MACSQuant Analyzer optical bench
525/50
FL-2
555 LP
585/40
FL-4
655 LP
488/10
SSC
Laser 488 nm
FL-7
495 LP
FL-3
Laser 635 nm
FL-6
FL-1
750 LP
450/50
730 LP
630 LP
Laser 405 nm
Objective
FL-5
FSC
Pinhole
Cuvette
Optical parameters of the MACSQuant Analyzer
Excitation (nm)
FL channel
For the detection of:
405 (violet)
FL-1
VioBlue™ /
Hoechst / Pacific Blue™
488 (blue)
FL-2
FITC / GFP
FL-3
PE
FL-4
PI / PE-Cy5.5 / PerCP
FL-5
PE-Cy7
FL-6
APC
FL-7
APC-Cy7
FSC / SSC
Forward and side scatter
635 (red)
488 (blue)
MACS is a registered trademark of Miltenyi Biotec GmbH; MACSQuant, Vio and VioBlue are trademarks of Miltenyi Biotec GmbH.
Pacific Blue is a trademark of Molecular Probes, Inc. Cy is a trademark of GE Healthcare UK Ltd.
The optical bench of the MACSQuant
Analyzer has three lasers (violet, blue
and red). The three lasers are spatially
separated and are focused on the
cuvette in three different locations.
As the cells flow through the cuvette
one at a time, light is scattered and
collected at 180° (FSC) or is deflected
through an objective at 90° angle (SSC
and fluorescence signals). The SSC and
fluorescence signals are separated by
various filters and are collected in the
appropriate photomultiplier tubes (PMTs).