OPERATOR’S MANUAL
BIOPROtect III / -LE
Vent to Room / Vent to C.E.C /
Vent to H.E.C
Bio-Containment Enclosure
This manual includes information for installation, operation, maintenance and spare parts.
We recommend that it be kept near the cabinet for ready reference.
March, 2009
Rev B
THE BAKER COMPANY
161 Gatehouse Rd., Sanford, Maine 04073 USA ⋅ (207) 324-8773 ⋅ 1-800-992-2537 ⋅ FAX (207) 324-2632
Internet address: http// www.bakerco.com
“Creating Immaculate Atmospheres”
The Baker Company
INTRODUCTION AND WELCOME
It is a pleasure to welcome you to the growing number of customers who own and operate Baker
biological safety cabinets. As the inventors of the laminar flow biological safety cabinet and the leaders in
the field, Baker people take special pride in providing a cabinet that is designed for maximum performance.
Your new BIOPROtect III cabinet includes many unique features which are included to give you superior
performance, simpler maintenance and lower life cycle cost. Your BIOPROtect III cabinet is designed for
both safety and value.
In addition to the high quality you expect from all Baker equipment, this model has been ergonomically
designed to provide the lab user with many exciting design features. The revolutionary ergonomic design
will help prevent repetitive motion injury, reduce fatigue and lab accidents and enhance productivity.
You will find your BIOPROtect III cabinet suitable for use not only for research and clinical diagnostic
work involving tissue culturing of possibly infectious samples, but also for I.V. drug preparations and other
pharmaceuticals that could have adverse health effects on operators and other techniques requiring a
contamination-free atmosphere.
Please note that all open-front containment cabinets, including this one, are for use with low to moderate
risk agents only. Open-front cabinets do not provide absolute protection for the user. The adequacy of a
cabinet for user safety should be determined on-site by an industrial hygienist, safety officer or other
qualified person. Remember that you, the owner and user, are ultimately responsible and that you use your
cabinet at your own risk.
We recommend that this manual, along with factory test report, be kept near the cabinet for convenient
reference by operators and qualified maintenance personnel. If you have any questions about the use or
care of your new BIOPROtect III cabinet, please do not hesitate to contact our Customer Service
Department at 800-992-2537 for assistance or e-mail us at bakerco@bakerco.com.
Sincerely,
Dennis Eagleson
President, CEO
The Baker Company, Inc.
P.O. Drawer E, Sanford, Maine 04073 (207) 324-8773 1-800-992-2537 FAX (207) 324-3869
Visit our website at www.bakerco.com
Table of Contents
INTRODUCTION AND WELCOME ................................................................................................ i
Table of Contents ............................................................................................................................... ii
I - FUNCTION AND DESCRIPTION OF THE BIOPROtect III/-LE CABINETS ......................... 1
Airflow Inside the BIOPROtect III/-LE Cabinets .......................................................................... 1
Access to the Work Area................................................................................................................ 2
Design Details ................................................................................................................................ 3
Performance assurance.............................................................................................................. 3
All-metal plenums..................................................................................................................... 3
Tested HEPA filters .................................................................................................................. 3
Work area lighting .................................................................................................................... 3
Cable Port.................................................................................................................................. 3
Optional Height Adjustable Cart .................................................................................................... 3
Specifications ................................................................................................................................. 4
Dimensions: .............................................................................................................................. 4
Weights ..................................................................................................................................... 4
Utilities: .......................................................................................................................................... 4
Mechanical (Optional) .............................................................................................................. 4
Electrical ................................................................................................................................... 4
Environmental Conditions.............................................................................................................. 5
Symbols and Terminology ............................................................................................................. 5
II - PREPARING THE BIOPROtect III/-LE CABINET FOR USE.................................................. 6
The Uses of the BIOPROtect III/-LE. ............................................................................................ 6
Location Within the Laboratory ..................................................................................................... 6
III - PROPER USE OF CABINETS...................................................................................................7
Operator Controls: .......................................................................................................................... 7
Blower On / Off Switch – ........................................................................................................ 7
Light On / Off Switch – ........................................................................................................... 7
Mute switch - ............................................................................................................................ 7
Left Side & Right Side Outlet On / Off – ................................................................................. 8
Ground Fault Circuit Interrupter .................................................................................................... 8
Air Pressure Alarm ......................................................................................................................... 8
Door Operations ............................................................................................................................. 9
To open the viewscreen: ........................................................................................................... 9
To open the doors (the right side door must be opened first): .................................................. 9
Removing the cart .......................................................................................................................... 9
Set-up Procedure .......................................................................................................................... 10
Start-up procedure ........................................................................................................................ 11
Reacting to Spills.......................................................................................................................... 12
Decontamination .......................................................................................................................... 13
About the HEPA Filters ............................................................................................................... 14
IV- ON-SITE CHECKS AND MAINTENANCE PROCEDURES ................................................ 15
Filter Media and Seal Leak Tests ................................................................................................. 15
Procedure for filter leak tests........................................................................................................ 15
The Airflow Balance .................................................................................................................... 16
Airflow Smoke Pattern Test ......................................................................................................... 17
Grounding Continuity Test........................................................................................................... 17
Calibration procedure for Modus Airflow alarm(Digital Differential Pressure Monitor). .......... 18
Initial Set Point Settings.......................................................................................................... 18
Low Flow Alarm Setting ........................................................................................................ 18
Check the Calibration ............................................................................................................. 18
Modus Programming Instructions ................................................................................................ 19
1.) Programming the Alarm Set points ................................................................................... 19
2.) Programming the Relay Dead band................................................................................... 19
3.) Returning to Normal Operation......................................................................................... 19
Maintenance Notes ....................................................................................................................... 20
Replacing the HEPA Filter........................................................................................................... 20
Changing the Exhaust Filter.................................................................................................... 20
Changing the Supply Filter ..................................................................................................... 21
V.- TROUBLE SHOOTING ........................................................................................................... 22
REPLACEMENT PARTS LIST .................................................................................................. 24
VI.- APPENDIX............................................................................................................................... 25
Modus Installation and Operation Handbook............................................................................... 26
BGA-BIOPROtect III Vent to Room Page 1 ............................................................................... 40
BGA-BIOPROtect III Vent to Room Page 2 ............................................................................... 41
BGA-BIOPROtect III Vent to Room Page 3 .............................................................................. 42
BGA-BIOPROtect III-19 Hard Exhaust Connection(HEC) Page 1............................................. 43
BGA-BIOPROtect III-19 Hard Exhaust Connection(HEC) Page 2............................................. 44
BGA-BIOPROtect III-19 Hard Exhaust Connection(HEC) Page 3............................................. 45
BGA-BIOPROtect III-20 Canopy Exhaust Connection (CEC) Page 1 ....................................... 46
BGA-BIOPROtect III-20 Canopy Exhaust Connection (CEC) Page 2 ....................................... 47
BGA-BIOPROtect III-20 Canopy Exhaust Connection (CEC) Page 3 ....................................... 48
BGA-BIOPROtect III-LE Vent to Room Page 1..........................................................................49
BGA-BIOPROtect III-LE Vent to Room Page 2..........................................................................50
BGA-BIOPROtect III-LE Vent to Room Page 3..........................................................................51
BGA-BIOPROtect III-LE Hard Exhaust Connection (HEC) Page 1............................................52
BGA-BIOPROtect III-LE Hard Exhaust Connection (HEC) Page 2............................................53
BGA-BIOPROtect III-LE Hard Exhaust Connection (HEC) Page 3............................................54
BGA-BIOPROtect III-LE Canopy Exhaust Connection (CEC) Page 1........................................55
BGA-BIOPROtect III-LE Canopy Exhaust Connection (CEC) Page 2........................................56
BGA-BIOPROtect III-LE Canopy Exhaust Connection (CEC) Page 3........................................57
BGA-BIOPROtect III Optional Cart ............................................................................................ 58
Wiring Diagram-Standard BIOPROtect III.................................................................................. 59
WARRANTY ...............................................................................................................................60
Test Report…....………………….……………………………………………………….Attached
I - FUNCTION AND DESCRIPTION OF THE BIOPROtect III/-LE CABINET
The BIOPROtect III and BIOPROtect III-LE is a Class II Bio-Containment Enclosure designed to house
laboratory robotics or large apparatus that would not ordinarily fit in a standard Class II Biological Safety
Cabinet (BSC). Some important features of the BIOPROtect III/-LE include, vertical laminar airflow, a front
access opening, as well as supply & exhaust HEPA filters. The unit is designed to protect not only the
environment and the people using the cabinet, but also the product within from airborne contaminants. The
BIOPROtect III/-LE can be configured to vent three different ways. Vent to the room, Vent to a Canopy
Exhaust Connection (C.E.C.) via a house exhaust system or, Vent to a Hard Exhaust Connection (H.E.C.) via
a house exhaust system. Although your unit has passed a microbiological test, a modification of NSF/ANSI
standard-49-2002e, the BIOPROtect III/-LE is not currently listed with the NSF.
Airflow Inside the BIOPROtect III/-LE Cabinet
Figure 1 shows the name and location of component parts and the typical airflow pattern of the
BIOPROtect III/-LE.
The stainless steel metal diffuser (1) just below the supply HEPA (High Efficiency Particulate Air) filter
assures that the work area is bathed in HEPA filtered air. The supply air stream splits so that some is
directed into the front grille (2) and the balance is pulled into the perforated grilles on the rear wall (3). On a
flat table top, in the absence of any obstacles, the air should split roughly in the center of the table.
Because the cabinet must take in air to replace the air which is expelled through the exhaust filter, the
same quantity of room air enters the cabinet through the eight inch front access opening. The flow of the
intake air provides the personnel protection (containment) aspect of the cabinet.
The front grille of the cabinet is integral to the large hinged doors. These doors, as well as the side and
rear walls, are maintained under a negative pressure. Air is drawn from the front intake grilles, into the
hollow doors, and into the side walls. In this manner the outside air that is drawn into the cabinet as intake is
not allowed to penetrate the table top area of the cabinet.
Any and all contaminated air that is captured in the cabinet’s negative pressure plenums (front doors,side
walls, rear walls or upper plenum) is pulled into the cabinet’s blower (4). The blower discharges into a steel
box that is a common plenum for the supply and exhaust HEPA filters (5). In this manner, all the air that is
handled by the cabinet is HEPA filtered. The BIOPROtect III/-LE will exhaust a quantity of air (6) equal to
that received at the front intake grilles (2). If a leak should occur in a contaminated pressure plenum the
negative pressure will create suction and pull air in, not allowing it to escape into the laboratory. If there is a
leak from the positive pressure plenum, the surrounding negative pressure area will recapture the
contaminated air and recirculate it through a HEPA filter.
Page 1 of 60
Figure 1 Typical section with airflow visualization arrows
Access to the Work Area
The BIOPROtect III/-LE is provided with three modes of access to the work area; via the large hinged
doors that can be opened to access the entire work area, the hinged viewscreens (providing access to the
work surface) or from the 8” x 42” access (sash) openings between the bottom of the closed viewscreens and
the air intake grates. The primary means of access must be through the 8” sash openings. This is the only
means of access that will ensure the user protection from contaminants within the enclosure.
The operator is only protected from airborne particulate when the large front doors and the hinged viewscreens
are closed and secured to provide the 8”x 42” access (sash) openings
For easy set-up of equipment in the work area, the BIOPROtect III/-LE has two large doors that exposes
the entire work area from floor to ceiling to an area of 102” wide x 75” tall (in the BIOPROtect III) and
102” wide x 65” tall (in the BIOPROtect III-LE).
Page 2 of 60
Design Details
Performance assurance
Meticulous care in manufacturing is followed by more than 14 separate performance tests prior to
shipment of your cabinet. In addition, a complete factory test report on the operation of your unit is
included at the rear of this manual.
All-metal plenums
The work area surfaces and plenums of your BIOPROtect III have been constructed entirely of metal
in order to provide strength, durability, air-tightness and resistance to deterioration. External
construction is of 16-gauge cold-rolled steel, protected by a smooth white baked enamel finish.
Tested HEPA filters
Supply and exhaust HEPA filters in the BIOPROtect III unit have been found to be 99.99 percent
effective on particles of 0.3 micron size. Each filter is scan-tested to assure leak-free installation.
Work area lighting
The fluorescent lights are externally mounted within the light canopy to minimize heat buildup in the
work area. The unit uses four common F54T5 fluorescent lamps powered by an efficient and reliable
electronic ballast.
Cable Port
A 2.75” I.D. cable port is provided in each side wall to accommodate communications ports from
process equipment without compromising performance.
Optional Height Adjustable Cart
The all stainless steel cart, which is optional on the BIOPROtect III/-LE was designed to accommodate
process equipment weighing no more than 500 Lbs. It’s work surface dimensions are 90” Wide x 34” Deep.
The height of the worksurface can be adjusted by the operator from 27” to 39” by using the manual crank
provided at the front of the cart. For ideal ergonomic performance the cart should be adjusted so that the
work table of the equipment is at the same level as the front intake grille of door on the front of the cabinet.
The cart is provided with swivel casters, all of which are locking. To retract the cart from the work area,
tip the foot operated locking devices at each front caster to a horizontal position then pull the cart out of the
work area. To return the cart to the cabinet push the cart back into the work area and lock the front casters
by pushing down on the foot operated locking devices. Make certain that the crank handle for the lift system
is facing the front of the cabinet and is retracted under the work table before closing the door.
Page 3 of 60
Specifications
Dimensions: BioProtect III
Outside:
116.88”W x 50.00”D (FOOT PRINT) x 102.50”H (over junction boxes)
Usable work area: 102”W x 36.5”D x 75”H (under diffuser)
Cart worksurface: 90”W x 34” D x (27”- 39”) H (height adjusted by operator).
Dimensions: BioProtect III-LE
Outside:
116.88”W x 50.00”D (FOOT PRINT) x 92.50”H (over junction boxes)
Usable work area: 102”W x 36.5”D x 65”H (under diffuser)
Cart worksurface: 90”W x 34” D x (27”- 39”) H (height adjusted by operator).
Weights
Unit weight: The weight of the BIOPROtect III cabinet is approximately 2300 lbs.
Unit weight: The weight of the BIOPROtect III-LE cabinet is approximately 1950 lbs.
Cart weight: The weight of the BIOPROtect III cart is approximately 500 lbs.
Maximum worksurface capacity: 500 Lbs. uniform load.
Point loads not to exceed 125 Lbs. each
Utilities:
Mechanical (Optional)
Four (4) 3/8” NPT female connections can be provided for plumbing; all are pre-plumbed to the top of
the unit using 3/8” copper tubing. No service fixtures or valves are provided. User must supply fixtures
to mate with 3/8” NPT female connection on side walls.
Electrical
Caution: This cabinet is powered from more that one power source. The cabinet is not electrically safe unless
all power sources are disconnected. Use proper Lock Out / Tag Out procedures.
All electrical wiring to this cabinet should comply with the National Electrical Code and any applicable Local
Electrical Codes at the site of installation.
The standard BIOPROtect III/-LE has three junction boxes located on the front of the top deck. The
following services need to be connected to the junction boxes.
One 220Volt, 20Amp, 60 Hz, Single phase connection to power the two blowers. This circuit should be
wired using 12 AWG wire and provided with a 20 Amp circuit breaker that will serve as a disconnect
for this power supply.
One 115 V, 15Amp, 60 Hz, Single phase connection for control power and power to the left side wall
duplex outlet. This circuit should be wired using 14 AWG wire and provided with a 15 Amp circuit
breaker that will serve as a disconnect for this power supply.
Page 4 of 60
One 115 V, 15Amp, 60 Hz, Single phase connection for power to the right side wall duplex outlet. This
circuit should be wired using 14 AWG wire and provided with a 15 Amp circuit breaker that will
serve as a disconnect for this power supply.
The circuit breakers for these services are to be located in close proximity to the cabinet.
Other power connections may be required for optional services to power auxiliary equipment. These
optional connections may include additional 115 Volt services up to 20 Amps and/or 220 Volt or 208
Volt services Single phase or Three phase up to 10A. When optional services are provided they are to be
wired with the appropriate size conductors and will be connected to an appropriately sized circuit breaker
that will serve as the disconnect for that power source.
The BIOPROtect III/-LE incorporates solid-state ‘Stedivolt’ controllers to adjust the blower motor speed.
This unit compensates for normal variation in incoming line voltage. You will need to consult the most
recent certification test report on the unit to determine the voltage setting should be for your unit.
Environmental Conditions
The BIOPROtect III/-LE is designed to be used under the following conditions:
Indoor use
Altitudes up to 2000 meters
Temperature range from 5° C to 40° C
Relative humidity up to 80%
Main supply voltage
115V AC ±10% for 120V circuits
220V AC ±10% for 220V circuits
Maximum relative humidity 80 percent for temperatures up to 31°C decreasing linearly to 50 percent
relative humidity at 40°C.
Transient over voltages according to Installation Category (OVERVOLTAGE CATEGORIES) II per UL
61010-1, 2nd Edition
Pollution Degree 2
Symbols and Terminology
Protective Earth: Any terminal which is intended for connection to an external protective
conductor for protection against electric shock in case of a fault.
!
General Caution: Refer to the instruction manual for information regarding personnel and
environment protection.
Page 5 of 60
II - PREPARING THE BIOPROtect III/-LE CABINET FOR USE
The Uses of the BIOPROtect III/-LE.
The BIOPROtect III/-LE cabinet has been designed to provide a work area that protects the experiment
from the environment (Product protection), and protects the users from the experiment (personnel protection
or ‘containment’). The laminar flow biological safety cabinet is designed for work with Biosafety Levels 1,
2 and 3 (low to moderate risk) agents as listed in The Center for Disease Control’s “Biosafety in
Microbiological and Biomedical Laboratories”, U.S. Department of Health and Human Services, Public
Health Service, Centers for Disease Control and National Institutes of Health, U.S. Government Printing
Office, Washington, D.C. 20402. HHS publication number (CDC) 84-8395.
Extremely high risk agents assigned to Biosafety level 4 should never be used in this cabinet except in a
certified BSL4 suit room. Please consult your safety professional for a proper risk assessment.
Cautions
* The use of any hazardous material in the cabinet requires that it be monitored by an industrial hygienist, safety
officer or other qualified individual.
* Explosive or flammable substances should never be used in the cabinet unless a qualified safety professional
has evaluated the risk.
* If hazardous biological work is to be performed, apply the appropriate biohazard decal which is enclosed. This
is in accord with OSHA regulations, volume 39, number 125, Part II.
* If chemical, radiological or other non-microbiological hazards are present, be sure to employ appropriate
protective measures in addition to formaldehyde decontamination. Call upon a suitably trained individual to
monitor the operation.Also refer to section 2 of this manual the section entitled “Decontamination”
Location Within the Laboratory
PROPER PLACEMENT WITHIN THE LABORATORY IS ESSENTIAL
.
The ideal location for any laminar flow biological safety cabinet is in a dead-end corner of the laboratory
away from personnel traffic, vents, doors, windows or any other sources of disruptive air currents. Published
research from The Baker Company (see Rake ASM paper, reference #34 in Appendix) and unpublished tests
performed at the National Cancer Institute show that if a draft or other disruptive air current were to exceed
the intake velocity of the cabinet, then contamination can enter the work area or escape from it.
Page 6 of 60
III - PROPER CABINET USE
Caution:
If the proper use of the cabinet is not understood or the cabinet is not operated correctly it
will not provide an adequate personnel or product protection.
The BIOPROtect III/-LE is designed for continuous operation. The Baker Company recommends continuous
operation to keep the interior work space clean and free of unwanted particles. If the blower is not running,
the workspace of the cabinet will become contaminated with room air. It would be helpful for operators to
learn about the capabilities and limitations of the cabinet by reading some of the available literature. The
Eagleson Institute offers several videotapes and an interactive CD which illustrate proper operation
techniques for biological safety cabinets.
A biological safety cabinet is a valuable supplement to good sterile technique, but is not a replacement for
it. All activities that are to be performed in your cabinet should first be approved by a competent
professional, such as an industrial hygienist or safety officer. This will ensure the cabinet is appropriate for
the work it will be required to do. A competent professional should monitor the cabinet and its operating
personnel at regular intervals to see that it is being used correctly.
Operator Controls:
The operator controls and indicators are arranged on the front panel of the unit.
Blower On / Off Switch –
This push button is a maintained action switch that controls the power to the cabinet blowers.
Pressing and releasing the push button from the UP/OFF position will change the switch to the
DOWN/ON position. The cabinet blowers will start. The GREEN indicator in the push button will
illuminate. Pressing and releasing the push button from the DOWN/ON position will change the switch
to the UP/OFF position.
Caution:
The cabinet audible alarm is disabled when the Blower switch is in the OFF position
Light On / Off Switch –
This push button is a maintained action switch that controls the power to the cabinet fluorescent
lights. Pressing and releasing the pushbutton from the UP/OFF position will change the switch to the
DOWN/ON position. The cabinet lights will illuminate. The GREEN indicator in the pushbutton will
illuminate. Pressing and releasing the pushbutton from the DOWN/ON position will change the switch to
the UP/OFF position.
Mute switch This push button is a momentary action switch that temporarily mutes the audible alarm. A timer
module inside the canopy controls the length of time that the alarm is muted. This is set at 5 minutes as a
factory default but can be changed to 1 second to 100 hours. It may be desirable to increase this setting
when cabinet decontamination is being performed. The YELLOW indicator in the push button will
illuminate while the alarm mute function is active. Should the alarm condition be remedied before the
mute ‘times-out’ the timer will be reset and the mute indicator will not be illuminated.
Page 7 of 60
Left Side & Right Side Outlet On / Off –
A separate 10 amp circuit breaker/switch is supplied for the remote switching of power to the right
and left outlet receptacle located inside the BIOPROtect III/-LE .The breaker switches will ‘trip’ if the
total electrical load on the circuit exceeds 10 amps.
Ground Fault Circuit Interrupter
Ground fault circuit interrupter (GFCI) outlets are installed on the 115V power circuits on this
cabinet. The GFCI is designed to protect the operator from a possible electrical ground fault hazard. If a
hazardous condition exists the GFCI will cut off electricity to the outlet. The button in the center of the
unit will pop out. If the GFCI “trips” you should first determine and correct the cause of the problem,
then press the button to reset the GFCI.
Caution: The cabinet audible alarm is disabled when the Blower switch is in the OFF position
Air Pressure Alarm
The Air pressure alarm is controlled by a Modus Air Pressure indicator mounted in the control
canopy. Operating & set up instructions for this instrument can be found in the On-Site Checks And
Maintenance section of this manual. When either a High or Low flow condition is present for more than
3 seconds the audible alarm will be activated. Pressing the Mute switch will silence the alarm. The mute
time is determined by the setting of a timer inside the control canopy. Factory setting of this timer is 5
minutes. Consult the test report for your cabinet for the factory set flow conditions for this alarm. The
Baker Company recommends continuous operation to keep the interior work space clean and free of
unwanted particles.
This feature allows the user to turn-off the cabinet blowers when no longer required and the processes
within the cabinet are found to be safe to personnel and the environment.
Caution: It is recommended that prior to turning the Blower OFF you contact your health and safety officer for
decontamination procedures.Also refer to section 2 “Decontamination”
Page 8 of 60
Door Operations
During cabinet operation, the only access that should be permitted is through the 8” sash openings
provided at the front of the cabinet. For set-up purposes, the viewscreen or the large front doors can be
opened to provide greater access.
To open the viewscreen:
Caution: Failure to slide the viewscreen in the “lock open” position may result in injury
Grasp the acrylic panel and pull it away from the door to disengage the two grab catches on each side of
the viewscreen.
Swing the panel all the way up and to the right to engage the support brackets on the cabinet’s hinges in
the “lock open” position. To close the viewscreen lift the acrylic panel off its support brackets and slide it
to the left. Lower the viewscreen to the closed position and engage the grab catches to secure it.
To open the doors (the right side door must be opened first):
Using the lever actuated door pulls, pull the right side door open, then the left.
Removing the cart
Caution: It is recommended that prior to cart removal you contact your health and safety
officer for decontamination procedures Also refer to section 2 of this manual “Decontamination”.
Should it be necessary to remove the cart, you must open the front doors to fully expose the
BIOPROtect III/-LE’s interior work bay. This area is assumed to have contamination so use caution in the
way you approach the task.
•
•
•
•
•
•
Decontaminate and clean the unit as recommended by your health and safety officer.
Open the doors to expose the cabinet’s work bay.
Any equipment on the cart should be disconnected from the side wall service connections and removed
from the cable ports.
Unlock the front casters by pulling up the foot operated locking devices. When the locking device is in a
horizontal position it is unlocked.
Use the hand crank to lower the height of the work surface if necessary. Check that your equipment will
clear the front header if it is allowed to be left on the cart.
Pull the cart out and away from the BIOPROtect III/-LE. The casters provided are swivel and all are
locking.
Page 9 of 60
Set-up Procedure
This section outlines the tasks required to properly install automated laboratory work stations within
the BIOPROtect III/-LE. This does not replace the recommendations of the equipment manufacturer. It is
assumed that the user is familiar with the set-up and operation of the ancillary equipment.
Place the equipment on the cart’s table top so that the equipment is within the area of the table top as
defined by the four corners. The front of the equipment must face the front of the cart (crank handle
side).
Using the crank handle provided on the cart, adjust the cart’s height until the work table of the equipment
is flush or below the intake grill section of the doors. Stow the crank handle under the work table.
Make utility connections to the side wall services.
Close the front doors of the BIOPROtect III/-LE and make certain that you can perform any manual
operations on the equipment through the 8” x 42” sash openings provided.
Make certain that no part of the equipment blocks or hangs over the front intake grilles.
Power and run all equipment. Make certain that the equipment and cabinet is operating properly and that
none of the BIOPROtect III/-LE’s circuit breakers have ‘tripped’.
Have a certifier or safety officer witness the air currents (using a smoke tracer) to determine that the
equipment does not compromise the cabinet’s ability to provide personnel and product protection. The
user may choose to use a particle counter to evaluate the product protection capacity of the cabinet. A
measurement of the personnel protection capacity of the cabinet (containment), should the user deem it
necessary, will be a more involved test and will require a third party skilled in the operation of the
BIO-analog test or the KI-discus test.
Page 10 of 60
Start-up procedure
• If the unit has not been left running continuously, press the blower on/off switch. The switch will be
illuminated when switched on. The cabinet’s audible alarm may activate for several seconds until the
blowers have come up to full speed. Make sure that you have cabinet airflow, either by listening for blower
sounds or feeling the airflow across your fingers. Check the reading on the air pressure gauge. The reading
on the gauge should be consistent with the last time the unit was on.
• Turn on the fluorescent lights.The switch will become illuminated in the ‘on’ position and the
fluorescent bulbs should activate.
• Wipe down the interior area of the cabinet with a surface disinfectant.
NOTES:
•
Some disinfectants may corrode or stain the steel surfaces. If this happens, clean the
surfaces afterward with a disinfectant-detergent and rinse with sterile tap water to prevent
corrosion.
•
Blocking the front and rear perforated grills must be avoided. If wipes or absorbent
towels are used on the work surface, be sure to keep them away from the grills.
•
After your equipment is in place inside the cabinet, close and secure the doors. All operations inside
the BIOPROtect III/-LE must be carried out through the unit’s 8” sash openings.
Caution: The BIOPROtect III/-LE will not provide adequate personnel protection unless the front doors and
viewscreens are closed.
•
•
After a procedure has been completed, all equipment which has been in contact with the research
agent should be enclosed, and the entire surface decontaminated.Trays of discarded items should be
covered. The cabinet should then be allowed to run for at least three minutes with no activity so that the
airborne contaminant will be purged from the work area.
After you have removed all materials, culture apparatus, etc., decontamination of the interior surfaces
should be repeated.Check the work area carefully for spilled or splashed nutrient which might support
bacterial growth. And never use the cabinet to store supplies or laboratory equipment.
Page 11 of 60
Reacting to Spills
Caution: An Emergency spill containment and clean-up procedure should be established prior to an accident
Spilled fluid on the floor of the BIOPROtect III is contained within the stainless steel work area of the
cabinet via the threshold placed under the unit’s door. The threshold will contain a volume of approximately
2 gallons (6 liters) of fluid. However, because the BIOPROtect III cabinet bears directly on the users floor
there is no drain provided.
• It is recommended that the researchers, in coordination with their consulting safety professional, have a
written plan available in case of an accidental exposure or spill.
• In the case of biological spill, for example, the area containing the spill may be flooded with an
appropriate disinfectant. After the disinfectant has had time for a complete kill, remove the residue. If you
have used a disinfectant which is harmful to stainless steel (Hypochlorite bleach solutions, for example) be
sure that none remains to corrode cabinet surfaces. Clean the surfaces with sterile water.
• The Center for Disease Control has published “Biosafety in Microbiological and Biomedical
Laboratories.” If you have a spill involving a hazardous Biosafety Level 2 or 3 agent, then you are advised
to leave the cabinet running so as to let the aerosols settle before you start cleanup procedures. With some
spills, it may be necessary to decontaminate the room with an agent such as formaldehyde gas. (Biosafety
Level 4 agents should never be used in the type of cabinet.)
• If the spill contains volatile liquids which generate vapors creating a danger of fire or explosion, turn off
the unit and other electrical equipment. Evacuate and seal the room and call for immediate help from a safety
professional.
• If the agent is a hazardous chemical, it may be recommended that a Spill Kit be kept readily available.
This kit should be clearly labeled, and might include such items as a respirator, chemical splash goggles, two
pairs of gloves, two sheets of absorbent material, spill control pillows, a solution to clean the contaminant,
and waste disposal bags or other containers. Consult your safety professional for proper procedures and
treatment of the specific agents you plan to use.
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Decontamination
Whenever maintenance, service or repairs are needed in a contaminated area of your cabinet, the unit
must first be decontaminated by an appropriate agent. The National Institute of Health, National Cancer
Institute and the Center for Disease Control have all recommended the use of formaldehyde gas for most
microbiological agents. Its application requires individuals who are experienced in the decontamination of
cabinets, since the gas itself is toxic. Consult the NSF/ ANSI-standard-49-2002 Annex G for details and
procedures on the decontamination of Biological Safety cabinets.
Whatever gas you choose, have the proper safety equipment (gas masks, protective clothing, etc.) within
easy reach. In addition, you will want to be sure that the gas you are using will be effective against all of the
biological agents within the cabinet. When you have decided which gas to use, post the antidote to it in a
visible and nearby location.
The volume of the BIOPROtect III cabinet is approximately 150 cubic feet (140 cubic feet in the /-LE),
this will help you determine the required amount of decontaminating gas.
Carcinogens present a unique chemical deactivation problem and the standard biological decontamination
will not be effective against chemicals or other non-biologic materials. With materials of this kind, consult a
qualified safety professional.
Warning! This procedure should be performed by qualified technicians only.
Decontamination procedure
Surface-disinfect the inside of the windows and all other surfaces on the viewscreen assemblys.
Multiply the total volume of the cabinet for the BIOPROtect III (150 ft3 ) by .3 gram/ft3 ) to determine the
amount of paraformaldehyde required to decontaminate the cabinet. If the cabinet is vented to the outside
you must consider the volume of the duct work in the paraformaldehyde calculation.
Prior to sealing up the cabinet make sure all gas or flammable petcocks are closed and pressure tight.
The inside cabinet work space must be at room temperature with 60% to 85% relative humidity. If relative
humidity is low (below 60%) add boiling water in the work space. Monitor the inside of the cabinet with a
humidity meter. Relatively humidity above 85% will require extra clean up which will be covered in step 15.
Note:
Without the proper relative humidity the formaldehyde gas will not be effective. The mode of entry of
formaldehyde into the living organisms is through the cell wall by the absorption of water.
Place a heating mantle with paraformaldehyde in the work space. The heating mantle must be able to reach
450 degrees F and must have a grounded plug which should be plugged in to an outlet outside the cabinet.
Place a second heating mantle in the cabinet with 60% more by weight of ammonium bicarbonate and 10%
more if using ammonium carbonate, than paraformaldehyde. This will be used later in step 13 to neutralize
the formaldehyde gas.
This step is recommended. Place spore strips inside the cabinet to confirm the decontamination process has
been successful.
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If the cabinet is vented to the room, use a sheet of plastic and seal the front access and exhaust port openings.
These openings should be sealed such that the exhaust airflow recirculates back to the front access opening.
This will promote the even distribution of formaldehyde gas throughout the cabinet.
If the cabinet is vented to the outside, then the exhaust transition should have a means to recirculate the
exhaust airflow back to the cabinet blower if possible. This will promote the even distribution of
formaldehyde gas through the exhaust filter. Seal the front access opening.
Turn on the heating mantle containing the paraformaldehyde flakes.
After 25% of the paraformaldehyde has depolymerized, turn on the cabinet blower for 10 to 15 seconds.
Repeat after 50%, 75% and 100% of the paraformaldehyde has depolymerized, approx. 20 to 30 mins..
Allow the formaldehyde gas a minimum residence time of 6 hours, preferably over night.
Turn on the heating mantle containing ammonium bicarbonate (NH4HCO3). After 25% of the ammonium
bicarbonate has depolymerized turn on the cabinet blower for 10 to 15 seconds. Repeat after 50%, 75% and
100% of the chemical has depolymerized to neutralize the formaldehyde. Turn on the cabinet blower and
allow the two gases to circulate for at least one hour.
Check for formaldehyde by using a gas sampling device (dregger tube) to be sure the formaldehyde is at a
safe level inside the lab space.
Cut open the plastic and vent the cabinet to the outside.
Aseptically remove spore strip and place in Trypticase-soy broth and incubate for 7 days. No growth will
verify the decontamination process.
When cleaning up, you may find residual paraformaldehyde (white powder) on the metal or glass surfaces.
To remove this, use ammonia in warm water, wear gloves and wipe down the affected surfaces. The
paraformaldehyde will dissolve in water and be neutralized by the ammonia.
About the HEPA Filters
The HEPA filter is one of the essential components of a biological safety cabinet. It is the shield which
stands between the environment and the experimental agent. Some questions that the user should ask
regarding the use of chemical agents in the BIOPROtect III/-LE are as follows:
• Are these chemicals, either singly or in combination, able to attack filter components?
• Are these chemicals potentially toxic to the operator? Is there any combination of two or more which
could be toxic? If the cabinet is being correctly used and only the operator’s hands and arms are inside the
machine, then toxicity or irritation could occur through skin penetration. A proper evaluation of toxicity
must deal not only with onetime exposure, but also with the effect of many small exposures over a period of
time.
• Are these chemicals explosive or flammable? If so, they should never be used in your cabinet. With a
buildup caused by recirculation of air, an explosion can be the result of a motor spark or a burner operation
in the work area.
• In cases where chemical carcinogens, mutagens or teratogens are to be used, the risks should be
carefully weighed in choosing a cabinet. Where the exhaust effluent contains a contaminant, it may need
treatment.
• The life of a filter is determined by how it is used and how often. Under normal laboratory conditions,
you can expect up to five years of use. However, misuse or a heavy dust load within the cabinet will shorten
any filter’s lifetime. Bunsen burners and misuse of chemicals will also shorten the useful life.
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IV - ON-SITE CHECKS AND MAINTENANCE PROCEDURES
We recommend that the filter leak checks be performed before initial use, after relocation and after each
filter change.They should also be carried out at regular intervals, usually six months or one year, as specified
by an industrial hygienist, safety officer or other qualified person.
As reported earlier in this manual, each individual cabinet made by The Baker Company is carefully
tested before it leaves the factory.Your copy of the test report, which you will find at the back of this manual,
gives the factory test results for your BIOPROtect III/-LE cabinet.
Use it as your record of the original testing, and as your guide to testing in the future.To gain many years
of satisfactory service, please be sure that your maintenance personnel come as close as possible to
duplicating these original test figures. Your test procedures should be identical to ours so that comparing test
results will have meaning. Please correspond directly with us to request detailed procedures for your
particular cabinet model.
Filter Media and Seal Leak Tests
When preparing your cabinet for use after shipment, and then at prescribed intervals throughout its
working life, you will need to verify that the filters have maintained their integrity.This is done by scan
testing the filter faces and seals.The Institute of Environmental Sciences recommended practice IES-RPCC034.1 would be a good reference for this testing.
The equipment needed will be:
•
•
An aerosol photometer. The instrument should sample air at a flow rate of 1 CFM.
A (D.O.P) generator with Laskin nozzle(s). Liquid dioctylphthalate (DOP), dioctylsebacate or
comparable substance aerosolized by flowing air through the nozzle(s).When generated with
Laskin type nozzle(s), the mean droplet size of the aerosol is 0.1 –3.0 micron with an average of
0.45 micron.
•
Procedure for filter leak tests
• Decontaminate the cabinet in accordance with NSF/ANSI standard-49-2002 and your health and safety
officer.
• Remove the front upper panels by first removing the ¼-20 hardware. Remove the damper from the front
of the filter. This will expose the exhaust filter.
• Find the 1/8” NPT hose barb on the top of the cabinet used for measuring the upstream concentration of
challenge.
• Turn on the aerosol photometer and set-up according to the manufacturer's instructions.
• With the cabinet’s blowers running, position the D.O.P. generator so as to introduce air generated smoke
into any of the work bay openings (side wall to door openings or rear wall openings are all appropriate for
introducing challenge). Measure the upstream concentration of the challenge. You must have a
concentration of at lease 10 micrograms per liter.
• Supply Filter: Remove the diffuser prior to scanning the filter. Holding the photometer probe no more
than one inch from the filter face scan the entire surface area and perimeter (filter gasket frame area) in
slightly overlapping strokes at a traverse rate of not more than ten feet per minute.
• Exhaust filter: You may scan the downstream face of the exhaust filter from outside of the cabinet.
• Eliminate leaks in the gasket frame area by re-tightening the filter gasket.
• An unacceptable leak is defined as a reading greater than 0.01%
Page 15 of 60
The Airflow Balance
! Warning !
This procedure should be performed by qualified technicians only.
The airflow balance which is set at the factory provides your unit with the air volume and velocity control
to minimize leakage or airborne contamination either in or out of the work area.
In order to duplicate as closely as possible the airflow characteristics described in the original factory test
report, please follow these steps:
1. Make certain that the front doors and viewscreens are closed and secured so as to make two 8” x 42”
horizontal access openings.
2. Using a DIM (Direct Intake Measurement) on each 8” X 42” horizontal access opening at the same time,
measure the flow rate into each of the front access openings. Take at least five (5) consecutive readings per
side. Average the readings and divide by 2.3 square feet (area of each front access opening). Acceptable
range of the average shall be 100-110 FPM.
3. Using a hot wire anemometer mounted on a ring stand within the work area, take readings with the
anemometer probe set 6” below the diffuser. The location of these readings should be the same as shown in
the factory test report. The average of these velocity readings must be in the range between 50 and 70 FPM.
4. After you have compared your figures with those originally taken at the factory, make whatever
adjustments are necessary. Use the motor speed controls and the exhaust dampers to bring the intake and
downflow average values within the specifications.
! Warning !
When the light canopy is lowered, some electrical parts are exposed. Do not perform this work unless you are a
qualified and trained electrician or electrical technician.
! Warning !
Terminals M1 and M2 as well as L1 and L2 are High Voltage
5. As the HEPA filters load up with particulates, airflow will be maintained automatically to some extent.
When the air flow is diminished you will have to increase the motor speeds to maintain the specified intake
and downflow velocity averages. This is done via the speed controllers located in the light canopy.
On the speed controller, turn the potentiometer clockwise until you have the desired airflow. Use a
voltmeter to check the voltage across terminals M1 & M2. This is the voltage out to the motor. If the airflow
cannot be maintained, it will necessary to replace the HEPA filters.
(See “Procedure for HEPA Filter Replacement” later in this section.).
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Airflow Smoke Pattern Test
Airflow smoke patterns have been shown to vary depending on the equipment that is used inside the
cabinet. The BIOPROtect III/-LE has been tested with the cart only. A description of this testing is
summarized in the test report. However, when the BIOPROtect III/-LE is set-up properly (i.e. your set-up
should not block or restrict the grilles and diffusers provided) the appearance of the airflow directly at the 8”
access openings must be consistent with that described in the test report.
To check for the direction of air movement, use a smoke generator and trace along the front access
opening on the inside of the cabinet. Observe that no smoke is escaping from the work area.
In order to be sure that room air is not entering the work area, trace along the outside of the front access
opening. Observe that no smoke penetrates to the work surface.
Grounding Continuity Test
Using an Ohmmeter, set it to read the low resistance scale.Touch the two leads together and see that the
display reads 0.1Ω to 0.0Ω. Touch one lead to the ground lug on the cabinet power cord while touching the
other lead to bare metal on the unit where the user would be likely to touch the cabinet. According to NSF/
ANCI-standard 49-2202 the maximum allowable shall be 0.15Ω (Max allowable for UL is 0.50Ω ).
Page 17 of 60
Calibration procedure for Modus Airflow alarm(Digital Differential Pressure Monitor).
Note: This procedure MUST be followed every time the damper width has been changed.
Initial Set Point Settings
Balance the cabinet air flows to nominal operating set point per manufacturer instruction.( See
instruction label on front of cabinet).
Record voltage to motor/blower (M1, M2 on speed control) and measure and record damper/ slot
width
Make certain that the Modus displays 0.000 when zero pressure is applied(This can be easily
accomplished by removing the Tygon tubing from the hose barb fitting). Use the “zero” function if
necessary.
Re-connect the tygon tubing and make sure that the value displayed is negative(-) when the cabinet is
running.
Low Flow Alarm Setting
Place a DIM airflow hood on the front of the cabinet or use the 3 inch method for measuring inflow.
Adjust cabinet to a 20% reduction in airflow by decreasing the voltage to motor. DO NOT adjust the
damper
Record the exhaust/ damper pressure on Modus (indication of exhaust plenum pressure at
20% reduction) and voltage to motor/ blower (across M1, M2 on speed control)
Set the Modus High Alarm set point to the 20% reduction in pressure recorded in step 14 (per Modus
programming instructions below)
Set the Modus dead band to .002 (per Modus programming instructions below).
Return cabinet to Nominal Set Point voltage, recorded in step 2
Check the Calibration
Set Point Check
While measuring the intake airflow with a DIM, reduce the airflow by decreasing the voltage to
motor/ blower until a 20% reduction in flow is measured. Check that the low set point alarms at this
value. If not, reset Modus low alarm set point to alarm as desired, per instructions. Return cabinet
to Set Point Voltage.
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Modus Programming Instructions
1.) Programming the Alarm Set points
After the first press of MODE, the HI LED flashes, indicating the HI set point is being entered. The
least significant digit (right hand) should be flashing. Press the ARROW key to scroll to the desired
digit (0 through 9). Press the MODE to move towards the most significant digit and press the
ARROW key set each remaining digit’s value. The sign of the set point (+ or -) is selected last.
After the HI set point has been programmed, press the MODE to enter the LO set point. The LO
alarm LED flashes, and you can repeat the above process to program the LO set point.
2.) Programming the Relay Dead band
The dead band selection is provided to keep the relays from chattering when the pressure varies near
the set point. To display the dead band, press MODE once after setting the LO set point polarity in
the previous step. Both alarm LEDs flash. The three digits displayed are the dead band width, in the
same units used for data display. Press the MODE and ARROW keys as described earlier to set the
dead band to a value of .002.
3.) Returning to Normal Operation
Press MODE once after the setting the dead band parameters. The digit “1” on the left indicates the
unit is in analog setting mode. Since there are no analog outputs, the MODE key can be pressed a
final time to end programming and return to the normal pressure display.
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Maintenance Notes
WARNING!
Before any panels are removed, the cabinet must be decontaminated.Also see section 2 “Decontamination”
The filters are sure to have collected microorganisms and other potentially harmful particles generated from the
work area during their lifespan. Maintenance personnel should not allow themselves to be exposed.
It should also be remembered that a specific gaseous decontaminant may work against microorganisms, but
not against chemical agents. Where chemical agents are present, consult an industrial hygienist or other qualified
person(s).
Replacing the HEPA Filter
If the differential pressure monitor presents an alarm, or if your periodic checks of total airflow show a
drop of more than 5% of the nominal set point, the filters may be loading with particulates. As explained
earlier in this section, the blower speed can be manually increased to compensate for filter loading.
However, when the airflow can no longer be maintained or when the filters are damaged, they need to be
replaced.
A contaminated filter must be handled with caution. Personnel should be protected by clothing or
breathing apparatus as necessary to the nature of the hazard. It is advisable to seal the contaminated side of
the filter by taping a plastic sheet or cardboard over the face before removal. This should minimize the
number of particles shaken loose from the filter. Once removed, the filter should immediately be sealed in a
biological hazard bag and then disposed of safely in accordance with environmental regulations.
After filter replacement has been completed, the cabinet and the room should be cleaned and
decontaminated in a manner consistent with the nature of the hazardous material. The cleaning materials,
along with the protective gear and clothing, should be properly disposed of.
HEPA filters are very easily damaged, and you will want to use great care during handling so as to avoid
injury to the filter media and gasket surface. When installing the new filters, it is a good idea to tape a piece
of cardboard over the filter media to give protection against dropped wrenches or misdirected fingers. Of
course, you will need to make sure that the cardboard is removed before the access panels are re-installed.
Inspect the filters carefully before and after installation. A broken or damaged filter is worthless.
Changing the Exhaust Filter
The BIOPROtect III/-LE requires two exhaust filters measuring 15” x 38” x 12” deep (consult test
report for part numbers and specifications).These filters are arranged to face the front of the unit so as to
eliminate the nuisance of ceiling restrictions on the exhaust air.
To service the exhaust filter remove the panels from the front of the unit using a 3/8” nut driver or
socket to remove the ¼-20 hardware. Using the same 3/8” driver, remove the hardware from the filter
securing frame. Pull the frame and filter away from the unit.
Prepare the new filters by putting a light coat of silicone grease on the face of the gasket. Clean the
sealing flange thoroughly. Then carefully slide the new filter into place and make sure that it is properly
seated on the flange. Replace the filter clamp assembly and perform the leak test described earlier in this
chapter.
Page 20 of 60
Changing the Supply Filter
The BIOPROtect III/-LE requires two supply filters measuring 32” x 45” x 3” deep (consult test
report for part numbers and specifications). These filters are arranged so as to be serviced from inside the
BIOPROtect III/-LE work area. Consequently you will have to remove the cart and any equipment from
inside the cabinet prior to changing the filters.
To service the supply filters first swing the diffusers down allowing them to rest on the rear wall by
removing the ¼-20 screws at the front edge of the diffuser panel. Next using a 3/8” driver remove the ¼20 hardware that secures the frame to the filter. This frame supports the filter.
Prepare the new filters by putting a light coat of silicone grease on the face of the gasket. Clean the
sealing flange thoroughly. Place the new filter in the center of the frame and install the frame into the
cabinet using the ¼-20 hardware.Conduct a filter leak check as described in the earlier chapter.
Page 21 of 60
V. - TROUBLE SHOOTING
Here are some suggestions based on our experience with the use and misuse of biological safety
cabinets.
CAUTION:
Whenever the potentially contaminated areas of your cabinet must be entered, make sure that the unit is first
decontaminated by use of an appropriate method by qualified personnel.Also see section 2 “Decontamination”
When a smoke test indicates that there is air flowing from the interior of your cabinet into the
surrounding room:
• Make certain that the front doors are closed and the front intake grilles are not blocked. This will create
8”x 42” horizontal openings in the front of the cabinet.
• If your cabinet is connected to an in-house exhaust, make sure that there is adequate exhaust suction and
no back pressure. Also, be sure the dampers are open. Re-balance the exhaust system to handle an adequate
quantity of air and static pressure (suction). Consult with building maintenance people.
• The exhaust filter may be loaded with dirt if the unit has been in service for some time. Decontaminate,
and replace the HEPA filter.
• There may be high cross-drafts in the room which are causing the outflow of smoke. Check the airflow
balance, following the procedure recommended in an earlier section of this chapter. Eliminate the source of
the cross-draft.
If there is no airflow within the work area:
• Make sure that the blower switch is turned on. The indicator on the push button should be illuminated.
• If the lights are working, then turn the blower switch off and let the cabinet rest for ten minutes. When
the time has passed, turn the blower switch on again. If the blowers start, you know there has been
overheating of the blower motor(s).Also check the wiring connections inside of the light canopy which
connects the canopy to the cabinet. Be sure the connections are pushed together.
• If these solutions do not correct the problem, or if the blowers failed to start after the rest period, then
either the speed control, blower motor(s) or capacitor is defective. A qualified electrician can find out if the
speed control is defective by bypassing it, using the wiring diagram in the Appendix of this manual as a
guide. If there is a noise problem, it may be caused by motor bearings.
When there is uneven fan operation, or noise from the motor/blower assembly:
• It may be necessary to decontaminate the cabinet.
• Look for loose objects in the fan cage.
• Check to see if the fan wheel is contacting the blower housing.
• Open the light canopy and check the multi-pin connectors at each end to be sure they are securely
engaged.
• Another possible source of the problem is the speed control.
Page 22 of 60
If the fluorescent light does not work • Make sure that the light switch is turned on.The indicator on the pushbutton should be illuminated.
• Make certain that the bulbs are installed properly into the clips.
• Open the light canopy and check the multi-pin connectors at each end to be sure they are securely
engaged. If the lamp flickers, there is probably an improper ground.The wiring can be traced to the source of
a break.
Page 23 of 60
REPLACEMENT PARTS LIST
PART NAME
QTY
BAKER COMPANY
PART NUMBER
Blowers
2
11432
Capacitor
2
11558
Motor (220 V, ½ HP, 1620 RPM)
2
11629
Filter, exhaust, 15” x 38” x 12”
2
41319
Filter, supply, 32” x 45” x 3”
2
38913
Power Supply, 24V DC, 1A
1
41452
Motor speed control
2
39658
Fluorescent lamp
4
41386
Ballast, fluorescent
2
39391
Pressure Monitor/Alarm
1
38996
Hydraulic pump kit (cart)
1
40187
NOTE:
When ordering replacement parts, please furnish serial number and model number of unit
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VI. APPENDIX
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Modus Installation and Operation Handbook
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BGA-BIOPROtect III Vent to Room Page 1
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BGA-BIOPROtect III Vent to Room Page 2
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BGA-BIOPROtect III Vent to Room Page 3
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BGA-BIOPROtect III-19 Hard Exhaust Connection(HEC) Page 1
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BGA-BIOPROtect III-19 Hard Exhaust Connection(HEC) Page 2
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BGA-BIOPROtect III-19 Hard Exhaust Connection(HEC) Page 3
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BGA-BIOPROtect III-20 Canopy Exhaust Connection (CEC) Page 1
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BGA-BIOPROtect III-20 Canopy Exhaust Connection (CEC) Page 2
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BGA-BIOPROtect III-20 Canopy Exhaust Connection (CEC) Page 3
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BGA-BIOPROtect III-LE Vent to Room Page 1
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BGA-BIOPROtect III-LE Vent to Room Page 2
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BGA-BIOPROtect III-LE Vent to Room Page 3
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BGA-BIOPROtect III-LE Hard Exhaust Connection (H.E.C.) Page 1
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BGA-BIOPROtect III-LE Hard Exhaust Connection (H.E.C.) Page 2
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BGA-BIOPROtect III-LE Hard Exhaust Connection (H.E.C.) Page 3
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BGA-BIOPROtect III-LE Canopy Exhaust Connection (C.E.C.) Page 1
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BGA-BIOPROtect III-LE Canopy Exhaust Connection (C.E.C.) Page 2
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BGA-BIOPROtect III-LE Canopy Exhaust Connection (C.E.C.) Page 3
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BGA-BIOPROtect III/-LE Optional Cart
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Wiring Diagram-Standard BIOPROtect III/-LE
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WARRANTY
The Baker Company, Inc. expressly represents and warrants all goods (a) to be as specified in the Baker
Company catalogs and literature, and (b) to be free under normal use, service and testing (all as described in
the Baker Company catalogs and literature) from defects in material and workmanship for a period of twelve
months from the invoice date.
The exclusive remedy for any breach or violation of this warranty is as follows: The Baker Company,
Inc. will, F.O.B. Sanford, Maine furnish without charge repairs to or replacement of the parts or equipment
which proved defective in material or workmanship. No claim may be made for any incidental or
consequential damages.
This warranty is expressly in lieu of all other warranties, express or implied, including any implied
warranty of merchantability or fitness for a particular purpose, unless otherwise agreed in writing
signed by the Baker Company. (The Baker Company shall not be responsible for any improper use,
installation, service, or testing of the goods).
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