setting New Directions in Science and Technology” 1991. $ 450,000 Dlls
“OHTLI 1996” (Sec. Rel.Ext. Mex) “For Distinguíshed Services to the Mexican Community Abroad” Medal
Member of Editorial Boards:
Amer J. Physiol (1972-1986), Circ. Res (1975-1990), y Can. J. Physiol. Pharmacol.
Member of Study and Evaluation committees:
National Institute of Aging Nat. Inst. Health 1979-1982. Amer. Heart Assoc., Va. Chapter (1976-1980)
National Science Foundation 1991- 2000. National Research council. 1991 – 1998.
Oak ridge Associated Universities 1994- 1999. Amer. Heart Assoc. Scientific Council., CONACYT; Comite de Ciencias Naturales 1999-2003, CONACYT; Comite de Campos Emergentes 1999-2003, CONACYT;
Comite evaluacion del Sistema Nac. De Investigadores 2006-2009
Scientific Societies :
American Physiological Society, Circulation Group de la Amer. Physiol Soc., American Heart Association;
Scient. Council, Sociedad Mex. de Ciencias Fisiologicas, Sociedad Mexicana de Cardiologia,
Sociedad Mexicana de Bioquimica, North American Vascular Biology Organization.
Internationa Soc. for Heart Researh. American Society of Matrix Biology
Patents:
UNITED STATES PATENTS .
No. 4,673,563. “ADENOSINE IN THE TREATMENT OF SUPRAVENTRICULAR TACHYCARDIA”.
Inventors: Robert M. Berne, Luis Belardinalli and Rafael Rubio
No. 4,364,922. “ADENOSINE ANTAGONISTS IN THE TREATMENT AND DIAGNOSIS OF A-V NODE
CONDUCTION DISTURBANCES”. Inventors: Robert M. Berne, Luis Belardinelli and Rafael Rubio
No. 5,424, 297. “ADENOSINE DEXTRAN CONJUGATES”. Inventors: Rafael Rubio, Eduardo Balcells and
Jorge Suarez
Invention disclosure RR02 Univ. of Virginia. “NITRIC OXIDE AND NITRIC OXIDE-PRODUCING AGENTS

IN THE TREATMENT AND DIAGNOSIS OF SUPRAVENTRICULAR TACHYCARDIA”. Inventors: Rafael
Rubio, Guillermo Ceballos and Eduardo Balcells.
MEXICAN PATENTS .
Patent proposal (In process of submission 2010). “ I.
To selectively and exclusively block luminal membrane endothelial AT
1
receptors with Angiotensin II receptor blockers (ARB) confined to the vascular lumen. II. To selectively and exclusively inhibit luminal membrane endothelial Angiotensin
Convertin Enzyme (ACE) with ACE inhibitors confined to the vascular lumen. III . An innovative approach for the cure and prevention of Ang II-dependent cardiovascular diseases. Inventors: Dr.
Rafael Rubio, MC. Jose David Torres Tirado, QFB. Erika Chi Ahumada, Dr. Juan Manuel Ramiro
Díaz, QFB. Rebeca Ramos Flores
Mentors and Trainees
Mentors; 3 Postdoctoral fellows; 24 Grad Stud; Doctors; 20 Grad Stud; Masters; 8
MENTORS:
1. Walter B. Cannon, 2. Arturo Rosenblueth
3. Robert M. Berne.
TRAINEES (Name, Dates, Country, Role)
1. Schrader Jurgen (1971-1973, Germany,
Posdoc)
2. Wiedmeier Thomas (1971-1973, USA, Posdoc)
3. Mustafa Jamal (1971-1975, India, Posdoc)
4. Bockman Emma (1972-1975, USA, Posdoc)
5. Degenring Fritz (1974-1976, Germany, Posdoc)
6. Thomas Rosemary (1969-1974,USA,Grad.
Stud)
7. Mentzer Robert (1975-1976, USA, Surg.
Resident)
8. Winn Richard (1973-1979, USA, Surg.
Resident)
9. Belardinelli Luis (1977-1979, Brasil, Posdoc)
10. Miller Wayne (1973-1977, USA, Posdoc)
11. Watkinson Penn (1973-1978, USA, Grad.
Student)
12. Thompson Carl (1976-1980, USA, Grad.
Student)
13. Foley Duane (1977-1978)
14. Bacchus Alvan (1979-1982, Antilles, Posdoc)
15. Fenton Richard (1978-1982, USA, Posdoc)
16. Tsukada Tokumasu (1980-1982, Japan,
Posdoc)
17. Ely Stephen (1982-1984, USA, Posdoc)
18. Belloni Frank (1979-1982, USA, Posdoc)
19. Knabb Robert (1978-1982, USA Grad
Student)
20. Knabb Maureen (1978-1983, USA, Grad.
Student)
21. Kumwatana Permsak (1978-1983,
Thailand,Grad. Student)
22. Malloy Laura (1980-1985, USA, Posdoc),
23. Bencherif Merouane (1980-1987, Algiers,
Grad. Student)
24. Gidday Jeffrey (1982-1987,USA, Grad.
Student)
25. Shryock John (1985-1988, USA, Posdoc)
26. Meghji Parviz (1987-1989, England, Posdoc)
27. Magdalena Torres (1991-1993, Spain,
Posdoc)
28. Balcells Eduardo (1991-1993, USA, Med.
Student)
MEXICO ( Antes de 1996)
29 . Gustavo Zubieta (1961, Mexico, Posdoc)
30. Gustavo Pastelin (1962, Mexico St)
31. Suarez Jorge (1989-1992, Mexico, Posdoc)
32. Ceballos Guillermo (1991-1994, Mexico,
Posdoc)
MEXICO (1996 until present )
33. Zenteno Tania (1997-1999, Mexico, Posdoc)
34. Katz Michael (1997, USA, Med. Student)
35. Gonzalez Carmen (1997-2000, Mexico, Grad.
Student)
36. Lio Francisco (1998, USA, Undergrad.
Student)
37. Sada Isabel (1999-2001, Mexico, Grad.
Student)
38. Alvergue Juan (1999, USA, Undergrad.
Student)
39. Rubio-Gayosso Ivan (1999-2003, Mexico,
Grad. Student)
40. Castillo Gonzalez Jesus (2000-2005, Mexico,
Grad. Student)
41. Ramiro Juan (2004-2005, Mexico, Grad.
Student)
42. Barajas Espinosa Alma (2003-2006, Mexico,
Grad. Student)
43. Sandate Nancy (2006, Mexico Undergrad.
Student)
44. Arroyo Blanca (2005-2008, Mexico, PostDoc)
45. David Torres (2007-, Mexico, Grad St)
46. Sandra Perez (2007-, Mexico, Grad St)
47. Maureen Knabb (2009-2010, USA Visiting
Professor)

1. American Heart Association, Grant # HL-74962 .
Princ. Investigator; Rafael Rubio
1, –
2. National Institutes of Health, Grant # HL-10384
R. M. Berne and Rafael Rubio; Co-investigators.
1, –
Sept 1, 1978 - Aug 31, 1983
Sept 1, 1983 - Aug 31, 1988
2. National Institutes of Health, Grant # 19242
Vascular Smooth Muscle Program Project.
Sub-project 3; Princ. Investigator Rafael Rubio
1, –
$ 706,000
$ 900,000
1, –
3. National Institutes of Health ,
Fogarty Grant # FOGTW00821, Award to Rafael Rubio
9, – 32,615
4. National Institutes of Health, Grant # HL-19242
Vascular Smooth Muscle Program Project.
Sub-project 3; Princ. Investigator Rafael Rubio
1, –
5. Johnson & Johnson; Focusing Giving Program Award
“Setting New Direction in Science & Technology”
Award to Rafael Rubio
Jan 1, 1989 – Dec 31, 1991
6. National Science Foundation Grant # IBN-9120353
Princ. Investigator Rafael Rubio
1, –
7. National Institutes of Health, Grant # HL-47367
Princ. Investigator Rafael Rubio
1, –
$ 450,000
USA $1,332,127
TOTAL COST; USA Dlls $5,138,203

:
1.UNIVERSITY OF VIRGINIA.
Donation to Rafael Rubio
Equipment, materials, reagent donation.Sept 1996
2. CONACYT. Repatriacion de R. Rubio y equipo. Sept 1996
3. FUNSALUD . Repatriacion de R. Rubio. Sept 1995
4. CONACYT 0433P . Invest. Princ Rafael Rubio
Sept 1995 – Agos 1998
UASLP . Fondos Concurrentes .
5. CONACYT 25963-N. Invest Princ. Rafael Rubio.
Sept 1998 – Agos 2001
UASLP . Fondos Concurrentes .
6. CONACYT G34998-N.
Invest Princ. Rafael Rubio
Abr –
UASLP . Fondos Concurrentes .
SHIGO 20000202018 . Invest Princ.. Rafael Rubio
Agos –
CONACYT Ref. SEP-2003-CO2-42567 . Invest Princ.
Agos 2004 – Sept 2008
UASLP . Fondos Concurrentes
CONACYT. Salud-2004-C01-156.
Oct 2005 - Nov 2008.
CONACYT Ref. SEP-CB-2008-101850 . Invest Princ.
Abril Mayo
UASLP . Fondos Concurrentes
$ 3,500,000
$ 500,000
$ 100,000
$ 600,000
$ 765,286
$
$ 1,328,512
$ 2,000, 000

INVITED LECTURES. TOTAL 40
1. Symposium during 2010 Annual congress of Soc. Mex. Cienc. Fisiol. Villahermosa Tabasco
“Diverse Intrinsic Regulatory Mechanisms of Cardiac Contraction: One of Starling’s Legacies”
Organizer, Chair and Speaker.
2. Symposium during the 2010 annual Fed. Amer. Soc. Exper. Biol. meeting. Anaheim. Cal USA
“One Hundred Years of Starling: His Contributions to Physiology” Organizer and Chair
3. Symposium during the 2009 annual meeting of Asoc. Mex. Preven. Arteriosc y sus Complic.
(AMPAC). “Isquemia y reperfusion coronaria: del Laboratorio básico a la unidad coronaria”.
Speaker
4. Symposium during the 2nd congreso Innovacion en Cardiologia y medicina. Inst. Cardiol y Med.
Vasc. “Metabolismo” Speaker
5. 1er Congreso Nacional “Union de la Ciencia Basica y la Clinica” ANCISSSTE/AMPAC. 2007.
Lecture: “SUPERFICIE ENDOTELIAL LUMINAL EN ISQUEMIA-REPERFUSION: ALTERACIONES Y POSIBLES
MECANISMOS”.
6. 1er Congreso Nacional “Union de la Ciencia Basica y la Clinica” ANCISSSTE/AMPAC. 2007.
Lecture: “ADENOSINA Y FLUJO CORONARIO Y FUNCIONES PARACRINAS DE LA ADENOSINA”.
7. Symposium during 2007 Annual congress of Soc. Mex. Cienc. Fisiol. Puebla, Puebla.
“Functionality of the luminal membrane of vacular endothelium”. Organizer, Chair and Speaker.
8. Symposium during 2007 Annual congress of Soc. Mex. Cienc. Fisiol. Puebla, Puebla.
“Science, Education and Advocacy”. Speaker
9. Course “Glycobiology: an introduction to the biological functions of glycoproteins and glycolipids”
Organizer and participant. UASLP 2006
10. Symposium during the 2004 annual congress of the Internat. Soc. Heart Research. “Coronary
Circulation and Ischemia”. Organizer, Chair and Speaker.
11. Congreso Soc. Pot. De Cardiologia 1999. Lecture “Activation selectiva y exclusiva de receptores intravasculares del endotelio coronario para adenosina y acetilcolina”
12. 1997 Annual Congress of Australian and New Zeland Cardiological Soc. Australia. Lecture
“Role of coronary endotelial luminal adenosine receptors”
13. 1997 Annual Congress of Australian and New Zeland Cardiological Soc. Australia. Lecture
“The importance of coronary flow as regulator of cardiac function: Molecular mechanisms.
14. Lecture “El flujo coronario: una senal regulada por y reguladora de la función cardiaca” Univ.
Zacatecas, Zac. 1997.
15. Symposium during 1994 annual congress Soc. Mex. Cardiologia. “Funtionality of endothelium in health and diseased states: A comprehensive review”. Organizer and lecturer.
16. 1990 Annual congress Sociedad mexicana de Cardiologia. Morelia Mich. Lecture “Functional
Interactions Between endotelial-Parechymal cells”
17. 1992. Lecture “Functional significance of coronary flow as a regulatory signal: Possible molecular mechanisms” Dept. Physiology, Jefferson Univ. Philadelphia Penn.
18. 1987 Annual Congress Soc. Mex. Cienc. Fisiol. Symposium “Musculos esqueletico y cardiaco”
Lecture. “Significado de la produccion de adenosina en corazon”.
19. 27 th
Annual Richard Symposium Pennsylvania 1986. Lecture “Adenosine: site of release in the nervous system and control of phosphatidylinositol turnover”
20. 3 rd
International symposium on adenosine 1986. Lecture “A critique on the use of adenosine deaminase to test the adenosine hypothesis: Disregarded implicit assumptions”.
21. 2 nd
International symposium on adenosine 1982. Organizer and Lecturer “Mechanisms of action of adenosine on vacular smooth muscleand cardiac muscle”.
22. 1 st
International symposium on adenosine 1979. Lecture “Cardiac adenosine:
Electrophysiological effects, possilbe significance in cell function and mechanisms controlling its release”.
23. 7 th
Internat. Symp. on Cerebral Blood Flow and Metabolism, 1975. Sclotand UK. Lecture
“Relationship of Adenosine Concentation, Lactate Levels and Oxygen Supply in Rat Brain”

24. Lecture “Role of Adenosine in regulation of coronary and cerebral blood flow; molecular mechanisms of adenosine production”. Dept Physiology, Duke Univ.
25. Ciba Foundation Symposium 56, 1973. Lecture “Production, metabolism and possible functions of adenosine in brain tissue in situ.
APPROXIMATELY ANOTHER 15 INVITED LECTURES ARE NOT LISTED.
ABSTRACTS SUBMISSION TO INTERNATIONAL AND NATIONAL CONGRESS: TOTAL 110
On the average two abstracts per year are submitted.
List of Publications (total 190 publications. C itations on Citation Index > 10,000)
1. Rosenblueth, A. and R. Rubio. La influencia de la frequencia de estimulacion sobre los tiempos de propagacion auriculo-ventricular. Arch. Inst. Nac. Cardiol. Mex. 25:535-570, 1955.
2. Rubio, R. and A. Rosenblueth. La organizacion funcional de los tejidos ventriculares.Arch. Inst.
Nac. Cardiol.Mex. 26: 644-663, 1956.
3. Rosenblueth, A., J. Alanis, and R. Rubio. A comparative study or the isotonic and isometric contractions of the striated muscles. Archiv. Internat. Physiol. Biochem. 66: 330-353, 1958.
4. Rosenblueth, A., J. Alanis, and R. Rubio. The influence of initial load and length on the amplitud of muscular contractions. Arch. Internat. Physiol. Biochem. 66: 354-372, 1953.
5. Rubio, R.; and A. Tohen. Las curvas de intensidad duracion en la clinica. Acta Neurol.
Latinoamer. 5: 195-112, 1959.
6. Rubio, R.,and L. Sierra. Fisiologia de las transposiciones musculares. Ann. Rehabilit. 5: 195-
207, 1959.
7. Rubio, R. Las contraciones isotonicas de los musculos estriados. Acta Physiol. Latinoamer. 9:
298-305, 1959.
8. Rubio, R. La distribucion de la tension en el musculo durante el reposo y la contraccion . Acta
Physiol. Latinoamer. 9: 306-312, 1959.
9. Rosenblueth, A., J. Alanis, and R. Rubio. Some properties of the mammalian ventricular muscle.
Arch. Internat. Physiol. Biochem. 67: 276-293, 1959.
10. Rosenblueth, A., J. alanis, E. Lopez, and R. Rubio. The adaptation of the ventricular muscle to different circulatory conditions. Arch. Internat. Physiol. Biochem. 67: 358-373, 1959.
11. Rosenblueth, A., J. Alanis, R. Rubio, and E. Lopez. The two staircase phenomena. Arcn.
Internat. Physiol. Biochem. 67: 374-383, 1959.
12. Rudomin, P. and R. Rubio. Actividad subliminal de los quimioreflejos originados por el cianuro de potasio. Acta Physiol. Latinoamer. 9: 194-200, 1959.
13. Rosenblueth, A and R. Rubio. The time-course of the isometric and the isotonic twitches of striated muscles. Arch. Internat. Physiol. Biochem. 67: 718-731, 1959.
14. Rosenblueth, A., and R. Rubio. The velocity of shortening of striated muscles. Arch. Internat.
Physiol. Biochem. 67: 705-717, 1959.
15. Rosenblueth, A., and R. Rubio. Tetanic summation and isometric responses. Arch. Internat.
Physiol. Biochem. 68: 165-180, 1960.
16. Rosenblueth, A., and R. Rubio. The relations between isometric and isotonic contractions.
Arch. Internat. Physiol. Biochem. 68: 181 -189, 1960.
17. Rosenblueth, A., J. Alanis, R. Rubio and G. Pilar. Relations between coronary flow and the work of the heart. Am. J. Physiol. 200: 243-246, 1961.
18. Rubio, R, and G. Zubieta. The variations of the electric resistance of the microelectrode during the flow of current. Acta Physiol. Latinoamer. 11: 91 -94, 1961
19. Rubio, R. and G. Pastelin. Las repuestas repetitivas de la fibra muscular estriada. I. Los dos comportamientos de la fibra muscular estriada. Acta Physiol. Latinoamer. 13: 139-149, 1963.
20. Rubio, R. Las repuestas repetitivas de la fibra muscular estriada. Il. El efecto de la estimulacion repetida con microelectrodos de KCL and NaCI sobre la amplitud de las respuestas.
Acta Physiol. Latinoamer. 14: 99-107,1964.
21. Rubio, R. La autoregulacion de los potenciales propagados. Acta Physiol. Latinoamer. 13: 260-
265, 1963.
22. Rubio, R. Los cambios de resistencia de membrana provocados por la posicion del microelectrodo. Acta Physiol. Latinoamer. 13: 266-268, 1963.
23. Alanis, J., R. Rubio, and D. Masher. Metodo que resuelve algunos problemas en el estudio de la Circulacion Coronaria. Arch. Inst. Nac. Cardiol. Mex. 33: 157-164, 1963.

24. Herzberg, R., R. Rubio, and R. Berne. Coronary occlusion and embolization: effect on blood flow in adjacent arteries. Amer. J. Physiol. 210: 169-175, 1966.
25. Rubio, R., R. M. Berne, and M. Katori. Release of adenosine in reactive hyperemia of the dog heart. Am. J. Physiol. 216: 55-62, 1969.
26. Rubio, R., and R. M. Berne. The release of adenosine by the normal myocardium and its relationship to the regulation of coronary resistance. Circulation Res. 25: 407-415, 1969.
27. Berne, R. M. and R. Rubio. Acute coronary occlusion: early changes that induces coronary dilation and the development of collateral circulation. Amer. J. Cardiol. 24: 776-781, 1969.
28. Berne, R. M., and R. Rubio. Factors influencing coronary resistance in the ischemic heart.
Circulation. 39; (IV); 240-250, 1969.
29. Sperelakis, N., R. Rubio, and J. Redick. Sharp discontinuity in sarcomere length across intercalated discs of fibrillating cat hearts. J. Ultrastruct. Res. 30: 503-532, 1970.
30. Berne, R M., R. Rubio, B.R. Duling, and V.T. Wiedmeier. Effects of acute and chronic hypoxia on coronary blood flow. Adv. Cardiol. 5: 56-66, 1970.
31. Rubio, R., and N. Sperelakis. Entrance of colloidal ThO2 tracer into the T tubules and longitudinal tubules of guinea pig hearts. Z. Zellforsch. 116: 20-36, 1971.
32. Berne, R.M., R. Rubio, J.G. Dobson, and R.R. Curnish. Adenosine and adenine nucleotides a.s possible mediators of cardiac and skeletal muscle blood flow regulation . Circulation Res. 28:
115-119, 1971.
33. Sperelakis, N. and R. Rubio. An orderly lattice of axial tubules which interconnect adjacent transverse tubules in guinea-pig ventricular myocardium. J. Mol. & Cell. Cardiol. 2: 212-220, 1971.
34. Sperelakis, N., and R. Rubio. Ultrastructural changes produced by hypertonicity in cat cardiac muscle. J. Mol. & Cell. Cardiol. 3: 139-156, 1971.
35. Dobson, J.G., Jr., R Rubio, and R.M. Berne. Role of adenine-nucleotides, adenosine and inorganic phosphate in regulation of skeletal muscle blood-flow. Circulation Res. 29: 375-384,
1971.
36. Rubio, R., and N. Sperelakis. Penetration of horseradish peroxidase into the terminal cisternae of frog skeletal muscle fibers and blockade of caffeine contracture by Ca+~ depletion. Z.
Zellforsch. 124: 57-71, 1972.
37. Rubio, R., V.T. Wiedmeier, and R.M. Berne. Nucleoside phosphorylase: Localization and role in the myocardial distribution of purines. Am. J. Physiol. 222: 550-555, 1972.
38. Wiedmeier, V.T., R. Rubio, and R.M. Berne. Incorporation and turnover of U-14C-Adenosine in perfused guinea pig myocardium. Am. J. Physiol. 2: 51-54, 1972.
39. Schrader, J., R.M. Berne, and R. Rubio. Uptake and metabolism of adenosine by human erythrocyte ghost cells. Amer. J. Physiol. 223: 159-166, 1972.
40. Forbes, M.S., R. Rubio, and N. Sperelakis. Tubular systems of limulus myocardial cells investigated by use of electron-opaque tracers and hypertonicity. J. Ultrastructure Res. 39: 580-
597, 1972.
41. Mustafa, S.J., R. Rubio, and R.M. Berne. Effect of detergents and homogenization on adenosine kinase and adenosine deaminase of human erythrocyte ghosts. Proc. Soc. Expt'l. Biol.
Med. 141: 161-163, 1972.
42. Curnish, R.R., R.M. Berne, and R. Rubio. Effect of aminophylline on myocardial reactive hyperemia. Proc. Soc. Expt'l Biol. Med. 141: 593-598, 1972.
43. Wiedmeier, V.T., R. Rubio, and R.M. Berne. Inosine incorporation into myocardial nucleotides.
J. Moll. and Cell. Cardiol. 4: 445-452, 1972.
44. Bockman, E.L., R. Rubio, and R.M. Berne. Effect of lanthanum on isoproterenol induced activation of myocardial phosphorylase. Am. J. Physiol. 225: 438-443, 1973.
45. Sperelakis, N., R. Valle, C. Orozco, A. Martinez-Palomo, and R. Rubio. Electromechanical uncoupling of frog skeletal muscle by possible change in sarcoplasmic reticulum content. Am. J.
Physiol. 225: 793-800, 1973.
46. Rubio, R., R.M. Berne, and J.G. Dobson, Jr. Sites of adenosine production in cardiac and skeletal muscle. Am. J. Physiol. 225: 938-953, 1973.
47. Berne, R.M. and R. Rubio. Challenges to the adenosine hypothesis for the regulation of coronary blood flow. In: Current Topics in Coronary Research. Ed. C.M. Bloor and R.A. Olsson.
Plenum Publishing Co., N.Y.1973, pp. 3-10.
48. Berne, R.M. and R. Rubio. Adenine nucleotide metabolism in the heart. Circulation Res. Suppl.
35: 111-109-111-120, 1974.

49. Sperelakis, N., M.S. Forbes, and R. Rubio. The tubular systems of myocardial cells:
Ultrastructure and possible function. In: Myocardial Biology. Vol. 4, pp 163, edited by N.S. Dhalla.
University Park Press, 1974.
50. Berne, R.M., R. Rubio, and R.R. Curnish. Release of adenosine from ischemic brain, its effects on cerebral vascular resistance and its incorporation into cerebral adenine nucleotides. Circulation
Res. 35: 262-271, 1974.
51. Berne, R.M. and R. Rubio. Regulation of coronary blood flow. Adv. Cardiology 12: 303-312,
1974.
52. Rubio, R. Purine nucleoside phosphorylase. In: Electron Microscopy of Enzymes: Principles and Methods. Ed. M.A. Hayat. Van Nostrand Reinhold Co., N.Y. 1974.
53. Berne, R.M. and R. Rubio. Adenine nucleotide metabolism in the heart. Circulation Res.
(Suppl. Ill) 34 and 35: 109-120, 1974.
54. Rubio, R., V.T. Wiedmeier, and R.M. Berne. Relationship between coronary flow and adenosine production and release. J. Mol. Cell. Cardiol. 6: 561-566, 1974.
55. Mustafa, S.J., R. Rubio, and R.M. Berne. Uptake of adenosine by dispersed chick-embryo heart cells. Am. J. Physiol. 228: 62-67, 1975.
56. Bockman, E.L., R.M. Berne and R. Rubio. Release of adenosine and lack of release of ATP from contracting skeletal muscle. Pflugers Arch. 355: 229-241, 1975.
57. Schrader, J., R. Rubio, and R.M. Berne. Inhibition of slow action potentials of guinea pig atrial muscle by adenosine: A possible effect on Ca++ influx. J. Molec. Cell. Cardiol. 7: 427-433, 1975.
58. Thomas, R.A., R. Rubio, and R.M. Berne. Comparison of the adenine nucleotide metabolism of dog atrial and ventricular myocardium. J. Molec. Cell Cardiol. 7: 115-123, 1975.
59. Mustafa, S.J., R.M. Berne and R. Rubio. Adenosine metabolism in cultured chick-embryo heart cells. Am. J. Physiol. 228: 1474-1478, 1975.
60. Degenring, F., R. Rubio, and R.M. Berne. Adenine nucleotide metabolism during cardiac hypertrophy and ischemia in rats. J. Molec. Cell. Cardiol. 7: 105-113, 1975.
61. Rubio, R., R.M Berne, E.L. Bockman, and R.R. Curnish. Relationship between adenosine concentration and oxygen supply in rat brain. Am. J. Physiol. 228: 1896-1902, 1975.
62. Rubio, R. and R.M. Berne. Relationship of adenosine concentration, lactate levels and oxygen supply in the rat brain. In Blood flow and Metabolism in the Brain. Ed. M. Harper, B. Jennett, D.
Miller and J. Rowan. Churchill Livingstone, New York, 1975, pp. 3.23-3.27.
63. Rubio, R. and C. Villar-Palasi. Effects of c-AMP dependent protein kinase on cardiac actomyosin: Increase in Ca++ sensitivity and possible phosphorylation of troponin I . J. Cyclic
Nuct. Res. 1: 143-150, 1975.
64. Rubio, R. and R.M. Berne. Regulation of coronary blood-flow. Prog. Cardiovasc. Dis. 18: 105 -
117, 1975.
65. Mentzer, R.M., R. Rubio and R.M. Berne. Release of adenosine by hypoxic canine lung tissue and its possible role in pulmonary circulation. Am. J. Physiol. 229: 1625-1631, 1975.
66. Bockman, E.L., R.M. Berne, and R. Rubio. Adenosine and active hyperemia in dog skeletal muscle. Am. J. Physiol. 230: 1531 -1537, 1976.
67. Herlihy, J.T., E.L. Bockman, R.M. Berne, and R. Rubio. Adenosine relaxation of isolated vascular smooth muscle. Am. J. Physiol. 230: 1239-1243, 1976.
68. Rubio, R. and R.M. Berne. Intrinsic factors in the control of cerebral circulation. Current
Concepts of Cerebrovascular Disease-Stroke. 11: 21 -25, 1976.
69. Degenring, F.H., R.R. Curnish, R. Rubio and R.M. Berne. Effect of dipyridamole on myocardial adenosine metabolism and coronary flow in hypoxia and reactive hyperemia in the perfused guinea pig heart J. Mol. Cell. Cardiol. 8: 877-888, 1976.
70. Berne, R.M., J.T. Herlihy, J. Schrader and R.Rubio. Effect of adenosine on contraction of vascular smooth muscle. In "lonic actions on vascular smooth muscle." Ed. E. Betz. Springer-
Verlag, Berlin, Germany, 1976.
71. Rubio, R. and R.M. Berne. Myocardial circulation. In the Peripheral Circulation. Ed. P.C.
Johnson. John Wiley and Sons. N.Y., 1977.
72. Berne, R.M. and R. Rubio. Circulatory effects of tissue oxygen tension sensors. In: Tissue
Hypoxia and Ischemia. Advances in Experimental Biology & Medicine. Eds. M. Reivich, R. Coburn,
S. Lahiri and B. Chance. 78: 163-174, 1977.
73. Berne, R.M., and R. Rubio. Control of the myocardial microcirculation. In: Oxygen &
Physiological Function, Ed. F.F. Jobsis, Professional Information Library, Dallas, Texas, 1977, pp.
403-417.

74. Van Harn, G.L., R. Rubio, and R.M. Berne. Formation of adenosine nucleotide derivatives in isolated hog carotid artery strips. Am. J. Physiol. 233(2): H299-H304, 1977.
75. Berne, R.M., and R. Rubio. Common Denominators in the Regulation of Blood Flow in Heart and Brain. In: Brain and Heart Infarct. Eds. Zulch, Kaufmann, Hossmann, and Hossman. Springer-
Verlag, Berlin, 1977, p. 1929-1938.
76. Sperelakis, N., K. Shigenobu, and R. Rubio. 3H-sucrose compartments in frog skeletal muscle relative to sarcoplasmic reticulum. Am. J. Physiol. 234: C181-C190, 1978.
77. Foley, D.H., H.T. Herlihy, C.l. Thompson, R. Rubio, and R.M. Berne. Increased adenosine formation by rat myocardium with acute aortic constriction. J. Molec. Cell. Cardiol. 10: 293-300,
1978.
78. Rubio, R., R.M. Berne, and H.R. Winn. Production, metabolism and possible functions of adenosine in brain tissue in situ. In Cerebral Vascular Smooth Muscle and its Control. Ed. Purves
M.J. Ciba Foundation Symposium 56, 1973, Elsevier/Excerpta Medica/North Holland. pp 355-372.
79. Berne, R.M. and R. Rubio. Role of adenosine, adenosine triphosphate and inorganic phosphate in resistance vessel vasodilatation. In: Mechanisms of Vasodilatation. Eds. P.M.
Vanhoutte and 1. Leusen. S. Karger, Basel, 1978, pp. 214-221.
80. Berne, R.M. and R. Rubio. Regional metabolic regulation of myocardial blood flow and possible alterations leading to abnormal vasoconstriction. In: Primary and Secondary Angina Pectoris. Eds.
A. Maseri, G.A. Klassen and M. Lesch. Grune & Stratton, 1978, pp. 211 -216.
81. Miller, W.L., R.A. Thomas, R.M. Berne, and R. Rubio. Adenosine production by the ischemic kidney. Circ. Res. 43: 390-397. 1978
82. Rubio, R., L. Belardinelli, C.l. Thompson, and R.M. Berne. Cardiac adenosine:
Electrophysiological effects, possible significance in cell function, and mechanisms controlling its release. In Physiological and regulatory functions of adenosine and adenine nucleotides. H.P. Baer and G. Drummond, eds. Raven Press 1979, pp. 167-182.
83. Berne, R.M., D.H. Foley, W.P. Watkinson, W.L. Miller, H.R. Winn, and R. Rubio. Role of adenosine as a mediator of metabolic vasodilation in the heart and brain a brief overview and update. In Physiological and regulatory functions of adenosine and adenine nucleotides. H.P. Baer and G. Drummond, eds. Raven Press, 1979, pp. 117-126.
84. Watkinson, W.P., D.H. Foley, R. Rubio, and R.M. Berne. Myocardial adenosine formation with increased cardiac performance in the dog. Am. J. Physiol. 236: H13-H21, 1979.
85. Harder, D.R., L. Belardinelli, N. Sperelakis, R. Rubio, and R.M. Berne. Differential effects of adenosine and nitroglycerin on the action potentials of large and small coronary arteries. Circ. Res.
44:176-182, 1979.
86. Belardinelli, L., R. Rubio, and R.M. Berne. Blockade of Ca dependent rat atrial slow action potentials by adenosine and lanthanum. Pflugers Archiv., 380: 19-27, 1979.
87. Belardinelli, L., D. Harder, N. Sperelakis, R. Rubio and R.M. Berne. Cardiac glycoside stimulation of inward Ca++ current in vascular smooth muscle of canine coronary artery. J.
Pharmacol. Exper. Therap. 209: 62-66,1979.
88. Berne, R. M. and R. Rubio. Coronary Circulation. IN: Handbook of Physiology, Section 2. The
Cardiovascular System. Volume 1, (The Heart) American Physiological Soc., Bethesda, Md. 1979, pp. 873-952.
89. Foley, D.H., W.L. Miller, R. Rubio, R. M. Berne. Transmural distribution of myocardial adenosine content during coronary constriction. Am. J. Physiol. 236: H832-H838, 1979.
90. Winn, H. R.,C.Bryner, R. R. Curnish, R. R. Rubio, & R. M. Berne. Changes in Brain Metabolites
During the First 60 seconds of Ischemia: Adenosine Production during Hypotension. Trans. Amer.
Neur. Assoc. Vol. 103, p. 1-5, 1979.
91. Berne, R. M., H.R. Winn, T.S. Park, J.E. Welsh, A.B. Bowe, R.R. Curnish, & R. Rubio.
Mechanism of adenosine incorporation into brain. Proceedings of the IXth International
Symposium on Cerebral Blood Flow & Metabolism. Eds. F. Gotoh, H. Nagai and Y. Tazaki. Japan,
1979, pp 60-61.
92. Winn, H. R., A.B. Bowe, J.E. Welsh, R. Rubio and R.M. Berne. Cerebral adenosine levels after acute and sustained alteration in cerebral perfusion pressure. Proceedings of the IXth International
Symposium on Cerebral Blood Flow & Metabolism. Eds. F. Gotoh, H. Nagai and Y Tazaki.
Hidejima Seihanjo, Japan, 1979, pp. 328-329.
93. Winn, H. R., A.B. Bowe, J.E. Welsh, R. Rubio and R.M. Berne. Changes in adenosine during sustained hypoxia. Proceedings of the IXth International Symposium on Cerebral Blood Flow &
Metabolism. Eds. F. Gotoh, H. Nagai and Y. Tazaki. Hidejima Seihanjo, Japan, 1979, pp. 330-331.

94. Winn, H. R., J.E. Welsh, C. Bryner, R. Rubio & R. M. Berne. Brain adenosine production during the initial 60 seconds of bicucculline seizures in rats. Proceedings of the IXth International
Symposium of Cenrebral Blood Flow & Metabolism. Eds. F. Gotoh, H. Nagai and Y. Tazaki.
Hiedejima Seihanjo, Japan, 1979, 00. 536-537.
95. Winn, H. R., R. Rubio, and R. M. Berne. Brain Adenosine Production in the Rat during 70 seconds of Ischemia. Circulation Research, 45: 486-492, 1979.
96. Belardinelli, L., S.M. Vogel, N. Sperelakis, R. Rubio and R. M. Berne. Restoration of Slow
Responses in Hypoxic Heart Muscle by Alkaline pH. J. Mol. Cell. Cardiol. 11: 877-892, 1979.
97. Miller, W. L., L. Belardinelli, A. Bacchus, D. H. Foley, R. Rubio and R. M. Berne. Canine
Myocardial adenosine and lactate production, oxygen consumption and coronary blood flow during stellate ganglia stimulation. Circ.Res. 45: 708-718, 1979.
98. Thompson, C.l., R. Rubio and R.M. Berne. Changes in adenosine and glycogen phosphorylase activity during the cardiac cycle. Am. J. Physiol. 238: H389-H398,1980.
99. Tominaga, S., R.R. Curnish, L. Belardinelli, R. Rubio and R.M. Berne. Adenosine release during early and sustained exercise of canine skeletal muscle. Am. J. Physiol. 238: H156-H163,
1980.
100. Winn, H.R., T.S. Park, R.R. Curnish, R. Rubio and R.M. Berne. Incorporation of adenosine and its metabolites in brain nucleotides. Am. J. Physiol 239: H212-H219, 1980.
101. Rubio, R. and R.M. Berne. Localization of nucleoside phosphorylases selective for purine and pyrimidine compounds in heart, kidney and liver. Am. J. Physiol. 239: H721-H730, 1980.
102. Winn, H.R., J.E. Welsh, R. Rubio, and R.M. Berne. Changes in brain adenosine during bicuculline-induced seizues in rats. Circ. Res. 47: 568-577, 1980.
103. Winn, H.R., J.E. Welsh, R. Rubio, and R.M. Berne. Brain adenosine production in rat during sustained alteration in systemic blood pressure. Am. J. Physiol. 239: H636-H641, 1980.
104. Belardinelli, L., F.L. Belloni, R. Rubio and R.M. Berne. Atrioventricular conduction disturbances during hypoxia: possible role of adenosine in rabbit and guinea pit heart. Circ. Res.
47: 684-691,1980.
105. Rubio R., R.M. Berne, P. Silva and D. Erlij. Effects of stimulating secretion on purine derivatives in the rectal gland and blood of the dogfish. Bulletin MDIBL 20:143-145, 1980.
106. Erlij, D., P. Silva, R. Rubio. Effects of adenosine analogues on secretion by the isolated rectal- gland of the dogfish, Squalus Acanthias. Bulletin MDIBL 20:145-146, 1980.
107. Erlij, D., R. Rubio, and P. Silva. Isotonic solutions stimulate secretion by the "in situ" rectal gland of the dogfish. Bulletin MDIBL 20:141-143, 1980.
108. Kuehl, K.K., S.P. Bruttig, D.V. Singer, R. Rubio and R.M. Berne. Growth of aortic vascular smooth muscle cells in lowered oxygen tension. Cell Tissue Res. 216: 591-602, 1981.
109. Berne, R.M., H.R. Winn, and R. Rubio. Metabolic regulation of cerebral blood ftow. In:
Vasodilation, edited by P.M. Vanhoutte and 1. Leusen. Raven Press, New York, 1981, pp . 231 -
241.
110. Winn, H.R., R. Rubio, and R.M. Berne. Brain adenosine concentration during hypoxia in rats.
Am. J. Physiol. 241: H235-H242, 1981.
111. Winn, H.R., R. Rubio, and R.M. Berne. The role of adenosine in the regulation of cerebral blood flow. J. Cerebral Blood Flow Metab. 1: 239-244, 1981.
112. Fenton, R.A., R. Rubio, and R.M. Berne. Adenosine and the acid-base state of vascular smooth muscle. J. Appl. Physiol. 51: 179-184, 1981.
113. Berne, R.M., H.R. Winn, and R. Rubio. The local regulation of cerebral blood flow. Prog.
Cardiovasc. Diseases 24: 243-260, 1981.
114. Berne, R.M. F.L. Belloni, and R. Rubio. Adenosine associated with dispersed hepatocytes.
Basic Res. Cardiol. 76: 377-379, 1981.
115. Berne, R.M., H.R. Winn, T.S. Park, J.E. Welsh, R.R. Curnish, and R. Rubio. Effect of adenosine and an adenosine analogues on canine cerebral blood flow. IN: Cerebral
Microcirculation and Metabolism. Eds. J. Cervos Navarro and E. Fritschka, Raven Press, New
York, 1981, pp. 227-232.
116. Winn, H.R., J.E. Welsh, R.M. Berne and R. Rubio. The effects of hypoxia and hypotension on adenosine production during bicuculline-induced seizures in brain. In: Cerebral Micro-circulation and Metabolism eds. J. Cervos-Navarro and E. Fritschka, Raven Press, New York, 1981, pp. 249-
254.
117. Berne, R.M. and R. Rubio. The regulation of coronary blood flow. In Coronary Artery Disease.
Eds. William Santamore and Alfred Bove. Urban and Schwarzenberg, Baltimore, 1982, pp. 39-59.

118. Berne, R.M., I. Thompson, L. Miller, D.H. Foley, P. Watkinson, and R. Rubio. The effect of increases in cardiac oxygen need on adenosine formation and coronary blood flow in the steady state and during the cardiac cycle. In: Microcirculation of the Heart. Ed.s. H. Tillmanns, W. Kubler, and H. Zebe, Springer-Verlag, 1982, pp. 39-48.
119. Fenton, R.A., S.P. Bruttig, R. Rubio and R.M. Berne. Effect of adenosine on calcium uptake by Intact and cultured vascular smooth muscle. Am. J. Physiol. 242: H797-H304, 1982.
120. Winn, H.R., R. Rubio, and R.M. Berne. Changes in brain adenosine during short lasting (30 sec) hypoxia. J. Cerebral Blood Flow Metab. 1: S399-S400, 1981.
121. Winn, H.R., R.-Rubio, R.R. Curnish, and R.M. Berne. Changes in regional cerebral blood flow
(rCBF) caused by increases in CSF concentrations of adenosine and 2-chloroadenosine (CHL-
AD0). J. Cerebral Blood Flow Metab. 1: S401-S402, 1981.
122. Berne, R.M., H.R. Winn, and R. Rubio. The effect of inadequate oxygen supply to the brain on cerebral adenosine levels. IN: Cerebral Hypoxia in the Pathogenesis of Mioraine. Eds. F.C. Pose and W.K. Amery, Pitman Press, England, 1982, pp 82-91.
123. Bacchus, A.N., S.W. Ely, R.M. Knabb, R. Rubio and R.M. Berne. Adenosine and coronary blood flow in conscious dogs during normal physiological stimuli. Am. J. Physiol. 243: H628-H633,
1982.
124. Berne, R.M., L. Belardinelli, H.R. Winn, and R. Rubio. The adenosine concept in ischemia of heart and brain. IN: Protection of Tissue Against Hypoxia. Eds. A. Wauquier et al., Janssen
Research Foundation Symposium. Elsevier Biomedical Press, Amsterdam, 1983. pp 105-114.
125. Knabb, M.T., R. Rubio, and R.M. Berne. Potentiation of slow action potentials with theophylline or "micro" adenosine deaminase. Am. J. Physiol. 244: H454-H457, 1983.
126. Berne, R.M., H.R. Winn, R.M. Knabb, S.W. Ely, and R. Rubio. Blood flow regulation by adenosine in heart, brain and skeletal muscle. IN: Regulatory Function of Adenosine. Eds. R.M.
Berne, T.W. Rall and R. Rubio, Martinus Nijhoff, Publishers, 1983, pp. 293-317.
127. Rubio, R., M.T. Knabb, T. Tsukada and R.M. Berne. Mechanisms of action of adenosine on vascular smooth muscle and cardiac cells. IN: Regulatory Function of Adenosine. Eds. R.M. Berne.
Rall T. and R. Rubio. Martinus Nijhoff Publishers, 1983, pp 319-332.
128. Knabb, R.M., S.W. Ely, A.N. Bacchus, R. Rubio, and R.M. Berne. Consistent parallel relationships among myocardial oxygen consumption, coronary blood flow, and pericardlal infusate adenosine concentration with various interventions and §-blockade in the dog. Circ. Res. 53: 33-
41, 1983.
129. Ely, S.W., R. Knabb, A.N. Bacchus, R. Rubio, and R.M. Berne. Measurements of coronary plasma and pericardial infusate adenosine concentrations during exercise in conscious dog:
Relationship to myocardial oxygen consumption and coronary blood flow . J. Molec. Cell Cardiol.
15: 673-683, 1983.
130. Berne,R.M., R.M. Knabb, Ely,S.W., and R.Rubio. Adenosine in the local regulation of blood flow. A brief overview. Fed. Proc. 42:3136-3142, 1983.
131. Berne, R.M., S.W. Ely, R.M. Knabb, A. BAcchus, and R. Rubio. Local regulation of coronary flow in normoxia. Proceedings of Ca++ blockers, Adenosine and Neurohumors: Advances in
Coronary Vascular and Cardiac Control. Rutgers University, eds,G.F. Merrill, H.R. Weiss &A.
Scriabine, Urban & Schwarzenberg, Baltimore.1983. ppl77-188.
132. Winn. R.H., Rubio, R., R.M. Berne.Metabolic fate of adenosine and its metabolites in cerebral spinal fluid. Neurobiology of cereral spinal fluid ll. ed..J.H. Wood, Plenun Press, New York. 1983. pp591-601.
133. Ely, S.W., R.M. Knabb,R. Rubio, and R.M. Berne. Comments on pericardial superfusion to measure cardiac interstitial adenosine concentration. Amer. J. Physiol. 245: H1085-H1088, 1983.
134. Ely, S.W., Sun,Chi-Wen, Knabb, R.M., J.M. Gidday, R. Rubio, R.M. Berne.Adenosine and metabolic regulationof coronary blood flow in the dog with renal hypertension. Hypertension 5:
943-950,1983.
135. Belloni, F.L., R. Rubio, and R.M. Berne.lntracellular adenosine in isolated rat liver cells.
Pflugers Arch.400:106-108, 1984.
136. Tsukada, T. Rubio, R., and Berne, R.M. Pharmacological differentiation between large and small coronary vessels. Arch. Internat. Pharmacol. Therap. 270: 255-267, 1984.
137. Rubio, R. Significado funcional de la liberation de adenosina por las cellulas cardiacas. In
"FISIOLOGIA Y PATOLOGIA DE LOS MUSCULOS CARDIACO Y ESQUELETICON. eds, G.,
Pastelin, y J. Mu–os. Mex. Fisiol. Soc. 1984
138. Knabb,M.T., R. Rubio,and R.M. Berne. Calcium dependent atrial action potentials generated

with phophatidic acid or phospholipase D. Pflugers Arch. 401: 435-437, 1984.
139. Knabb, R.M., Gidday, J.M., Ely, S.W., Rubio, R., Berne,R.M. Effects of dipyridamole on myocardial adenosine and active hyperemia. Amer. J. Physiol.247: H804-H810, 1984.
140. Tsukada, T., Rubio, R., Berne, R.M. Effects of chronic denervation on pharmacological responsiveness of coronary vessels. J. Autonom. Nerv. Svst. 13: 49-64, 1985.
141. Erlij, D. and Rubio, R. Control of secretion by the rectal gland of the dogfish (Squalus
Acanthias): Steps in the sequence of activation. J. Exp. Biol. 122: 99-112, 1986.
142. Berne, R.M., Curnish, R.R., Gidday, J.M., and Rubio, R. Measurement of femtomolar concentrations. of adenosine. J. Lig. Chromat. 9:113-119, 1986.
143. Bencherif, M., Berne, R.M., and Rubio, R. The release of purines by the frog sympathetic ganglion is the result of activation of postsynaptic elements. J. Physiol. 371: 274P, 1986.
144. Berridge, M. and Rubio, R. Inhibition by extracellular K+ (K+o) on the rate of Pl turnover in the
5-HT stimulated blow-fly salivary gland. J. Physiol. 371: 225P, 1986.
145. Van Wylen, D.G.L., Park, T.S., Rubio, R. and Berne, R.M. Increases in cerebral interstitial fluid adenosine concentration during hypoxia, local potassium infusion, and ischemia. J. Cerebral
Blood Flow and Metabolism. 6: 522-528, 1986.
146. Shryock, J.C., R. Rubio and R.M. Berne. Extraction of adenosine nucleotides from c ultured endothelial cells. Analytical Biochem. 159:73-81, 1986.
147. Belloni, F., S.P. Bruttig, R. Rubio and R.M. Berne. Release of adenosine by cultured vascular smooth muscle cells. J. Microvasc. Res. 32:200-210,1986.
148. Rubio, R. and Bencherif, M. Adenosine sites of release in the nervous system and control of phosphatidylinositol turnover. In "Cardiac Electrophysiology and Pharmacology of Adenosine and
ATP. Basic clinic aspects. Ed. E. Pelleg. Alan R. Liss Publishers, 1987.
149. Berne, R.M., Gidday, J.M., Hill, E.H., Curnish, R.R., and Rubio, R. The interstitial fluid adenosine concentration during altered cardiac metabolism in the dog. In Cardiac electrophysiology of adenosine and ATP: Basic and Clinical Aspects. Ed. E. Pelleg. Alan R. Liss
Publishers, 1987.
150. Rubio, R., Knabb, R.M., Ely, S.W., and Berne, R.M. A critique on the use of adenosine deaminase to test the adenosine hypothesis: Disregarded implicit assumptions. In "Regulatory
Function of Adenosine." Eds. E. Gerlach, G.W. Kreutzberg, and J. Schrader. Springer-Verlag
Publish. 1987.
151. Park, T.S., Van Wylen, D.G.L., Rubio, R., and Berne, R.M. Increased brain interstitial fluid adenosine concentration during hypoxia in newborn piglet. J. Cerebral Blood Flow and
Metabolism, 7:178-183, 1987.
152. Park, T.S., Van Wylen, D.G.L., Rubio, R., and Berne, R.M. Interstitial fluid adenosine and sagttal sinus blood flow during bicuculline siezures in newborn piglets. J. Cer. Blood Flow and
Metabolism. 7:633-639, 1987.
153. Park, T.S., Van Wylen, D.G.L., Rubio, R., and Berne, R.M. Effect of hypoxia on interstitial adenosine concentrations in the newborn piglet. Concepts in Pediatric Neurosurgery. 8:111-119,
1988.
154. Mainwaring, R., B.S. Lasley, R. Rubio, D.A. Wyatt and R.M. Mentzer. Adenosine stimulates glucose uptake in the rat heart. Surgery 10:445-449,1988.
155. Gidday, J.M., H.E. Hill, R. Rubio and R.M. Berne. Estimates of left ventricular interstitial fluid adenosine during catecholamine stimulation. Amer. J. Physiol. 254:H207-216,1988.
156. Shryock, J.C., R. Rubio and R.M. Berne. Release of adenosine from pig aortic endothelial cells during hypoxia and metabolic inhibition. Amer. J. Physiol. 254:H223-H229,1988.
157. Van Wylen, D.G.L., T.S. Park, R. Rubio and R.M. Berne. Cerebral Blood flow and interstitial fluid adenosien during hemorrhagic shock. Amer J. Physiol. 255:H1211-H1218. 1988.
158. Meghji, P., R. Rubio and R.M. Berne. Intracellular adenosine formation and its carriermediated release in cultured embryonic heart cells. Life Sciences. 43:1851-1859, 1989.
159. Rubio, R., M. Bencherif and R.M. Berne. Release of purines from postsynaptic structures of amphibian ganglia. J. Neurochem. 51:1717-1723,1988.
160. Rubio, R., M. Bencherif and R.M. Berne. Metabolism of phosphoinositides during synaptic activation: Identification of stimulating and inhibiting agonist. J. Neurochem. 52:797-806,1989.
161. Meghji, P. R. Rubio and J Tuttle. Adenosine formation and release by embryonic chick neurons and glia in cell culture. J. Neurochem. 53:1852-1860,1989.
162. Wyatt, A.D., M.C. Edmunds, R. Rubio, R.M. Berne, R.D. Lasley and R.M. Berne. Adenosine stimulates glycolitic flux in isolated perfused rat heart by A1-adenosine receptors. Amer. J.

Physiol. 257:1952-1957,1989.
163. Gidday JM., Rubio, R., Berne RM. Effects of Theophylline on myocardial reactive hyperaemias in the anaesthetized dog. Brit. J Pharmacology 100: 95-101, 1990.
164. Tietjan, C.A., C.G. Tribble, J.M. Gidday, C.L. Phillips, L. Belardinelli, R. Rubio and R. M.
Berne. Interstitial fluid adenosine concentration estimates in isolated guinea pig hearts using epicardial porous discs. Amer. J. Physiol. 259: H1471-H1479,1990
165. Gidday, J.M., Kaiser, D.M., Rubio, R. and Berne, R.M. Heterogeneity of steady-state epicardial adenosine concentration and its dependence on the volume utilized for sampling. J. Mol.
Cell Cardiol. 24: 351-364, 1992.
166. Gidday, J, M., Kaiser, D.M., Rubio, R. and Berne, R.M. Interstitial adenosine: The measurement, The interpretation. J. Mol. Cell. Cardiol. 24: 347-350, 1992.
167. Balcells, E., Suarez, J. and Rubio, R. Functional role of intravascular coronary endothelial adenosine receptors. Europ. J. Pharmacol. 210:1-9,1992
168. Suarez, M.J. and Rubio, R. Regulation of glycolytic flux by coronary flow in guinea pig heart.
Role of vascular endothelial shear stress. Amer. J. Physiol. 261: Hl994-H2000,1991.
169. Balcells, E., Suarez, J. and R. Rubio. Implications of the coronary endothelium as mediator of the vasodilatory and dromotropic actions of adenosine. J. Mol. Cell Cardiol. 25: 693- 706, 1993.
170. Ceballos G., J. Tuttle and R. Rubio. Differential distribution of purine degrative pathways between glial and neuronal cells in culture. J. Neurochem. 62:1144-1153 (1994).
171. Torres, M., G. Ceballos and R. Rubio. Cathecholamine secretion by the chromaffin cells is controlled by cultured endothelial cells. J. Neurochem. 63:988-996,1994
172. Rubio, R., E. Balcells, G. Ceballos, J. Suarez and M. Torres. Endothelial-mediated regulation of cardiac and chromaffin cell functions. In ÒFunctionality of the endothelium in health and diseased statesÓ. Ed. R. Rubio, G. Pastelin, G. Ceballos and J. Suarez. Soc. Mex. Cardiol.
Veracruz Univ. Press. 1994
173. Ceballos, G. and R. Rubio. Endothelial-glial complexing in culture: A specialized system for transport of purines. In “Functionality of the endothelium in health and diseased states”. Ed. R.
Rubio, G. Pastelin, G. Ceballos and J. Suarez. Soc. Mex. Cardiol. Veracruz Univ. Press. 1994
174. Ceballos G. and R. Rubio. Co-culture of astroglial and vascular endothelial cells as apposing layers enhances the transcellular transport of hypoxanthine. J. Neurochem. 64:991-999,1995
175. Rubio, R., G. Ceballos and J. Suarez. Coronary flow regulates Auricular Ventricular delay in the isolated perfused guinea pig heart. Amer. J. Physiol. 269:H1177-H1185,1995.
176. G. Ceballos, R. Rubio, R. Yánez, G. Chamorro : ESTUDIO DEL TRANSPORTE Y METABOLISMO
DE HIPOXANTINA EN UN SISTEMA DE COCULTIVOS DE ENDOTELIO VASCULAR Y ASTROCITOS EN
APOSICION CERCANA . ACTA MEDICA, VOL. XXXI. NUM. 122- PP. 29-45, 1996.
177. Ceballos, G. and R. Rubio. Endothelium-mediated negative dromotropic effects of intravascular acetylcholine. Europ. J. Pharmacol. 362:157-166, 1998.
178. Rubio, R., G. Ceballos, and E. Balcells. Intravascular adenosine: The endothelial mediators of its negative dromotropic effects. Europ. J. Pharmacol. 370:27-37, 1999.
179. Rubio, R.., and G. Ceballos. Role of endothelial glycocalyx on the dromotropic, inotropic and arrithmogenic effects of coronary flow. Amer. J. Physiol. 278:106-H116, 2000.
180. Zenteno S. T., Ceballos G., and R. Rubio. Effects of arginine vasopressin in the heart are mediated by specific intravascular endothelial receptors. Europ. J. Pharmacol. 410:15-23, 2000.
181. Rubio, R., and G. Ceballos. Functional implications of sole and selective activation of intravascular coronary endothelial hormonal receptors. Acta Pharmacologica Sinica. 21:577-586,
2000.
182. Rubio, R., and G. Ceballos. Sole activation of three luminal adenosine receptors subtypes in different parts of the coronary vasculature. Amer J. Physiol. 284:H204-H214, 2003
183. Gonzalez-Castillo, C., R. Rubio and Zenteno-Savin, T.
Coronary flow-induced inotropism is modulated by binding of dextrans to the endothelial luminal surface. Amer. J. Physiol. 284:H1348-
H1357, 2003
184. Castillo-Hernandez, J., Rubio-Gayosso, I., Sada-Ovalle, I., Garcia-Vasquez, A., Ceballos G., and R. Rubio. Intracoronary Angiotensin II causes inotropic and vascular effects via different paracrine mechanisms. Vascular Pharmacology. 41/4-5, 147-158, 2004
185. Gonzalez C., Cobacho AM., Eiserich J., Barrera-Lopez F., Morales-Tlalpan V., Barajas-
Espinosa A., Diaz-Munoz M., Rubio R., Lin S-H., Martinez de la Escalera G. and Clapp C.16Kprolactin inhibits activation of endothelial nitric oxide synthase, calcium mobilization and endothelium-dependent vasorelaxation. Endocrinology. 145:5713-5722, 2004

186. Rubio-Gayosso I., Barajas-Espinosa A., Castillo-Hernandez J., Ramiro-Diaz J., G. Ceballos and R. Rubio. Enzymatic hydrolisis of luminal coronary glycosidic structures uncovers their role in sensing coronary flow. Frontiers in Bioscience 10:1050-1059, 2005
187. Juan P. Reyes, R. Espinosa-Tanguma, M. A. Basurto, U. Meza, P. Perez-Cornejo, J. Arreola,
R. Rubio. Role of endotelial integrins in flow transduction; A review ans a novel experimental approach. Episteme 8-9; Oct. 2006. http://www . Uvmnet.edu/investigación/episteme
188. Guevara, G., Rubio-Gayosso, I., Miliar-Garcia, A., Castillo, C., Ramirez-Sanchez, I., Meaney,
E., Meaney, A., Rubio, R., Ceballos, G. Mannose polymer induces vasodilation through a luminal mannose receptor in mesenterio arteries. Frontiers in Bioscience. 5294-5303, May 1, 2008.
189. Barajas-Espinosa Alma, Juan Ramiro-Diaz, Erika Briones-Cerecero, Erika Chi-Ahumada, Ana
Barba de la Rosa, Blanca Arroyo-Flores, Rafael Rubio. Involment of endotelial Man- and Galbinding lectins in sensing the flow in coronary arteries. Frontiers in Bioscience. 5421-5431, May 1,
2008.
190. Jesús Castillo-Hernández, David Torres-Tirado, Erika Chi-Ahumada, Alma Barajas-Espinosa,
Juan Ramiro-Díaz, Guillermo Ceballos, Rafael Rubio. Two dissimilar AT
1
agonists distinctively activate AT1 receptors located on the luminal membrane of coronary endothelium. Vascular
Pharmacology 51:314-322, 2009.
191. Juan Ramiro-Diaz
1
, Alma Barajas-Espinosa
1
, Erika Chi-Ahumada
1
, David Torres-Tirado
1
,
Jesus Castillo-Gonzalez
1
, Ana Barba de la Rosa
2
, Rafael Rubio. Luminal endothelial lectins with affinity for N-Acetylglucosamine determine flow-induced cardiac and vascular paracrine-dependent responses. Amer. J. Physiol. Submitted.
MANUSCRIPTS READY FOR SUBMISSION AND IN PREPARATION:
192. Blanca Arroyo-Flores, Erika Briones-Cerecero, Erika Chi-Ahumada, Ana Barba de la Rosa, Maureen
Knabb, Rafael Rubio.
Changes in protein profile composition of coronary luminal membrane induced by ischemia and ischemia/reperfusion
193.
Maureen Knabb, Sandra Pérez-Aguilar, David Torres-Tirado, Juan Ramiro-Díaz, Rafael
Rubio. Biochemical alterations in coronary endothelial luminal membranes during ischemia and reperfusion in isolated perfused guinea pig hearts
194. David Torres-Tirado, Jesús Castillo-Hernández, Erika Chi-Ahumada, Ramiro-Diaz Juan,
Rafael Rubio. The coronary endothelium as a functional and physical barrier for endocrine
Angiotensin II.
195. González C
1*
, Martínez-Cuevas P
1
, Velarde-Salcedo J
1
, Rosas-Hernández H
1
, Fudizawa R
1
,
Castillo-Hernández JR
2
, Ramiro-Diaz JM
2
, and Rubio R. Vasoinhibins block Endothelial Nitric
Oxide-dependent vasodilatation induced by Growth hormone and Placental Lactogen: A Potential
Feedback Mechanism
196. Sandra Perez-Aguilar, David Torres-Tirado, Juan Ramiro-Diaz, Maureen Knabb, Rafael Rubio
.
Isolation and characterization of lectins from the coronary luminal membrane
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