PROCTOR VERSION 2.5 B: Energy Structure of Cells Quiz 1. A planarian has a mutation that affects mitochondria. The planarian moves slower and has a slower rate of growth and reproduction than a normal planarian. A mitochondrion from a normal planarian (planarian X) and a mitochondrion from the planarian with the mutation (planarian Y) are shown below. Which statement best explains why planarian Y moves, grows, and reproduces at a slower rate than planarian X? (A) The mitochondrion from planarian Y does not have a large enough surface area to absorb food and oxygen to produce ATP. Distractor Rationale: This answer suggests the student may understand that the inner mitochondrial membrane of planarian Y has less surface area, but does not understand that the inner mitochondrial membrane does not directly absorb food, or that the rate at which oxygen is used is reduced because the reduced surface area of the inner membrane reduces the number of places where the reactions that produce ATP can occur. (B) The mitochondrion from planarian Y does not have enough space in the matrix to store enzymes needed to generate ATP through the oxidation of organic molecules. Page 1 of 6 PROCTOR VERSION 2.5 B: Energy Structure of Cells Quiz Distractor Rationale: This answer suggests the student may understand that organic molecules are oxidized in the mitochondrial matrix, but does not understand that the space inside the matrix is larger in planarian Y, and that the reduced surface area of the inner membrane means there are fewer places where the reactions needed for ATP production can occur. (C) The mitochondrion from planarian Y does not have a large enough surface area to hold electron-transferring proteins and is unable to adequately oxidize NADH and FADH2 to generate enough ATP. Rationale: This answer suggests the student understands that the folding of the inner mitochondrial membrane increases its surface area, which allows more ions to be pumped into the intermembrane space and produce an electrochemical gradient, and that this allows for more rapid production of ATP during cellular respiration because there is more surface on which reactions can occur. (D) The mitochondrion from planarian Y does not have the ability to pump H+ into the intermembrane space, and is unable to produce ATP through chemiosmosis. Distractor Rationale: This answer suggests the student may understand that H+ storage is needed to generate ATP and that ATP is produced through chemiosmosis, but does not understand that only the rate at which H+ is pumped into the intermembrane space is affected, or that the reduced surface area of the inner membrane means that there are fewer places where the reactions needed for ATP production can occur. Aligned to: LO 2.5 CA 2.5: Explain Energy Use, Storage & Capture Page 2 of 6 PROCTOR VERSION 2.5 B: Energy Structure of Cells Quiz 2. Cyanide is introduced into a culture of cells and is observed binding to a mitochondrion, as shown in the diagram below. The following observations are made: • Cyanide binds to and inhibits an enzyme in the inner mitochondrial membrane. • There is a decrease in water production in the inner mitochondrial membrane. • Cyanide causes a build-up of electrons in the form of NADH. Which statement best explains how cyanide disrupts cellular processes? (A) Cyanide prevents organic molecules from entering the mitochondrion. Distractor Rationale: This answer suggests the student may understand that organic molecules (pyruvate) must enter the mitochondrion, but does not understand that cyanide primarily disrupts the electron transport chain by binding to enzymes that accept or donate electrons along the electron transport chain. (B) Cyanide prevents enzymes from splitting glucose in substrate-level phosphorylation. Distractor Rationale: This answer suggests the student may understand that the splitting of glucose in substratelevel phosphorylation involves enzymes and produces energy-rich molecules that drive the electron transport chain, but does not understand that the splitting of glucose occurs outside the mitochondrion in the cytosol and is not affected by the presence of cyanide. Page 3 of 6 PROCTOR VERSION 2.5 B: Energy Structure of Cells Quiz (C) Cyanide prevents the production of carbon dioxide by binding to the enzymes involved in the citric acid cycle. Distractor Rationale: This answer suggests the student may understand that carbon dioxide is produced during the citric acid cycle, but does not understand that cyanide primarily disrupts an enzyme in the electron transport chain from accepting or donating electrons and does not disrupt the citric acid cycle directly. (D) Cyanide prevents the oxidation of energy-rich molecules by binding to enzymes that facilitate movement of the electrons in the electron transport chain. Rationale: This answer suggests the student understands that the location of the electron transport chain is in the inner mitochondrial membrane, and that cyanide binds to enzymes in the electron transport chain, preventing them from accepting and donating electrons in the electron transport chain, which then prevents the formation of an electrochemical gradient needed to power the production of ATP via chemiosmosis. Aligned to: LO 2.5 CA 2.5: Explain Energy Use, Storage & Capture 3. The diagram below shows the pathway for the movement of electrons in a mitochondrion. Page 4 of 6 PROCTOR VERSION 2.5 B: Energy Structure of Cells Quiz Which statement describes the process in chloroplasts that is most similar to the process shown in the diagram? (A) The reduction of carbon dioxide into 3-carbon sugars in the stroma during the Calvin cycle Distractor Rationale: This answer suggests the student may understand that the reduction of carbon dioxide to 3carbon sugars involves the transfer of electrons, but does not understand that electrons are not transferred in a way that is similar to the movement of electrons down an electrochemical gradient in the electron transport chain. (B) The excitation of electrons in photosystem II on the thylakoid membrane during the lightdependent reaction Page 5 of 6 PROCTOR VERSION 2.5 B: Energy Structure of Cells Quiz Distractor Rationale: This answer suggests the student may understand that electrons are excited by light in photosystem II, but does not understand that electrons are excited to higher energy levels in photosystem II, rather than moving to lower energy levels as they move through the electron transport chain. (C) The movement of hydrogen ions from a high concentration inside of the thylakoid space to the stroma via chemiosmosis Distractor Rationale: This answer suggests the student may understand that hydrogen ions move from the thylakoid space to the stroma during chemiosmosis in chloroplasts, but does not understand that the movement of hydrogen ions in the diagram is from regions of low concentration to regions of high concentration (the electron transport chain) to produce the electrochemical gradient that is then used to drive the production of ATP (chemiosmosis). (D) The oxidation of proteins on the thylakoid membrane during noncyclic photophosphorylation to create an electrochemical gradient Rationale: This answer suggests the student understands that the electron transport chain on the thylakoid membrane is similar to the electron transport chain on the inner mitochondrial membrane and that the energy released by the transfer of electrons through the electron transport chain via redox reactions is used to pump protons across a membrane, which produces an electrochemical gradient that provides energy for the production of ATP (chemiosmosis). Aligned to: LO 2.5 CA 2.5: Explain Energy Use, Storage & Capture Page 6 of 6