The Dolomite Centre Ltd
Dolomite Microfluidics – North America Office
Unit 1, Anglian Business Park, Orchard Road,
Royston, Hertfordshire, SG8 5TW, UK
Blacktrace Inc, 29 Albion Place
Charlestown, MA 02129, USA
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+44 (0)1763 242491
+44 (0)1763 246125
sales@dolomite-microfluidics.com
www.dolomite-microfluidics.com
617 803 6655
617 848 1121
617 500 0136
salesus@dolomite-microfluidics.com
Resealable Chip Interface
Part No. 3000305
product datasheet
Description
Benefits
Specifications
Chip Design
Resealable Chip Interface Geometry
Chip Geometry Information
Standard Chip Options
Custom Chip Options
Parts and Accessories
The Dolomite Centre Limited
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Description
The Resealable Chip Interface is used in combination with microfluidic chips that can be
opened and resealed. The chip base layer is loaded into the interface first followed by the
gasket and then the chip top layer. The interface clamps the layers together and provides
eight fluidic and eight electrical connections to the chip. For standard resalable chips, the
gasket is attached to the chip top layer.
Reagents, sensors, biosensors or cells can be deposited on the chip base layer. The chip
base layer material is typically glass, quartz, or polymer. Fluids are then flowed through
channels in the gasket layer over the deposited sensors or reagents. The depth of the
channels in the gasket is typically between 100 – 500 microns.
Applications include: biosensor testing, cell culture and analysis, dielectrophoresis
experiments, impedance detection, and interfacing with silicon chips and sensors.
Left: Resealable Chip Interface (Part
No.3000305) without a chip
Below: Resealable Chip Interface with a
custom chip and gasket
Benefits
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Low profile allows viewing with a microscope
Excellent chemical resistance
Easy to clean
Eight fluidic and eight electrical connections
Allows deposition of reagents, sensors or cells onto channel surface
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Specifications
Connector Specification
Value
Number of Fluid Connections
8
Number of Electrical Connections
8
Chip size
29.8mm x 22.3mm
Chip top layer thickness
3.0mm
Chip base layer thickness
2.0mm
Gasket Thickness range
100 – 500µm
Outside diameter of fluid connection tubing
1.6mm (1/16inch)
Inside diameter of fluid connection tubing
0.25mm, 0.5mm, 0.8mm
Fluid connection tubing material
PTFE (FEP, PFA or PEEK)
Wetted Materials
PEEK, PTFE, Viton
Interface Diameter
62.0mm
Interface Thickness
12.0mm
Maximum Operating Pressure
3.0bar*
* This is based on a test with Top Chip, 4-in, 1-out (3200072) and a Blank Base Chip (3000343).
Maximum Operating Pressure may vary for custom chip designs.
Chip Design
The windows at the top and bottom of the holder allow a microscope objective lens to get
within millimetres of the microfluidic channel structure. The exact distance depends on the
channel size. Details are shown below:
Microscope objective
Top chip layer
Base chip layer
Gasket layer
Microscope objective
Custom chips can be designed and fabricated by Dolomite. There are two types of
gaskets available as shown below:
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Gasket integrated with top layer
Separate through-cut gasket layer
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Resealable Chip Interface Geometry
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Chip Geometry Information
Location of fluid holes in Top Chip layer
Location of contact pads for
electrodes in Base Chip layer
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Standard Chip Options
Dolomite can supply a range of standard top chips and base chips for the resealable chip
holder. Available standard chips:
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Manual Injection Top Chip (Part No. 3200069)
Manual Multi-Injection Top Chip (Part No. 3200070)
Top Chip, 1-in, 1-out (Part No. 3200071)
Top Chip, 4-in, 1-out (Part No. 3200072)
Top Chip, 3-in, 1-out (Part No. 3200120)
Large Well Base Chip (Part No. 3000478)
Blank Base Chip (Part No. 3000343)
For further information on these chips, please view the website.
Custom Chip Options
Custom chips can also be designed and supplied including top chips in PMMA or glass
with integrated PDMS gaskets. Base chips can be supplied with a thin patterned metal
layer on the top surface (Platinum is typically used however other metals are also
possible). The metal thickness is typically 100 – 200nm and the minimum feature size on
the pattern is typically 2µm.
It is also possible to customise the base of the resealable chip holder to integrate silicon
chips or PCB mounted components. This allows fluidic and electrical interfaces to be
made with a wide range of devices.
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Parts and Accessories
Resealable chip interface top
Resealable chip interface
manifold with seals
Resealable chip interface PCB and
electrical connector and cable
Linear Connector (Part No.
3000024)
Resealable chip holder base
The base also comes in a smaller size for use with thinner chips.
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